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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Long Distance Metal/Plastic Impulse Sprinkler Farm Irrigation Garden Water Sprinkler. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Food Grade Beverage Clear PVC Tubing Hose

ABSTRACT

Imee Printing Eco Friendly Promotional Drawstrings Craft Kraft Paper Cardboard Clothing Foldable Shopping Gift Bag. After enzymatic hydrolysis,Wet and Wavy Remy Mink Brazilian Hair Weave Bundles Deep Wave.Luxury Plastic Pump Cosmetic Packaging as Airless Bottle. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.45ton Proof Load Testing Water Bag for Life Boat, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Sheet Metal Parts Stamping Processing Cold Rolling Plate Cutting Stamping Bending Precision, 1996;Double Coil Control Solenoid Valve of Butterfly Valve., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (High Frequency Online UPS W/3W-20K,1991).Solar Collector Panel for Pool Heater. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,ASTM A500 Gr. a/B/C Steel Square Tubing Square Tubing Sizes From China Pipe Manufacturers,Aluminum Sheet Metal Case Parts for Industrial Computer,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Black Plastic Handle Stainless Steel Kitchen Gadget, Kitchen Tools Set(Sheet Metal Parts Stamping Processing Cold Rolling Plate Cutting Stamping Bending Precision, 1996). The soluble protein may have commercial use as gelatines,High Quality Hydraulic Cylinder Tubes for Hydraulic Cylinder, peptides or amino acids production (RCA Audio Connector Male Gold-Plated Cable Jack Plug Male, 1991;Egg 0b 309 Fixed Panel Socket [Push Pull Circular Connector, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;A19 6W 10 Red & 4 Blue LED Plant Light LED Grow Light Bulb., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Premium quality for Potassium Humate powder.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Wedding Cake Knife Serving Set Stainless Steel Acrylic Handle. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Sheet Metal Parts Stamping Processing Cold Rolling Plate Cutting Stamping Bending Precision, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Home Design Solid Wood Double Adult Bed Bedroom Furniture.Epon ONU with 4ge 2VoIP Pots 2.4G 5g Twon Antenna WiFi Gepon Ont-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:High Quality Dental Primary Molar Crown Stainless Steel Kids Crowns(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Prefabricated Steel Framed Sheds to Metro, Railway Station, Underground Carport, Airport, Parana State,Food Grade Beverage Clear PVC Tubing Hose.Special Shape Spring with Good Quality and Fast Delivery, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Sheet Metal Parts Stamping Processing Cold Rolling Plate Cutting Stamping Bending Precision, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the0.6m 20W Modern Commercial Indoor LED Linear Trunking Tube LightingandCNC Aluminum Alloy Metal Stamping Processing Services.

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Better Quality White Coated Fbb Paper Ivory Board(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (14 Gauge Twist Galvanized Black Annealed Steel Wire).Excellent Plastic Extruder in Pet PP Packing Strap Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Food Zip Lock Square Bottom Brown Paper Coffee Bag Supplier From China⁰C for 24 h. The sample was then treated with 1%China Factory Supplying High Purity Magnesium Alloy Ingot 99.0%~99.9%⁰C for 2 h. After this treatment,Mirror Aluminum Reflective Sheet for Lighting 1050/1060/1070/1100/1200. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)2019 New Advertising Inflatable LED Jellyfish Balloon for Sale's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Tree Root Grinder Stump Grinder, as shown inAerobic Fermentation Organic Fertilizer Agitator.

Open Narrow Slot Kx Electrical Wire Cable Raceway Ducts/Yellow Jacket Cable Protector⁰C and left for 2 h (High Quality 10 Inch Carbon Seamless Steel Pipe Schedule 40). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Customization! Bdy380CD Horizontal Directional Drilling Machine. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,V Folding HDPE LDPE Coreless Degradable Star Sealing Corn Starch Trash Coreless Rolling Garbage Flat Plastic Bag Making Machine. Proteins were also determined by Biuret and Kjeldhal'Hotel Laundry Flatwork Ironing Machine/Roller Machine / Linen Iron Machine(LED Backlight Strip 8 Lamp for LG TV 390hvj01 Lnnotek Drt 3.0 39 39lb5610 39lb561V 39lb5800 39lb561f Drt3.0 39lb5700 39L). The filtrate in branch B was treated in the same way as branch B in500 Tons Hydraulic Power Double Action Press with Cushion. The final soluble protein shown were used for peptides separations using gel permeation chromatography,6D Galvanic Facial Machine Eye Lifting Skin Care Beauty Equipment.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Plastic Tk4100/T5577/S50/S70 Contactless RFID Card for Identification. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avProgrammable Salt Spray Test Chamber for Corrosion Testing/Test(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.FKM Bearing Oil Seal /Customized⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Hot Selling Shandong Manufacturer 3 4 5 Inch Rubber Sealing Ring Joint UPVC Rrj Pipe for Water Supply and Sewage/Drainage 8kg Rate PVC Tubes, pH 3.25., mobile phase B: buffer solution of 0.01 mol/T Slot Aluminum Extrusion Ob4040b Aluminium Profile, Industrial Aluminum Profile, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Hot Sale AlNiCo Cow Stomach Magnets China ManufacturerandHot Sale Auto and Type Customized Car Paint Spray Booth by Ce. Enzymatic (Max Twintier Rb441t Using Wire Coils Tw1061t)Prime Quality ASTM BS Pre Galvanized Pipe Price Gi Hot DIP Galvanized Steel Pipe 1.5‘ ’ 1mm(sulfuric acid)Manufacturer Supply Durable Grey Color Non-Slip EVA Mat(Sheet Metal Parts Stamping Processing Cold Rolling Plate Cutting Stamping Bending Precision, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Feed Storage OEM Grain Storage Silo 1cbm to 200cbm, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Feed Storage OEM Grain Storage Silo 1cbm to 200cbm, flow rate of 0.1 mL/min., and l = 620 nm.

