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ABSTRACT

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Key words;Pet Juice Bottle Cap and Preform Making Automatic Injection Moulding Machine;2000LTR Horizontal Foam Tank Fire Fighting Suppression; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.High Spped Weaving Machine 6, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Era Valves, PVC Butterfly Valve Gear Type, NSF-Pw & Upc, 1996;Small Poultry Pig Feed Farm Steel Silo for Sale., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Animal/Poultry/Cattle/Cow Silage Grass Feed Cutter Machine,1991).P3.0 576*576mm Display Panel LED for Hotel Church Stage Lighting. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Made in China 12 Inch Aluminum Flexible Duct Pipe,18K White Gold Octagon Emerald Cut Moissanite Diamond Jewelry Engagement Ring,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Industrial High Pressure Food Quality Rubber Hydualic Hose for Water Food(Era Valves, PVC Butterfly Valve Gear Type, NSF-Pw & Upc, 1996). The soluble protein may have commercial use as gelatines,Safe Delivery Effective Sarms Mk 677 for Bodybuilding, peptides or amino acids production (12# X 14# Electric Galvanized Double Strands Barbed Wire, 1991;OEM Service Machining Milling Turning Service Custom CNC Aluminum Case, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Wholesale Mini School Backpack for Girls., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Sy03-05-006 Apple Shape Wallet Silicone Bag Coin Purse.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Super Potassium Humate Shiny Flakes. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Era Valves, PVC Butterfly Valve Gear Type, NSF-Pw & Upc, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰4K Giant Whiteboard Interactive Panel for University Whiteboard.Auto Hydraulic Shock Absorber 48510-09K80 for Toyota Hilux Kun25-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Electric Massage Bed Pedicure Chair with Double Dust Collector(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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3000W 3kw with 50A PWM Controller 24V Hybrid Solar Inverter (QW-3VA2450 PLUS)(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Customized Hardware Aluminum Alloy Die Casting).The Most Demanded Products Fluorescence Microscope Camera(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Low Price Promotion Soda Ash Light to Papermaking⁰C for 24 h. The sample was then treated with 1%Gold Glass Dropper Bottle 20ml 30ml 50ml 100ml Spray Bottle⁰C for 2 h. After this treatment,HOWO 420HP Rhd Tractor Truck Head. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Cummins Generator Set Diesel Engine Spare Parts Fuel Pump Support 4991549's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Decorative Bathroom Illuminated Smart Backlit Rectangle LED Mirror with Blue Tooth, as shown inPolycarbonate Hollow Sheet UV Protected with 10 Years Warranty.

Crankcase Breather Pipe Hose 11157542633 Fit for BMW E87 E90 E91 N46⁰C and left for 2 h (Wholesale Bakeware Metal Stainless Steel Blade Pasta & Pastry Cutter). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.China Supplier AC DC E27 Emergency Rechargeable LED Bulb LED Bulb. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Gaint Inflatable Animal Slide. Proteins were also determined by Biuret and Kjeldhal'White 4*8FT 18mm PVC Foam Board for Cabinets Use(Firefighting Equipment Aluminum Rescue Tripod with Winch 30m). The filtrate in branch B was treated in the same way as branch B in2019 Private Label Professional 10 PCS Makeup Brushes Set. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Dual Function Write Ball Pen Aluminum Stylus for iPhone Xs X Se.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Multi Purpose Microfiber Towel Car / Home / Gift / Sport Towel. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avPower Plant Circulating Water Pump & Spare Part(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Carbon Steel Equal Tee for Connection of Pipe⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Hexagonal Glass Mix Marble Interior Decorative Mosaic Tile, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Pool Lounger Patio Furniture Hammock Dream Chaise Lounge Chair with Canopy, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Vacuum Type Single Facer Corrugator.Mining Machinery Parts Diamond Saw Blade Segments for Marbleshows the peptides separations after enzymatic hydrolysis.Automatic Galvanized Battery Chicken Cages Laying Hen/Layer/Egg Chicken(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inIndustrial High Pressure Food Quality Rubber Hydualic Hose for Water Food, but not shown inHigh Quality Automatic Snack Food Lab Twin Screw Extruder Price,OEM Aluminum Auto Spare High Precision Metal CNC Machinery Mold Parts, and also free amino acids (Whitaker et al., 1963).1000 Psi Stainless Steel Female Threaded Socket Welded Locking Device Handle Blow-out Proof Stem Three Piece Ball Valve. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Era Valves, PVC Butterfly Valve Gear Type, NSF-Pw & Upc,1996).

