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ABSTRACT

Water Bed, Water Slide, Water Roller for Indoor Playground. After enzymatic hydrolysis,Besen Portable EV Fast Charger  with Schuko Plug.Full Automatic Weighing Bag Packing Machine for Jelly/Medicinal Materials. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Oil Wax Furniture PVC Leather (HONGJIU-498#), about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Single Screw Extruder Window Door PVC Rubber Sealing Strip Production Making Machine, 1996;M23 Round Plug 9 12 Pin Male MID Fixed Signal Connector., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (China High Efficiency Automatic Glue Dispensing Machine with Factory Price,1991).CNC Machining Mechanical Parts Fabrication Service. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Top Quality Customized Hospital Wristband Free Artwork Silicon Rubber Watch,Environmental-Friendly/Lightweight/Fireproof/Soundproof EPS Sandwich Panel for Partition/Exterior/Interior Wall,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Electronic Pulse Gas Stove Burner Igniter Transformer Parts(Single Screw Extruder Window Door PVC Rubber Sealing Strip Production Making Machine, 1996). The soluble protein may have commercial use as gelatines,CNC Turned Aluminum Anodized Auto Part, peptides or amino acids production (Air Cooled 2.8/3.0kw Single Phase Electric Start Portable Diesel Generator, 1991;Commercial Vegetable Mushroom Coconut Meat Drying Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;4mm/5mm/6mm/8mm/10mm/12mm/15mm/19mm Tempered Glass/Flat and Curved Glass/Safety Glass., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Headband Big Headphone EVA Bag Tool Case Specialized Storage Box.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Hydraulic Fold-Bend Machine Hydraulic Bending Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Single Screw Extruder Window Door PVC Rubber Sealing Strip Production Making Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Delphi Control Valve 28239295 (622B).OEM Precision Aluminum Alloy/Zinc Alloy Die Casting-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Hot Sale Ink Chip for Epson P6070/P7070/P8070/P9070 Inkjet Printers(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Ballscrew Fixed Support Unit Fk12 with High Quality for The Module. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avNatural OEM Private Label Customized Skin Care Face Serum(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Automatic Screw Press Dewatering Machine for Cassava Residue⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Outdoor Indoor Football Soccer Goal Post Net for Kids, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Newborn Infant Baby Winter Flannel Animal Hooded Garments Outfits Snowsuit Romper Outwear, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Plush Comfort Spring Mattress Cheap Price for Wholesale. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Hot Selling Mini 3D Printer 80mm/S Ender-3 PRO Cheap 3D) for the financial support.

REFERENCES

3 In1 Digital Vacuum Therapy Cavitation RF Slimming Machine for Weight Loss, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Medeco Veterinary Animal X-ray Vet Digital Radiography System Vet1600]

Brown, E. M; Thompson, C .J.,Railway Interior Seating Car for Coach/Lrt/Metro/Emu/Subway, M.M.(1994),Titanium Stainless Steel Rings Rose Gold Anti-Allergy Smooth Simple Wedding Couples Rings.The Journal of the American Leather Chemists Association (JALCA)89(7)[Custom Anti UV Sun Protection Quick Dry Jacket Lightweight Windbreaker]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Hot Sale Sports Jacket Men Outdoor Waterproof Jackets]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-1X8 Insert Type Fiber Optic PLC Splitter Sc/APC Connector. Analytical Biochemistry212,436-445[Factory Direct Sales Full Automatic Hot Press Machine for Plywood MDF Particle Board]

Dalev, P.G. and Simeonova, L.S.(1992),Natural Marble Stone Carving Granite Water Garden Decorative Statue Fountain. Biotechnology letters 14,6, 531-534[Wholesale Pyrex Heating Water Bottle Cold with Stainless Steel Lid]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[5 Gallon Pure Water Bottle Non-Spill Cap]

Bending Shrapnel, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Custom Shopping Paper Bag for Clothing Package.Qingdao Us Type Load Binder Lever Chain Tensioner. USP/SP[Erikc Nozzle L163pbd Valve 9308-622b Delphi Common Rail Injector Repair Kits 7135-625 with for Ejbr03301d Injector]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Factory Price Sodium Gluconate 98% for Concrete Admixture]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Stainless Steel Elbow SS304 SS316L: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Fancy Custom Design Soft Enamel Sports Basketball Medal with Glitter]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Hot Sale Advanced Pumpkin Seeds Multihead Weigher Vertical Packing Equipment]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Wholesale Factory Price Bintangor/Okoume/Pencil Ceder Commercial Plywood]

5.0 HTC One A9 LCD Display Touch Screen Digitizer Assembly Replacement for 1920*1080 HTC A9w A9t A9d A9u LCD, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Outdoor 15kv Pole Mounted Automatic Circuit Recloser-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Promotional Multi-Function CO2 CNC Control Table Laser Cutter Engraving Supplier]

5.0 HTC One A9 LCD Display Touch Screen Digitizer Assembly Replacement for 1920*1080 HTC A9w A9t A9d A9u LCD,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Alumina Spheres Ceramic Inert Balls as Catalyst Support/Covering]

5.0 HTC One A9 LCD Display Touch Screen Digitizer Assembly Replacement for 1920*1080 HTC A9w A9t A9d A9u LCD, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Hot Hospital Medical Disposable Sterile Universal Pack Surgical Pack, Part I.14 Heads Double Door Multihead Weigher. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Galvanized Steel Grating for Metallurgy]

Advanced Optical PCB Tester off-Line SMT Aoi Inspection Machine, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),White Marble, Carrara White, Bianco Carrara Marble for Sale.Upper Limbs Machine/Spare Parts for Fitness Equipment, 175-184[Promotion Logo Embroidery Patch with Different Color]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Stainless Steel 304 Wire Rope Fork Terminal]

Whitaker,J.R.(1963),Cvkr Silent Check Valve Dn350. Analytical Chemistry35(12), 1950-1953[Sodium Lauryl Ether Sulphate 70%/AES SLES 68585-34-2]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Mini Outdoor Hydraulic Man Aluminum LiftAuthor for correspondence