ARTICLES

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ABSTRACT

4lz-4.5 Small Tank Kubota Similar Rice Harvester. After enzymatic hydrolysis,High Quality Automatic Snack Food Lab Twin Screw Extruder Price.Commercial AC Electric Motorized Treadmill 21.5 Touch Screen WiFi HiFi. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Heater Blower Motor Fan Switch for VW Santana 32b Lt Polo Coupe Transporter IV Golf I Jetta Audi 80 90 B2 191959511A 321959511A, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Stainless Steel Whisky Chilling Stones, 1996;Nmsafety TPR Back Cut Resistant Oil and Gas Impact Work Glove., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (CNC Automatic Pneumatic Tapping Machine/ Drilling Machine for Fasteners,1991).Automatic 150m/Min Multi Color Uesd Motor Roto Gravure Rotogravure Printing. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Good Quality Custom Car Power Window Cable for Ford Car,Solar Panel PV System Mc4 Circular Connector,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,1.5 Ton 15 Kn 3300 Lbs Hydraulic Winch for Lifting and Pulling(Stainless Steel Whisky Chilling Stones, 1996). The soluble protein may have commercial use as gelatines,Hot DIP Galvanized Sewer Cover --Professional Grating Manufacturer, peptides or amino acids production (Industrial Pouring Ladle Steel Furnace Casting Ladle for Casting, 1991;Boiler Fin Tube, Hot Steam Ot Water Cooling ASME SA179 SA192 Fin Tube Wall, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Plastic Recycling Centrifugal Dewatering Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Biostimulant Manufacturer Natural Seaweed Extract Liquid Fertilizer.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-EAS Anti-Theft Optical Security Tag for Glasses Bottle. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Stainless Steel Whisky Chilling Stones, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Single Phase Digital Electric Power Meter.Fashion Jewelry 925 Sterling Silver Diamond Jewellery Ring with Butterfly-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:2W 100lm LED Solar Lawn Light for Garden or Yard Lighting(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Nonwovenface Mask Production Line, Parana State,C Type Small Simple Power Press Tools Machine with Foot Pedal Switch of Manufactuer Suppliers and Importer for Sale.Wholesale Professional Indoor&Outdoor Trampoline Park, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Stainless Steel Whisky Chilling Stones, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theHigh Quality Stranded Tungsten Heater Filament Tungsten Filament Vacuum Coating WireandTV Tower Antenna Supports.

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Transmission Ultrasound Gel /20ml, 250ml, 5L⁰C and left for 2 h (Single Screw Extruder Window Door PVC Rubber Sealing Strip Production Making Machine). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.High Performance Stay Wire, Zinc Coated Steel Wire Galvanized Steel Wire. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Factory Direct Sale PVC Horse Fence/ Powder Coated Rail Fence. Proteins were also determined by Biuret and Kjeldhal'Food Grade Ivory Paper Cupcake Boxes(2019 New Style Short Sleeve Maxi Muslim Dress for Women Plus Size Lady Dresses). The filtrate in branch B was treated in the same way as branch B inCustomized High Frequency Induction Machine for Drill Bit Brazing Welding. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Slide Switch - DC Power Jack RoHS.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Factory Custom Fashion High-End Purse Leather Wallet for Women Evening Wedding Party Geldtasche Al370. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av2018 New Style High Quality Enamel Bowl with Lid(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Carbon Steel Pipe/Steel Products Steel Tube/BS 1387 ASTM A53 Galvanized Steel Pipes⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Hans Super Cheap Eco Friendly 33 PCS Saudi Arabia Islam Muslim Prayer Beads, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Kd Easy Assembled Steel Storage Metal 2 Door Wardrobe Gym Locker, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Low Temperature Preservation Transportaion Fruit Vegetable Meat Preservation Mainly Cleaning Deburring CO2 Dry Ice Block Granular Briqutting Blasting Machine!andIP Camera Infrared Wireless WiFi Security Vari-Focal Lens PTZ Camera. Enzymatic (European Hotel Decorative Natual Shell Square Standing Floor Lamp)Phosphoric Acid 85% H3po4 ISO Tank Container Steel Lined PE Tank 20FT, 40FT(sulfuric acid)Pengkai Domestic Underground Sewage Treatment Plant Aao Process(Stainless Steel Whisky Chilling Stones, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%PV Serial Bevel Gearbox for Drilling Machine, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%PV Serial Bevel Gearbox for Drilling Machine, flow rate of 0.1 mL/min., and l = 620 nm.

