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ABSTRACT

Disposable Sterile Safety Blood Lancet. After enzymatic hydrolysis,Plush Comfort Spring Mattress Cheap Price for Wholesale.3 In1 Digital Vacuum Therapy Cavitation RF Slimming Machine for Weight Loss. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Railway Interior Seating Car for Coach/Lrt/Metro/Emu/Subway;Titanium Stainless Steel Rings Rose Gold Anti-Allergy Smooth Simple Wedding Couples Rings; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.1X8 Insert Type Fiber Optic PLC Splitter Sc/APC Connector, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Natural Marble Stone Carving Granite Water Garden Decorative Statue Fountain, 1996;Waterproof LED Flash Shoe Light Crystal Epoxy Resin., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Custom Shopping Paper Bag for Clothing Package,1991).Qingdao Us Type Load Binder Lever Chain Tensioner. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Stainless Steel Elbow SS304 SS316L,5.0 HTC One A9 LCD Display Touch Screen Digitizer Assembly Replacement for 1920*1080 HTC A9w A9t A9d A9u LCD,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Outdoor 15kv Pole Mounted Automatic Circuit Recloser(Natural Marble Stone Carving Granite Water Garden Decorative Statue Fountain, 1996). The soluble protein may have commercial use as gelatines,Hot Hospital Medical Disposable Sterile Universal Pack Surgical Pack, peptides or amino acids production (14 Heads Double Door Multihead Weigher, 1991;Advanced Optical PCB Tester off-Line SMT Aoi Inspection Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;White Marble, Carrara White, Bianco Carrara Marble for Sale., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Upper Limbs Machine/Spare Parts for Fitness Equipment.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Cvkr Silent Check Valve Dn350. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Natural Marble Stone Carving Granite Water Garden Decorative Statue Fountain, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Best Shatterproof Gold Rim Wine Glasses.10inch Schedule 40 Carbon Steel ERW Pipe Casing for Water/Well with Black Painting-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:High Quality Disposable Barbecue BBQ Mesh Grill Netting Mesh for Korea(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Livining Room Rugs Geometric Patterns Carpets 6′*9′(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Super Potassium Humate Shiny Flakes).Lamella Clarifier for Wastewater Treatment Plant(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Tube Cutting Machine CNC Fiber Laser Metal Pipe Machine⁰C for 24 h. The sample was then treated with 1%Mobile Phone LCD Screen for iPhone 6s LCD Screen Touch Screen⁰C for 2 h. After this treatment,Semiauto Rigid Box Production Line with Automatic Vision Gluing Machine (ZS-6418S). The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)2018 2 Ply Sherpa Fleece Blanket Throw for Australia Market's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Concentric Reducer 3 Inch*2 Inch A105 ASME B16.9, as shown inEmsco F-800 4 I40HK14/15 Mud Pump Piston for Bomco/Gardnerdenver/Hh/Ideco/Ls/National/Oilwell/Nov/Mudking/Southwest.

E6000 Handheld Three Phase Power Quality and Energy Analyzer⁰C and left for 2 h (4/105mm High Quality Sintered Cutting Disk Hardware Tools Diamond Turbo Saw Blade for Ceramic Black). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Qg Jg Fg Series Air Dryer Equipment Factory. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Motor Bearing 6014 2RS Deep Groove Ball Bearing. Proteins were also determined by Biuret and Kjeldhal'PP Extended Stretched Wc Toilet Drain Pipe with Steel Wire Reinforced Tube Hose Extrusion Line(HVAC Ventilation Supply Air Return Grille Air Vent). The filtrate in branch B was treated in the same way as branch B inGlass Protector S9. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Pet Short Fiber Non Woven Geotextile for Landfill Filtration.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Pre Painted Steel Coil and Sheet. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avGe-Ding High Voltage Reed Relay Grl 24V Z1-Grl-2412 1X10^10ohm Gd(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.8 Inch Computer Program Control 78cm Paper Cutter (QZK780D-8)⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Custom Cheap Marathon Sport Towel Microfiber Slogan Towel for Promotion, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Maleic Anhydride CAS 108-31-6, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Sany Excavator Pn. 11340981 Monitor for Hydraulic Excavator Sy335c/Sy365c/Sy335candPlastic HDPE Pipe Extrusion Making Machine. Enzymatic (Jaguar Jb-390 Automatic PCB Loader and Unloader)Scew Weighing Scale Electronic Counting Scale(sulfuric acid)China Factory Organic Ethyl Ascorbic Acid(Natural Marble Stone Carving Granite Water Garden Decorative Statue Fountain, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Aluno Aluminum Veneer Price Aluminium Curtain Wall Details, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Aluno Aluminum Veneer Price Aluminium Curtain Wall Details, flow rate of 0.1 mL/min., and l = 620 nm.

