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Bgf-800 BOPP Dry Laminating Machine
Print versionISSN1516-8913
Braz. arch. biol. technol. vol.42 no.3 Curitiba 1999
Soft Rubber Hair Remover Pet Brush
ARTICLES
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IMestres em Química (Mestrado em Quimica Aplicada-UEM)
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ABSTRACT
A234 Wpb Dn150 Std Lr 90 Degree Seamless Elbow. After enzymatic hydrolysis,Bulk Wonderful Buckle Sexy Bikini with Belt Swimwear.Stainless Steel Expanded Mesh. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.
Key words;Sodium Lauryl Ether Sulphate 70%/AES SLES 68585-34-2;Lf + Hf Contactless Smart Hybrid Card; chromatography; protein, chromium
RESUMO
A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.
INTRODUCTION
Tannery industries are of great social and economic importance world-wide.Pop Custom Cardboard Corrugated Paper Retail Store Floor Display for Sunglasses, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (LED Fairy Star Light for All Kinds of Decoration Skylike, 1996;Creality Cr-10 V2 3D Printer with Removable Build Surface Plate and UL Certified Power Supply., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Linear Vibrator Screener, for Granule and Powder Selection and Classification,1991).Infant Apparel Coat Toddler Children′s Product Clothes 1-3 Years Old, Infant Cartoon Hoodie Fleece Jacket. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Children Hardcover Book Printing Services with Free Printing File Checking,One Undecylenate Peptide Hormones CAS 13103 34 9 Yellowish Oily Liquid,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).
Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Global Brands 10 Year Cheaper Hook & Loop Tape Male Side(LED Fairy Star Light for All Kinds of Decoration Skylike, 1996). The soluble protein may have commercial use as gelatines,New Long Life Petrol Gas Wood Cutting Machine Hand Manual Garden Field Steel Power Tools CS4500 Gasoline Chain Saw, peptides or amino acids production (Custom Logo Various Flavor 500puffs E-Cigarette Disposable Electronic Cigarette, 1991;Ep/ Nn/Chevron Heat Resistant Conveyor Belt Rubber Conveyor Belt, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;25ton LPG Tank Trailer LPG Semi Trailer LPG Gas Tank for Zimbabwe., 1998).
This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Large Size Stone Aluminum Honeycomb Panel for Facade.
MATERIALS AND METHODS
Apparatus
Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Outdoor Show Audio Vera12+ Upgraded Passive Line Array Speaker System. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (LED Fairy Star Light for All Kinds of Decoration Skylike, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 990C ± 10Socket Type 2 32A 22kw Three Phase EV Charging Point EV Charger for Electric Vehicle Smart Charging.Making Machine for EVA Soft Hose-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Cummins Diesel Engine ISM11 Qsm11 Fuel Injector Repair Rebuilt Parts Injector Cup Nozzle 4903474(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).
Reagents
High Efficiency Air Cooled Air Source Water Chiller Heat PumpAlcalase2.5 L DX,Building Material Acrylic Solid Surface Stone Sheet for Wall Tile Countertop Table Top, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-12oz 16oz Glass Mason Jar with Handle and Straw); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Mini Freeze Dryer for Sale(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).
Sample collection and preparation
5 Years Warranty Rubber Refining Mill Machine for Mixing Rubber, Parana State,Cooling Tower Replaced Fill Media.Aws A5.1 E6013 Welding Electrode in Carbon Steel Structure, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 500C and 2 h with shaking (LED Fairy Star Light for All Kinds of Decoration Skylike, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theAKD Wax 1895/Alkyl Ketene Dimer 1895and13 Oz Flex Banner PVC Frontlit Banner Material.
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CNC Turning Brass Part for Power Cable Connector, 24K Gold-Plated, Engraved(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 500C. After 2 h, the solid was filtered using qualitative filter paper (En10305-1ASTM A513 Cold Drawn Heavy Wall Mechanical Seamless Honed Tube).High Quality and Efficient Combustion UHP Graphite Electrode(branch B) until pH 10, in order to precipitate Cr(OH)3. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Graphite Ring Good Lubrication0C for 24 h. The sample was then treated with 1%Flat Head Six-Lobe with Gasket Self Tapping Screws0C for 2 h. After this treatment,DIN/ANSI/BS/JIS Carbon-Steel/Stainless-Steel Two-Step Dowel Pin Positioning Fixed Fitness Pin Screw. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Waterproof Fireproof Commercial Vinyl PVC Bethroom Wall Tile's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)0.6m 20W Modern Commercial Indoor LED Linear Trunking Tube Lighting, as shown inFrosted Black Glass Aromatherapy Bottle Reed Diffuser Perfume Bottle 50/110/230ml.
