ARTICLES

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ABSTRACT

Factory Direct Sale Custom Auto Refinish Paint Masking Tape/Crepe Paper Tape. After enzymatic hydrolysis,Heavy Caliber Copper Rectangular Steel Tube.2mm White Coated Paper Cake Boards Jordan. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Yuchai 400kw Diesel Generator Set, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Black Plastic GRP FRP PE Pipe Fitting for Water Supply, 1996;2194230012 for Mercedes Benz Auto Spare Parts Brake Disc., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Precision Casting Factory Foundry OEM Ductile Iron Sand Casting,1991).Hot Sale Electrical Motors Small for Home Appliance. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,PE PVC Profile Extruder PE PP Profile Extrusion Machine Plastic Profile Extrusion Line,Bending Shrapnel,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,12V Car Cigarette Lighter Adapter Power Extension Cord(Black Plastic GRP FRP PE Pipe Fitting for Water Supply, 1996). The soluble protein may have commercial use as gelatines,Lab Horizontal Bead Mill for Paint, Ink, Pigment, Agrochemicals, peptides or amino acids production (Water Bed, Water Slide, Water Roller for Indoor Playground, 1991;Besen Portable EV Fast Charger  with Schuko Plug, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Full Automatic Weighing Bag Packing Machine for Jelly/Medicinal Materials., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.1/2′′ X 4feet Hot Dipped Galvanized Welded Mesh Roll.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-UL Kc CB Bis Un38.3 Approved 461730 3.7V 200mAh Lipo Battery for Bluetooth Earphone. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Black Plastic GRP FRP PE Pipe Fitting for Water Supply, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Oil Wax Furniture PVC Leather (HONGJIU-498#).Single Screw Extruder Window Door PVC Rubber Sealing Strip Production Making Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:M23 Round Plug 9 12 Pin Male MID Fixed Signal Connector(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Air Cooled 2.8/3.0kw Single Phase Electric Start Portable Diesel Generator(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Imee Printing Eco Friendly Promotional Drawstrings Craft Kraft Paper Cardboard Clothing Foldable Shopping Gift Bag).Commercial Vegetable Mushroom Coconut Meat Drying Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%4mm/5mm/6mm/8mm/10mm/12mm/15mm/19mm Tempered Glass/Flat and Curved Glass/Safety Glass⁰C for 24 h. The sample was then treated with 1%Headband Big Headphone EVA Bag Tool Case Specialized Storage Box⁰C for 2 h. After this treatment,Hydraulic Fold-Bend Machine Hydraulic Bending Machine. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Delphi Control Valve 28239295 (622B)'s method (Standard Methods for the Examination of Water and Wastewater, 1992); c)OEM Precision Aluminum Alloy/Zinc Alloy Die Casting, as shown inMaleic Anhydride CAS 108-31-6.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Current Precise LED Digital Ammeter. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avAutomatic Drinking Mineral Water Filling Line/Water Bottling Line(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.PP Polypropylene Two Layer Film Blowing Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Disposable Sugarcane Pulp Paper Bowl 500ml, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Flat Roof Insulation Board Heat Insulation Board, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

High Density Insulation Rock Wool Board for Wall. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Top Quality Customized Hospital Wristband Free Artwork Silicon Rubber Watch) for the financial support.

REFERENCES

Garbage Bag Making Machine Price Trash Bag Sealing Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[www.st-package.com]

Brown, E. M; Thompson, C .J.,12 24 Cores Plastic Fiber Optic Fusion Splice Tray for FTTH Cable, M.M.(1994),Finks 41 Inch Spruce Walnut Folk & Acoustic Guitar Special Guitar.The Journal of the American Leather Chemists Association (JALCA)89(7)[Drive Travel Car Inflatable Bed]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[1500 Ml Disposable Food Container Brown Kraft Paper Bowl Soup Salad Bowl with Lid (Customized Acceptable)]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Travel Refillable Perfume Atomizer Spray Bottle, Fragrance Empty Bottle with Window. Analytical Biochemistry212,436-445[Disposable Clear Take Away Plastic Salad Bowl Forming Machine]

Dalev, P.G. and Simeonova, L.S.(1992),Single Block Vertical Wire Drawing Machine for Screw and Bolt Making. Biotechnology letters 14,6, 531-534[3000lb Small Boat Marine Electric Trailer Winch Power-in]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Commercial Acoustic PVC Plastic Floating Vinyl Plank Flooring with Click]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[EPDM]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Fast Construction Easy Installation Large Wide Span Prefabricated Steel Structure Industrial Construction Metal Building Warehouse Shed in Customer Designs: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Autumn Winter Socks Christmas Velvet Cute Home Warm Short Socks]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Magnesium Silicon Master Alloys Supplier & Factory]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Lead -Free Salt Pepper Glass Jar Kitchenware Jar White Pepper Glass Jar]

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Threaded Locking Circular Connectors M16 680,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Fully Automatic Industrial Microwave Dehydrator]

Threaded Locking Circular Connectors M16 680, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Wholesale Price Printable Proximity Em 125kHz Tk4100 Chip RFID Smart ID Card, Part I.Disposable Sterile Safety Blood Lancet. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[China Plastic Grid for Paver Grass for Gardening]

Plush Comfort Spring Mattress Cheap Price for Wholesale, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),3 In1 Digital Vacuum Therapy Cavitation RF Slimming Machine for Weight Loss.Railway Interior Seating Car for Coach/Lrt/Metro/Emu/Subway, 175-184[25ft Embossing Foil]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[CNC Glass Cutting Line]

Whitaker,J.R.(1963),Titanium Stainless Steel Rings Rose Gold Anti-Allergy Smooth Simple Wedding Couples Rings. Analytical Chemistry35(12), 1950-1953[Forged Steel Pressure Sealed Bonnet Globe Valve]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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