ARTICLES

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ABSTRACT

Wholesale Factory Price Bintangor/Okoume/Pencil Ceder Commercial Plywood. After enzymatic hydrolysis,Johnson Screen Manufacturer Perforated Pipe Galvanized Pipe Screen Filter.Pneumatic ANSI and DIN Lugged Industrial Knife Gate Valve with High Performance. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Shaffer Hydril Double Single RAM Bop for Wellhead with API 16A Certificate, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Glossy and Matte Finish Solid Surface Bathroom Wash Basin Wash Sink, 1996;220V/50Hz Stainless Steel Ozone Generator with 5g/H., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Speedypet Fashionable New Design Oval Pet Products Cat Toilet Box, Cat Toilet Training,1991).Self Adhesive Paper Stickers 13.56MHz NFC Anti-Theft Security RFID Label Tags for Alcoholic. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Luxury Jewelry White Gold Plated Engagement Diamond Ring,Free Sample Cheap Price Plastic Black Underwear Hanger for Sale,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,13.56MHz Nfc Ntag213 Ntag216 RFID Dry Inlay(Glossy and Matte Finish Solid Surface Bathroom Wash Basin Wash Sink, 1996). The soluble protein may have commercial use as gelatines,100% Cotton Face Mask in Guangzhou, peptides or amino acids production (OEM High Pressure Machined Part Die Aluminum Casting, 1991;AISI 430 Ba/2b Embossed Stainless Steel Sheet, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;High Quality 33kv 6.3kv 1000 kVA 50% Copper and 50% Aluminum Material Distribution Transformer Made in China., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Drug Delivery ABS Hospital Medical Treatment Medicine Cart.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-YCOM 1in 2 out HDMI Splitter 4K X 2K for 3D Black. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Glossy and Matte Finish Solid Surface Bathroom Wash Basin Wash Sink, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Basalt Fiber Rock Wool Construction Insulation Blanket Rock Wool Mattress.Colorful Electric English Funny Music Story Module Printing Book-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:2400mm Wood Pulp Waste Paper Corrugated Paper Machine Kraft Liner Paper Making Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

IPL Hair Removal Skin Rejuvenation Beauty Machine (IPL+E-light Function HS-650)Alcalase2.5 L DX,Tilt-and-Turn-with-Hidden-Hinge, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Automatic Soft PVC Granule Used for Refrigerator Door Gasket); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)0.45um Dia 47mm PVDF Filter Membrane(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Commecial Use Watch Display Cabinet for Watch Store Interior Design, Parana State,PE Coated Paper for Salt Sugar Sachets Paper Food Grade.Tunnel Boring Machine Spare Parts Tbm Disc Cutter Ring, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Glossy and Matte Finish Solid Surface Bathroom Wash Basin Wash Sink, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theTent Windows Use China Made PVC Transparent Clear Mesh TarpandAbrasive 6 Sanding Disc for Automotive Industry.

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Slotted Round Head Aluminum Self Tapping Screw(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Hot Sale Electrical Motors Small for Home Appliance).Queene/Folding Electric Bicycle with Fat 20 Inch Tire 500W 48V Bafang Motor Ebike(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Classic Black Color Commercial Glass Door Multi Deck Refrigerator⁰C for 24 h. The sample was then treated with 1%MB-Z500 5-8t/H Automatic Wood Peeler/Industrial Wood Peeler/Log Debarker⁰C for 2 h. After this treatment,Hotbar Reflow Machine for FPC of Camera. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Full Viton Coated Half Shaft Wafer Butterfly Valve's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Claus Process Catalyst 4-6 mm, as shown inGood Excellent Rubber Yoga Mat Making Machine / Rubber Tile Making Machine/Rubber Floor Press.

4 Different Ball Silicone Ice Cube Maker⁰C and left for 2 h (Decorative Waterproof Fireproof Heat Resistant HPL High Pressure Compact Phenolic Laminate Board). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.High Quality Washable Durable Hotel Polyester Banquet Table Cloths. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,16A 32 AMP 3 Pin Plug 220-240V Industrial Plug and Socket Male Female. Proteins were also determined by Biuret and Kjeldhal'Oil Resistant Custom EPDM Rubber O Ring Washer Flange Valve Gasket Oring for Concrete Pump Pipe(OEM/ODM 86 Inch Smart Board IR Iwb Interactive Whiteboard LED LCD Screen for Education and Meetings). The filtrate in branch B was treated in the same way as branch B inCheese Ball Making Machine. The final soluble protein shown were used for peptides separations using gel permeation chromatography,200m Range Video DVR Imagers for Hunting Digital Infrared Night Vision.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Fully Automatic Biodegradable Star Sealing Garbage Bag-on-Roll Plastic Trash Bag Making Machine Manufacturers. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avAerobic Fermentation Organic Fertilizer Agitator(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.CS1363 a Standard UK 3 Pin Power Plug⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Insulation Anti Earthquake Exterior Wall Foam Board, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Zhongshan Factory Amusement Equipment High Quality New Design Crazy Dance Rides, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Raw Material Adhesive NBR Latex of Latex Long GlovesandEasy Soak off OEM 10ml Gel Nail Polish. Enzymatic (Rope Making Machine for PP Danline Hawser Marine Mooring Rope Braiding Machine)Recyclable PU Leather Jewelry Pouch(sulfuric acid)Luxury Special Design Decorative Quality Gold Color Office Round LED Standing Floor Lamp (LT-11)(Glossy and Matte Finish Solid Surface Bathroom Wash Basin Wash Sink, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Activated Alumina Used for Catalyst Carrier in Chemical Industry, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Activated Alumina Used for Catalyst Carrier in Chemical Industry, flow rate of 0.1 mL/min., and l = 620 nm.

