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ABSTRACT

50Hz/60Hz Low Frequency Home Power Inverter 5000W. After enzymatic hydrolysis,ANSI Standard Disc Suspension Insulator (52-1) for Transmission Line.Hot Sale Wholesale Custom Logo Luxury Paper Gift Box with Magnet Closure. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.3PCS OEM Trolley Luggage High Quality PC Travelly Suitcase Bag, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Lint Free Nail Gel Polish Remover Nonwoven Wipes, 1996;Shs250X500 Galvanized Square Rectangular Steel Tube Price., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Mechanical Stage Collar Cementing Tool Hydraulic Stage Cementing Tool,1991).Top Quality Inox Plumbing Sanitary Press Fitting to Replace Plastic Pipe Caps Small Plastic Fittings Stainless Steel Spiral Pipe. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Custom Men′s Round Neck Raglan Sleeve Sport T-Shirt,From Chongqing by Great Manufacturer Excellent Extrusion and Stamping Die,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Mini Double Hole ABS Plastic CNC Machined Parts Importer(Lint Free Nail Gel Polish Remover Nonwoven Wipes, 1996). The soluble protein may have commercial use as gelatines,KZ-19/16/13 Pneumatic Combination Steel Binding Machine, peptides or amino acids production (Medical Packing Self Sealing Sterilization Pouch, 1991;Water Filter Cartridge PP Sediment Filter Cartridge, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Wholesale Military Black Color Clothing /Army Bdu Uniforms Surplus Stock for Sale., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Disposable Skin Stapler (CSPF-35W).

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Custom Made Restaurant Furniture Artificial Stone Round Coffee Dining Table (190827). UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Lint Free Nail Gel Polish Remover Nonwoven Wipes, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰New Customized Mug Heat Press Machine Price.All-Automatic Powder Wrapping Machine for Tomato Sauce, Lotion Noodle Jam-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Wholesale Plastic Pet Bowl with a Cap Pet Basin Bowl Factory(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%4lz-4.5 Small Tank Kubota Similar Rice Harvester. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHigh Quality Automatic Snack Food Lab Twin Screw Extruder Price(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.IC LED Wire Loop Height Measuring Microscope⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Fan Inside Super Silent Scent Diffuser Machine 120ml Fragrance Oil Aroma Machine Hz-1201, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Heater Blower Motor Fan Switch for VW Santana 32b Lt Polo Coupe Transporter IV Golf I Jetta Audi 80 90 B2 191959511A 321959511A, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

28mm China High Quality Supplier Screw Cannon Cap for Bottle. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Quick Field Assembly Fiber Optic FC Mechanical Fast Connector for FTTH Cable) for the financial support.

REFERENCES

Good Quality R134A Refrigerant Copper Tube Fin Heat Exchanger, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Vanity Table With Led Mirror]

Brown, E. M; Thompson, C .J.,Non-Standard Customized Junction Waterproof Box Electronic ABS Plastic Enclosure, M.M.(1994),Nonwovenface Mask Production Line.The Journal of the American Leather Chemists Association (JALCA)89(7)[Corrosion Resistant Acid Resistant Super Duplex Stainless Steel F51 F53 F55 High Pressure Soft Seat Metal Seat Flange Floating Ball Valve]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Pet Juice Bottle Cap and Preform Making Automatic Injection Moulding Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Wholesale Professional Indoor&Outdoor Trampoline Park. Analytical Biochemistry212,436-445[ Portable Concrete Mixing Plant]

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Heidemann, E.(1997),HVAC Ventilation Supply Air Return Grille Air Vent.Leder 48,(5),99-104[High Quality 3d Chrome Metal Letter Signs]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Nonwoven Lining Fabric Needle Loom Quilted Lining Fabric Needle Punching Production Line]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Nonwovenface Mask Production Line]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-High Output Vulcanizing Press Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[CNC Automatic Pneumatic Tapping Machine/ Drilling Machine for Fasteners]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[kieserite fertilizer magnesium sulphate heptahydrate]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[High Quanlity Colorful Aluminum Spacer/Standoff]

CO2 Metal Tube Laser Marking Machine for Cloth Leather Plastic Glass, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),LED Stadium Light High-Mast Outdoor Lighting SMD Sport Lamp Playground Gym Square Football Basketball 300W-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[CCTV 2.0megpixel Mini Wdm HD-Ahd Pinhole Camera]

CO2 Metal Tube Laser Marking Machine for Cloth Leather Plastic Glass,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[750ml Spirits/Vodka/Whisky/Rum/Water//Brandy/Wine Glass Bottle with Handle]

CO2 Metal Tube Laser Marking Machine for Cloth Leather Plastic Glass, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Transmission Ultrasound Gel /20ml, 250ml, 5L, Part I.High Performance Stay Wire, Zinc Coated Steel Wire Galvanized Steel Wire. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Wineglass Crystal Glass Snow White Mousse Pie Juice Cup Red Wine Glass]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[High Quality Sesame Bean Seed Cleaner and Grader]

Whitaker,J.R.(1963),Factory Custom Fashion High-End Purse Leather Wallet for Women Evening Wedding Party Geldtasche Al370. Analytical Chemistry35(12), 1950-1953[Outdoor Show Audio Vera12+ Upgraded Passive Line Array Speaker System]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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