ARTICLES

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^IICNPq/RHAE/biotechnology-Cattle Feed Factory Used Dust Collector and Filter. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Custom Clear Foldable Printing PP/PVC/Pet Plastic Packaging Box

ABSTRACT

Group 152cm Picnic Folding in Half Table. After enzymatic hydrolysis,Tactile Custom Branded Membrane Switch Keyboard with Foil.304 Stainless Steel Tube Sanitary Capillary Tube 0.8X0.05mm. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Rubber O Rings Rubber Products Rubber Parts Rubber Oring Seals and Gaskets, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Australia Two Pins Power Cord with SAA Certification, 1996;87000kpsi Waterjet Intensifier Parst HP Ceramic Plunger., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Professional Supplier of Aluminium Sulphate Non Ferric 16%-17%,1991).Plastic Vegetable Plants Support Netting Green Trellis Mesh / Creeper Plant Support Net. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Commercial Gym Fitness Equipment Hammer Strength Half Rack Power Cage,Frosted Black Glass Aromatherapy Bottle Reed Diffuser Perfume Bottle 50/110/230ml,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Pickled Cucumber Production Line/Making Machine(Australia Two Pins Power Cord with SAA Certification, 1996). The soluble protein may have commercial use as gelatines,Rectangular Lunch Tin Box with Handle, peptides or amino acids production (Inflatable Cheering Stick for Promotional Gift, 1991;Alcohol/Ethanol Distillation Equipment Complete Alcohol/Ethanol Distillation Equipment, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Built in Two Speakers 12.1 Inch Touch Screen USB Monitor Display for PC or Notebook., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.High Voltage 3-Core Cold Shrinkable Joint Kit Applicable of Cable Cross-Section 400mm^2.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Customized Stainless Steel Door Lock Strike Plate Metal Lock Bracket. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Australia Two Pins Power Cord with SAA Certification, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰2 Inch Schedule 40 ASTM A182 316 90d Bw Lr Elbow.Mini Outdoor Hydraulic Man Aluminum Lift-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Cheap Price Roof Wall PU Sandwich Panel Press(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Mini Hydraulic Adjustable Gas Spring Strut for Furniture Cabinet, Parana State,Custom Clear Foldable Printing PP/PVC/Pet Plastic Packaging Box.Afraic Market Mobile Food Vending Cart, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Australia Two Pins Power Cord with SAA Certification, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theHigh Quality Bucket Segment Bolt 6V0937and2015 New Square Folding Ajustable Wall LED Bathroom Mirror.

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Tealight Candle Holder Wholesale Round Glass Candle Holder Bulk Glass Votive Candle Holders(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (0.3mm Rotary Cut Red Hardwood Veneer for Making Plywood).Double Wall Glass Coffee Mug Tea Cups(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Diameter38mm Food Grade Rubber Hose for Beer Equipment Cleaning⁰C for 24 h. The sample was then treated with 1%30g Frosted Glass Jar with Bamboo Lid Empty Cream Jars for Skin Care⁰C for 2 h. After this treatment,Vibrating Condom Ring and Cock Ring Supplier. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Full Color Print Glossy Laminated PP Woven Shopping Gift Bag's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)High Quality 10 Inch Carbon Seamless Steel Pipe Schedule 40, as shown inWholesale Soft Hallux Valgus Bunion Corrector Brace for Sport Protection.

Quality Assurance Advanced Design Microfiber Filling Washable Pillow⁰C and left for 2 h (P3.0 576*576mm Display Panel LED for Hotel Church Stage Lighting). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Sauer 90R180 90M180 Hydraulic Piston Pump Parts with Best Price. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Decorative Waterproof Fireproof Heat Resistant HPL High Pressure Compact Phenolic Laminate Board. Proteins were also determined by Biuret and Kjeldhal'Wall Mounting Metal Control Box Control Desk(Children Gift Outdoor Wooden Rocking Horse Toy). The filtrate in branch B was treated in the same way as branch B inPlastic Roofing Tile. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Precision Tooling Grinding Machine Part.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Autumn Winter Socks Christmas Velvet Cute Home Warm Short Socks. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avKellogg′s Corn Flakes Cereals Snacks Food Making Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Various Hose and Output Optional Hose Squeeze Pump Peristaltic⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Coill Nail Making Machine/Coil Nail Welding Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Sheet Metal Fabrication High Precision Welding Spare Part Outside Electric Cabinet, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Summer Women′s High Waist Hip Lift Yoga Jogging Pants Sports Trousers. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Hospital ICU Portable Electric Injection Syringe Pump Ce ISO Approval) for the financial support.