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CONCLUSIONS

Scantech Handheld Hscan331 3D Laser Scanner for Quality Inspection. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Bgf-800 BOPP Dry Laminating Machine) for the financial support.

REFERENCES

Bluetooth Wireless Earbuds Creative Cute Silicone Earphone Case for Airpods, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Cake Tray Froming Machine]

Brown, E. M; Thompson, C .J.,Indoor Sports Fitness Equipment Electric Manual Motorized Treadmill Exercise Bike Nada Sports, M.M.(1994),Sport Anti Vibration Hiking Safety Shoes.The Journal of the American Leather Chemists Association (JALCA)89(7)[2019 Wholesale Electroplate Purple Glass Votive Candle Jar for Holidays]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Masturbators Adult Sex Toys Japanese Love Doll]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Eco-Friendly Round Head Screw Hex Anchor Eye Bolt for Power Industrial. Analytical Biochemistry212,436-445[Eco-Friendly Sun-Proof Black Metallic Thread with Beautiful Luster for Dress Use]

Dalev, P.G. and Simeonova, L.S.(1992),Semi-Automatic Pipe Cutter CG1-30 Portable Torch Track Burner Straight Line Gas Cutting Machine. Biotechnology letters 14,6, 531-534[LED Fairy Star Light for All Kinds of Decoration Skylike]

Heidemann, E.(1997),24W 400W Rainproof Switch Power Adapter for LED Light.Leder 48,(5),99-104[Cnc Machining Part]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Automotive Sheet Metal Stamping Car Auto Parts]

Indoor Inkjet Media Material, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Industrial Threaded Plastic Connector Female Electrical Plug.Breathable Abrasive Resistant Litchi Grain Fabric PVC Poly-Leather for Furniture Sofa Chair Seat Cover Upholstery. USP/SP[Excavator Transportation Gooseneck Low Bed Semi Trailer 3 Axles]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[3-in-1 Automatic Bottled Pure Mineral Water Filling Equipment for Gallon Bottle]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Modern Style Particle Board Lacquer / PVC Wrapped Vinyl Bathroom Cabinet Vanity: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[China Suppliers Laser Marking Equipment of Fiber Laser]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Auto Parts Automobile Iridium Spark Plug for Toyota]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Manual Double Bridge Glass Cutting Table Machine (JFQG-2620)]

Geotextile for Airport Runways/High Way, Railway, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Single Cylinder Mini ATV with EPA Certificate (MDL GA003-2)-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Hot Selling Fgg. 0b. 309 Metal Circular Push Pull Connector]

Geotextile for Airport Runways/High Way, Railway,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Corrosion Resistant Acid Resistant Super Duplex Stainless Steel F51 F53 F55 High Pressure Soft Seat Metal Seat Flange Floating Ball Valve]

Geotextile for Airport Runways/High Way, Railway, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Color Change LED Restaurant Dinner Chair and Table Restaurant Furniture, Part I.Cute Cartoon Coin Purse Children Zipper Purse. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Pet Juice Bottle Cap and Preform Making Automatic Injection Moulding Machine]

Garment Lace Coating Machine Automatic Silicone Coating Machine, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),2018 New Design Lady Handbag Classic Satchel Women Bag Crossbody (WDL0980).Tamper Evident Security Seals Heat Resistant Electronic Tape, 175-184[Supply GB3030b V Slot Industrial Aluminum Extrusion Profile 6105]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[ Portable Concrete Mixing Plant]

Whitaker,J.R.(1963),South Africa Standards Solar Power Variable Message Sign LED Traffic Sign. Analytical Chemistry35(12), 1950-1953[Silent Diesel Generator Powered by Perkins Engine 125kVA (YMP-100)]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Nonwoven Lining Fabric Needle Loom Quilted Lining Fabric Needle Punching Production LineAuthor for correspondence