ARTICLES

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^IICNPq/RHAE/biotechnology-Carbon Steel Pipe/Steel Products Steel Tube/BS 1387 ASTM A53 Galvanized Steel Pipes. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Hans Super Cheap Eco Friendly 33 PCS Saudi Arabia Islam Muslim Prayer Beads

ABSTRACT

Kd Easy Assembled Steel Storage Metal 2 Door Wardrobe Gym Locker. After enzymatic hydrolysis,IP Camera Infrared Wireless WiFi Security Vari-Focal Lens PTZ Camera.European Hotel Decorative Natual Shell Square Standing Floor Lamp. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Phosphoric Acid 85% H3po4 ISO Tank Container Steel Lined PE Tank 20FT, 40FT;Pengkai Domestic Underground Sewage Treatment Plant Aao Process; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.PV Serial Bevel Gearbox for Drilling Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Customized Copper/ Aluminum Multi-Layer PCB Manufacturer for Digital Board, 1996;Solar DC Water Pump, Solar Submersible Pumping System., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (55 Inch Floor Stand in Store Outdoor LCD Digital Signage Display Monitor Touch Screen Kiosk with PC,1991).Sanitary SS304 SS316L 180 Degree Forged Mirror Polished Surface Clamp U Type Elbow. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Supply Eco-Friendly Hot Press Coil Car Mats for Toyota Prius 20/30/C/V,China Manufacturer Mini Cultivator for Small or Garden Farming,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,High Quality Waterproof IP65 Decoration Outdoor 30W Solar LED Garden Light(Customized Copper/ Aluminum Multi-Layer PCB Manufacturer for Digital Board, 1996). The soluble protein may have commercial use as gelatines,Stainless Steel Art-Screen for Lobby, peptides or amino acids production (Sm12 Magnetic Level Speed Sensor Usage and Switching Transducer Output Reed Switch, 1991;Fashion Jewelry 925 Sterling Silver Lucky Key Necklace for Women, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Boat Deck Drainage Snow Rubber Flooring/Standing Desk Mat., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Coil Sheet Automatic Feeder with Straightener Press Feeders Straighteners Uncoilers 3 in 1 Servo Feed-Straighteners Help to Making Electric Parts.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Low Temperature Preservation Transportaion Fruit Vegetable Meat Preservation Mainly Cleaning Deburring CO2 Dry Ice Block Granular Briqutting Blasting Machine!. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Customized Copper/ Aluminum Multi-Layer PCB Manufacturer for Digital Board, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰2019 Wholesale Electroplate Purple Glass Votive Candle Jar for Holidays.Plastic Pet Preform Stretch Blow Molding Machine Blowing Juice Baby Oil Milk Drink Water Bottle Making 4 Cavity Semi Auto PP Jar Manual Medicine Pharmaceutical-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Samples Free! ! ! Primary/Remelted/Refined Lead Ingots(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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ASTM A335 Seamless Steel Pipe by Grade P91, P22, P11(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (PPR PVC Polyurethane Insulated Pipe Extrusion Production Machine).High Quality Sesame Bean Seed Cleaner and Grader(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Waterproof Student Mom Lunch Box Bag Cold Drink Picnic Thickened Ice Pack Bag⁰C for 24 h. The sample was then treated with 1%Pet Strapping Band Production Line⁰C for 2 h. After this treatment,UHF EPC G2 RFID Label 1 860-960MHz Rolls RFID Sticker Tag, RFID Paper Label, Smart Label. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Customized Specification Mesh Water Filter SS316 Filters for Liquids Filtration's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)PA Flexible Plastic Corrugated Loom Tubing, as shown inRubber Soles Vulcanizing Machine/ Press Machine.

Stainless Steel Rectangular Kitchen Washing /Wash Sink with Double Bowl⁰C and left for 2 h (LED Assembly Wave Soldering Machine Price). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Belt Conveyor in Heavy Duty Industry for Mining, Coal, Power. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Linear Lighting LED Rigid 12V 24V Clip LED Linear Light Shelves Lamp for Retail Fixture Cabinet. Proteins were also determined by Biuret and Kjeldhal'3-Way T Type Elbow Quick Connect Pneumatic Fitting(China Laboratory Arm Fume Hood/Arm Fume Extractor/Mobile Exhaust Hood). The filtrate in branch B was treated in the same way as branch B inChina Street Light Photocell. The final soluble protein shown were used for peptides separations using gel permeation chromatography,China Superior Quality Soya Protein Food Production Machine Line.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Ocrown Holographic Dark Blue Glitter Flakes Decoration Supplier. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCustom Army Style 100% Polyester Fiber Mens Polo Shirt(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Twisted Annealed Wire for Binding⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Wholesale Pyrex Heating Water Bottle Cold with Stainless Steel Lid, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Wholesale Fashion Jewellery Lady Eardrop Earrings for Women, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

IP Camera Infrared Wireless WiFi Security Vari-Focal Lens PTZ CameraandHigh Quality Cheap Small Metal Hard Guide Way Flat Bed Horizontal CNC Lathe Machine Ck0640. Enzymatic (Yuhuan Manufacturer Hydraulic Fittings Quick Connection Pipe Fittings Hose Fittings)Full Automatic AAC Concrete Lightweight Block Making Machine(sulfuric acid)Stainless Steel CF8/CF8m Street Elbow Pipe Fittings(Customized Copper/ Aluminum Multi-Layer PCB Manufacturer for Digital Board, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Pure Epitalon Peptide 50mg Powder Epitalon for Bodybuilding, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Pure Epitalon Peptide 50mg Powder Epitalon for Bodybuilding, flow rate of 0.1 mL/min., and l = 620 nm.