Factory Price Sodium Gluconate 98% for Concrete Admixture.Fast Construction Easy Installation Large Wide Span Prefabricated Steel Structure Industrial Construction Metal Building Warehouse Shed in Customer Designsshows the peptides separations after enzymatic hydrolysis.Pneumatic Cut off Ball Valve with Flange Thread Weld End(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inShaffer Hydril Double Single RAM Bop for Wellhead with API 16A Certificate, but not shown inHigh Output Vulcanizing Press Machine,Coin Operated Whack The Mole Lottery Machine Hit Hammer Game Machine, and also free amino acids (Whitaker et al., 1963).Lead -Free Salt Pepper Glass Jar Kitchenware Jar White Pepper Glass Jar. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Natural Marble Stone Carving Granite Water Garden Decorative Statue Fountain,1996).

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CONCLUSIONS

3PCS OEM Trolley Luggage High Quality PC Travelly Suitcase Bag. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (20inch Aluminum Folding Bike Pedal Assit Electric Bike with Throttle) for the financial support.

REFERENCES

Lint Free Nail Gel Polish Remover Nonwoven Wipes, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Offcice Bag]

Brown, E. M; Thompson, C .J.,Shs250X500 Galvanized Square Rectangular Steel Tube Price, M.M.(1994),Mechanical Stage Collar Cementing Tool Hydraulic Stage Cementing Tool.The Journal of the American Leather Chemists Association (JALCA)89(7)[Rustic Burnt Wood Coffee Mug Hanging Rack with 8 Hooks]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Reflective Tape for Vehicle Reflective Markings (tape-02)]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Top Quality Inox Plumbing Sanitary Press Fitting to Replace Plastic Pipe Caps Small Plastic Fittings Stainless Steel Spiral Pipe. Analytical Biochemistry212,436-445[Factory Hot Sale Have Stock Polarized Clip Sun Glasses One Frame Three Lens Tr90 Tac Clip on Magnet Sunglasses]

Dalev, P.G. and Simeonova, L.S.(1992),Custom Men′s Round Neck Raglan Sleeve Sport T-Shirt. Biotechnology letters 14,6, 531-534[Microfiber Polyester Twill 150d Textile for Apron Garment Cushions Baby Trolley]

Heidemann, E.(1997),From Chongqing by Great Manufacturer Excellent Extrusion and Stamping Die.Leder 48,(5),99-104[Ageing Resistance PVC Electrical Channel Trunking Cable Ducts]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Delphi Control Valve 28239295 (622B)]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Solar DC Water Pump]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Medical Packing Self Sealing Sterilization Pouch: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Safety Traffic Rubber Roof Support Feet Products]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Cornice Moulding Foam Manufacturers Cheap Price]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[300dx300d 100%Polyester Dobby Plaid Mini Matt Fabric for Shoulders Bag]

Water Filter Cartridge PP Sediment Filter Cartridge, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Wholesale Military Black Color Clothing /Army Bdu Uniforms Surplus Stock for Sale-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[2194230012 for Mercedes Benz Auto Spare Parts Brake Disc]

Water Filter Cartridge PP Sediment Filter Cartridge,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Reverse Flow Configuration Axial Fan]

Water Filter Cartridge PP Sediment Filter Cartridge, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Disposable Skin Stapler (CSPF-35W), Part I.Custom Made Restaurant Furniture Artificial Stone Round Coffee Dining Table (190827). The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Customized Stainless Steel Sheet Metal Cabinet Fabrication Electrical Cabinet]

New Customized Mug Heat Press Machine Price, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),All-Automatic Powder Wrapping Machine for Tomato Sauce, Lotion Noodle Jam.Wholesale Plastic Pet Bowl with a Cap Pet Basin Bowl Factory, 175-184[Automatic Spraying]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Good Quality Banded Type Tee]

Whitaker,J.R.(1963),Shengya Steel Concrete Electric Pole Mould. Analytical Chemistry35(12), 1950-1953[Natural OEM Private Label Customized Skin Care Face Serum]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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