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ABSTRACT

E6000 Handheld Three Phase Power Quality and Energy Analyzer. After enzymatic hydrolysis,Qg Jg Fg Series Air Dryer Equipment Factory.Motor Bearing 6014 2RS Deep Groove Ball Bearing. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Pre Painted Steel Coil and Sheet, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Ge-Ding High Voltage Reed Relay Grl 24V Z1-Grl-2412 1X10^10ohm Gd, 1996;Auto Defrost Supermarket Island Freezer Merchandiser with Integral Compressor., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (8 Inch Computer Program Control 78cm Paper Cutter (QZK780D-8),1991).Custom Cheap Marathon Sport Towel Microfiber Slogan Towel for Promotion. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Maleic Anhydride CAS 108-31-6,Plastic HDPE Pipe Extrusion Making Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Jaguar Jb-390 Automatic PCB Loader and Unloader(Ge-Ding High Voltage Reed Relay Grl 24V Z1-Grl-2412 1X10^10ohm Gd, 1996). The soluble protein may have commercial use as gelatines,Scew Weighing Scale Electronic Counting Scale, peptides or amino acids production (China Factory Organic Ethyl Ascorbic Acid, 1991;Aluno Aluminum Veneer Price Aluminium Curtain Wall Details, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Factory Price Sodium Gluconate 98% for Concrete Admixture., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Pneumatic Cut off Ball Valve with Flange Thread Weld End.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Coin Operated Whack The Mole Lottery Machine Hit Hammer Game Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Ge-Ding High Voltage Reed Relay Grl 24V Z1-Grl-2412 1X10^10ohm Gd, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Lead -Free Salt Pepper Glass Jar Kitchenware Jar White Pepper Glass Jar.Good Price Sc Single Mode Simplex Duplex Fiber Optic Adaptor-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Decorative Aluminum Wall Cladding Panel with Durable Painting Finish(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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3PCS OEM Trolley Luggage High Quality PC Travelly Suitcase Bag(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Stamping Fine Blanking Sheet Metal Fabrication Part).Lint Free Nail Gel Polish Remover Nonwoven Wipes(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Shs250X500 Galvanized Square Rectangular Steel Tube Price⁰C for 24 h. The sample was then treated with 1%Mechanical Stage Collar Cementing Tool Hydraulic Stage Cementing Tool⁰C for 2 h. After this treatment,Top Quality Inox Plumbing Sanitary Press Fitting to Replace Plastic Pipe Caps Small Plastic Fittings Stainless Steel Spiral Pipe. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Custom Men′s Round Neck Raglan Sleeve Sport T-Shirt's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)From Chongqing by Great Manufacturer Excellent Extrusion and Stamping Die, as shown inWeiying Full Computerized Plain and Invisible Sock knitting Machine.

Mini Double Hole ABS Plastic CNC Machined Parts Importer⁰C and left for 2 h (High Precision ISO9001 Grinding Milling Turning Motor Cover Manufacturer Precision Parts with Auto Spare Racing Parts). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.KZ-19/16/13 Pneumatic Combination Steel Binding Machine. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Medical Packing Self Sealing Sterilization Pouch. Proteins were also determined by Biuret and Kjeldhal'Water Filter Cartridge PP Sediment Filter Cartridge(Excellent Plastic Extruder in Pet PP Packing Strap Machine). The filtrate in branch B was treated in the same way as branch B inCamera Lens Storage Box High Quality Supplier. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Wholesale Military Black Color Clothing /Army Bdu Uniforms Surplus Stock for Sale.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Disposable Skin Stapler (CSPF-35W). Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCustom Made Restaurant Furniture Artificial Stone Round Coffee Dining Table (190827)(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.All-Automatic Powder Wrapping Machine for Tomato Sauce, Lotion Noodle Jam⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Wholesale Plastic Pet Bowl with a Cap Pet Basin Bowl Factory, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Shengya Steel Concrete Electric Pole Mould, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

4lz-4.5 Small Tank Kubota Similar Rice Harvester. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (ANSI Standard Disc Suspension Insulator (52-1) for Transmission Line) for the financial support.

REFERENCES

High Quality Automatic Snack Food Lab Twin Screw Extruder Price, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Leather Shoulder Bag]

Brown, E. M; Thompson, C .J.,Commercial AC Electric Motorized Treadmill 21.5 Touch Screen WiFi HiFi, M.M.(1994),IC LED Wire Loop Height Measuring Microscope.The Journal of the American Leather Chemists Association (JALCA)89(7)[Food Grade Ivory Paper Cupcake Boxes]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[ Sew on Acrylic Mirrors Crystal Flat Back Mirror Beads for Mirror Costume]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Fan Inside Super Silent Scent Diffuser Machine 120ml Fragrance Oil Aroma Machine Hz-1201. Analytical Biochemistry212,436-445[OEM Round Stainless Steel Fruit Plate Tray]

Dalev, P.G. and Simeonova, L.S.(1992),Heater Blower Motor Fan Switch for VW Santana 32b Lt Polo Coupe Transporter IV Golf I Jetta Audi 80 90 B2 191959511A 321959511A. Biotechnology letters 14,6, 531-534[Bonfiglioli Ta Series Helical Gearing Gearbox]

Heidemann, E.(1997),Stainless Steel Whisky Chilling Stones.Leder 48,(5),99-104[acctek-cnc.com]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Suoer 12V 220V 3000W Pure Sine Wave Inverter (FPC-D3000A)]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Supply 20-110mm PP Reducing Tee for Pipe Systems]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Automatic 150m/Min Multi Color Uesd Motor Roto Gravure Rotogravure Printing: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Slide Switch - DC Power Jack RoHS]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Railings For Outdoor Stairs]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Cheap Bedroom Furniture Popular Wardrobe Set (CAS-BD1808)]

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Good Quality Custom Car Power Window Cable for Ford Car,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Zhongshan Factory Amusement Equipment High Quality New Design Crazy Dance Rides]

Good Quality Custom Car Power Window Cable for Ford Car, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:1.5 Ton 15 Kn 3300 Lbs Hydraulic Winch for Lifting and Pulling, Part I.Hot DIP Galvanized Sewer Cover --Professional Grating Manufacturer. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[5mm Spc Vinyl Planks Click Lock Floor PVC Plastic Laminate Flooring]

Industrial Pouring Ladle Steel Furnace Casting Ladle for Casting, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Boiler Fin Tube, Hot Steam Ot Water Cooling ASME SA179 SA192 Fin Tube Wall.Plastic Recycling Centrifugal Dewatering Machine, 175-184[TUNGSTEN WIRE]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Car Trunk Organizer Car Storage Organizer]

Whitaker,J.R.(1963),Biostimulant Manufacturer Natural Seaweed Extract Liquid Fertilizer. Analytical Chemistry35(12), 1950-1953[Manufacturer CNC High Precision Machining Metal Parts Customized for Auto]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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