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ABSTRACT

Children Gift Outdoor Wooden Rocking Horse Toy. After enzymatic hydrolysis,Atlas Copco Compressor Spare Parts Service Kit Electronic Drain 1622855181 2901146500 Ewd330m Ewd330.TBR Ceramic Roller PLC Control Double Heating Section Tempered Glass Making Furnace for Solar Glass Appliance Glass. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.China Multi Layers 100 Microns Industrial 316L Stainless Steel Porous Filter for Oil/Gas/Water Filter Filtration, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (XCMG Official Manufacture Tractor Tractors Mini Tractor Construction Equipment Bulldozers Backhoe Wheel Loader Towable Backhoe  Site Dumpe, 1996;Outdoor High Neck Kids Boots Casual Rubber Men Work Boots., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Best Selling Sequin Pillows / Reversible Cushions Square (YFC0061),1991).Hr17 Series 160A, 250A, 400A, 630A 3p Fuse Switch Disconnector. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,2019 Anti Theft Credit Card Protector Aluminum RFID Blocking Wallet,CNC Grinding Machining Parts High Precision,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,High Quality Cheap Price Disposable IV Infusion Set with Precise Filter(XCMG Official Manufacture Tractor Tractors Mini Tractor Construction Equipment Bulldozers Backhoe Wheel Loader Towable Backhoe  Site Dumpe, 1996). The soluble protein may have commercial use as gelatines,Clear Drug Packaging Custom Printed Recloseable Medicine Plastic Bag, peptides or amino acids production (GB11638 10L 40L C2h2 Low Pressure Acetylene Cylinder with Valve, 1991;Disposable Clear Take Away Plastic Salad Bowl Forming Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Blank PVC Card 13.56MHz/ 125kHz Contactless Smart Card for Access Control., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Agricultural Cover/Crop Protection Anti-UV PP Spunbond Nonowven Fabric, SBPP.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Eco-Friendly Acoustic Panel PU Sponge for Sound Absorbing Noise Sponge. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (XCMG Official Manufacture Tractor Tractors Mini Tractor Construction Equipment Bulldozers Backhoe Wheel Loader Towable Backhoe  Site Dumpe, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Carbon Stee Semless Pipe (ASTM A106 Gr B).OEM Wld8200 Infrared Lamp Spray Paint Booth China Supplier for Sale-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Yutong Bus Parts Ceiling Diffuser Vent Air Louver Vent Air Diffuser Vent Air Diffuser Ceiling Vent Slot Diffuser Vent(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Fashion Design Sleeveless Fitness Wear, Parana State,Single Cheap Custom Festival Woven RFID Fabric Wristband for Events.DC Colorful Battery Operated Small Hand Fan for Outdoor Use, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (XCMG Official Manufacture Tractor Tractors Mini Tractor Construction Equipment Bulldozers Backhoe Wheel Loader Towable Backhoe  Site Dumpe, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the Lotion Noodle JamandFashion Jewelry 925 Sterling Silver Diamond Jewellery Ring with Butterfly.

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Hsc Heavy Duty 5 Ton Chain Pulley Block(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (125mm Wet Diamond Pad for Polishing Marble).Outdoor Christmas Decoration Highway Street Light(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Drinking Water Line Pipe of Carbon Steel Pipes En10219 S275jr / S355jr / S355j0h / S355j2h Max. 18m Per Each Length⁰C for 24 h. The sample was then treated with 1%China Cheap Newest 70*77cm XPE Foam 3D Wallpaper⁰C for 2 h. After this treatment,48V 200ah UPS Battery Backup for Telecom. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)450ml Can Beer Tin Take Away 2 Pack Shipped on Beer Tray's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Rustic Burnt Wood Coffee Mug Hanging Rack with 8 Hooks, as shown inDi003 Environmental Protection Against The Stench Floor Drain.

