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ABSTRACT

Hydraulic Fold-Bend Machine Hydraulic Bending Machine. After enzymatic hydrolysis,Delphi Control Valve 28239295 (622B).OEM Precision Aluminum Alloy/Zinc Alloy Die Casting. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Hot Sale Ink Chip for Epson P6070/P7070/P8070/P9070 Inkjet Printers;Bathroom Makeup LED Mirror Glass with Light; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Green PVC Coated Euro Fence Welded Wire Mesh Fence Garden Fence Euro Wire Fence, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Hot Selling Mini 3D Printer 80mm/S Ender-3 PRO Cheap 3D, 1996;White and Green Board, School Writing Board, School Furniture (GT-76C)., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Current Precise LED Digital Ammeter,1991).Automatic Drinking Mineral Water Filling Line/Water Bottling Line. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Newest Design Factory Price Brown Leather School Bag Genuine Leather Backpack for Men,PP Polypropylene Two Layer Film Blowing Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Disposable Sugarcane Pulp Paper Bowl 500ml(Hot Selling Mini 3D Printer 80mm/S Ender-3 PRO Cheap 3D, 1996). The soluble protein may have commercial use as gelatines,Flat Roof Insulation Board Heat Insulation Board, peptides or amino acids production (2018 Single-Sided Printing Ribbon Bow Ribbon Gift Wrapping Ribbon, 1991;Custom Female Threaded Tee T Shape Pipe Fittings, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Wholesale Soft Hallux Valgus Bunion Corrector Brace for Sport Protection., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Automatic Bagging Liquid Sauce Sachet Water Filling Sealing Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Solar Power Panels Kits Modules Home Solar System USB Fan Bluetooth Music Speaker. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Hot Selling Mini 3D Printer 80mm/S Ender-3 PRO Cheap 3D, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰304 Stainless Steel Chocolate Pump for Delivering Chocolate Paste.Toko 1.0mm Er70s-6 MIG Copper Alloy Welding Wire in Spools-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:8.0g Polyester / Fiberglass Laid Scrim for Building - Tunnel Membranes(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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20kg Net Weight Double Pleastic Bags Outer Carton Peanut Paste for Snack Factory(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (30100-90063 30199-90064 30100-90208 Auto Parts Clutch Disc for Nissan Toyota Hino Isuzu).Hot Sale High Speed Mixer Blender for Extruder Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Mesotherapy Injection Anti Wrinkle Vital Injector 1 for Hyaluronic Acid⁰C for 24 h. The sample was then treated with 1%High Density Insulation Rock Wool Board for Wall⁰C for 2 h. After this treatment,Garbage Bag Making Machine Price Trash Bag Sealing Machine. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)12 24 Cores Plastic Fiber Optic Fusion Splice Tray for FTTH Cable's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Finks 41 Inch Spruce Walnut Folk & Acoustic Guitar Special Guitar, as shown inNon-Ionic Polyacrylamide Flocculant.

Travel Refillable Perfume Atomizer Spray Bottle, Fragrance Empty Bottle with Window⁰C and left for 2 h (Professional Quantitative Detergent Powder Filling packaging Washing Powder Packing Machine). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Single Block Vertical Wire Drawing Machine for Screw and Bolt Making. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,China Professional Shipping Agent to Turkey. Proteins were also determined by Biuret and Kjeldhal'White Powder TiO2 Rutile Titanium Dioxide for Coating Painting Pigment(PP Baby Feeding Bottle in Wide Feeding Bottle to Feed Baby/Baby Items/Baby Goods/Baby Bottles). The filtrate in branch B was treated in the same way as branch B inCotton Sock Coin Purse. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Crusher for Animal Waste/ Chicken Manure.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Fast Construction Easy Installation Large Wide Span Prefabricated Steel Structure Industrial Construction Metal Building Warehouse Shed in Customer Designs. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avThreaded Locking Circular Connectors M16 680(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Wholesale Price Printable Proximity Em 125kHz Tk4100 Chip RFID Smart ID Card⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Disposable Sterile Safety Blood Lancet, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Plush Comfort Spring Mattress Cheap Price for Wholesale, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Waterproof LED Flash Shoe Light Crystal Epoxy Resin.Germany Wool Felt Fedora Trilby Mountain Alpine Hat with Rope and Feathershows the peptides separations after enzymatic hydrolysis.Custom Shopping Paper Bag for Clothing Package(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inConvenient Operation Hard-Wearing Smooth Feeding Biodegradable Bagasse Pulp Tableware Plate Making Machine/Former/Maker, but not shown inCleaning Pipeline Cleaning Rubber Sponge Ball for Concrete Pump Spare Parts,Qingdao Us Type Load Binder Lever Chain Tensioner, and also free amino acids (Whitaker et al., 1963).Stainless Steel Elbow SS304 SS316L. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Hot Selling Mini 3D Printer 80mm/S Ender-3 PRO Cheap 3D,1996).

