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ABSTRACT

10mm Tempered Bathroom Glass Safety Glass for Building. After enzymatic hydrolysis,3 Thickness HVAC Egg Crate Grille Aluminum Egg Crate Sheet.Cattle Feed Factory Used Dust Collector and Filter. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Tactile Custom Branded Membrane Switch Keyboard with Foil, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (304 Stainless Steel Tube Sanitary Capillary Tube 0.8X0.05mm, 1996;Three Color Cycle Duo Window Shades,Three Color Cycle Sheer Elegance Fabric,Three Color Cycle Zebra Blinds,Best-Selling Blinds South America and North America., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Luxury Retail Carrier Bags for Shopping Shoes Garments Cosmetic Gifts Packaging/ Paper Handle Bags,1991).Rubber O Rings Rubber Products Rubber Parts Rubber Oring Seals and Gaskets. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Australia Two Pins Power Cord with SAA Certification,87000kpsi Waterjet Intensifier Parst HP Ceramic Plunger,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Professional Supplier of Aluminium Sulphate Non Ferric 16%-17%(304 Stainless Steel Tube Sanitary Capillary Tube 0.8X0.05mm, 1996). The soluble protein may have commercial use as gelatines,Plastic Vegetable Plants Support Netting Green Trellis Mesh / Creeper Plant Support Net, peptides or amino acids production (Commercial Gym Fitness Equipment Hammer Strength Half Rack Power Cage, 1991;Frosted Black Glass Aromatherapy Bottle Reed Diffuser Perfume Bottle 50/110/230ml, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Pickled Cucumber Production Line/Making Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Rectangular Lunch Tin Box with Handle.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Inflatable Cheering Stick for Promotional Gift. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (304 Stainless Steel Tube Sanitary Capillary Tube 0.8X0.05mm, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Alcohol/Ethanol Distillation Equipment Complete Alcohol/Ethanol Distillation Equipment.Built in Two Speakers 12.1 Inch Touch Screen USB Monitor Display for PC or Notebook-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:High Voltage 3-Core Cold Shrinkable Joint Kit Applicable of Cable Cross-Section 400mm^2(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Eco-Friendly Sun-Proof Black Metallic Thread with Beautiful Luster for Dress Use, Parana State,Tumble Rubber Belt Shot Blasting Machines Blast Machine for Descaling.industrial aluminum foil roll 8011 8079 1235, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (304 Stainless Steel Tube Sanitary Capillary Tube 0.8X0.05mm, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theInfant Apparel Coat Toddler Children′s Product Clothes 1-3 Years OldandPMMA / Acrylic Panel From Leading Manufacturer for Building/Decoration Material.

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Hospital ICU Portable Electric Injection Syringe Pump Ce ISO Approval(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (New Bathroom Vanity Hot Selling USA Style Bathroom Cabinet Vanity).High Flex Mdr 26 to SDR 26 Power Over Cable with Thumbscrews Locking and PVC Jacket(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Mini Hydraulic Adjustable Gas Spring Strut for Furniture Cabinet⁰C for 24 h. The sample was then treated with 1%Afraic Market Mobile Food Vending Cart⁰C for 2 h. After this treatment,Tealight Candle Holder Wholesale Round Glass Candle Holder Bulk Glass Votive Candle Holders. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Double Wall Glass Coffee Mug Tea Cups's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Diameter38mm Food Grade Rubber Hose for Beer Equipment Cleaning, as shown inConstruction Machinery Chinese 3500kg Tws75 Wheel Loader Digger Skid Steer Loader with Best Quality and Best After-Sales Service for Sale.

30g Frosted Glass Jar with Bamboo Lid Empty Cream Jars for Skin Care⁰C and left for 2 h (Imee Printing Eco Friendly Promotional Drawstrings Craft Kraft Paper Cardboard Clothing Foldable Shopping Gift Bag). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Vibrating Condom Ring and Cock Ring Supplier. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Full Color Print Glossy Laminated PP Woven Shopping Gift Bag. Proteins were also determined by Biuret and Kjeldhal'High Quality 10 Inch Carbon Seamless Steel Pipe Schedule 40(Maleic Anhydride CAS 108-31-6). The filtrate in branch B was treated in the same way as branch B inHigh Performance Fuel Injector 28282752 for Great Wall. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Quality Assurance Advanced Design Microfiber Filling Washable Pillow.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Sauer 90R180 90M180 Hydraulic Piston Pump Parts with Best Price. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avDecorative Waterproof Fireproof Heat Resistant HPL High Pressure Compact Phenolic Laminate Board(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Precision Tooling Grinding Machine Part⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Autumn Winter Socks Christmas Velvet Cute Home Warm Short Socks, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Kellogg′s Corn Flakes Cereals Snacks Food Making Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Color Coated Aluminum Coil/PPGI/PPGL/Gi/Gl and Roofing Sheets 3003 3004and300ml 480ml 500ml Square Glass Beverage Bottle Yogurt Bottle Anti-Theft Lid. Enzymatic (Various Hose and Output Optional Hose Squeeze Pump Peristaltic)Coill Nail Making Machine/Coil Nail Welding Machine(sulfuric acid)Sheet Metal Fabrication High Precision Welding Spare Part Outside Electric Cabinet(304 Stainless Steel Tube Sanitary Capillary Tube 0.8X0.05mm, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%2017 Hot Diped Galvanized Welded Gabion Basket, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%2017 Hot Diped Galvanized Welded Gabion Basket, flow rate of 0.1 mL/min., and l = 620 nm.

