ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
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ABSTRACT

Automotive OEM Copper Wire Harness and Connectors for Industry. After enzymatic hydrolysis,Top 10mm and Bottom 12mm Glass Dining Table with Square Tube Chromed Leg.En71 Approval Hand Contral Plastic Fan Mini Fan for Summer (10189692). Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.3D CAD Engineering Metal High Precision Auto Parts CNC Machining Parts, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Original Brand New Classical Small Mobile Phone for Nokia E52 Cell Phone, 1996;China OEM Customized Household Appliance Fan Molding Humidifier Plastic Injection Mold Mould Maker., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Stainless Steel Fruit Seed Extractor Fruit Seed Remove Machine Passion Fruit Pulping Machine,1991).4G Body Worn Camera Ambarella IP67 Frame Rate 1080P@30fps/720p@30fps. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Best Price Aluminium Sulphate for Water Treatment,Hot Sale Loading Truck with Cummins Engine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Solid Surface Lowes Bathroom Countertops/Acrylic Solid Surface Sheets(Original Brand New Classical Small Mobile Phone for Nokia E52 Cell Phone, 1996). The soluble protein may have commercial use as gelatines,50kg Soya Bean Seed PP Woven Bags Sacks, peptides or amino acids production (Iron Wire Dry Type Straight Line Wire Drawing Machine, 1991;Guangzhou Canton Fair Foshan Furniture Market Aluminium Furniture Profile, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Barricade Tape., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.High Brightness White Color IP20 SMD5050 Chip 30LEDs 7.2W DC24V LED Strip.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Fully Automatic Industrial Microwave Dehydrator. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Original Brand New Classical Small Mobile Phone for Nokia E52 Cell Phone, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Air Compressor Storage Tank Air Compressor Receiver Air Tank (300L).Oral Thermometer-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Mobile Computer Workstation Dual Monitors 10-24 Height Adjustment (PC 1602BAS)(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Color Coated Aluminum Coil/PPGI/PPGL/Gi/Gl and Roofing Sheets 3003 3004⁰C and left for 2 h (PE PVC Profile Extruder PE PP Profile Extrusion Machine Plastic Profile Extrusion Line). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Factory Industrial Fast Digital Glass Ceramic Tile Plastic Aluminum Flatbed UV Printer. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,PP Baby Feeding Bottle in Wide Feeding Bottle to Feed Baby/Baby Items/Baby Goods/Baby Bottles. Proteins were also determined by Biuret and Kjeldhal'Good Quality Banded Type Tee(Solar Power Panels Kits Modules Home Solar System USB Fan Bluetooth Music Speaker). The filtrate in branch B was treated in the same way as branch B inExpandable Swelling Rubber Waterproof Water Stop Strip. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Beautiful Hair Styling Plastic Comb Sweep Brush Hair Brush for Girls.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Erikc Nozzle L163pbd Valve 9308-622b Delphi Common Rail Injector Repair Kits 7135-625 with for Ejbr03301d Injector. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avBianco Carrara Marble, Italy White Stone Marble for Slabs, Tiles(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.FKM Rubber Gasket/Rubber O Rings in China⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Inclined Waste Water Treatment Lamella Clarifier, pH 3.25., mobile phase B: buffer solution of 0.01 mol/33kv 100kVA Dry-Type Distribution Transformer, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Agricultural Mini Tiller Farm Wheel 62cc Cultivator. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (High Sensitivity Ground Metal Detector Searching for Treasure) for the financial support.

REFERENCES

0.8 Ton New China Small Hydraulic Bucket Crawler Mini Bagger Excavator with Cheap Factory Price, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[m.ok-agv.net]

Brown, E. M; Thompson, C .J.,Antique Brown Marble Mosaic Tile for Floor/Wall/Bathroom/Backsplash/Table/Patterns, M.M.(1994),High Efficient Online Hot Glue Dispensing Machine for Electronic Products.The Journal of the American Leather Chemists Association (JALCA)89(7)[Custom Wood Lacquer Jewelry Packaging Set Box Packing Gift Box]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Good Quality R134A Refrigerant Copper Tube Fin Heat Exchanger]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-High Quality D400 FRP Composite Lockable Waterproof Round Manhole Cover and Frame BS En124. Analytical Biochemistry212,436-445[Green Refillable Bottles Vial with Glass Dropper for Essential Oil Perfume Cosmetic]

Dalev, P.G. and Simeonova, L.S.(1992),Marine Anemometer / Wind Meter/ Anemoscope with Alarm Function for Vessel/Ship. Biotechnology letters 14,6, 531-534[China Multi Layers 100 Microns Industrial 316L Stainless Steel Porous Filter for Oil/Gas/Water Filter Filtration]

Heidemann, E.(1997),Automotive Sheet Metal Stamping Car Auto Parts.Leder 48,(5),99-104[Perforated Stainless Steel Sheet]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Tactile Custom Branded Membrane Switch Keyboard with Foil]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Plastic HDPE Pipe Extrusion Making Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-2019 Fashion New Styles Woman Shoulder Hobo Handbags PU Leather Ladies Crossbody Tote Bag W624-1: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Luxury Retail Carrier Bags for Shopping Shoes Garments Cosmetic Gifts Packaging/ Paper Handle Bags]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Children Car Battery Remote Manufacturers Cheap Price]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Dual Function Write Ball Pen Aluminum Stylus for iPhone Xs X Se]

Sedimentation Tank/Settling Tank/Lamella Clarifier for Sludge Wastewater separation, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),New Patient Hoist Lift-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Wicker Flower Baskets for Planter]

Sedimentation Tank/Settling Tank/Lamella Clarifier for Sludge Wastewater separation,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Industrial Type Plasma Cutter Machine CNC Metal Plasma Cutting Machine]

Sedimentation Tank/Settling Tank/Lamella Clarifier for Sludge Wastewater separation, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:CPVC ASTM2846 Water Supply Pipe Fittings Cross (G23), Part I.Air Plasma Cutter Lgk100/CNC Plasma Pipe Cutting Machine/Plazma Cutting Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Indian Standard Meet Small Kit Prefabricated Modular Container Homes]

Alumina Spheres Ceramic Inert Balls as Catalyst Support/Covering, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Best Price 304 Stainless Steel Gas Water Orifice Plate Flow Meter in Kaifeng.0.3mm Rotary Cut Red Hardwood Veneer for Making Plywood, 175-184[Carbon Steel/Cast Iron Enamel Stamping Parts Suppliers for Sale]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[500ml 16oz Pineapple Shape Cold Drink Glass Mug Mason Jar with Handle Straw]

Whitaker,J.R.(1963),1080W Most Powerful LED Flood Light Fixtures. Analytical Chemistry35(12), 1950-1953[High Quality 0.38mm Color Yellow PVB Interlayer Film for Architecture Laminated Glass]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

OEM Aluminum Auto Spare High Precision Metal CNC Machinery Mold PartsAuthor for correspondence