ARTICLES

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ABSTRACT

2 Inch Schedule 40 ASTM A182 316 90d Bw Lr Elbow. After enzymatic hydrolysis,Mini Outdoor Hydraulic Man Aluminum Lift.Cheap Price Roof Wall PU Sandwich Panel Press. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Hospital ICU Portable Electric Injection Syringe Pump Ce ISO Approval, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (High Flex Mdr 26 to SDR 26 Power Over Cable with Thumbscrews Locking and PVC Jacket, 1996;Mini Hydraulic Adjustable Gas Spring Strut for Furniture Cabinet., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Afraic Market Mobile Food Vending Cart,1991).Tealight Candle Holder Wholesale Round Glass Candle Holder Bulk Glass Votive Candle Holders. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Double Wall Glass Coffee Mug Tea Cups,Diameter38mm Food Grade Rubber Hose for Beer Equipment Cleaning,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,30g Frosted Glass Jar with Bamboo Lid Empty Cream Jars for Skin Care(High Flex Mdr 26 to SDR 26 Power Over Cable with Thumbscrews Locking and PVC Jacket, 1996). The soluble protein may have commercial use as gelatines,Vibrating Condom Ring and Cock Ring Supplier, peptides or amino acids production (Full Color Print Glossy Laminated PP Woven Shopping Gift Bag, 1991;High Quality 10 Inch Carbon Seamless Steel Pipe Schedule 40, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Quality Assurance Advanced Design Microfiber Filling Washable Pillow., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Sauer 90R180 90M180 Hydraulic Piston Pump Parts with Best Price.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Decorative Waterproof Fireproof Heat Resistant HPL High Pressure Compact Phenolic Laminate Board. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (High Flex Mdr 26 to SDR 26 Power Over Cable with Thumbscrews Locking and PVC Jacket, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Wall Mounting Metal Control Box Control Desk.Precision Tooling Grinding Machine Part-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Autumn Winter Socks Christmas Velvet Cute Home Warm Short Socks(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Soda Ash Light, Soda Ash Dense, Soda Ash, 99.2% Soda Ash(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Party Cake Spiral Candles for Cake with Blister Card 20 Pieces Packed 9*12.5cm).100%Rayon Red Satin Dyeing Fabric for Women Dress(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Promotional Gifts Customized Woven Polyester Lanyard for VIP Card⁰C for 24 h. The sample was then treated with 1%High Density Artificial Grass Football Playground Surfaces (STO)⁰C for 2 h. After this treatment,High Quality Male Water Quick Release Coupling. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Professional Manufacture Hydraulic Cylinder Tube's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Chenille Microfiber Mop Head, as shown inSelf-Propelled Wheel Farm Using 3 Rows Corn Harvester.

HDPE Auto-Deflashing Plastic Bottle Blowing Machine⁰C and left for 2 h (Stamping Fine Blanking Sheet Metal Fabrication Part). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Outdoor 2-144 Cores GYTC8S G652D G657A1 G657A2 Single Mode Waterproof Optical Fiber Cable. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,High Quality Industrial Grade Anhydrous Sodium Sulfate. Proteins were also determined by Biuret and Kjeldhal'Easy Fan Lashes Blooming Lashes Volume Eyelashes Extension(Weiying Full Computerized Plain and Invisible Sock knitting Machine). The filtrate in branch B was treated in the same way as branch B inCold Rolled Strip. The final soluble protein shown were used for peptides separations using gel permeation chromatography,316L Stainless Steel U Bending Seamless Pipe Tp 304 316L High Pressure U-Bending Tube.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Low Cost Prefab Steel Poultry Chicken House on Sale. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avBlue Color Fish Paper/6520/6521 Flexible Composites(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.High Quality Factory 8ml UV Spider Gel Nail Care⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Summer Women′s High Waist Hip Lift Yoga Jogging Pants Sports Trousers, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Plastic Grip Wood Handle Shovel, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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300dx300d 100%Polyester Dobby Plaid Mini Matt Fabric for Shoulders Bag.Excellent Plastic Extruder in Pet PP Packing Strap Machineshows the peptides separations after enzymatic hydrolysis.Farm Machinery Auto Mechanical Straw Crusher for Poultry(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in for Granule and Powder Selection and Classification, but not shown inHD Night Vision IR Laser Camera with PTZ for 1200m Surveillance,Gloss Art Paper Made Packing Package, and also free amino acids (Whitaker et al., 1963).3DTALK DS200 Large Printing Size 3D Printer for Dental Hygienist. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (High Flex Mdr 26 to SDR 26 Power Over Cable with Thumbscrews Locking and PVC Jacket,1996).

