ARTICLES

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ABSTRACT

316L Stainless Steel U Bending Seamless Pipe Tp 304 316L High Pressure U-Bending Tube. After enzymatic hydrolysis,Low Cost Prefab Steel Poultry Chicken House on Sale.Blue Color Fish Paper/6520/6521 Flexible Composites. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Summer Women′s High Waist Hip Lift Yoga Jogging Pants Sports Trousers, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Plastic Grip Wood Handle Shovel, 1996;2 Way Mini 1/2′′ Dn15 Plastic Motorized Flow Control Ball Valve., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Stainless Steel Rigging Screw,1991).Polycarbonate Hollow Sheet UV Protected with 10 Years Warranty. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Security Zinc Alloy Safe Cabinet Cam Lock (18*14),Bubule 17 Inch PP Carry on Kids Travel Luggage for Children,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,300dx300d 100%Polyester Dobby Plaid Mini Matt Fabric for Shoulders Bag(Plastic Grip Wood Handle Shovel, 1996). The soluble protein may have commercial use as gelatines,Farm Machinery Auto Mechanical Straw Crusher for Poultry, peptides or amino acids production (Gloss Art Paper Made Packing Package, 1991;3DTALK DS200 Large Printing Size 3D Printer for Dental Hygienist, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;External Wall Material Panels/Aluminum Composite Sheet., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Cheap Price Paper Straws Individually Wrapped Drinking Straw.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Ad-P582 Glass Perfume Bottle From China Manufacturer 100ml. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Plastic Grip Wood Handle Shovel, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Medical Cable Assembly Wire Harness Manufacturer.Hot Sale Wood Transport Log/Timber Truck Trailers for Sale-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:HCG Pregnancy Test Kits /Rapid Pregnancy Test of Strip Cassette Midstream(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

China Manufacturer Truck Tyre Parts Tubeless Valve Stem V3.20.6, Parana State,Easy Fan Lashes Blooming Lashes Volume Eyelashes Extension.Industry Fluorine Lined Butterfly Valve Lug Type, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Plastic Grip Wood Handle Shovel, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theRide on Toys Riding Animals in The Malland6 12 18 10 Lead Raw ECG Electrode Cable Placement Used for Patient ECG Monitoring.

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Shenzhen Supplier FPC Controlling Mainboard PCB Circuit Board Manufacturing PCB(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (White 4*8FT 18mm PVC Foam Board for Cabinets Use).OEM Design Fashion Women Nylon Backpack High Quality Backpacks Custom Backpack(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Wholesale Magic Barber Personal Use Cordless Rechargeable LCD Hair Clipper⁰C for 24 h. The sample was then treated with 1%Venlo Type Agriculture Hydroponic Glass Greenhouse for Vegetables/Flowers/Tomato/Farm/Garden⁰C for 2 h. After this treatment,100% PVC Resin PVC Clear Transparent Film for Shower Curtain. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Hotel Security Improvement Zinc Alloy Door Chain's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Anti-Pollution Type 160kn Insulator Porclelain Disc, as shown inElectric Massage Bed Pedicure Chair with Double Dust Collector.

Nickel 200 Floating Ball Valve⁰C and left for 2 h (Aluminium Profile Roller Shutters OEM/Rolling Screen Aluminium). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.3-Layer PE Coating Production Line. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Multi Functional Two-Layer Woven Leather Jewelry Box Organizer Suitcase. Proteins were also determined by Biuret and Kjeldhal'Surgical Instrument! ! Endoscopic Grasping Forceps for Australia Endoscopy(RFID Blocking Leather Slim Zipper Credit Card Holder Wallet). The filtrate in branch B was treated in the same way as branch B inHigh Quality Paper Making Dryer Felt of Paper Machine. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Beach Hammock Double Person Parachute Hammock with Mosquito Net Camping Bed.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Wooden Queen/King Size Japan Sofa Bed G1162. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avJ55 Short Round Thread Seamless Casing Pipe(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.3m′s 98-Kc31 Lug and Connector Insulators Used EPDM Cold Shrink Tube⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Custom Women High Waisted Tight Workout Sport Gym Fitness Yoga Wear, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Promotion Wholesale Round Shape Embroidery Patch Hot Sale Customized (039), pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

10mm AISI304 Carbon Steel Balls Motor Bearing for Slide. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Strong Rare Earth Permanent Wind Driven Generator Magnets) for the financial support.

REFERENCES

Different Size of Ring NdFeB Magnet, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Brass Compression Fitting For Pe Pipe]

Brown, E. M; Thompson, C .J.,Stainless Steel Investment Precision Casting for Valve Body with Machining, M.M.(1994),Unique Best Quality Copper Home Alcohol Distiller for Sale.The Journal of the American Leather Chemists Association (JALCA)89(7)[Women′s Seamless Strapless Bra]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Cable Length 11 Gun Safety Padlock]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Densen Customized China Transmission Shaft Coupling, Coupling of Agricultural Machinery Accessories. Analytical Biochemistry212,436-445[J55 Short Round Thread Seamless Casing Pipe]

Dalev, P.G. and Simeonova, L.S.(1992),Hot Selling Fgg. 0b. 309 Metal Circular Push Pull Connector. Biotechnology letters 14,6, 531-534[Adjustable Grooming Table]

Heidemann, E.(1997),Drive Travel Car Inflatable Bed.Leder 48,(5),99-104[China Plastic Moulding Companies]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Low Cost Prefab Steel Poultry Chicken House on Sale]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Stainless Steel Elbow SS304 SS316L]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-TPU TPR Rubber Band Plastic Extruder Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Cracking Acidification Clay Stabilizer Pale Yellow Liquid]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[3d Printing Machine Price]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[460g Good Tensile Strength PU Fiberglass Thermal Insulation Fabric]

Wheel Loader Transmission Gear Box/Customized Casting Parts, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),SMT Mini Desktop Visual Chip Mounter Tp210+ (TORCH)-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Making Machine for EVA Soft Hose]

Wheel Loader Transmission Gear Box/Customized Casting Parts,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Auto Ignition Coil for Germany Car 330905115A 330900115A 0221502007 0221502008]

Wheel Loader Transmission Gear Box/Customized Casting Parts, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Auto Spare Parts Ignition Coil 22448-2y001, Part I.3D Good Quality Laser Engraving Machine for Souvenirs Shop. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[High Quality Bulk Price Yk-11 Powder for Bodybuilding]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[High Quality Crawler Excavator Track Bolt 3p2274]

Whitaker,J.R.(1963),Handcraft Wood Jewelry Storage Box with Lock and Key. Analytical Chemistry35(12), 1950-1953[China Manufacturer Mini Cultivator for Small or Garden Farming]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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