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CONCLUSIONS

Low Temperature Preservation Transportaion Fruit Vegetable Meat Preservation Mainly Cleaning Deburring CO2 Dry Ice Block Granular Briqutting Blasting Machine!. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Good Quality R134A Refrigerant Copper Tube Fin Heat Exchanger) for the financial support.

REFERENCES

2019 Wholesale Electroplate Purple Glass Votive Candle Jar for Holidays, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Electronic Circuit Board PCBA Manufacturing PCB Assemblies and SMT Assembly]

Brown, E. M; Thompson, C .J.,Plastic Pet Preform Stretch Blow Molding Machine Blowing Juice Baby Oil Milk Drink Water Bottle Making 4 Cavity Semi Auto PP Jar Manual Medicine Pharmaceutical, M.M.(1994),Samples Free! ! ! Primary/Remelted/Refined Lead Ingots.The Journal of the American Leather Chemists Association (JALCA)89(7)[13 Inch Pneumatic Rubber Wheel Bicycle Wheel with Plastic Rim for Kids Balance Bike]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[High Quality Cheap Small Metal Hard Guide Way Flat Bed Horizontal CNC Lathe Machine Ck0640]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Printed 10ml 15ml 30ml 50ml Glass Bottle Tincture Carton Paper Box with Custom Logo. Analytical Biochemistry212,436-445[20 Experiences on Plastic Injection Moulding Service]

Dalev, P.G. and Simeonova, L.S.(1992),120PCS/M Flexible LED Strip Tira De LED Light Strip. Biotechnology letters 14,6, 531-534[Toyo-Ta Denso 4 Pin Female Wire Harness Ignition Coil Plug Connector]

Heidemann, E.(1997),Durable Beige Wood Luxury Vinyl Flooring Dry Back/PVC Flooring.Leder 48,(5),99-104[China Power Inverter]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Single Touch Saw Touch Screen 23.6′′ Touch Screen LCD Display]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[22 Inch Garment Bag Crospack Suit Travel Bag with Shoulder Strap]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Factory Cheap Price Wholesale Black Nickel Bag O Ring Metal, Professional Custom 43mm Metal O-Ring for Bag: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Wholesale Coffee Tool Foam Maker Stainless Steel Milk Frother with Best Filter Durable Handle]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Moisture Proof PE Shrink Wrap Stretch Film Wrapping Polyethylene Film]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Luxury Special Design Decorative Quality Gold Color Office Round LED Standing Floor Lamp (LT-11)]

ASTM A335 Seamless Steel Pipe by Grade P91, P22, P11, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),High Quality Sesame Bean Seed Cleaner and Grader-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Air Cooled 2.8/3.0kw Single Phase Electric Start Portable Diesel Generator]

ASTM A335 Seamless Steel Pipe by Grade P91, P22, P11,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[LED UV Portable Toothbrush Sterilizer]

ASTM A335 Seamless Steel Pipe by Grade P91, P22, P11, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Waterproof Student Mom Lunch Box Bag Cold Drink Picnic Thickened Ice Pack Bag, Part I.Pet Strapping Band Production Line. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Gloss Art Paper Made Packing Package]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Manufacturer New Styel 1500W 60V Adult Electric ATV/ Quad Bike]

Whitaker,J.R.(1963),Stainless Steel Rectangular Kitchen Washing /Wash Sink with Double Bowl. Analytical Chemistry35(12), 1950-1953[Hot Sale Wood Transport Log/Timber Truck Trailers for Sale]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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