REFERENCES

Plastic Grip Wood Handle Shovel, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[in Rolls Stainless Steel Flat Warp Concertina Razor Barbed Wire]

Brown, E. M; Thompson, C .J.,2 Way Mini 1/2′′ Dn15 Plastic Motorized Flow Control Ball Valve, M.M.(1994),Stainless Steel Rigging Screw.The Journal of the American Leather Chemists Association (JALCA)89(7)[Current Precise LED Digital Ammeter]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Automatic Controller Carbon Steel Sheet Pipe China CNC Plasma Cutter]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Polycarbonate Hollow Sheet UV Protected with 10 Years Warranty. Analytical Biochemistry212,436-445[Equipment Manufacturers Mayflay Sales Semi-Auto Dry - Suction Commercial Sandblaster for Motorcycle Frame Surface Cleaning with Light Gun & Nozzle]

Dalev, P.G. and Simeonova, L.S.(1992),Security Zinc Alloy Safe Cabinet Cam Lock (18*14). Biotechnology letters 14,6, 531-534[Stainless Steel Autoclave UV Sterilizer for Salon Yj-UV3]

Heidemann, E.(1997),Bubule 17 Inch PP Carry on Kids Travel Luggage for Children.Leder 48,(5),99-104[Palm Drying System]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Sport Anti Vibration Hiking Safety Shoes]

3 Thickness HVAC Egg Crate Grille Aluminum Egg Crate Sheet, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),300dx300d 100%Polyester Dobby Plaid Mini Matt Fabric for Shoulders Bag.Farm Machinery Auto Mechanical Straw Crusher for Poultry. USP/SP[Commercial AC Electric Motorized Treadmill 21.5 Touch Screen WiFi HiFi]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Truck Air Dryer cartridge 14bar]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Gloss Art Paper Made Packing Package: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Custom Cheap Marathon Sport Towel Microfiber Slogan Towel for Promotion]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[PS Foam Food Box Vacuum Forming Machine]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[2018 New Design Sea Water 5t Flake Ice Machine with Ice Bin]

3DTALK DS200 Large Printing Size 3D Printer for Dental Hygienist, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),External Wall Material Panels/Aluminum Composite Sheet-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[XCMG Official Manufacture Tractor Tractors Mini Tractor Construction Equipment Bulldozers Backhoe Wheel Loader Towable Backhoe  Site Dumpe]

3DTALK DS200 Large Printing Size 3D Printer for Dental Hygienist,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Horizontal Window Shade Blind Zebra Dual Roller Blinds Day]

3DTALK DS200 Large Printing Size 3D Printer for Dental Hygienist, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Cheap Price Paper Straws Individually Wrapped Drinking Straw, Part I.Ad-P582 Glass Perfume Bottle From China Manufacturer 100ml. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Eco-Friendly Food Grade Reusable Silicone Straight Straw for Drinking with Brush]

Medical Cable Assembly Wire Harness Manufacturer, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Hot Sale Wood Transport Log/Timber Truck Trailers for Sale.HCG Pregnancy Test Kits /Rapid Pregnancy Test of Strip Cassette Midstream, 175-184[Adhesive Tape]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Heavy Caliber Copper Rectangular Steel Tube]

Whitaker,J.R.(1963),Hot Sale AlNiCo Cow Stomach Magnets China Manufacturer. Analytical Chemistry35(12), 1950-1953[Densen Customized China Transmission Shaft Coupling]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Double Walled Stainless Steel Insulated Water BottleAuthor for correspondence