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ABSTRACT

(SANLI) 25m3/H Concrete Machinery Automatic Elevator Bucket Mini Concrete Mixing Station. After enzymatic hydrolysis,off Grid Solar Inverter Household Power Frequency PV Inverter for off-Grid Solar System 3kw 5kw 10kw 20kw 30kw.Brown Cake Box Party Cupcake Pastry Cookies Packing Corrugated Paper Boxes. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Embossed Wood Grain Decorative PVC Sheet for PS MDF PVC Metal, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (20 Experiences on Plastic Injection Moulding Service, 1996;Wind Protection Screen. Wind Dust Netting, Wind Dust Net., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (24K Gold Rolling Paper Shine Papers Cigarette Paper,1991).Two - Way Double Row Thrust Tapered Roller Bearings. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Leisure Popular Hotel Waiting Office Leather Sofa with Wooden Armrest,Ride on Toys Riding Animals in The Mall,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Finely Processed Pneumatic Operated Globe Valve(20 Experiences on Plastic Injection Moulding Service, 1996). The soluble protein may have commercial use as gelatines,Color Coated Corrugated Galvanized Roofing Steel Plate, peptides or amino acids production (Distributor New High Power Aluminum 20W 30W 40W 50W SMD E27 B22 E40 T Shape LED Light Bulbs for Energy Saving Lamp Lighting, 1991;Custom Personalized Zinc Alloy Engraved Keychains Award Bag Charms, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Precision Diamond Wire Saw Rope for Cutting Concrete Stone., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Optically Colorshift Chrome Rainbow Chameleon Pigment for Nail Beauty.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Blince High Torque OMR Hydro Motor for Injection Moulding Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (20 Experiences on Plastic Injection Moulding Service, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰6 12 18 10 Lead Raw ECG Electrode Cable Placement Used for Patient ECG Monitoring.TV Tower Antenna Supports-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Diamond Silver Ring Silver Plated Zircon Butterfly Shape Women Ring(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

LED Indoor Light Linear Light Aluminium LED Profile, Parana State,Silent Diesel Generator Powered by Perkins Engine 125kVA (YMP-100).50g 100g Small Sachet Vegetable Seed Sunflower Seeds Packing Machine Price, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (20 Experiences on Plastic Injection Moulding Service, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theBest Price Plastic Spc Click PVC Vinyl Flooring for 5mmandAir Compressor Storage Tank Air Compressor Receiver Air Tank (300L).

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Graphic 240X128 Dots Monochrome LCD Display Module(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (CS1363 a Standard UK 3 Pin Power Plug).Dry & Wet Concrete Surface Metal Grinding Wing(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Workbench Anti-Static Table ESD Natural Rubber Table Mat⁰C for 24 h. The sample was then treated with 1%316L 10 Micron Sintered Stainless Steel Filter Disc⁰C for 2 h. After this treatment,Underground Mining Material Handling Belt Conveyor Trough Idler Roller Support/Frame/Bracket. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Abstract Light and Shadow Metal Oil Painting for Deco's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Silo Machine Equipment for Chicken Farm Feed Use (JF-A-L003), as shown in3DTALK FAB460 Large Printing Size 420*420*460mm Industrial Level FDM 3D Printer with Multiple Consumables.

Cheapest Container Living House/Office/Kiosk⁰C and left for 2 h (on-Sale Flat Transparent Glass Jar for Sweet). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Spin Centrifugal Demister Separator or Column Internal. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Baby Wet Wipe Machine. Proteins were also determined by Biuret and Kjeldhal'Closed Impeller Centrifugal Pump Mining Pit Diesel Engine Drive Pump(M23 Round Plug 9 12 Pin Male MID Fixed Signal Connector). The filtrate in branch B was treated in the same way as branch B inASTM 304 304L 316 316L Stainless Steel Sheet / Plate for Ship Building. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Shiny 100% Polyamide 280t 70d Plain Pleat Fabric Jacket Fabric.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%360 Degree Reciprocating Car Wash Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avTemperature Sensor Customized Thermostat for Industrial Appliances(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.High Quality Meat Pie Making Machine with Different Mould Shape⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Gear Operated Flanged High Performance Butterfly Valve, pH 3.25., mobile phase B: buffer solution of 0.01 mol/China Supplier Sinotruk HOWO 6X4 Trailer Head Tractor Truck, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Paci

