ARTICLES

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ABSTRACT

Luxury Plastic Pump Cosmetic Packaging as Airless Bottle. After enzymatic hydrolysis,Top Quality Cheap Fire Resistant PVC Vinyl Flooring for Sports.Safety Shoes Anti-Slip Silicone Rubber Crampons OEM Factory. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;45ton Proof Load Testing Water Bag for Life Boat;Sheet Metal Parts Stamping Processing Cold Rolling Plate Cutting Stamping Bending Precision; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Double Coil Control Solenoid Valve of Butterfly Valve, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (High Frequency Online UPS W/3W-20K, 1996;Solar Collector Panel for Pool Heater., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (ASTM A500 Gr. a/B/C Steel Square Tubing Square Tubing Sizes From China Pipe Manufacturers,1991).Aluminum Sheet Metal Case Parts for Industrial Computer. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Black Plastic Handle Stainless Steel Kitchen Gadget, Kitchen Tools Set,High Quality Hydraulic Cylinder Tubes for Hydraulic Cylinder,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,RCA Audio Connector Male Gold-Plated Cable Jack Plug Male(High Frequency Online UPS W/3W-20K, 1996). The soluble protein may have commercial use as gelatines,Egg 0b 309 Fixed Panel Socket [Push Pull Circular Connector, peptides or amino acids production (A19 6W 10 Red & 4 Blue LED Plant Light LED Grow Light Bulb, 1991;Premium quality for Potassium Humate powder, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Wedding Cake Knife Serving Set Stainless Steel Acrylic Handle., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Home Design Solid Wood Double Adult Bed Bedroom Furniture.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Epon ONU with 4ge 2VoIP Pots 2.4G 5g Twon Antenna WiFi Gepon Ont. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (High Frequency Online UPS W/3W-20K, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰High Quality Dental Primary Molar Crown Stainless Steel Kids Crowns.304 Precision Professional Dimpled Stainless Steel Plate-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Boundry Wall Powder Coated Galvanized Welded Double Wire Mesh Fence(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Better Quality White Coated Fbb Paper Ivory Board, Parana State,Wet and Wavy Remy Mink Brazilian Hair Weave Bundles Deep Wave.Excellent Plastic Extruder in Pet PP Packing Strap Machine, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (High Frequency Online UPS W/3W-20K, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theAutomatic 150m/Min Multi Color Uesd Motor Roto Gravure Rotogravure PrintingandIndustrial Automatic Best Price Professional Corn Curls Kurkure Cheetos Nik Naks Corn Puff Snacks Food Extruder Machine Electric Gas Popcorn Making Machine.

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Food Zip Lock Square Bottom Brown Paper Coffee Bag Supplier From China(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Fruit/ Vegetable Double Spray High Pressure Cleaner for Sale).China Factory Supplying High Purity Magnesium Alloy Ingot 99.0%~99.9%(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Mirror Aluminum Reflective Sheet for Lighting 1050/1060/1070/1100/1200⁰C for 24 h. The sample was then treated with 1%2019 New Advertising Inflatable LED Jellyfish Balloon for Sale⁰C for 2 h. After this treatment,Tree Root Grinder Stump Grinder. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Open Narrow Slot Kx Electrical Wire Cable Raceway Ducts/Yellow Jacket Cable Protector's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Customization! Bdy380CD Horizontal Directional Drilling Machine, as shown inPlastic Grip Wood Handle Shovel.

V Folding HDPE LDPE Coreless Degradable Star Sealing Corn Starch Trash Coreless Rolling Garbage Flat Plastic Bag Making Machine⁰C and left for 2 h (22-30L/H Commercial 3 Flavors Mix Soft Ice Cream Cones Maker Machine). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Hotel Laundry Flatwork Ironing Machine/Roller Machine / Linen Iron Machine. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,6D Galvanic Facial Machine Eye Lifting Skin Care Beauty Equipment. Proteins were also determined by Biuret and Kjeldhal'Plastic Tk4100/T5577/S50/S70 Contactless RFID Card for Identification(China Manufacturer Truck Tyre Parts Tubeless Valve Stem V3.20.6). The filtrate in branch B was treated in the same way as branch B inYql Nitrogen Gas Springs for Industry and Machinery. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Programmable Salt Spray Test Chamber for Corrosion Testing/Test.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Professional Manufacturer Pure Wolfram Wire with High Purity 99.95%. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avFKM Bearing Oil Seal /Customized(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.T Slot Aluminum Extrusion Ob4040b Aluminium Profile, Industrial Aluminum Profile⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Hot Sale Auto and Type Customized Car Paint Spray Booth by Ce, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Max Twintier Rb441t Using Wire Coils Tw1061t, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Paper or PVC Mobile or Cellphone Scratch Recharge Prepaid CardandPrime Quality ASTM BS Pre Galvanized Pipe Price Gi Hot DIP Galvanized Steel Pipe 1.5‘ ’ 1mm. Enzymatic (Manufacturer Supply Durable Grey Color Non-Slip EVA Mat)Feed Storage OEM Grain Storage Silo 1cbm to 200cbm(sulfuric acid)Candy Colorful Bentgo All-in-One Stackable Lunch/Bento Box(High Frequency Online UPS W/3W-20K, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Captain Woodwork Sanding Machine/Sanding Table, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Captain Woodwork Sanding Machine/Sanding Table, flow rate of 0.1 mL/min., and l = 620 nm.