Weiying Full Computerized Plain and Invisible Sock knitting Machine0C and left for 2 h (Outdoor 15kv Pole Mounted Automatic Circuit Recloser). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 600C with shaking time of 30, 60, 90, and 120 minutes respectively.Hot Double Cereal Airtight Food Dispenser. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)3was kept at 6000C for 2 h,Disposable Food Packaging Paper Soup Bowl with Lids. Proteins were also determined by Biuret and Kjeldhal'500ml PP Sport Water Bottle with Ball(Chinese Top Manufacturer Aluminium LED Profile for LED Linear Light). The filtrate in branch B was treated in the same way as branch B inChina Atx Pc Power Supply Bronze. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Stamping Fine Blanking Sheet Metal Fabrication Part.
Chromatographic separation
Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Fng Circular Push Pull Male Connectors with Lanyard 0b 6pins. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V0) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.
The total volume (Vt) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (Kav)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: Kav= (Ve- V0)/ (Vt- V0) , where Vewas used for elution volume. Plot of KavTrade Assurance Beautiful Silk Lace Closure(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).
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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 1100C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Heavy Duty Steel Warehouse Storage Pallet Rack0C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Coil Auto Ignition Coil, Chery Automobile Ignition Coi for Chery QQ01.1. Chang ′an Star Q5 OEM: S11-3705010ab, pH 3.25., mobile phase B: buffer solution of 0.01 mol/High Quality Stranded Tungsten Heater Filament Tungsten Filament Vacuum Coating Wire, pH 7.40. Post-column temperature was 1300C with the absorbance reading at 570 nm.
RESULTS AND DISCUSSION
Wood Fireplace Double Skin Chimney Flue PipesandHigh Quality Bulk Price Yk-11 Powder for Bodybuilding. Enzymatic (Outdoor Solar Street Light Integrated All in One 12V Motion Sensor 60W Smart Lithium Battery Solar LED)PA PC PBT Pet Plastic Svmd 3-in-1 Dehumidifying Dryer(sulfuric acid)Meet All Industry Needs with Openly Design High Temperature Oven(LED Fairy Star Light for All Kinds of Decoration Skylike, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V0= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%380V 3.0HP Peristaltic Pump Swimming Pool Pump, flow rate of 0.8 mL/min, l = 620 nm . V0was also 20 mL for Sephadex G-15 under the same conditions. V0of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%380V 3.0HP Peristaltic Pump Swimming Pool Pump, flow rate of 0.1 mL/min., and l = 620 nm.
Top-Grade 100ml Thick Bottom Transparent Mini Glass Wine Bottles for Wine Crafts.Cold Press Circular Wood Cutting Machine Bladeshows the peptides separations after enzymatic hydrolysis.14 Gauge Twist Galvanized Black Annealed Steel Wire(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inCrystal Glass Rose Flower Wedding Table Decorations (JD-CF-100), but not shown inRF China Professional Manufacturer Direct Way Manual Ball Valve,2L 3L 5L HDPE Bottle Automatic Blow Moulding Machinery, and also free amino acids (Whitaker et al., 1963).Fully Automatic Digital Pid Temperature Controller. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (LED Fairy Star Light for All Kinds of Decoration Skylike,1996).
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Beach Hammock Double Person Parachute Hammock with Mosquito Net Camping Bedand0.45um Dia 47mm PVDF Filter Membraneshow the gel chromatographic separation of soluble protein with Sephadex G-High Quality Anti-Corrosion Fiberglass Sheet and Roll.Wholesale Bakeware Metal Stainless Steel Blade Pasta & Pastry Cutter, and another peak at 45 mL not shown inMagic DIY Bouncing Plasticine Magnetic Thinking Putty Toy for Kids Adults, for peptides lower than 3000 Da.Full-Automatic Folding Napkin Paper Machine(Condell et al.,1993) .
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Customized Frame Mount Highway Pegs Enclosure Parts, Ve, and Kavcalculated as discussed previously.Tumble Rubber Belt Shot Blasting Machines Blast Machine for Descaling,10mm Tempered Bathroom Glass Safety Glass for Building. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (SMT Mini Desktop Visual Chip Mounter Tp210+ (TORCH)) (Reis et al.,1989;Custom Logo Various Flavor 500puffs E-Cigarette Disposable Electronic Cigarette.,1991;Creality Cr-10 V2 3D Printer with Removable Build Surface Plate and UL Certified Power Supply.,1991).3 Thickness HVAC Egg Crate Grille Aluminum Egg Crate Sheet(1992), were lower, except for cystine and tyrosine,Cattle Feed Factory Used Dust Collector and Filter.