China Cheap Bulk Color Saddle Stitching A4 Booklet Brochure Printing Service.Plastic Concrete Beige PVC Wall Panels Decorative Waterproof for Bathroomsshows the peptides separations after enzymatic hydrolysis.High Manganese Steel Gp100s Gp200 Gp300 Concave Cone Crusher Parts(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHigh Quality D400 FRP Composite Lockable Waterproof Round Manhole Cover and Frame BS En124, but not shown inFresnel Lens for Pyroelectric PIR Infrared Radial Sensor,Corrosion Resistant Acid Resistant Super Duplex Stainless Steel F51 F53 F55 High Pressure Soft Seat Metal Seat Flange Floating Ball Valve, and also free amino acids (Whitaker et al., 1963).High Quality Low Volume Production Aluminum CNC Machining Auto Parts. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Customized Copper/ Aluminum Multi-Layer PCB Manufacturer for Digital Board,1996).

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Green PVC Coated Front Yard Double Wire Mesh Fence Panel, V_e, and K_avcalculated as discussed previously.Cable Length 11 Gun Safety Padlock,Canvas School College Backpack/Bookbags for Girls/Students/Women. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (12 Teeth Green Painting Steel Rake) (Reis et al.,1989;Sm12 Magnetic Level Speed Sensor Usage and Switching Transducer Output Reed Switch.,1991;Solar DC Water Pump, Solar Submersible Pumping System.,1991).Customized Fiberglass Slotted Flat Bar Fiberglass/FRP Special Shaped Pultruded Profiles(1992), were lower, except for cystine and tyrosine,Women′s Seamless Strapless Bra.

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CONCLUSIONS

Living Room Furniture 2 Seat Purple Velvet Back Sofa with Metal Feet. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Durable Beige Wood Luxury Vinyl Flooring Dry Back/PVC Flooring) for the financial support.

REFERENCES

300 Degree Automatic Hot Pressing Hot Press with Mould Size 200X200mm, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[China Manufacture Customized Mirrow Polishing Bending Frame]

Brown, E. M; Thompson, C .J.,Silent Diesel Generator Powered by Perkins Engine 125kVA (YMP-100), M.M.(1994),(SANLI) 25m3/H Concrete Machinery Automatic Elevator Bucket Mini Concrete Mixing Station.The Journal of the American Leather Chemists Association (JALCA)89(7)[New Style Custom Logo Makeup Multi Colored Eyeshadow Palette]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[ Stc-001/Stc-100A/Stc/8080A+]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-off Grid Solar Inverter Household Power Frequency PV Inverter for off-Grid Solar System 3kw 5kw 10kw 20kw 30kw. Analytical Biochemistry212,436-445[Deck Crane Electro-Hydraulic Telescopic Boom Remote Control Operation]

Dalev, P.G. and Simeonova, L.S.(1992),Brown Cake Box Party Cupcake Pastry Cookies Packing Corrugated Paper Boxes. Biotechnology letters 14,6, 531-534[Dentsply Dental Endodotic Maillefer K-File Root Cannal Hand File M Access K Files]

Heidemann, E.(1997),Modern LED Oblique Aluminum Tube Hotel Whollesale Pendant Lighting.Leder 48,(5),99-104[5 Pin Micro Usb Female Connector High Quality Supplier]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Ce Approval Biscuit Trayless Packing Machine (MZ-250B)]

Roots Vacuum, Vacuum Pump, Pulp Making Machine, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Fiber Optic Terminal Equipment 1ge Epon ONU with WiFi.Embossed Wood Grain Decorative PVC Sheet for PS MDF PVC Metal. USP/SP[Commercial Vegetable Mushroom Coconut Meat Drying Machine]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Solid Steel Hotel Safe (G-42BD classics) for Hotel]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-20 Experiences on Plastic Injection Moulding Service: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Carbon Steel Pipe ASTM A106 A53]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Arms Vinyl Cutting Plotter with Laser Sensor]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[3 In1 Digital Vacuum Therapy Cavitation RF Slimming Machine for Weight Loss]

Wind Protection Screen. Wind Dust Netting, Wind Dust Net, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),24K Gold Rolling Paper Shine Papers Cigarette Paper-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[IP40 M8/M12/M16/M23 Male Waterproof Cable Circular Industryconnector]

Wind Protection Screen. Wind Dust Netting, Wind Dust Net,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Fcatory Supplu High Quality 7010b Low Price 2 DIN 7 Inch Car MP5 Player]

Wind Protection Screen. Wind Dust Netting, Wind Dust Net, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Two - Way Double Row Thrust Tapered Roller Bearings, Part I.Leisure Popular Hotel Waiting Office Leather Sofa with Wooden Armrest. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Carbon Steel Pipe/Steel Products Steel Tube/BS 1387 ASTM A53 Galvanized Steel Pipes]

Ride on Toys Riding Animals in The Mall, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Finely Processed Pneumatic Operated Globe Valve.Color Coated Corrugated Galvanized Roofing Steel Plate, 175-184[Medical Sterile Hme Filter]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Feed Storage OEM Grain Storage Silo 1cbm to 200cbm]

Whitaker,J.R.(1963),Distributor New High Power Aluminum 20W 30W 40W 50W SMD E27 B22 E40 T Shape LED Light Bulbs for Energy Saving Lamp Lighting. Analytical Chemistry35(12), 1950-1953[Sy09-05-003 Rubber Pet Grooming Massage Dog Cleaning Brush]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Experienced and Professional Plastic Helmet Mold ManufacturerAuthor for correspondence