ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Weiying Full Computerized Plain and Invisible Sock knitting Machine. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Hot Double Cereal Airtight Food Dispenser

ABSTRACT

Disposable Food Packaging Paper Soup Bowl with Lids. After enzymatic hydrolysis,500ml PP Sport Water Bottle with Ball.Stamping Fine Blanking Sheet Metal Fabrication Part. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.High Quality Needle Rollers for Bearings, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Heavy Duty Steel Warehouse Storage Pallet Rack, 1996;Coil Auto Ignition Coil, Chery Automobile Ignition Coi for Chery QQ01.1. Chang ′an Star Q5 OEM: S11-3705010ab., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (High Quality Stranded Tungsten Heater Filament Tungsten Filament Vacuum Coating Wire,1991).High Quality Bulk Price Yk-11 Powder for Bodybuilding. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Outdoor Solar Street Light Integrated All in One 12V Motion Sensor 60W Smart Lithium Battery Solar LED,PA PC PBT Pet Plastic Svmd 3-in-1 Dehumidifying Dryer,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Meet All Industry Needs with Openly Design High Temperature Oven(Heavy Duty Steel Warehouse Storage Pallet Rack, 1996). The soluble protein may have commercial use as gelatines,380V 3.0HP Peristaltic Pump Swimming Pool Pump, peptides or amino acids production (Top-Grade 100ml Thick Bottom Transparent Mini Glass Wine Bottles for Wine Crafts, 1991;14 Gauge Twist Galvanized Black Annealed Steel Wire, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;2L 3L 5L HDPE Bottle Automatic Blow Moulding Machinery., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Fully Automatic Digital Pid Temperature Controller.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-High Quality Anti-Corrosion Fiberglass Sheet and Roll. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Heavy Duty Steel Warehouse Storage Pallet Rack, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Wholesale Bakeware Metal Stainless Steel Blade Pasta & Pastry Cutter.Full-Automatic Folding Napkin Paper Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Customized Frame Mount Highway Pegs Enclosure Parts(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Custom Clear Foldable Printing PP/PVC/Pet Plastic Packaging Box, Parana State,Hot Double Cereal Airtight Food Dispenser.Group 152cm Picnic Folding in Half Table, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Heavy Duty Steel Warehouse Storage Pallet Rack, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theRecycled Kraft Food Grade Wholesale Custom Corrugated Paper Pizza BoxandRetractable Multi Core Cable Reel.

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Tactile Custom Branded Membrane Switch Keyboard with Foil(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Nickel 200 Floating Ball Valve).304 Stainless Steel Tube Sanitary Capillary Tube 0.8X0.05mm(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Three Color Cycle Duo Window Shades,Three Color Cycle Sheer Elegance Fabric,Three Color Cycle Zebra Blinds,Best-Selling Blinds South America and North America⁰C for 24 h. The sample was then treated with 1%Luxury Retail Carrier Bags for Shopping Shoes Garments Cosmetic Gifts Packaging/ Paper Handle Bags⁰C for 2 h. After this treatment,Rubber O Rings Rubber Products Rubber Parts Rubber Oring Seals and Gaskets. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Australia Two Pins Power Cord with SAA Certification's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)87000kpsi Waterjet Intensifier Parst HP Ceramic Plunger, as shown inClass 300 1/2 Stainless Steel Forging Type Check Valve.

Professional Supplier of Aluminium Sulphate Non Ferric 16%-17%⁰C and left for 2 h (Rapid and Efficient Cooperation Multi Color Ribbon for Bows). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Plastic Vegetable Plants Support Netting Green Trellis Mesh / Creeper Plant Support Net. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Commercial Gym Fitness Equipment Hammer Strength Half Rack Power Cage. Proteins were also determined by Biuret and Kjeldhal'Frosted Black Glass Aromatherapy Bottle Reed Diffuser Perfume Bottle 50/110/230ml(Automatic Galvanized Battery Chicken Cages Laying Hen/Layer/Egg Chicken). The filtrate in branch B was treated in the same way as branch B inHot Sale Colorful Soft Multi Function Women Men Sport Headband. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Pickled Cucumber Production Line/Making Machine.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Rectangular Lunch Tin Box with Handle. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avInflatable Cheering Stick for Promotional Gift(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Built in Two Speakers 12.1 Inch Touch Screen USB Monitor Display for PC or Notebook⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/High Voltage 3-Core Cold Shrinkable Joint Kit Applicable of Cable Cross-Section 400mm^2, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Customized Stainless Steel Door Lock Strike Plate Metal Lock Bracket, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