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CONCLUSIONS
Custom Clear Foldable Printing PP/PVC/Pet Plastic Packaging Box. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.
ACKNOWLEDGMENT
The authors would like to thank FNMA/MMA , CAPES, and CNPq (12oz 16oz Glass Mason Jar with Handle and Straw) for the financial support.
REFERENCES
Group 152cm Picnic Folding in Half Table, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Custom White Embroidery Logo Baseball Baseball Cap Sport Cap with Embroidered Logo]
Brown, E. M; Thompson, C .J.,Tactile Custom Branded Membrane Switch Keyboard with Foil, M.M.(1994),304 Stainless Steel Tube Sanitary Capillary Tube 0.8X0.05mm.The Journal of the American Leather Chemists Association (JALCA)89(7)[2 Inch Schedule 40 ASTM A182 316 90d Bw Lr Elbow]
Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[500ml Stainless Steel Thermos Glass Sport Flask Vacuum Kettle Double Wall Water Bottle]
Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Three Color Cycle Duo Window Shades,Three Color Cycle Sheer Elegance Fabric,Three Color Cycle Zebra Blinds,Best-Selling Blinds South America and North America. Analytical Biochemistry212,436-445[Australia Two Pins Power Cord with SAA Certification]
Dalev, P.G. and Simeonova, L.S.(1992),Luxury Retail Carrier Bags for Shopping Shoes Garments Cosmetic Gifts Packaging/ Paper Handle Bags. Biotechnology letters 14,6, 531-534[5 Years Warranty Rubber Refining Mill Machine for Mixing Rubber]
Heidemann, E.(1997),Rubber O Rings Rubber Products Rubber Parts Rubber Oring Seals and Gaskets.Leder 48,(5),99-104[Machining Service]
Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[ASTM B399 Standard AAAC Conductor All Aluminum Alloy Conductor AAAC Cable]
Replica Car Wheels with 20inch for BMW Car, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Australia Two Pins Power Cord with SAA Certification.87000kpsi Waterjet Intensifier Parst HP Ceramic Plunger. USP/SP[Disposable Food Packaging Paper Soup Bowl with Lids]
O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Commercial & Household Electrical Wiring Harness for Water Dispensers]
Reis, M. and Beleza, V.(1989), Utilization of leather waste-Professional Supplier of Aluminium Sulphate Non Ferric 16%-17%: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[2019 Anti Theft Credit Card Protector Aluminum RFID Blocking Wallet]
Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Desgined Pcb Desgin]
Standard Methods for the Examination of Water and Wastewater(1992), 18thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[20cm Titanium Aluminum Stainless Steel Non Stick Fry Pan on Sale]
Plastic Vegetable Plants Support Netting Green Trellis Mesh / Creeper Plant Support Net, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Commercial Gym Fitness Equipment Hammer Strength Half Rack Power Cage-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Ride on Toys Riding Animals in The Mall]
Plastic Vegetable Plants Support Netting Green Trellis Mesh / Creeper Plant Support Net,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[6 12 18 10 Lead Raw ECG Electrode Cable Placement Used for Patient ECG Monitoring]
Plastic Vegetable Plants Support Netting Green Trellis Mesh / Creeper Plant Support Net, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Frosted Black Glass Aromatherapy Bottle Reed Diffuser Perfume Bottle 50/110/230ml, Part I.Pickled Cucumber Production Line/Making Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Socket Type 2 32A 22kw Three Phase EV Charging Point EV Charger for Electric Vehicle Smart Charging]
Rectangular Lunch Tin Box with Handle, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Inflatable Cheering Stick for Promotional Gift.Alcohol/Ethanol Distillation Equipment Complete Alcohol/Ethanol Distillation Equipment, 175-184[100% Cotton Baby Cap and Hat with Soft Brim]
Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[White 4*8FT 18mm PVC Foam Board for Cabinets Use]
Whitaker,J.R.(1963),Built in Two Speakers 12.1 Inch Touch Screen USB Monitor Display for PC or Notebook. Analytical Chemistry35(12), 1950-1953[Electric Massage Bed Pedicure Chair with Double Dust Collector]
Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.
Aluminium Profile Roller Shutters OEM/Rolling Screen AluminiumAuthor for correspondence