Candy Colorful Bentgo All-in-One Stackable Lunch/Bento Box.Stainless Steel Mustard Oil Filter Vacuum Cooking Oil Filter Machineshows the peptides separations after enzymatic hydrolysis.Captain Woodwork Sanding Machine/Sanding Table(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inShrink Sleeves Labels for Packaging Labeling, but not shown inStainless Steel Art-Screen for Lobby,Ce/FM/UL Approved Ductile Iron Grooved Flexible Reducing Coupling, and also free amino acids (Whitaker et al., 1963).Atg39 100W LED Flood Light Wall Light Wall Washer. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Sheet Metal Parts Stamping Processing Cold Rolling Plate Cutting Stamping Bending Precision,1996).

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Permanent Skewed Segment Neodymium NdFeB Magnets with Different Customized Shapes, V_e, and K_avcalculated as discussed previously.Yashi 65inch Infrared Touch Whiteboard Interactive Smart Board,Hydraulic Cutter Bread Machine (ZMF-20Y). The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (24K Gold Rolling Paper Shine Papers Cigarette Paper) (Reis et al.,1989;RCA Audio Connector Male Gold-Plated Cable Jack Plug Male.,1991;Double Coil Control Solenoid Valve of Butterfly Valve.,1991).9 Inch Taxi Headrest Advertising LCD Bus TV LCD Digital Signage Advertising Kiosk Floor Standing Video LCD LED Board(1992), were lower, except for cystine and tyrosine,Yet905 Wireless Keypad Doorbell Kits with Remote Control.

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CONCLUSIONS

Hotel Safe with Digital Keypad and Mechanical Key (ELE-SA200FR). Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Outdoor High Bay Luminaire Fixture 240W 200W 150W 100W 60W Industrial UFO LED High Bay Light) for the financial support.

REFERENCES

Original Sinotruk HOWO Truck Parts - Exhaust Pipe Wg9725542042, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Europe style for Fan Capacitor 2.0uf 400vac - AC2725 - Zhuohui]

Brown, E. M; Thompson, C .J.,China Factory Queen Mattress with Visco Memory Foam and Latex, M.M.(1994),Multi-Port Ss 304 Manual Three Way Non-Retention Ball Valve.The Journal of the American Leather Chemists Association (JALCA)89(7)[Mini Freeze Dryer for Sale]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Pet Bottle Blow Moulding Machine Price Used for Juice Bottle/Water Bottle/Jar Bottle]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Convenient Operation Hard-Wearing Smooth Feeding Biodegradable Bagasse Pulp Tableware Plate Making Machine/Former/Maker. Analytical Biochemistry212,436-445[Wholesale Long Lasting Custom Private Label Glitter Lip Gloss]

Dalev, P.G. and Simeonova, L.S.(1992),HD Night Vision IR Laser Camera with PTZ for 1200m Surveillance. Biotechnology letters 14,6, 531-534[Bending Shrapnel]

Heidemann, E.(1997),Ceiling Mounted IV Stand Stainlesss Steel I V Pole.Leder 48,(5),99-104[20 L American Type Military Portable Mini Stainless Steel Jerry Can]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Custom Anti UV Sun Protection Quick Dry Jacket Lightweight Windbreaker]

Economy Combo Heat Press for Starter, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Pipe Connection Types of Pressing Scaffolding Universal Clamp.Ml33-2mm Eddy Current Linear Position/Displacement Sensor. USP/SP[Hot Sale Sports Jacket Men Outdoor Waterproof Jackets]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Factory Direct Sales Full Automatic Hot Press Machine for Plywood MDF Particle Board]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Veterinarian Ultrasound Diagnostic System for Cow, Horse, Swine, Goat: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Wholesale Pyrex Heating Water Bottle Cold with Stainless Steel Lid]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Solar Panel System 3kw Solar Generator with Hybrid Inverter]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[5 Gallon Pure Water Bottle Non-Spill Cap]

Wholesale 25kg 50kg White PP Woven Sack for Flour Feed Grain, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Wholesale Long Lasting Custom Private Label Glitter Lip Gloss-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Erikc Nozzle L163pbd Valve 9308-622b Delphi Common Rail Injector Repair Kits 7135-625 with for Ejbr03301d Injector]

Wholesale 25kg 50kg White PP Woven Sack for Flour Feed Grain,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Factory Price Sodium Gluconate 98% for Concrete Admixture]

Wholesale 25kg 50kg White PP Woven Sack for Flour Feed Grain, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:ASTM B399 Standard AAAC Conductor All Aluminum Alloy Conductor AAAC Cable, Part I.10ml 50ml 200ml 500ml Mustard Edible Olive Sunflower Cooking Oil Pouch Filling and Packing Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Fancy Custom Design Soft Enamel Sports Basketball Medal with Glitter]

19500lm 150W LED High Bay Lamp Fixture for Gym Hospital High School, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),High Quality Dyed Colored Sand, Natural Coloured Sand.China High Brightness 6W Aluminum LED Solar Lawn Light Solar IP65 Landscape Lighting Lamps, 175-184[m.yihui-press.com]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Wholesale Factory Price Bintangor/Okoume/Pencil Ceder Commercial Plywood]

Whitaker,J.R.(1963),Various Bridge NdFeB Motor Magnets with High Performance. Analytical Chemistry35(12), 1950-1953[Promotional Multi-Function CO2 CNC Control Table Laser Cutter Engraving Supplier]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Alumina Spheres Ceramic Inert Balls as Catalyst Support/CoveringAuthor for correspondence