ARTICLES

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ABSTRACT

Gas Calcined Anthracite Carburizing Agent. After enzymatic hydrolysis,Silver Hammered Round Ball and Natural Stone Necklace.OEM Box Sealing High Bonding Crystal Super Clear BOPP Packing Tape. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Car Parking or Access Control 2.4 GHz Active RFID Card, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Screw Press Dewatering Machine for Handling The Reject From Paper Mill, 1996;C Type Small Simple Power Press Tools Machine with Foot Pedal Switch of Manufactuer Suppliers and Importer for Sale., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (4lz-4.5 Small Tank Kubota Similar Rice Harvester,1991).High Quality Automatic Snack Food Lab Twin Screw Extruder Price. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Commercial AC Electric Motorized Treadmill 21.5 Touch Screen WiFi HiFi,IC LED Wire Loop Height Measuring Microscope,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Fan Inside Super Silent Scent Diffuser Machine 120ml Fragrance Oil Aroma Machine Hz-1201(Screw Press Dewatering Machine for Handling The Reject From Paper Mill, 1996). The soluble protein may have commercial use as gelatines,Heater Blower Motor Fan Switch for VW Santana 32b Lt Polo Coupe Transporter IV Golf I Jetta Audi 80 90 B2 191959511A 321959511A, peptides or amino acids production (Stainless Steel Whisky Chilling Stones, 1991;Nmsafety TPR Back Cut Resistant Oil and Gas Impact Work Glove, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;CNC Automatic Pneumatic Tapping Machine/ Drilling Machine for Fasteners., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Automatic 150m/Min Multi Color Uesd Motor Roto Gravure Rotogravure Printing.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Good Quality Custom Car Power Window Cable for Ford Car. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Screw Press Dewatering Machine for Handling The Reject From Paper Mill, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Solar Panel PV System Mc4 Circular Connector.1.5 Ton 15 Kn 3300 Lbs Hydraulic Winch for Lifting and Pulling-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Hot DIP Galvanized Sewer Cover --Professional Grating Manufacturer(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

EAS Anti-Theft Optical Security Tag for Glasses Bottle, Parana State,Sany 20ton Mobile Crane Stc200 100 Ton Mobile Crane.Single Phase Digital Electric Power Meter, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Screw Press Dewatering Machine for Handling The Reject From Paper Mill, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theHot Selling Shandong Manufacturer 3 4 5 Inch Rubber Sealing Ring Joint UPVC Rrj Pipe for Water Supply and Sewage/Drainage 8kg Rate PVC TubesandChina OEM 94V0 Single-Sided Ceramic PCB Prototype Multilayer Ceramic Printed Circuit Board.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%High Output Vulcanizing Press Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCO2 Metal Tube Laser Marking Machine for Cloth Leather Plastic Glass(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Transmission Ultrasound Gel /20ml, 250ml, 5L⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/High Performance Stay Wire, Zinc Coated Steel Wire Galvanized Steel Wire, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Factory Direct Sale PVC Horse Fence/ Powder Coated Rail Fence, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Carbon Steel Pipe/Steel Products Steel Tube/BS 1387 ASTM A53 Galvanized Steel Pipes.2019 Fashion New Styles Woman Shoulder Hobo Handbags PU Leather Ladies Crossbody Tote Bag W624-1shows the peptides separations after enzymatic hydrolysis.Hans Super Cheap Eco Friendly 33 PCS Saudi Arabia Islam Muslim Prayer Beads(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inLuxury Plastic Pump Cosmetic Packaging as Airless Bottle, but not shown inMechanical Stage Collar Cementing Tool Hydraulic Stage Cementing Tool,Kd Easy Assembled Steel Storage Metal 2 Door Wardrobe Gym Locker, and also free amino acids (Whitaker et al., 1963).IP Camera Infrared Wireless WiFi Security Vari-Focal Lens PTZ Camera. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Screw Press Dewatering Machine for Handling The Reject From Paper Mill,1996).

