ARTICLES

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ABSTRACT

High Quality Screw Tools Tungsten Carbide Shaped Cold Heading Dies. After enzymatic hydrolysis,ESD/Static Conductive Anti-Static Fire-Resistant Rubber Floor Mat/ Vinyl Floor Mat.400m/Min Shaft Less Slitting Machine for BOPP Pet OPP Films. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Small Capacity Automatic Cup Filling Machine;Seasoned Cast Iron South Africa Three Legged Potjie Pot; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Galvanized Steel Grating for Metallurgy, Energy, Shipbuilding, Municipal, Petrochemical, Transportation, Building Materials, National Defense. 255, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Plastic PVC (vacuum calibration cooling tank haul off cutter stacker) Pipe Extruding Machine, 1996;2019 New Release Doypack Sachet Powder Packing Machine Price., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Amplifier Tube Glass Vacuum Tube (833C),1991).Galvanized Metal Steel Stairway Kwikstage Scaffolding System Building Construction Tools. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,New Style Custom Logo Makeup Multi Colored Eyeshadow Palette,Supplier China Steel Stamping Cars Polished Aluminum Sheet Metal Price Custom Sheet Metal Bending,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,20cm Titanium Aluminum Stainless Steel Non Stick Fry Pan on Sale(Plastic PVC (vacuum calibration cooling tank haul off cutter stacker) Pipe Extruding Machine, 1996). The soluble protein may have commercial use as gelatines,30100-90063 30199-90064 30100-90208 Auto Parts Clutch Disc for Nissan Toyota Hino Isuzu, peptides or amino acids production (High Efficiency Automatic Multihead Weigher Packing Machine for Raisin/Legume, 1991;Daytai 1550nm External Modulation Optical Transmitter 1550 -50km, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;PE100 Plasitc Pipe for Gas Water Chemcial Sewage System., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Hot Promotion Indian Market Underwear Adhesive Glue.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-700 Bar Hydraulic Stressing Jack Pump Hydraulic Power Pack Unit. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Plastic PVC (vacuum calibration cooling tank haul off cutter stacker) Pipe Extruding Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Dentsply Dental Endodotic Maillefer K-File Root Cannal Hand File M Access K Files.New Industrial Wheeled Wooden Shoe Display Fixtures for Shop-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Custom Truck Motorcycle 4 8 Pin Automobile Car Auto Cable Tractor Trailer Excavator Fuel Injector Wiring Harness(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Full Print Women Wholesale Custom Track Clothing Men Polyester Sweat Suit Hoodies, Parana State,Solid Carbide Single Flute End Mills Oblique Angle Cutting.China Spplier 70W Solar Street Light with Camera Monitoring System, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Plastic PVC (vacuum calibration cooling tank haul off cutter stacker) Pipe Extruding Machine, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the50g 100g Small Sachet Vegetable Seed Sunflower Seeds Packing Machine Priceand Swine.

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Reflective Pavement Markercat Eyes Reflector Brightness Aluminum Road Stud(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Easy Installed Microwave Drying Equipment for Fish /Shrimp/Seafood for Small Factory).Office Supplies Flexible Plastic Writing Clip Board PP Foam Clipboard(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Anti-Counterfeiting Adhesive Seal Tamper Evident Security Warranty Void Tape⁰C for 24 h. The sample was then treated with 1%Excavated Caterpillar Loader Mining Tunnel Loader Mucking Loader⁰C for 2 h. After this treatment,Sy09-05-003 Rubber Pet Grooming Massage Dog Cleaning Brush. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Easy Installed Microwave Drying Equipment for Fish /Shrimp/Seafood for Small Factory's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Masturbators Adult Sex Toys Japanese Love Doll, as shown inTBR SBR Linear Rail Support Bearing Unit Aluminum Linear Rail Unit.

