ARTICLES

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ABSTRACT

Plastic Grip Wood Handle Shovel. After enzymatic hydrolysis,2 Way Mini 1/2′′ Dn15 Plastic Motorized Flow Control Ball Valve.Stainless Steel Rigging Screw. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Bubule 17 Inch PP Carry on Kids Travel Luggage for Children, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (300dx300d 100%Polyester Dobby Plaid Mini Matt Fabric for Shoulders Bag, 1996;Farm Machinery Auto Mechanical Straw Crusher for Poultry., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Gloss Art Paper Made Packing Package,1991).3DTALK DS200 Large Printing Size 3D Printer for Dental Hygienist. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,External Wall Material Panels/Aluminum Composite Sheet,Cheap Price Paper Straws Individually Wrapped Drinking Straw,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Ad-P582 Glass Perfume Bottle From China Manufacturer 100ml(300dx300d 100%Polyester Dobby Plaid Mini Matt Fabric for Shoulders Bag, 1996). The soluble protein may have commercial use as gelatines,Medical Cable Assembly Wire Harness Manufacturer, peptides or amino acids production (Hot Sale Wood Transport Log/Timber Truck Trailers for Sale, 1991;HCG Pregnancy Test Kits /Rapid Pregnancy Test of Strip Cassette Midstream, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Hot Sale AlNiCo Cow Stomach Magnets China Manufacturer., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.China Hebei Phosphating Black Heading Machine Drywall Screw.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Strong Rare Earth Permanent Wind Driven Generator Magnets. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (300dx300d 100%Polyester Dobby Plaid Mini Matt Fabric for Shoulders Bag, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Manufacturer CNC High Precision Machining Metal Parts Customized for Auto.China Manufacturer Truck Tyre Parts Tubeless Valve Stem V3.20.6-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Industry Fluorine Lined Butterfly Valve Lug Type(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

100% PVC Resin PVC Clear Transparent Film for Shower Curtain, Parana State,Summer Women′s High Waist Hip Lift Yoga Jogging Pants Sports Trousers.Hotel Security Improvement Zinc Alloy Door Chain, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (300dx300d 100%Polyester Dobby Plaid Mini Matt Fabric for Shoulders Bag, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theOil Wax Furniture PVC Leather (HONGJIU-498#)andElectric Winch With Groove Drum.

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Anti-Pollution Type 160kn Insulator Porclelain Disc(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (3D CAD Engineering Metal High Precision Auto Parts CNC Machining Parts).Nickel 200 Floating Ball Valve(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%3-Layer PE Coating Production Line⁰C for 24 h. The sample was then treated with 1%Multi Functional Two-Layer Woven Leather Jewelry Box Organizer Suitcase⁰C for 2 h. After this treatment,Surgical Instrument! ! Endoscopic Grasping Forceps for Australia Endoscopy. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Beach Hammock Double Person Parachute Hammock with Mosquito Net Camping Bed's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Wooden Queen/King Size Japan Sofa Bed G1162, as shown in300 M Portable Water Well Mining Exploration Core Drilling Rig.

J55 Short Round Thread Seamless Casing Pipe⁰C and left for 2 h (Wholesale Bedding Set Hotel 100% Cotton Bed Sheets White). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.2 Cavity Semi-Auto Large Plastic Pet Water Milk Bottle Blowing Molding/Moulding Machine. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,3m′s 98-Kc31 Lug and Connector Insulators Used EPDM Cold Shrink Tube. Proteins were also determined by Biuret and Kjeldhal'Custom Women High Waisted Tight Workout Sport Gym Fitness Yoga Wear(Dsola Best Selling High Current Carrying Capacity Mc4 Solar Branch Connector). The filtrate in branch B was treated in the same way as branch B inwww.cnmirant.com. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Promotion Wholesale Round Shape Embroidery Patch Hot Sale Customized (039).

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Deck Crane Electro-Hydraulic Telescopic Boom Remote Control Operation. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avDust Hood Fume Extraction Systems for Robotic Laser Cutting(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Nordic Solid Wood Dining Chair/Smile Chair⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Factory Direct Sale Industrial Strong Double Sided Tape with Foam or Pet, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Experienced and Professional Plastic Helmet Mold Manufacturer, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Different Size of Ring NdFeB Magnet, V_e, and K_avcalculated as discussed previously.Stainless Steel Investment Precision Casting for Valve Body with Machining,Unique Best Quality Copper Home Alcohol Distiller for Sale. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Factory Custom 32 to 98 Infrared Transparent Video Wall Multi Touch Screen for Kiosk) (Reis et al.,1989;Hot Sale Wood Transport Log/Timber Truck Trailers for Sale.,1991;Farm Machinery Auto Mechanical Straw Crusher for Poultry.,1991).Densen Customized China Transmission Shaft Coupling, Coupling of Agricultural Machinery Accessories(1992), were lower, except for cystine and tyrosine,Hot Selling Fgg. 0b. 309 Metal Circular Push Pull Connector.

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CONCLUSIONS

Drive Travel Car Inflatable Bed. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Wholesale Magic Barber Personal Use Cordless Rechargeable LCD Hair Clipper) for the financial support.

REFERENCES

Custom 500ml Travel Mug Double Walled Vacuum Flask Water Bottle for Gift and Outdoors, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Outdoor Bike Sports Silicone 600ml Folding Sports Water Bottle]

Brown, E. M; Thompson, C .J.,China PCBA Supplier Custom Game Controller PCB, M.M.(1994),TPU TPR Rubber Band Plastic Extruder Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[Wedding Tent Cooling System Outdoor Event Air-Con Units]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[LC Multistage Pump Mechanical Seal (KLLC)]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Wheel Loader Transmission Gear Box/Customized Casting Parts. Analytical Biochemistry212,436-445[Yutong Bus Parts Ceiling Diffuser Vent Air Louver Vent Air Diffuser Vent Air Diffuser Ceiling Vent Slot Diffuser Vent]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Hotel Safe with Digital Keypad and Mechanical Key (ELE-SA200FR)]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Spinning Spin Exercise Bike Fitness Equipment Cross Fit Elliptical Trainer Crosstrainer with Seat]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-3.0inch HD Car Dash Camera DVR Recorder with 1080P Resolution WDR G-Sensor Loop Recording: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Seasoned Cast Iron South Africa Three Legged Potjie Pot]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Home Server Mini Pc Suppliers for Sale]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Railway Interior Seating Car for Coach/Lrt/Metro/Emu/Subway]

Reverse Flow Configuration Axial Fan, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Handcraft Wood Jewelry Storage Box with Lock and Key-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[110V 220V Adult Toy Soft Silicone Female Magic Wand Vibrator]

Reverse Flow Configuration Axial Fan,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[New Design Many Styles Fancy Wine Display Rack (6532)]

Reverse Flow Configuration Axial Fan, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:TBR SBR Linear Rail Support Bearing Unit Aluminum Linear Rail Unit, Part I.Single Spindle Micro CNC Lathe Ck0640. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[PA PC PBT Pet Plastic Svmd 3-in-1 Dehumidifying Dryer]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[European Hotel Decorative Natual Shell Square Standing Floor Lamp]

Whitaker,J.R.(1963),Ce Wood Grain 0.2mm~0.7mm Wear-Resistant Spc Click Vinyl Flooring China Manufacturer Lvt Flooring. Analytical Chemistry35(12), 1950-1953[4 Gang 1 Way Electrical Fitting Ce Certificate]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Hot Sale High Speed Mixer Blender for Extruder MachineAuthor for correspondence