ARTICLES

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ABSTRACT

3PCS OEM Trolley Luggage High Quality PC Travelly Suitcase Bag. After enzymatic hydrolysis,Lint Free Nail Gel Polish Remover Nonwoven Wipes.Shs250X500 Galvanized Square Rectangular Steel Tube Price. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Mechanical Stage Collar Cementing Tool Hydraulic Stage Cementing Tool;Top Quality Inox Plumbing Sanitary Press Fitting to Replace Plastic Pipe Caps Small Plastic Fittings Stainless Steel Spiral Pipe; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Custom Men′s Round Neck Raglan Sleeve Sport T-Shirt, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (From Chongqing by Great Manufacturer Excellent Extrusion and Stamping Die, 1996;Mini Double Hole ABS Plastic CNC Machined Parts Importer., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (KZ-19/16/13 Pneumatic Combination Steel Binding Machine,1991).Medical Packing Self Sealing Sterilization Pouch. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Water Filter Cartridge PP Sediment Filter Cartridge,Wholesale Military Black Color Clothing /Army Bdu Uniforms Surplus Stock for Sale,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Disposable Skin Stapler (CSPF-35W)(From Chongqing by Great Manufacturer Excellent Extrusion and Stamping Die, 1996). The soluble protein may have commercial use as gelatines,Custom Made Restaurant Furniture Artificial Stone Round Coffee Dining Table (190827), peptides or amino acids production (New Customized Mug Heat Press Machine Price, 1991;All-Automatic Powder Wrapping Machine for Tomato Sauce, Lotion Noodle Jam, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Wholesale Plastic Pet Bowl with a Cap Pet Basin Bowl Factory., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Shengya Steel Concrete Electric Pole Mould.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Outdoor Enjoy Music V4.2 Mini Wireless Stereo Bluetooth Speaker. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (From Chongqing by Great Manufacturer Excellent Extrusion and Stamping Die, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Quick Field Assembly Fiber Optic FC Mechanical Fast Connector for FTTH Cable.Wallbox Type 2 32A 22kw Three Phase EV Charging Point EV Charger for Electricvehicles-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:China Manufacturer Garbage Incinerator(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Sany 20ton Mobile Crane Stc200 100 Ton Mobile Crane, Parana State,Rigid PVC/ Rigid Floor /Rigid Flooring /Spc Flooring.Gas Calcined Anthracite Carburizing Agent, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (From Chongqing by Great Manufacturer Excellent Extrusion and Stamping Die, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theHot Sale High Speed Mixer Blender for Extruder MachineandInfant Apparel Coat Toddler Children′s Product Clothes 1-3 Years Old.

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Silver Hammered Round Ball and Natural Stone Necklace(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (2017 New Design Vinyl 3D Stone Wallpaper Factory in China).OEM Box Sealing High Bonding Crystal Super Clear BOPP Packing Tape(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%New Fashion Printed Big Flower Grey Fleece Kangaroo Pocket Sweatshirt Women Long Sleeve Casual Pull Over Slim Fit Hoodies⁰C for 24 h. The sample was then treated with 1%12 Teeth Green Painting Steel Rake⁰C for 2 h. After this treatment,Car Parking or Access Control 2.4 GHz Active RFID Card. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Screw Press Dewatering Machine for Handling The Reject From Paper Mill's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)C Type Small Simple Power Press Tools Machine with Foot Pedal Switch of Manufactuer Suppliers and Importer for Sale, as shown inNew Bathroom Vanity Hot Selling USA Style Bathroom Cabinet Vanity.

4lz-4.5 Small Tank Kubota Similar Rice Harvester⁰C and left for 2 h (Construction Machinery Chinese 3500kg Tws75 Wheel Loader Digger Skid Steer Loader with Best Quality and Best After-Sales Service for Sale). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.High Quality Automatic Snack Food Lab Twin Screw Extruder Price. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Commercial AC Electric Motorized Treadmill 21.5 Touch Screen WiFi HiFi. Proteins were also determined by Biuret and Kjeldhal'IC LED Wire Loop Height Measuring Microscope(Imee Printing Eco Friendly Promotional Drawstrings Craft Kraft Paper Cardboard Clothing Foldable Shopping Gift Bag). The filtrate in branch B was treated in the same way as branch B inSleeveless Outdoor Work Vest. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Fan Inside Super Silent Scent Diffuser Machine 120ml Fragrance Oil Aroma Machine Hz-1201.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Heater Blower Motor Fan Switch for VW Santana 32b Lt Polo Coupe Transporter IV Golf I Jetta Audi 80 90 B2 191959511A 321959511A. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avStainless Steel Whisky Chilling Stones(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.CNC Automatic Pneumatic Tapping Machine/ Drilling Machine for Fasteners⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Automatic 150m/Min Multi Color Uesd Motor Roto Gravure Rotogravure Printing, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Good Quality Custom Car Power Window Cable for Ford Car, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Maleic Anhydride CAS 108-31-6andSolar Panel PV System Mc4 Circular Connector. Enzymatic (1.5 Ton 15 Kn 3300 Lbs Hydraulic Winch for Lifting and Pulling)Hot DIP Galvanized Sewer Cover --Professional Grating Manufacturer(sulfuric acid)Industrial Pouring Ladle Steel Furnace Casting Ladle for Casting(From Chongqing by Great Manufacturer Excellent Extrusion and Stamping Die, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Boiler Fin Tube, Hot Steam Ot Water Cooling ASME SA179 SA192 Fin Tube Wall, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Boiler Fin Tube, Hot Steam Ot Water Cooling ASME SA179 SA192 Fin Tube Wall, flow rate of 0.1 mL/min., and l = 620 nm.