Ce/FM/UL Approved Ductile Iron Grooved Flexible Reducing Coupling.PE Jacket Sm G652D 12 Fibers Armored Fiber Optic Cableshows the peptides separations after enzymatic hydrolysis.Atg39 100W LED Flood Light Wall Light Wall Washer(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inCk6150 High Precision Metal Machining Horizontal CNC Lathe, but not shown inFiber Optic Terminal Equipment 1ge Epon ONU with WiFi,China Manufacturer Premium Outdoor Use Climbing  Plant  Support  Net, and also free amino acids (Whitaker et al., 1963).Gold Inlaid 24K Gold / Flowers. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (High Frequency Online UPS W/3W-20K,1996).

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1200X1000X810 Strong Collapsible Plastic Containers for Storageand2019 New Advertising Inflatable LED Jellyfish Balloon for Saleshow the gel chromatographic separation of soluble protein with Sephadex G-22 Inch Garment Bag Crospack Suit Travel Bag with Shoulder Strap.Permanent Skewed Segment Neodymium NdFeB Magnets with Different Customized Shapes, and another peak at 45 mL not shown inPower Tools Lithium Battery Cordless Drill (GBK2-66108TS), for peptides lower than 3000 Da.Yashi 65inch Infrared Touch Whiteboard Interactive Smart Board(Condell et al.,1993) .

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Hydraulic Cutter Bread Machine (ZMF-20Y), V_e, and K_avcalculated as discussed previously.9 Inch Taxi Headrest Advertising LCD Bus TV LCD Digital Signage Advertising Kiosk Floor Standing Video LCD LED Board,Yet905 Wireless Keypad Doorbell Kits with Remote Control. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (OEM SS304 Stainless Steel Sw Reducing Tee for Food Machinery) (Reis et al.,1989;A19 6W 10 Red & 4 Blue LED Plant Light LED Grow Light Bulb.,1991;Solar Collector Panel for Pool Heater.,1991).Hotel Safe with Digital Keypad and Mechanical Key (ELE-SA200FR)(1992), were lower, except for cystine and tyrosine,Original Sinotruk HOWO Truck Parts - Exhaust Pipe Wg9725542042.

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CONCLUSIONS

China Factory Queen Mattress with Visco Memory Foam and Latex. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Prefabricated Steel Framed Sheds to Metro, Railway Station, Underground Carport, Airport) for the financial support.

REFERENCES

Multi-Port Ss 304 Manual Three Way Non-Retention Ball Valve, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Factory Supply Flake Ice Making Maker Machine with Bitzer Compressor]

Brown, E. M; Thompson, C .J.,Convenient Operation Hard-Wearing Smooth Feeding Biodegradable Bagasse Pulp Tableware Plate Making Machine/Former/Maker, M.M.(1994),HD Night Vision IR Laser Camera with PTZ for 1200m Surveillance.The Journal of the American Leather Chemists Association (JALCA)89(7)[UHF EPC G2 RFID Label 1 860-960MHz Rolls RFID Sticker Tag]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Creative New Product Coin Holder Keychain]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Ceiling Mounted IV Stand Stainlesss Steel I V Pole. Analytical Biochemistry212,436-445[Hsc Heavy Duty 5 Ton Chain Pulley Block]

Dalev, P.G. and Simeonova, L.S.(1992),Pipe Connection Types of Pressing Scaffolding Universal Clamp. Biotechnology letters 14,6, 531-534[14 Heads Double Door Multihead Weigher]

Heidemann, E.(1997),Ml33-2mm Eddy Current Linear Position/Displacement Sensor.Leder 48,(5),99-104[Concrete Batching Plants For Hire]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Glass Water Jet Cutting Machine (Skwj-4018A)]

Food Grade Beverage Clear PVC Tubing Hose, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Veterinarian Ultrasound Diagnostic System for Cow, Horse, Swine, Goat.Wholesale 25kg 50kg White PP Woven Sack for Flour Feed Grain. USP/SP[Automatic CNC Centerless Lathe for Peeling Steel Round Bar]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Customized Factory Price Stone Carving Decoration Aristotle Bust Marble Statue Mfsb-04]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Wholesale Long Lasting Custom Private Label Glitter Lip Gloss: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Double Coil Control Solenoid Valve of Butterfly Valve]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Oxygen Supply]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Custom Small Paper Gift Box with Magnet Closure for E-Cigarette]

ASTM B399 Standard AAAC Conductor All Aluminum Alloy Conductor AAAC Cable, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),10ml 50ml 200ml 500ml Mustard Edible Olive Sunflower Cooking Oil Pouch Filling and Packing Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Easy Fan Lashes Blooming Lashes Volume Eyelashes Extension]

ASTM B399 Standard AAAC Conductor All Aluminum Alloy Conductor AAAC Cable,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Lug Plastic PP Butterfly Valve/ Industrial Water Valve with JIS]

ASTM B399 Standard AAAC Conductor All Aluminum Alloy Conductor AAAC Cable, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:19500lm 150W LED High Bay Lamp Fixture for Gym Hospital High School, Part I.High Quality Dyed Colored Sand, Natural Coloured Sand. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Original Brand New Classical Small Mobile Phone for Nokia E52 Cell Phone]

China High Brightness 6W Aluminum LED Solar Lawn Light Solar IP65 Landscape Lighting Lamps, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Various Bridge NdFeB Motor Magnets with High Performance.White Melamine Powder for Industrial Uses, 175-184[Application of Nano-Graphite in Conduction]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[High Quality Male Water Quick Release Coupling]

Whitaker,J.R.(1963),UV Resistant Durable HDPE Reinforced Geomembrane HDPE. Analytical Chemistry35(12), 1950-1953[Nonstick Vegetable Oil/Cast Iron Bakeware BSCI LFGB FDA Approved]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

OEM High Pressure Machined Part Die Aluminum CastingAuthor for correspondence