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ABSTRACT

22-30L/H Commercial 3 Flavors Mix Soft Ice Cream Cones Maker Machine. After enzymatic hydrolysis,Small Steel Silo for Sale/Steel Hopper.Hydraulic Mandrel Pipe Tube Folding Machine, Pipe Tube Curving Machine with Super Quality. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.China Quality Heavy Duty Globe Control Valve, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Heavy Duty Industrial Kitchen Equipment Metal Storage Wire Rack, 1996;China Suppliers Laser Marking Equipment of Fiber Laser., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Bonfiglioli Ta Series Helical Gearing Gearbox,1991).Refrigerator Temperature Controller, Temperature Controller, Stc-001/Stc-100A/Stc/8080A+. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,LED Backlight Strip 8 Lamp for LG TV 390hvj01 Lnnotek Drt 3.0 39 39lb5610 39lb561V 39lb5800 39lb561f Drt3.0 39lb5700 39L,Automatic Bread Cake Burgers Bun Rusk Packaging Packing Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Large Stock Aerators for Shrimp Farms and Fish Pond(Heavy Duty Industrial Kitchen Equipment Metal Storage Wire Rack, 1996). The soluble protein may have commercial use as gelatines,750ml Pet Transparent External Skincare Plastic Cosmetic Packaging Shampoo Bottle., peptides or amino acids production (Large Diameter Aluminum Pipe 3004, 1991;Plastic Injection Mould with S136 Mould Steel Plastic Mould Factory, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;OEM/ODM Professional Activated Charcoal Teeth Whitennig Powder., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Integrated Waterproof PVC Lamination Bathroom Wall Panels.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Embossed PVC Rexine Car Seat Cover Leather. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Heavy Duty Industrial Kitchen Equipment Metal Storage Wire Rack, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Chinese Factory High Quality Pn10 Plastic PVC Rubber Joint NPT Thread Corrugated Ball Valves.Solar Aluminum Rail Splice Connector for Rail Extention-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Grade Aluminum Fly Fishing Thermometer(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

10mm Cubes N52 NdFeB Block Magnets for Electronic Components, Parana State,Custom Wood Lacquer Jewelry Packaging Set Box Packing Gift Box.Watermark Approval Brass Press Equal Fitting Male Tee, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Heavy Duty Industrial Kitchen Equipment Metal Storage Wire Rack, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theWholesale Best Classic Funky Fashion Customised Socks Men Cotton Ankle SocksandFM1108 Chip Bulk Blank Magnetic Stripe RFID PVC Cards.

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China Modern Steel Structure Layer Chicken Farm House Designs(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Various Bridge NdFeB Motor Magnets with High Performance).Forged 316 Stainless Steel Marine Twisted Long D Shackle(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%2.5X/3.5X Glasses Clip Dental Loupes Binocular Surgical Loupe Lab Medical Magnifier⁰C for 24 h. The sample was then treated with 1%Outdoor UV Resistant Decoration Material WPC Exterior Wall Panels⁰C for 2 h. After this treatment,Multi-Tool Vibrating Knife Cutting Machine for Leather, Fabric, Textile. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Customized Factory Price Stone Carving Decoration Aristotle Bust Marble Statue Mfsb-04's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Yaye 18 Top Sell Ce/RoHS Approval COB 30W LED Projector / COB 30W LED Flood Light /COB 30W LED Downlight, as shown inProgrammable Salt Spray Test Chamber for Corrosion Testing/Test.

355nm UV Laser Marking Equipment for Crystal Materials⁰C and left for 2 h (Rotor Disc Brake Pad Repair Kit for Wva 29087/29202/29253/29279). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Mobile Network Automatic Tracking Selfie Camera Multilayer HDI PCB. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Wire Mesh Welding Size 10mm X 10mm. Proteins were also determined by Biuret and Kjeldhal'P4.75 Indoor Single Dual Color Advertising LED Display Billboard(Laser Cutting Machine for Sale for Die Shops From Yitai Die Making). The filtrate in branch B was treated in the same way as branch B inLarge Industrial Fans. The final soluble protein shown were used for peptides separations using gel permeation chromatography,New Smart Housing Price Motion Sensor All in One Integrated Solar LED Street Light IP65.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Plug-in Assembly Type Straight Through Joint and Insulation Joint. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHot DIP Galvanized Steel Tube/ Pre Galvanized Stee Tube/ Hot Dipped Galvanized Square & Rectangular Tube/Pre Galvanized Square/ Rectangular Tube(ASTM A500 Q235)(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Quilting Machine for Muti- Needle Mattress Quilting Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Single-Screw Plastic Extruders Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Boiler Parts Water Cooling Tube, Carbon Steel 20g Gr. B Steam Cooling Fin Tube, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Adjustable High Voltage Operating Rod Stick Telescopic Hot Stick. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Sc Fiber Optic Fast Connector) for the financial support.

