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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-360 Degree Reciprocating Car Wash Machine. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Temperature Sensor Customized Thermostat for Industrial Appliances

ABSTRACT

Custom Die Aluminum Castings. After enzymatic hydrolysis,High Quality Meat Pie Making Machine with Different Mould Shape.Gear Operated Flanged High Performance Butterfly Valve. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;China Supplier Sinotruk HOWO 6X4 Trailer Head Tractor Truck;Stainless Steel Autoclave UV Sterilizer for Salon Yj-UV3; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Wholesale Universal Garden Smart Flower Pot, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Reflective Tape for Vehicle Reflective Markings (tape-02), 1996;White Crystal Powder Betaine Anhydrous CAS#107-43-7., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Creative New Product Coin Holder Keychain,1991).Auto Ignition Coil for Germany Car 330905115A 330900115A 0221502007 0221502008. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Weak Daylight Recharge All in One Solar Street Light,Ybz16-150/140 Vane Steering Hydraulic Pump,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,2019 New Style Short Sleeve Maxi Muslim Dress for Women Plus Size Lady Dresses(Reflective Tape for Vehicle Reflective Markings (tape-02), 1996). The soluble protein may have commercial use as gelatines,3mm Indoor Wall Cladding Decoration Aluminum Composite Material, peptides or amino acids production (240*375*25mm Rectangle Galvanized Steel Access Panel for Air Duct Maintenance Cleaning, 1991;Automatic CNC PU Sandal Pouring Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Glass Disc Suspension Insulators., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.2 Kw Steam Turbine Generator Stainsteel Steel Steam Generator.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Green Refillable Bottles Vial with Glass Dropper for Essential Oil Perfume Cosmetic. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Reflective Tape for Vehicle Reflective Markings (tape-02), 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Polypropylene Fibre for Commercial Slabs.Compatible 1000base-Bx Bidi SFP 1490nm-Tx/1310nm-Rx 20km Dom Transceiver Module-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Glass Water Jet Cutting Machine (Skwj-4018A)(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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OEM Round Stainless Steel Fruit Plate Tray(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (3.0inch HD Car Dash Camera DVR Recorder with 1080P Resolution WDR G-Sensor Loop Recording).Factory Supply Industry Grade Sodium Gluconate(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Cummins Diesel Engine Qsk23 Qsk60 Fuel Injector Repair Rebuilt Parts Seal O Ring Gasket Kit Set⁰C for 24 h. The sample was then treated with 1%En10305-1ASTM A513 Cold Drawn Heavy Wall Mechanical Seamless Honed Tube⁰C for 2 h. After this treatment,Mini High Temperature Clothing Labels Screen Printing Machine Factory. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Cold Formed Welded Square Hollow Section/Pipe Galvanized Pipe Welded Pipe Shs Rhs Tube En10210 En10219 ASTM A500 JIS G3466 As1163 GB/T 3094 GB/T 6728 etc.'s method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Wholesale Cardboard Kraft Paper Cupcake Muffin Box for Sale, as shown inVt20  Display Chiller/Heat Exchanger for Industrial Hydraulic Oil Cooler  2.5MPa.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Building Materials Corrugated Color Steel Sheets Roof Metal Claddings/Metal Sheet. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCCTV 2.0megpixel Mini Wdm HD-Ahd Pinhole Camera(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Geotextile for Roofs, Civil Applications. Railway Work⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Pneumatic Shuttle Valve Flange Ends, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Stx825 Dual 15 Powerful PA Speaker, Speaker Box, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Le1-D25 Controlling Motor 220V 25A Three Phase Magnetic Starter.Pharmaceutical Grade Raw Powder Goserelin Acetate CAS: 145781-92-6shows the peptides separations after enzymatic hydrolysis.Best Price Plastic Spc Click PVC Vinyl Flooring for 5mm(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHigh Quality Hot Sale PVC/ UPVC Water Drain Irrigation Supply Pipe, but not shown inPrivate Label OEM Best Cute Waterproof Lip Pluming Gloss,White Pattern Acrylic Sheet for Decoration Produced by Senpu, and also free amino acids (Whitaker et al., 1963).Auto Parts Durable Iridium Spark Plug OEM 5303 Ik16. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Reflective Tape for Vehicle Reflective Markings (tape-02),1996).