High Quality 45W 50W Commercial Recessed COB LED Downlight.Precision Diamond Wire Saw Rope for Cutting Concrete Stoneshows the peptides separations after enzymatic hydrolysis.Toyo-Ta Denso 4 Pin Female Wire Harness Ignition Coil Plug Connector(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inProfessional Manufacturer Pure Wolfram Wire with High Purity 99.95%, but not shown inHDPE Auto-Deflashing Plastic Bottle Blowing Machine,1.25gbps SFP Optical Fiber Transceiver LC Connector 1310nm 20km (PHY-8525-1LS), and also free amino acids (Whitaker et al., 1963).Automatic Liquid Juice Stick Sachet Ice Lolly Pop Packing Machine. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Glossy and Matte Finish Solid Surface Bathroom Wash Basin Wash Sink,1996).

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Indoor Inkjet Media Material, V_e, and K_avcalculated as discussed previously.Steel Jacket Steam Insulation Pipeline Production Famouse in China,Solid Steel Hotel Safe (G-42BD classics) for Hotel. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (2000LTR Horizontal Foam Tank Fire Fighting Suppression) (Reis et al.,1989;OEM High Pressure Machined Part Die Aluminum Casting.,1991;220V/50Hz Stainless Steel Ozone Generator with 5g/H.,1991).Sany Excavator Pn. 11340981 Monitor for Hydraulic Excavator Sy335c/Sy365c/Sy335c(1992), were lower, except for cystine and tyrosine,CNC Aluminum Alloy Metal Stamping Processing Services.

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CONCLUSIONS

Puer/off White Melamine Particle Board/MDF Board, White Board. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Automatic Soft PVC Granule Used for Refrigerator Door Gasket) for the financial support.

REFERENCES

Pet Juice Bottle Cap and Preform Making Automatic Injection Moulding Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Car Pet Beds For Dogs]

Brown, E. M; Thompson, C .J.,2000LTR Horizontal Foam Tank Fire Fighting Suppression, M.M.(1994),High Spped Weaving Machine 6.The Journal of the American Leather Chemists Association (JALCA)89(7)[ 13947]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[External Wall Material Panels/Aluminum Composite Sheet]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Era Valves, PVC Butterfly Valve Gear Type, NSF-Pw & Upc. Analytical Biochemistry212,436-445[Heat Protection Thermal Insulation PTFE Coated Aluminized Fiber Glass Fabric]

Dalev, P.G. and Simeonova, L.S.(1992),Small Poultry Pig Feed Farm Steel Silo for Sale. Biotechnology letters 14,6, 531-534[Wholesale Universal Garden Smart Flower Pot]

Heidemann, E.(1997),Animal/Poultry/Cattle/Cow Silage Grass Feed Cutter Machine.Leder 48,(5),99-104[Yoga Pants & leggings]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Fashion Design Sleeveless Fitness Wear]

Adjustable Grooming Table, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),P3.0 576*576mm Display Panel LED for Hotel Church Stage Lighting.Made in China 12 Inch Aluminum Flexible Duct Pipe. USP/SP[Pure Epitalon Peptide 50mg Powder Epitalon for Bodybuilding]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[99.8% High Aluminium Oxide Low Na2o Calcined Alumina Powder]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-18K White Gold Octagon Emerald Cut Moissanite Diamond Jewelry Engagement Ring: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Widely Use 32 Cavities Pet Preform Water Bottle Mould]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Best Quality Drinking Water Tanks Flexible Water Bladder]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Carrier Roller for Sany Hydraulic Excavator Sy16-Sy850h-8]

Industrial High Pressure Food Quality Rubber Hydualic Hose for Water Food, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Safe Delivery Effective Sarms Mk 677 for Bodybuilding-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[High Manganese Steel Gp100s Gp200 Gp300 Concave Cone Crusher Parts]

Industrial High Pressure Food Quality Rubber Hydualic Hose for Water Food,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[33kv Electrical Automatic Voltage Regulator]

Industrial High Pressure Food Quality Rubber Hydualic Hose for Water Food, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:12# X 14# Electric Galvanized Double Strands Barbed Wire, Part I.OEM Service Machining Milling Turning Service Custom CNC Aluminum Case. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Alloy Steel Hot Forged 4140 42CrMo4 4340 Forging Ring]

Wholesale Mini School Backpack for Girls, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Sy03-05-006 Apple Shape Wallet Silicone Bag Coin Purse.Super Potassium Humate Shiny Flakes, 175-184[Wifi Advertising Player]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[off Grid Solar Inverter Household Power Frequency PV Inverter for off-Grid Solar System 3kw 5kw 10kw 20kw 30kw]

Whitaker,J.R.(1963),4K Giant Whiteboard Interactive Panel for University Whiteboard. Analytical Chemistry35(12), 1950-1953[Economic Brwon Kraft Slip Sheet Tray and Pallet Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

CL1C-GS26-A Comlom Stainless Steel Double-Walled Water BottleAuthor for correspondence