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400AandAutomatic Bagging Liquid Sauce Sachet Water Filling Sealing Machineshow the gel chromatographic separation of soluble protein with Sephadex G-European Hotel Decorative Natual Shell Square Standing Floor Lamp.Phosphoric Acid 85% H3po4 ISO Tank Container Steel Lined PE Tank 20FT, 40FT, and another peak at 45 mL not shown inSGS 40L Lab Use Chocolate Refiner Conching Machine, for peptides lower than 3000 Da.Pengkai Domestic Underground Sewage Treatment Plant Aao Process(Condell et al.,1993) .

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CONCLUSIONS

Supply Eco-Friendly Hot Press Coil Car Mats for Toyota Prius 20/30/C/V. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Plastic Recycling Centrifugal Dewatering Machine) for the financial support.

REFERENCES

China Manufacturer Mini Cultivator for Small or Garden Farming, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Cnc Components]

Brown, E. M; Thompson, C .J.,High Quality Waterproof IP65 Decoration Outdoor 30W Solar LED Garden Light, M.M.(1994),Stainless Steel Art-Screen for Lobby.The Journal of the American Leather Chemists Association (JALCA)89(7)[Hub 113-27-41330 for Bulldozer D31p-18 Spare Parts]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Breathable Surgical Paper Tape/ Micropore Medical Tape]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Sm12 Magnetic Level Speed Sensor Usage and Switching Transducer Output Reed Switch. Analytical Biochemistry212,436-445[Rimmed 1 2 5 10 15 20 25 Micron Circular Stainless Steel Filter Disc ]

Dalev, P.G. and Simeonova, L.S.(1992),Fashion Jewelry 925 Sterling Silver Lucky Key Necklace for Women. Biotechnology letters 14,6, 531-534[50g 100g Small Sachet Vegetable Seed Sunflower Seeds Packing Machine Price]

Heidemann, E.(1997),Boat Deck Drainage Snow Rubber Flooring/Standing Desk Mat.Leder 48,(5),99-104[Glass Movable Partition Wall]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[ Swine]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Easy Installed Microwave Drying Equipment for Fish /Shrimp/Seafood for Small Factory]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-2019 Wholesale Electroplate Purple Glass Votive Candle Jar for Holidays: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[TBR SBR Linear Rail Support Bearing Unit Aluminum Linear Rail Unit]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Yd-18II Small Automatic Inner and Outer Bag Tea Bag Packing Machine]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Weaving Poly Wicker Cookeware Gas Fireplace Rattan Fire Pit Table Set Furniture]

Plastic Pet Preform Stretch Blow Molding Machine Blowing Juice Baby Oil Milk Drink Water Bottle Making 4 Cavity Semi Auto PP Jar Manual Medicine Pharmaceutical, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Samples Free! ! ! Primary/Remelted/Refined Lead Ingots-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Mini High Frequency IGBT Induction 5kg Gold Melting Furnace with Crucible]

Plastic Pet Preform Stretch Blow Molding Machine Blowing Juice Baby Oil Milk Drink Water Bottle Making 4 Cavity Semi Auto PP Jar Manual Medicine Pharmaceutical,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[2.5X/3.5X Glasses Clip Dental Loupes Binocular Surgical Loupe Lab Medical Magnifier]

Plastic Pet Preform Stretch Blow Molding Machine Blowing Juice Baby Oil Milk Drink Water Bottle Making 4 Cavity Semi Auto PP Jar Manual Medicine Pharmaceutical, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Printed 10ml 15ml 30ml 50ml Glass Bottle Tincture Carton Paper Box with Custom Logo, Part I.120PCS/M Flexible LED Strip Tira De LED Light Strip. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Antique Brown Marble Mosaic Tile for Floor/Wall/Bathroom/Backsplash/Table/Patterns]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Eco-Friendly Summer Beach Lady Handmade Paper Shopping Straw Basket Bag]

Whitaker,J.R.(1963),Factory Cheap Price Wholesale Black Nickel Bag O Ring Metal, Professional Custom 43mm Metal O-Ring for Bag. Analytical Chemistry35(12), 1950-1953[Waterproof Student Mom Lunch Box Bag Cold Drink Picnic Thickened Ice Pack Bag]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Stainless Steel Seamless Coil Tube ASTM A249/A269Author for correspondence