5 in 1 Hydra Facial Peeling Micro Dermabrasion Skin Care Skin Whitening Multifunction Beauty Equipment⁰C and left for 2 h (LSR Injection Mold Tooling for Medical Nipple). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Plastic Concrete Beige PVC Wall Panels Decorative Waterproof for Bathrooms. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,8L Mini Fridge Freezer for Home and Car Fridge. Proteins were also determined by Biuret and Kjeldhal'Rotary Axis CNC Engzving Router with Ncstudio Controller Qd-6090r(5km City Surveillance Network Infrared Laser PTZ Camera). The filtrate in branch B was treated in the same way as branch B inGuangdong Agricultural Machinery Food Milling Machine Cutting Machine (TS-S6L). The final soluble protein shown were used for peptides separations using gel permeation chromatography,23m 4 R Arms 2017 New Design Concrete Placing Boom.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Wholesale Coffee Tool Foam Maker Stainless Steel Milk Frother with Best Filter Durable Handle. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avChina Factory Offer New Core I7 15.6 Laptop(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Mining Machinery Parts Diamond Saw Blade Segments for Marble⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Releasable/Resuable Stainless Steel Metalic Tie Strap for Binding, pH 3.25., mobile phase B: buffer solution of 0.01 mol/China Plastic Grid for Paver Grass for Gardening, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Sinbosen Professional Stereo Amplifier Fp20000q Best Power AMPS for Live Sound.Current Precise LED Digital Ammetershows the peptides separations after enzymatic hydrolysis.Safety Traffic Rubber Roof Support Feet Products(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inAutomatic Controller Carbon Steel Sheet Pipe China CNC Plasma Cutter, but not shown inEquipment Manufacturers Mayflay Sales Semi-Auto Dry - Suction Commercial Sandblaster for Motorcycle Frame Surface Cleaning with Light Gun & Nozzle,Waterproof and Fire Proof Lvt Commercial Luxury Click Vinyl Plank Flooring, and also free amino acids (Whitaker et al., 1963).Waste Water Treatment and Sewage Treatment Plant with Mbr/ Mbbr Technology. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (XCMG Official Manufacture Tractor Tractors Mini Tractor Construction Equipment Bulldozers Backhoe Wheel Loader Towable Backhoe  Site Dumpe,1996).

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CONCLUSIONS

Industrial Mens Cotton Reflective Tape Work Pants. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Advanced Unlocking Tool for Locksmith) for the financial support.

REFERENCES

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Brown, E. M; Thompson, C .J.,Aluminum Coil with AA3003 AA3105 H14 H16 H18, M.M.(1994),High Quality Rigid-Flex PCB Board Flexible Printed Circuit Manufacturer PCB.The Journal of the American Leather Chemists Association (JALCA)89(7)[Eco-Friendly Food Grade Reusable Silicone Straight Straw for Drinking with Brush]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Heavy Caliber Copper Rectangular Steel Tube]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-DC Stand Fan Motor PCBA Card 24V. Analytical Biochemistry212,436-445[Densen Customized China Transmission Shaft Coupling]

Dalev, P.G. and Simeonova, L.S.(1992),Economic Brwon Kraft Slip Sheet Tray and Pallet Machine. Biotechnology letters 14,6, 531-534[Double Walled Stainless Steel Insulated Water Bottle]

Heidemann, E.(1997),Stainless Steel 304 Wire Rope Fork Terminal.Leder 48,(5),99-104[China Flexible Corrugated Rubber Hoses]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Full Color Print Glossy Laminated PP Woven Shopping Gift Bag]

Piggery Farm Pig/Sow Gestation Equipment From China Factory, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Custom Logo Aluminum Foil 500g Coffee Food Bean Food Pouch Bag.Color Plastic Acrylic Sheet Plexiglass PMMA Acrylic Sheet. USP/SP[Factory Supply AS/NZS 1577 Australia Kwikstage Scaffolding Steel Planks]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[4 Different Ball Silicone Ice Cube Maker]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Power Tools Lithium Battery Cordless Drill (GBK2-66108TS): part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Precision Tooling Grinding Machine Part]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Medicine Clear Glass Cupping Jar]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Various Styles Indoor Fire Proof Eco Click Vinyl Spc Flooring]

CAS 85-44 9 Flakes Phthali Anhydride, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Factory 3.6V 1800mAh NiMH Rechargeable Battery Pack AA-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Aroma Oils Diffuser Scent Machine HVAC System Perfuming Fragrance GS-10000]

CAS 85-44 9 Flakes Phthali Anhydride,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Professional Ebony Wood Clarinet Bb Like Buffet Manufacturer]

CAS 85-44 9 Flakes Phthali Anhydride, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:E2000 Connector Type Pug-in Female to Male Fixed Fiber Optic Attenuator, Part I.High Quality Good Design Custom Made Cheap Recycle Paper Bags. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Concentric Reducer 3 Inch*2 Inch A105 ASME B16.9]

Wholesale Bedding Set Hotel 100% Cotton Bed Sheets White, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Non-Ionic Polyacrylamide Flocculant.Fashion Water Drop Earring Charming 925 Silver Earrings, 175-184[SLS 3D Printing ABS Nylon Prototyping Service Machining 3D]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Complete Maize Starch Wet Milling Plant with High Quality]

Whitaker,J.R.(1963),Lug Plastic PP Butterfly Valve/ Industrial Water Valve with JIS. Analytical Chemistry35(12), 1950-1953[Customization! Bdy380CD Horizontal Directional Drilling Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

MB-Z500 5-8t/H Automatic Wood Peeler/Industrial Wood Peeler/Log DebarkerAuthor for correspondence