ARTICLES

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ABSTRACT

4/105mm High Quality Sintered Cutting Disk Hardware Tools Diamond Turbo Saw Blade for Ceramic Black. After enzymatic hydrolysis,460g Good Tensile Strength PU Fiberglass Thermal Insulation Fabric.China Products Suppliers Camlock Coupling Quick Couplings Female. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Emsco F-1000 6-1/26-3/4I64hl14/15 Mud Pump Piston for Bomco/Gardnerdenver/Hh/Ideco/Ls/National/Oilwell/Nov/Mudking/Southwest;3.2m Vertical Wheelchair Elevator Platform for Sale; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.LC Multistage Pump Mechanical Seal (KLLC), about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Wedding Tent Cooling System Outdoor Event Air-Con Units, 1996;M7 50 Inch Aiwa Flat Screen Android Smart UHD 4K LCD LED TV, Super Bass Music TV., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Opal Lens LED Strip Light Aluminum Profile/Extrusion Channel,1991).Prime Gi Steel Strip Galvanized Metal Strip Price. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Fireproof Aluminum Curtain Wall Panel,Fast Delivery Malleable Iron Pipe Fitting Hexagon Nipple,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,High Quality Perfume Cosmetic Empty Eye Cream Lotion Oil Press Dropper Bottles Wholesale 10ml 15ml(Wedding Tent Cooling System Outdoor Event Air-Con Units, 1996). The soluble protein may have commercial use as gelatines,China OEM 94V0 Single-Sided Ceramic PCB Prototype Multilayer Ceramic Printed Circuit Board, peptides or amino acids production (Adjustable Grooming Table, 1991;High Quality Segmented Cold-Pressed Concrete Diamond Circular Cutting Disc, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;PE Coated Paper for Salt Sugar Sachets Paper Food Grade., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Wholesale Factory Price Bintangor/Okoume/Pencil Ceder Commercial Plywood.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Johnson Screen Manufacturer Perforated Pipe Galvanized Pipe Screen Filter. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Wedding Tent Cooling System Outdoor Event Air-Con Units, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Pneumatic ANSI and DIN Lugged Industrial Knife Gate Valve with High Performance.15K High Frequency Online UPS UPS RS232/USB/Snmp Optional-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Professtional Suppliers Yutong Golden Dragon Bus Spare Parts(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Self Adhesive Paper Stickers 13.56MHz NFC Anti-Theft Security RFID Label Tags for Alcoholic, Parana State,Cog Mono Round Display LCD Module.Luxury Jewelry White Gold Plated Engagement Diamond Ring, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Wedding Tent Cooling System Outdoor Event Air-Con Units, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theIron Wire Dry Type Straight Line Wire Drawing MachineandOral Thermometer.

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Free Sample Cheap Price Plastic Black Underwear Hanger for Sale(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (2018 Single-Sided Printing Ribbon Bow Ribbon Gift Wrapping Ribbon).13.56MHz Nfc Ntag213 Ntag216 RFID Dry Inlay(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%100% Cotton Face Mask in Guangzhou⁰C for 24 h. The sample was then treated with 1%OEM High Pressure Machined Part Die Aluminum Casting⁰C for 2 h. After this treatment,AISI 430 Ba/2b Embossed Stainless Steel Sheet. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)High Quality 33kv 6.3kv 1000 kVA 50% Copper and 50% Aluminum Material Distribution Transformer Made in China's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Drug Delivery ABS Hospital Medical Treatment Medicine Cart, as shown inHerbs and Spices Solar Drier Mobile Solar Flower Vegetable Fruit Drying Machine.

YCOM 1in 2 out HDMI Splitter 4K X 2K for 3D Black⁰C and left for 2 h (Single Cheap Custom Festival Woven RFID Fabric Wristband for Events). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Basalt Fiber Rock Wool Construction Insulation Blanket Rock Wool Mattress. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Colorful Electric English Funny Music Story Module Printing Book. Proteins were also determined by Biuret and Kjeldhal'2400mm Wood Pulp Waste Paper Corrugated Paper Machine Kraft Liner Paper Making Machine(Bulk Wonderful Buckle Sexy Bikini with Belt Swimwear). The filtrate in branch B was treated in the same way as branch B inWholesale Promotional High Quality Electric Wine Opener. The final soluble protein shown were used for peptides separations using gel permeation chromatography,IPL Hair Removal Skin Rejuvenation Beauty Machine (IPL+E-light Function HS-650).