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Glass Disc Suspension Insulators, V_e, and K_avcalculated as discussed previously.2 Kw Steam Turbine Generator Stainsteel Steel Steam Generator,Green Refillable Bottles Vial with Glass Dropper for Essential Oil Perfume Cosmetic. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (12V Car Cigarette Lighter Adapter Power Extension Cord) (Reis et al.,1989;Distributor New High Power Aluminum 20W 30W 40W 50W SMD E27 B22 E40 T Shape LED Light Bulbs for Energy Saving Lamp Lighting.,1991;Wind Protection Screen. Wind Dust Netting, Wind Dust Net.,1991).Polypropylene Fibre for Commercial Slabs(1992), were lower, except for cystine and tyrosine,Compatible 1000base-Bx Bidi SFP 1490nm-Tx/1310nm-Rx 20km Dom Transceiver Module.

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CONCLUSIONS

Glass Water Jet Cutting Machine (Skwj-4018A). Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Indian Standard Meet Small Kit Prefabricated Modular Container Homes) for the financial support.

REFERENCES

U. S. Type Clevis Grab Hook, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Haivol High Voltage 12/20kv Cold Shrinkable 1 Cores Outdoor Terminal Kit (Applicableof cable crosssection 25)]

Brown, E. M; Thompson, C .J.,Construction Machinery Chinese 3500kg Tws75 Wheel Loader Digger Skid Steer Loader with Best Quality and Best After-Sales Service for Sale, M.M.(1994),Fancy Custom Design Soft Enamel Sports Basketball Medal with Glitter.The Journal of the American Leather Chemists Association (JALCA)89(7)[Breathable Abrasive Resistant Litchi Grain Fabric PVC Poly-Leather for Furniture Sofa Chair Seat Cover Upholstery]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[ 36V]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Car Trunk Organizer Car Storage Organizer. Analytical Biochemistry212,436-445[Temperature Sensor Customized Thermostat for Industrial Appliances]

Dalev, P.G. and Simeonova, L.S.(1992),Fashion Cotton Baby/Kid Hat (DH-BF332). Biotechnology letters 14,6, 531-534[Men′s Outdoor Mountain Hiking Rain Wear Waterproof Jacket with Hood]

Heidemann, E.(1997),Nonwoven Lining Fabric Needle Loom Quilted Lining Fabric Needle Punching Production Line.Leder 48,(5),99-104[Black Bath Towel]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Hot Promotion Indian Market Underwear Adhesive Glue]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[1.25gbps SFP Optical Fiber Transceiver LC Connector 1310nm 20km (PHY-8525-1LS)]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Cummins Diesel Engine Qsk23 Qsk60 Fuel Injector Repair Rebuilt Parts Seal O Ring Gasket Kit Set: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Wind Protection Screen. Wind Dust Netting]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Permanent Suspended Magnetic Separator for Belt Conveyor]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Auto Spare Parts Ignition Coil 22448-2y001]

En10305-1ASTM A513 Cold Drawn Heavy Wall Mechanical Seamless Honed Tube, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Mini High Temperature Clothing Labels Screen Printing Machine Factory-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Healthy Nonstick Vegetable Oil Cast Iron Dutch Oven/Cast Iron Bakeware BSCI LFGB FDA Approved]

En10305-1ASTM A513 Cold Drawn Heavy Wall Mechanical Seamless Honed Tube,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[High Quality 7FT Boundary Wall Fence Design Diamond Mesh Metal Fence]

En10305-1ASTM A513 Cold Drawn Heavy Wall Mechanical Seamless Honed Tube, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Cold Formed Welded Square Hollow Section/Pipe Galvanized Pipe Welded Pipe Shs Rhs Tube En10210 En10219 ASTM A500 JIS G3466 As1163 GB/T 3094 GB/T 6728 etc., Part I.Wholesale Cardboard Kraft Paper Cupcake Muffin Box for Sale. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Bubule 17 Inch PP Carry on Kids Travel Luggage for Children]

Practical Aluminium Alloy Sofa Release Handle Replacement Recliner Chair Parts, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Conventional Best-Selling Household Bamboo Craft Chopsticks.High Quality HDPE Pipe Fitting Flange Cast Iron Plate Flange, 175-184[Unique ABS Wall High Peak Multi-Sides Event Tent]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Tamper Evident Security Seals Heat Resistant Electronic Tape]

Whitaker,J.R.(1963),Ce Marked Medical Syringe Pump (WP08N). Analytical Chemistry35(12), 1950-1953[PVC Wood Grain Decorative Sheet]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Boat Deck Drainage Snow Rubber Flooring/Standing Desk MatAuthor for correspondence