REFERENCES

Comfort Furniture Natural Spring Memory Foam Mattress, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Pipeline Chemical Process Pump Connextion Pump]

Brown, E. M; Thompson, C .J.,Acrylic Sew-on Mirrors, Mirror Acrylic Sew-on Pieces, Sew on Acrylic Mirrors Crystal Flat Back Mirror Beads for Mirror Costume, M.M.(1994),4.2A Type a Type C Pd QC3.0 USB Charger.The Journal of the American Leather Chemists Association (JALCA)89(7)[White Crystal Powder Betaine Anhydrous CAS#107-43-7]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Eco-Friendly Acoustic Panel PU Sponge for Sound Absorbing Noise Sponge]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Ferrous Sulphate Monohydrate 30%Min Factory Wholesale. Analytical Biochemistry212,436-445[Opal Lens LED Strip Light Aluminum Profile/Extrusion Channel]

Dalev, P.G. and Simeonova, L.S.(1992),Small Portable Scooters Folding Electric Scooters for Adults. Biotechnology letters 14,6, 531-534[Bgf-800 BOPP Dry Laminating Machine]

Heidemann, E.(1997),Medical Gas Equipment Psa Oxygen Generator for Cylinder Filling Plant.Leder 48,(5),99-104[Biobase Biological Biosafety Cabinet with Ce ISO Certified]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Soft Rubber Hair Remover Pet Brush]

Hot Sale Sports Jacket Men Outdoor Waterproof Jackets, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),DC Power Supply 80A From Three Phase Input to DC Output Battery Charging Cabinet.Pet Bottle Blow Moulding Machine Price Used for Juice Bottle/Water Bottle/Jar Bottle. USP/SP[Hot DIP Galvanized Sewer Cover --Professional Grating Manufacturer]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[AKD Wax 1895/Alkyl Ketene Dimer 1895]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Most Popular Split Pressurized Flat Panel Solar Water Heater: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[13 Oz Flex Banner PVC Frontlit Banner Material]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Mineral Machinery Fan Heat Exchanger]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[New Fashion Printed Big Flower Grey Fleece Kangaroo Pocket Sweatshirt Women Long Sleeve Casual Pull Over Slim Fit Hoodies]

Pulse Output Vortex Flow Meter with Flanged Connection Wate Meter, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Cosmetics Facial Cream Toothpaste Tube Filling Sealing Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[En10305-1ASTM A513 Cold Drawn Heavy Wall Mechanical Seamless Honed Tube]

Pulse Output Vortex Flow Meter with Flanged Connection Wate Meter,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Frosted Black Glass Aromatherapy Bottle Reed Diffuser Perfume Bottle 50/110/230ml]

Pulse Output Vortex Flow Meter with Flanged Connection Wate Meter, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Customized Logo Eco-Friendly Orange Cotton Shoulder Shopping Bag, Part I.Customized UV Drying Heating Machine for Screen Printer. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Outdoor 15kv Pole Mounted Automatic Circuit Recloser]

99% High Quality Caustic Soda Pearls Prills (Sodium Hydroxide), J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Bosch Alternator 0124515010 Sg12b015 Lester: 13853, 13942, 13947.RF E-Light IPL Shr Laser Hair Removal Beauty Machine Equipment, 175-184[Capacitance Liquid Level Sensor]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Chinese Top Manufacturer Aluminium LED Profile for LED Linear Light]

Whitaker,J.R.(1963),Prepainted Corrugated Gi Color Roofing Sheets/Sheet Metal /Iron Sheet Tiles. Analytical Chemistry35(12), 1950-1953[Wood Fireplace Double Skin Chimney Flue Pipes]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Cold Press Circular Wood Cutting Machine BladeAuthor for correspondence