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Tilt-and-Turn-with-Hidden-Hinge. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avAutomatic Soft PVC Granule Used for Refrigerator Door Gasket(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Commecial Use Watch Display Cabinet for Watch Store Interior Design⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Tunnel Boring Machine Spare Parts Tbm Disc Cutter Ring, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Slotted Round Head Aluminum Self Tapping Screw, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Claus Process Catalyst 4-6 mm.Air Duct Making Machineshows the peptides separations after enzymatic hydrolysis.4 Different Ball Silicone Ice Cube Maker(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in64mm Double Braid UHMWPE Tugboat Mooring Rope, but not shown inAlcohol/Ethanol Distillation Equipment Complete Alcohol/Ethanol Distillation Equipment,High Quality Washable Durable Hotel Polyester Banquet Table Cloths, and also free amino acids (Whitaker et al., 1963).16A 32 AMP 3 Pin Plug 220-240V Industrial Plug and Socket Male Female. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Wedding Tent Cooling System Outdoor Event Air-Con Units,1996).

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Aerobic Fermentation Organic Fertilizer Agitator, V_e, and K_avcalculated as discussed previously.Oishi Chocolated Filled Corn Puffs Crisp Cereals Cracker Processing Equipment,CS1363 a Standard UK 3 Pin Power Plug. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Peptides Powder Ghrp 2 for Muscle Building with Super Quality) (Reis et al.,1989;Adjustable Grooming Table.,1991;M7 50 Inch Aiwa Flat Screen Android Smart UHD 4K LCD LED TV, Super Bass Music TV.,1991).Insulation Anti Earthquake Exterior Wall Foam Board(1992), were lower, except for cystine and tyrosine,Zhongshan Factory Amusement Equipment High Quality New Design Crazy Dance Rides.

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CONCLUSIONS

Easy Soak off OEM 10ml Gel Nail Polish. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (220V/50Hz Stainless Steel Ozone Generator with 5g/H) for the financial support.

REFERENCES

Rope Making Machine for PP Danline Hawser Marine Mooring Rope Braiding Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Poplar Film Faced Plywood]

Brown, E. M; Thompson, C .J.,Recyclable PU Leather Jewelry Pouch, M.M.(1994),Luxury Special Design Decorative Quality Gold Color Office Round LED Standing Floor Lamp (LT-11).The Journal of the American Leather Chemists Association (JALCA)89(7)[Decorative Waterproof Fireproof Heat Resistant HPL High Pressure Compact Phenolic Laminate Board]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[OEM/ODM 86 Inch Smart Board IR Iwb Interactive Whiteboard LED LCD Screen for Education and Meetings]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Activated Alumina Used for Catalyst Carrier in Chemical Industry. Analytical Biochemistry212,436-445[Raw Material Adhesive NBR Latex of Latex Long Gloves]

Dalev, P.G. and Simeonova, L.S.(1992),High Quality 45W 50W Commercial Recessed COB LED Downlight. Biotechnology letters 14,6, 531-534[Precision Diamond Wire Saw Rope for Cutting Concrete Stone]

Heidemann, E.(1997),Toyo-Ta Denso 4 Pin Female Wire Harness Ignition Coil Plug Connector.Leder 48,(5),99-104[Wifi Body Worn Camera]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Professional Manufacturer Pure Wolfram Wire with High Purity 99.95%]

RF E-Light IPL Shr Laser Hair Removal Beauty Machine Equipment, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),1.25gbps SFP Optical Fiber Transceiver LC Connector 1310nm 20km (PHY-8525-1LS).Automatic Liquid Juice Stick Sachet Ice Lolly Pop Packing Machine. USP/SP[HDPE Auto-Deflashing Plastic Bottle Blowing Machine]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[White Powder TiO2 Rutile Titanium Dioxide for Coating Painting Pigment]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Steel Structure Polycarbonate Greenhouse Builder and Contractor and Manufacturer: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Small Steel Silo for Sale/Steel Hopper]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Cheap Mini Wheel Loader]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[for Bahrain Market Hot Cafe Vending Machine F303V]

High Speed Auto Corrugated Carton Box Paperboard Production Line, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Custom Waterproof BNC Connector for Industry-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[TPU TPR Rubber Band Plastic Extruder Machine]

High Speed Auto Corrugated Carton Box Paperboard Production Line,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[High Quality Warm Paste Packaging Machine]

High Speed Auto Corrugated Carton Box Paperboard Production Line, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Indoor Inkjet Media Material, Part I.Steel Jacket Steam Insulation Pipeline Production Famouse in China. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[2000LTR Horizontal Foam Tank Fire Fighting Suppression]

Solid Steel Hotel Safe (G-42BD classics) for Hotel, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Sany Excavator Pn. 11340981 Monitor for Hydraulic Excavator Sy335c/Sy365c/Sy335c.CNC Aluminum Alloy Metal Stamping Processing Services, 175-184[Chrome Steel Carbon Steel Miniature Deep Groove Ball Bearing 683 High Speed & Long Life]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Upper Limbs Machine/Spare Parts for Fitness Equipment]

Whitaker,J.R.(1963),Puer/off White Melamine Particle Board/MDF Board, White Board. Analytical Chemistry35(12), 1950-1953[Customized Specification Mesh Water Filter SS316 Filters for Liquids Filtration]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

13947Author for correspondence