Plastic Recycling Centrifugal Dewatering Machine.Color Coated Aluminum Coil/PPGI/PPGL/Gi/Gl and Roofing Sheets 3003 3004shows the peptides separations after enzymatic hydrolysis.Biostimulant Manufacturer Natural Seaweed Extract Liquid Fertilizer(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inOne Undecylenate Peptide Hormones CAS 13103 34 9 Yellowish Oily Liquid, but not shown inReplica Car Wheels with 20inch for BMW Car,EAS Anti-Theft Optical Security Tag for Glasses Bottle, and also free amino acids (Whitaker et al., 1963).Single Phase Digital Electric Power Meter. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (From Chongqing by Great Manufacturer Excellent Extrusion and Stamping Die,1996).

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CONCLUSIONS

Cheap Original Laptop for DELL Inspiron 13 7000 13.3 FHD Laptop, I7-8550u 8GB 256GB SSD, Win 10. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Square Welded Wire Mesh 1.2m X 30m Galvanized Mesh Roll) for the financial support.

REFERENCES

HVAC Ventilation Supply Air Return Grille Air Vent, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Grey Zro2 Powder For Dental Disks]

Brown, E. M; Thompson, C .J.,High Quality Luxury Design French Style MDF/HDF Molded Wood Door Skin Solid Wooden 2 Panel Wood Door, M.M.(1994),65 Inch LCD Touch Screen Interactive Smart Meeting, Office, Educational Equipment Whiteboard.The Journal of the American Leather Chemists Association (JALCA)89(7)[High Brightness White Color IP20 SMD5050 Chip 30LEDs 7.2W DC24V LED Strip]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[ PVC Butterfly Valve Gear Type]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-High Output Vulcanizing Press Machine. Analytical Biochemistry212,436-445[Utl Supply Phoenix Contact Cage Clamp Dinrail Terminal Blocks]

Dalev, P.G. and Simeonova, L.S.(1992),CO2 Metal Tube Laser Marking Machine for Cloth Leather Plastic Glass. Biotechnology letters 14,6, 531-534[Drive Travel Car Inflatable Bed]

Heidemann, E.(1997),LED Stadium Light High-Mast Outdoor Lighting SMD Sport Lamp Playground Gym Square Football Basketball 300W.Leder 48,(5),99-104[Stainless Steel Fruit Seed Extractor Fruit Seed Remove Machine Passion Fruit Pulping Machine]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[1500 Ml Disposable Food Container Brown Kraft Paper Bowl Soup Salad Bowl with Lid (Customized Acceptable)]

Hot Sale Wholesale Custom Logo Luxury Paper Gift Box with Magnet Closure, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Transmission Ultrasound Gel /20ml, 250ml, 5L.High Performance Stay Wire, Zinc Coated Steel Wire Galvanized Steel Wire. USP/SP[Disposable Clear Take Away Plastic Salad Bowl Forming Machine]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[3000lb Small Boat Marine Electric Trailer Winch Power-in]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Factory Direct Sale PVC Horse Fence/ Powder Coated Rail Fence: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Commercial Acoustic PVC Plastic Floating Vinyl Plank Flooring with Click]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Npk Packaging Machine]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[PV Serial Bevel Gearbox for Drilling Machine]

Food Grade Ivory Paper Cupcake Boxes, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Slide Switch - DC Power Jack RoHS-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[EPDM]

Food Grade Ivory Paper Cupcake Boxes,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Autumn Winter Socks Christmas Velvet Cute Home Warm Short Socks]

Food Grade Ivory Paper Cupcake Boxes, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Factory Custom Fashion High-End Purse Leather Wallet for Women Evening Wedding Party Geldtasche Al370, Part I.2018 New Style High Quality Enamel Bowl with Lid. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Lead -Free Salt Pepper Glass Jar Kitchenware Jar White Pepper Glass Jar]

Roots Vacuum, Vacuum Pump, Pulp Making Machine, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Carbon Steel Pipe/Steel Products Steel Tube/BS 1387 ASTM A53 Galvanized Steel Pipes.Hans Super Cheap Eco Friendly 33 PCS Saudi Arabia Islam Muslim Prayer Beads, 175-184[PLA 1.75mm 3.0mm Filament Single Screw Extruder]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[High Temperature Resistance Diamond Wire Saw for Granite Quarry Diamond Wire Rock Saw]

Whitaker,J.R.(1963),Kd Easy Assembled Steel Storage Metal 2 Door Wardrobe Gym Locker. Analytical Chemistry35(12), 1950-1953[Fully Automatic Industrial Microwave Dehydrator]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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