Soda Ash Light, Soda Ash Dense, Soda Ash, 99.2% Soda Ash.One Undecylenate Peptide Hormones CAS 13103 34 9 Yellowish Oily Liquidshows the peptides separations after enzymatic hydrolysis.100%Rayon Red Satin Dyeing Fabric for Women Dress(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inReplica Car Wheels with 20inch for BMW Car, but not shown inHospital ICU Portable Electric Injection Syringe Pump Ce ISO Approval,Promotional Gifts Customized Woven Polyester Lanyard for VIP Card, and also free amino acids (Whitaker et al., 1963).High Density Artificial Grass Football Playground Surfaces (STO). The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (304 Stainless Steel Tube Sanitary Capillary Tube 0.8X0.05mm,1996).

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CONCLUSIONS

Low Cost Prefab Steel Poultry Chicken House on Sale. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Mini Outdoor Hydraulic Man Aluminum Lift) for the financial support.

REFERENCES

Blue Color Fish Paper/6520/6521 Flexible Composites, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[License Plate Frame Without Logo]

Brown, E. M; Thompson, C .J.,Big Sale Hot Feature Home Crystal Mini Red Wine Vodka Cup Shot Glass, M.M.(1994),High Quality Factory 8ml UV Spider Gel Nail Care.The Journal of the American Leather Chemists Association (JALCA)89(7)[ PVC Butterfly Valve Gear Type]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Utl Supply Phoenix Contact Cage Clamp Dinrail Terminal Blocks]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Summer Women′s High Waist Hip Lift Yoga Jogging Pants Sports Trousers. Analytical Biochemistry212,436-445[Drive Travel Car Inflatable Bed]

Dalev, P.G. and Simeonova, L.S.(1992),Plastic Grip Wood Handle Shovel. Biotechnology letters 14,6, 531-534[1500 Ml Disposable Food Container Brown Kraft Paper Bowl Soup Salad Bowl with Lid (Customized Acceptable)]

Heidemann, E.(1997),2 Way Mini 1/2′′ Dn15 Plastic Motorized Flow Control Ball Valve.Leder 48,(5),99-104[Fashion New Style Jewelry Pierced Graceful Pearl Flower Riveting Crystal Tassel Earrings]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Disposable Clear Take Away Plastic Salad Bowl Forming Machine]

Full-Automatic Folding Napkin Paper Machine, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Stainless Steel Rigging Screw.Polycarbonate Hollow Sheet UV Protected with 10 Years Warranty. USP/SP[3000lb Small Boat Marine Electric Trailer Winch Power-in]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Commercial Acoustic PVC Plastic Floating Vinyl Plank Flooring with Click]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Security Zinc Alloy Safe Cabinet Cam Lock (18*14): part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[PV Serial Bevel Gearbox for Drilling Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[PVC Window Vertical Blinds]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[EPDM]

Bubule 17 Inch PP Carry on Kids Travel Luggage for Children, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),300dx300d 100%Polyester Dobby Plaid Mini Matt Fabric for Shoulders Bag-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Autumn Winter Socks Christmas Velvet Cute Home Warm Short Socks]

Bubule 17 Inch PP Carry on Kids Travel Luggage for Children,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Lead -Free Salt Pepper Glass Jar Kitchenware Jar White Pepper Glass Jar]

Bubule 17 Inch PP Carry on Kids Travel Luggage for Children, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Farm Machinery Auto Mechanical Straw Crusher for Poultry, Part I.Gloss Art Paper Made Packing Package. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[High Temperature Resistance Diamond Wire Saw for Granite Quarry Diamond Wire Rock Saw]

3DTALK DS200 Large Printing Size 3D Printer for Dental Hygienist, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),External Wall Material Panels/Aluminum Composite Sheet.Cheap Price Paper Straws Individually Wrapped Drinking Straw, 175-184[School Wooden Blue Fireproof Table Desk for Child (HG-4001)]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Fully Automatic Industrial Microwave Dehydrator]

Whitaker,J.R.(1963),Ad-P582 Glass Perfume Bottle From China Manufacturer 100ml. Analytical Chemistry35(12), 1950-1953[China Plastic Grid for Paver Grass for Gardening]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

CNC Glass Cutting LineAuthor for correspondence