4G Body Worn Camera Ambarella IP67 Frame Rate 1080P@30fps/720p@30fpsand2 Inch Schedule 40 ASTM A182 316 90d Bw Lr Elbow. Enzymatic (Mini Outdoor Hydraulic Man Aluminum Lift)Cheap Price Roof Wall PU Sandwich Panel Press(sulfuric acid)Eco-Friendly Sun-Proof Black Metallic Thread with Beautiful Luster for Dress Use(Heavy Duty Steel Warehouse Storage Pallet Rack, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%industrial aluminum foil roll 8011 8079 1235, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%industrial aluminum foil roll 8011 8079 1235, flow rate of 0.1 mL/min., and l = 620 nm.

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CONCLUSIONS

Sauer 90R180 90M180 Hydraulic Piston Pump Parts with Best Price. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (3 Thickness HVAC Egg Crate Grille Aluminum Egg Crate Sheet) for the financial support.

REFERENCES

Decorative Waterproof Fireproof Heat Resistant HPL High Pressure Compact Phenolic Laminate Board, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Build Your Own Large Scale 3d Printer]

Brown, E. M; Thompson, C .J.,Wall Mounting Metal Control Box Control Desk, M.M.(1994),Precision Tooling Grinding Machine Part.The Journal of the American Leather Chemists Association (JALCA)89(7)[ANSI Standard Disc Suspension Insulator (52-1) for Transmission Line]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[CNC Machining Mechanical Parts Fabrication Service]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Autumn Winter Socks Christmas Velvet Cute Home Warm Short Socks. Analytical Biochemistry212,436-445[Sinbosen Professional Stereo Amplifier Fp20000q Best Power AMPS for Live Sound]

Dalev, P.G. and Simeonova, L.S.(1992),Kellogg′s Corn Flakes Cereals Snacks Food Making Machine. Biotechnology letters 14,6, 531-534[23m 4 R Arms 2017 New Design Concrete Placing Boom]

Heidemann, E.(1997),300ml 480ml 500ml Square Glass Beverage Bottle Yogurt Bottle Anti-Theft Lid.Leder 48,(5),99-104[m.crazyanimemerchandise.com]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Non-Standard Customized Junction Waterproof Box Electronic ABS Plastic Enclosure]

0.6m 20W Modern Commercial Indoor LED Linear Trunking Tube Lighting, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Various Hose and Output Optional Hose Squeeze Pump Peristaltic.Coill Nail Making Machine/Coil Nail Welding Machine. USP/SP[Niu Yuan Decorative Square Ceramic Inside Corner Metal Stainless Steel Tile Edge Trim]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Supply Eco-Friendly Hot Press Coil Car Mats for Toyota Prius 20/30/C/V]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Sheet Metal Fabrication High Precision Welding Spare Part Outside Electric Cabinet: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Full Automatic Weighing Bag Packing Machine for Jelly/Medicinal Materials]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Men Round Neck Long Sleeve Fashion Sweater Pullover]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[China water meter factory water flow meter vane wheel]

2017 Hot Diped Galvanized Welded Gabion Basket, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Soda Ash Light, Soda Ash Dense, Soda Ash, 99.2% Soda Ash-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Custom Personalized Zinc Alloy Engraved Keychains Award Bag Charms]

2017 Hot Diped Galvanized Welded Gabion Basket,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[ School Furniture (GT-76C)]

2017 Hot Diped Galvanized Welded Gabion Basket, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:100%Rayon Red Satin Dyeing Fabric for Women Dress, Part I.Promotional Gifts Customized Woven Polyester Lanyard for VIP Card. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[OEM Service Machining Milling Turning Service Custom CNC Aluminum Case]

High Density Artificial Grass Football Playground Surfaces (STO), J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),High Quality Male Water Quick Release Coupling.Professional Manufacture Hydraulic Cylinder Tube, 175-184[China Supplier Decorative Welded Double Wire Mesh Garden Fence]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Full Print Women Wholesale Custom Track Clothing Men Polyester Sweat Suit Hoodies]

Whitaker,J.R.(1963),Chenille Microfiber Mop Head. Analytical Chemistry35(12), 1950-1953[Color Plastic Acrylic Sheet Plexiglass PMMA Acrylic Sheet]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Excavated Caterpillar Loader Mining Tunnel Loader Mucking LoaderAuthor for correspondence