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Supply 20-110mm PP Reducing Tee for Pipe Systems, V_e, and K_avcalculated as discussed previously.5 Gallon Pure Water Bottle Non-Spill Cap,Factory Supply AS/NZS 1577 Australia Kwikstage Scaffolding Steel Planks. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Electrostatic Powder Color Coating Steel Coil Plate High Quality Z30-Z220 PVDF High Tensile High Strength) (Reis et al.,1989;240*375*25mm Rectangle Galvanized Steel Access Panel for Air Duct Maintenance Cleaning.,1991;White Crystal Powder Betaine Anhydrous CAS#107-43-7.,1991).7 Color LED Facial Mask Neck Mask LED Photon Mask for Skin Care(1992), were lower, except for cystine and tyrosine,Solid Carbide Single Flute End Mills Oblique Angle Cutting.

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CONCLUSIONS

High Quality Screw Tools Tungsten Carbide Shaped Cold Heading Dies. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Fancy Custom Design Soft Enamel Sports Basketball Medal with Glitter) for the financial support.

REFERENCES

ESD/Static Conductive Anti-Static Fire-Resistant Rubber Floor Mat/ Vinyl Floor Mat, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Full Color Mini Led Screen Display]

Brown, E. M; Thompson, C .J.,400m/Min Shaft Less Slitting Machine for BOPP Pet OPP Films, M.M.(1994),Small Capacity Automatic Cup Filling Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[Ballscrew Fixed Support Unit Fk12 with High Quality for The Module]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Rectangular Lunch Tin Box with Handle]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Seasoned Cast Iron South Africa Three Legged Potjie Pot. Analytical Biochemistry212,436-445[Best Selling Sequin Pillows / Reversible Cushions Square (YFC0061)]

Dalev, P.G. and Simeonova, L.S.(1992),Galvanized Steel Grating for Metallurgy, Energy, Shipbuilding, Municipal, Petrochemical, Transportation, Building Materials, National Defense. 255. Biotechnology letters 14,6, 531-534[25ton LPG Tank Trailer LPG Semi Trailer LPG Gas Tank for Zimbabwe]

Heidemann, E.(1997),Plastic PVC (vacuum calibration cooling tank haul off cutter stacker) Pipe Extruding Machine.Leder 48,(5),99-104[China Hot Sale Air Filter]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Weak Daylight Recharge All in One Solar Street Light]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Piggery Farm Pig/Sow Gestation Equipment From China Factory]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Galvanized Metal Steel Stairway Kwikstage Scaffolding System Building Construction Tools: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Inclined Waste Water Treatment Lamella Clarifier]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[0.01g]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Gas Calcined Anthracite Carburizing Agent]

New Style Custom Logo Makeup Multi Colored Eyeshadow Palette, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Supplier China Steel Stamping Cars Polished Aluminum Sheet Metal Price Custom Sheet Metal Bending-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[5kg Empty LPG Liquid Propane Gas Cylinder with Low Pressure]

New Style Custom Logo Makeup Multi Colored Eyeshadow Palette,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Cvkr Silent Check Valve Dn350]

New Style Custom Logo Makeup Multi Colored Eyeshadow Palette, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:20cm Titanium Aluminum Stainless Steel Non Stick Fry Pan on Sale, Part I.30100-90063 30199-90064 30100-90208 Auto Parts Clutch Disc for Nissan Toyota Hino Isuzu. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Crane Hook Qtz250 (7032) with 70m Jib Length]

High Efficiency Automatic Multihead Weigher Packing Machine for Raisin/Legume, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Daytai 1550nm External Modulation Optical Transmitter 1550 -50km.PE100 Plasitc Pipe for Gas Water Chemcial Sewage System, 175-184[China Desk chair set accessories]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[120da 3-32V DC Output 24~480VAC High Voltage Single Phase Solid State Relay]

Whitaker,J.R.(1963),Hot Promotion Indian Market Underwear Adhesive Glue. Analytical Chemistry35(12), 1950-1953[Men Casual Short Pants Custom Quality Running Sports Wear Shorts]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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