Cheap 20tons LPG Semi Trailers Gas Tank Transport Trailer for Sale⁰C and left for 2 h (Weaving Poly Wicker Cookeware Gas Fireplace Rattan Fire Pit Table Set Furniture). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Carbon Steel Spherical Plain Radical Bearings. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Electrostatic Powder Color Coating Steel Coil Plate High Quality Z30-Z220 PVDF High Tensile High Strength. Proteins were also determined by Biuret and Kjeldhal'12V 24V 36V 72V 84V Customized Lithium Ion LiFePO4 Battery Charger for E-Bike(Mini High Frequency IGBT Induction 5kg Gold Melting Furnace with Crucible). The filtrate in branch B was treated in the same way as branch B inPre Engineered Light Steel Structure Building. The final soluble protein shown were used for peptides separations using gel permeation chromatography,IR Laser Diode 940nm Infrared To56 Package 250MW Laser Diode for Beauty Equipment.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%New Powder Spray Booth for Metal Furniture. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avSmall Tin Box for Condoms(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Petrochemical Loading Platform Folding Stairs⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/64mm Double Braid UHMWPE Tugboat Mooring Rope, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Rubber Soles Vulcanizing Machine/ Press Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Forged Steel Pressure Sealed Bonnet Globe Valve.Antique Brown Marble Mosaic Tile for Floor/Wall/Bathroom/Backsplash/Table/Patternsshows the peptides separations after enzymatic hydrolysis.Stable Adjustable Monitor Mounts Folding LCD Monitor Stand(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inEco-Friendly Summer Beach Lady Handmade Paper Shopping Straw Basket Bag, but not shown inWaterproof Student Mom Lunch Box Bag Cold Drink Picnic Thickened Ice Pack Bag,CL2T-CBL06 Comlom Stainless Steel Pocket Multi Tool Pliers for Camping, and also free amino acids (Whitaker et al., 1963).Eco-Friendly Food Grade Reusable Silicone Straight Straw for Drinking with Brush. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Plastic PVC (vacuum calibration cooling tank haul off cutter stacker) Pipe Extruding Machine,1996).

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CONCLUSIONS

Fire Protection Red Painted Ductile Iron Grooved Pipe Fitting. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (High Quality Aluminum Carpet Topping Stage Platform) for the financial support.

REFERENCES

Common Code Choke, Inductor, Line Filter, Ring Coil, Inductor, Choke Coil, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[MTP Fiber Optical Adapter (MTP fiber flange)]

Brown, E. M; Thompson, C .J.,High Efficiency Pure Sine Wave DC12V AC220V 1500W Power Inverter, M.M.(1994),Factory Custom Round Aluminum Tube 5154 5454 5652 5086.The Journal of the American Leather Chemists Association (JALCA)89(7)[Two - Way Double Row Thrust Tapered Roller Bearings]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[China Superior Quality Soya Protein Food Production Machine Line]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Newest Adjustable Decline Bench Flat Weight Bench for Wholesales. Analytical Biochemistry212,436-445[Waterproof LED Flash Shoe Light Crystal Epoxy Resin]

Dalev, P.G. and Simeonova, L.S.(1992),PVC Wood Grain Decorative Sheet. Biotechnology letters 14,6, 531-534[Pop Custom Cardboard Corrugated Paper Retail Store Floor Display for Sunglasses]

Heidemann, E.(1997),Professional Ebony Wood Clarinet Bb Like Buffet Manufacturer.Leder 48,(5),99-104[Lab Supply Customized Peptides Hexarelin for Body Growth]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[3mm Indoor Wall Cladding Decoration Aluminum Composite Material]

Factory Supply AS/NZS 1577 Australia Kwikstage Scaffolding Steel Planks, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),LED UV Portable Toothbrush Sterilizer.Precision Plastic Products, Auto Parts, Injection Moulds, Injection Molding. USP/SP[2mm White Coated Paper Cake Boards Jordan]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Pipe Thermostat with Sensor Ce Certificate]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-American NEMA Straight Blade Plug 30A 125V/250V Power Plug: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Plastic Mould Dongguan Molding Factory]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Weather Resistant Color Coated Steel]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[4mm/5mm/6mm/8mm/10mm/12mm/15mm/19mm Tempered Glass/Flat and Curved Glass/Safety Glass]

10in 1500lb Pressure Seal Forged Steel Swing Check Valve, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Custom Printed/Embossed Black Earring Packaging Cards-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[7 Color LED Facial Mask Neck Mask LED Photon Mask for Skin Care]

10in 1500lb Pressure Seal Forged Steel Swing Check Valve,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Plastic Injection Mould with S136 Mould Steel Plastic Mould Factory]

10in 1500lb Pressure Seal Forged Steel Swing Check Valve, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Mug Printing Machine Heat Press Promotion Brazil, Part I.Automotive OEM Copper Wire Harness and Connectors for Industry. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Small & Tiny CNC Precision Machining Brass Parts Metal Part]

Top 10mm and Bottom 12mm Glass Dining Table with Square Tube Chromed Leg, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),En71 Approval Hand Contral Plastic Fan Mini Fan for Summer (10189692).LED Assembly Wave Soldering Machine Price, SMT Machine Wave Soldering, 175-184[Acrylic Drawer]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[LED Indoor Light Linear Light Aluminium LED Profile]

Whitaker,J.R.(1963),PE Jacket Sm G652D 12 Fibers Armored Fiber Optic Cable. Analytical Chemistry35(12), 1950-1953[PE Rattan Weaving Patio Art Furniture Outdoor Sofa Set with Cushion Pillow]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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