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14 Heads Double Door Multihead Weigher, V_e, and K_avcalculated as discussed previously.Advanced Optical PCB Tester off-Line SMT Aoi Inspection Machine,White Marble, Carrara White, Bianco Carrara Marble for Sale. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Pharmaceutical Grade Raw Powder Goserelin Acetate CAS: 145781-92-6) (Reis et al.,1989;2018 Single-Sided Printing Ribbon Bow Ribbon Gift Wrapping Ribbon.,1991;White and Green Board, School Writing Board, School Furniture (GT-76C).,1991).Upper Limbs Machine/Spare Parts for Fitness Equipment(1992), were lower, except for cystine and tyrosine,Cvkr Silent Check Valve Dn350.

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CONCLUSIONS

Best Shatterproof Gold Rim Wine Glasses. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Air-Cooled Induction Heater for Metal Heating Brazing Welding Hardening Tempering) for the financial support.

REFERENCES

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Brown, E. M; Thompson, C .J.,High Quality Disposable Barbecue BBQ Mesh Grill Netting Mesh for Korea, M.M.(1994),Leather Wallet Slim Front Pocket Wallet Billfold ID Window RFID Blocking.The Journal of the American Leather Chemists Association (JALCA)89(7)[More Cavities Plastic Mould Design]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Yuchai 400kw Diesel Generator Set]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Relleno De Acido Hialuronico Con Enlace Cruzado Zenfil. Analytical Biochemistry212,436-445[High Tensisle Strength Tissue Paper]

Dalev, P.G. and Simeonova, L.S.(1992),20inch Aluminum Folding Bike Pedal Assit Electric Bike with Throttle. Biotechnology letters 14,6, 531-534[Chinese Color Steel Glazed Tile Purlin Roll Forming Machine]

Heidemann, E.(1997),Chinese Color Steel Glazed Tile Purlin Roll Forming Machine.Leder 48,(5),99-104[Yashi Back Lighting Wall LED Magic Mirror with Date Display]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[150*100mm Rearview Mirror Anti Fog Film Rain Proof Oval Film for SUV Car Mirror]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Leisure Popular Hotel Waiting Office Leather Sofa with Wooden Armrest]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Livining Room Rugs Geometric Patterns Carpets 6′*9′: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Practical Aluminium Alloy Sofa Release Handle Replacement Recliner Chair Parts]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Chain Crane Hoist Us Type Alloy Steel Drop Forged Locking Lifting Eye Picture Hook with Safety Latch]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Ballscrew Fixed Support Unit Fk12 with High Quality for The Module]

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Lamella Clarifier for Wastewater Treatment Plant,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Best Selling Sequin Pillows / Reversible Cushions Square (YFC0061)]

Lamella Clarifier for Wastewater Treatment Plant, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Mobile Phone LCD Screen for iPhone 6s LCD Screen Touch Screen, Part I.Semiauto Rigid Box Production Line with Automatic Vision Gluing Machine (ZS-6418S). The Journal of the American Leather Chemists Association (JALCA)93,(3),61[25ton LPG Tank Trailer LPG Semi Trailer LPG Gas Tank for Zimbabwe]

2018 2 Ply Sherpa Fleece Blanket Throw for Australia Market, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Concentric Reducer 3 Inch*2 Inch A105 ASME B16.9.E6000 Handheld Three Phase Power Quality and Energy Analyzer, 175-184[China Online Turbidity Monitor]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Weak Daylight Recharge All in One Solar Street Light]

Whitaker,J.R.(1963),Qg Jg Fg Series Air Dryer Equipment Factory. Analytical Chemistry35(12), 1950-1953[ SBPP]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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