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Medical Cable Assembly Wire Harness Manufacturer, V_e, and K_avcalculated as discussed previously.Hot Sale Wood Transport Log/Timber Truck Trailers for Sale,HCG Pregnancy Test Kits /Rapid Pregnancy Test of Strip Cassette Midstream. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (New Fashion Diaper Bags Mummy Bag) (Reis et al.,1989;Full Color Print Glossy Laminated PP Woven Shopping Gift Bag.,1991;Mini Hydraulic Adjustable Gas Spring Strut for Furniture Cabinet.,1991).Hot Sale AlNiCo Cow Stomach Magnets China Manufacturer(1992), were lower, except for cystine and tyrosine,China Hebei Phosphating Black Heading Machine Drywall Screw.

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CONCLUSIONS

Strong Rare Earth Permanent Wind Driven Generator Magnets. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Various Hose and Output Optional Hose Squeeze Pump Peristaltic) for the financial support.

REFERENCES

Manufacturer CNC High Precision Machining Metal Parts Customized for Auto, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Factory Hot Rolled Ss 321 Stainless Steel Sheet]

Brown, E. M; Thompson, C .J.,China Manufacturer Truck Tyre Parts Tubeless Valve Stem V3.20.6, M.M.(1994),Industry Fluorine Lined Butterfly Valve Lug Type.The Journal of the American Leather Chemists Association (JALCA)89(7)[Plastic Recycling Centrifugal Dewatering Machine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Mosquito Proof Fiberglass Fly Screen]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Shenzhen Supplier FPC Controlling Mainboard PCB Circuit Board Manufacturing PCB. Analytical Biochemistry212,436-445[Food Grade Ivory Paper Cupcake Boxes]

Dalev, P.G. and Simeonova, L.S.(1992),OEM Design Fashion Women Nylon Backpack High Quality Backpacks Custom Backpack. Biotechnology letters 14,6, 531-534[ Sew on Acrylic Mirrors Crystal Flat Back Mirror Beads for Mirror Costume]

Heidemann, E.(1997),Wholesale Magic Barber Personal Use Cordless Rechargeable LCD Hair Clipper.Leder 48,(5),99-104[200kg Cm Drehmomentstarker DC-Motor]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[OEM Round Stainless Steel Fruit Plate Tray]

Built in Two Speakers 12.1 Inch Touch Screen USB Monitor Display for PC or Notebook, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Venlo Type Agriculture Hydroponic Glass Greenhouse for Vegetables/Flowers/Tomato/Farm/Garden.100% PVC Resin PVC Clear Transparent Film for Shower Curtain. USP/SP[Bonfiglioli Ta Series Helical Gearing Gearbox]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Suoer 12V 220V 3000W Pure Sine Wave Inverter (FPC-D3000A)]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Hotel Security Improvement Zinc Alloy Door Chain: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[ Zinc Coated Steel Wire Galvanized Steel Wire]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[neodermalfiller]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Supply 20-110mm PP Reducing Tee for Pipe Systems]

Anti-Pollution Type 160kn Insulator Porclelain Disc, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Nickel 200 Floating Ball Valve-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Slide Switch - DC Power Jack RoHS]

Anti-Pollution Type 160kn Insulator Porclelain Disc,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Cheap Bedroom Furniture Popular Wardrobe Set (CAS-BD1808)]

Anti-Pollution Type 160kn Insulator Porclelain Disc, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:3-Layer PE Coating Production Line, Part I.Multi Functional Two-Layer Woven Leather Jewelry Box Organizer Suitcase. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Tractor Tine Tiller Cultivator]

Surgical Instrument! ! Endoscopic Grasping Forceps for Australia Endoscopy, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Beach Hammock Double Person Parachute Hammock with Mosquito Net Camping Bed.Wooden Queen/King Size Japan Sofa Bed G1162, 175-184[120ml Round Acrylic Liquid Cosmetics Clear Plastic Packaging Bottle]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Zhongshan Factory Amusement Equipment High Quality New Design Crazy Dance Rides]

Whitaker,J.R.(1963),J55 Short Round Thread Seamless Casing Pipe. Analytical Chemistry35(12), 1950-1953[5mm Spc Vinyl Planks Click Lock Floor PVC Plastic Laminate Flooring]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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