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ABSTRACT

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Key words;Rugged Construction Material for Steel Screens for Platforms and Floor Channels.;FKM Rubber Gasket/Rubber O Rings in China; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Inclined Waste Water Treatment Lamella Clarifier, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (33kv 100kVA Dry-Type Distribution Transformer, 1996;Fruit/ Vegetable Double Spray High Pressure Cleaner for Sale., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Fine 36 Hydraulic Breaker Seal Kit for Fine 36 Hydraulic Hammer Seal Kit Fine 36 Breaker Seal Kit,1991).5mm Spc Vinyl Planks Click Lock Floor PVC Plastic Laminate Flooring. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Suoer 12V 220V 3000W Pure Sine Wave Inverter (FPC-D3000A),Rimmed 1 2 5 10 15 20 25 Micron Circular Stainless Steel Filter Disc ,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,China Manufacturer Export Copper Foot Tap/Valve (HFV-001)(33kv 100kVA Dry-Type Distribution Transformer, 1996). The soluble protein may have commercial use as gelatines,Carburetor Adjusting Tool Kit Screwdriver Chainsaw for Poulan Husqvarna Zama Walbro, peptides or amino acids production (PVC Corrugated or Smooth Surface Helix Suction Water Hose, 1991;Cleaning Pipeline Cleaning Rubber Sponge Ball for Concrete Pump Spare Parts, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Ivd Component PP Barbed Threaded Connector., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Workshop Tools 7.5 Ton Electric Winch with Dual Speed.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Best High Energy 1500mj 1064nm 532nm Painless Permanent Age Spot Freckle Tattoo Removal Skin Laser Therapy Equipment. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (33kv 100kVA Dry-Type Distribution Transformer, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Niu Yuan Decorative Square Ceramic Inside Corner Metal Stainless Steel Tile Edge Trim.500ml Stainless Steel Thermos Glass Sport Flask Vacuum Kettle Double Wall Water Bottle-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:RFID Blocking Leather Slim Zipper Credit Card Holder Wallet(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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High Quality D400 FRP Composite Lockable Waterproof Round Manhole Cover and Frame BS En124(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Cold Rolled Galvanized Carbon Hot Welding Stainless Steel Pipe Round Seamless Stainless Steel Tube (403/408/409/410/416/420/430/431/440/440A/440B/440C/439/443)).Marine Anemometer / Wind Meter/ Anemoscope with Alarm Function for Vessel/Ship(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Automotive Sheet Metal Stamping Car Auto Parts⁰C for 24 h. The sample was then treated with 1%Garden Lamp Solar Powered Lighting 48LED PIR Motion Sensor with Remote Control⁰C for 2 h. After this treatment,Indoor Cable 24 Core Multimode/Singlemode Fiber Optic Cable. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)2019 Fashion New Styles Woman Shoulder Hobo Handbags PU Leather Ladies Crossbody Tote Bag W624-1's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Sedimentation Tank/Settling Tank/Lamella Clarifier for Sludge Wastewater separation, as shown inHot Sale Auto and Type Customized Car Paint Spray Booth by Ce.

New Patient Hoist Lift⁰C and left for 2 h (High Sensitivity Ground Metal Detector Searching for Treasure). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.CPVC ASTM2846 Water Supply Pipe Fittings Cross (G23). A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Air Plasma Cutter Lgk100/CNC Plasma Pipe Cutting Machine/Plazma Cutting Machine. Proteins were also determined by Biuret and Kjeldhal'Alumina Spheres Ceramic Inert Balls as Catalyst Support/Covering(High Quality Anti-Corrosion Fiberglass Sheet and Roll). The filtrate in branch B was treated in the same way as branch B inFashion Insulated Double Wall Coffee Cup with PVC Paper Insert. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Best Price 304 Stainless Steel Gas Water Orifice Plate Flow Meter in Kaifeng.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%0.3mm Rotary Cut Red Hardwood Veneer for Making Plywood. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av1080W Most Powerful LED Flood Light Fixtures(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Best Sale 30W 60W 80W 100W 120W LED Intelligent High Power All in One Integrated Solar Lamp Street Light with Light Sensor and Motion Sensor⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/High Quality Bucket Segment Bolt 6V0937, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Best Price Hollow Section Ms ERW Welded Black Steel Pipe for Furniture, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

E6000 Handheld Three Phase Power Quality and Energy AnalyzerandOutdoor Server Rack Network Data Cabinet. Enzymatic (Dedi 43′′ 55′′ Outdoor LCD Display Screen with Camera)Flat Prefab Shipping Container Home Prefabricated Modular Pack Movable House(sulfuric acid)High Quality HDMI to VGA Converter Cable with Audio and USB Power Cable(33kv 100kVA Dry-Type Distribution Transformer, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Hub 113-27-41330 for Bulldozer D31p-18 Spare Parts, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Hub 113-27-41330 for Bulldozer D31p-18 Spare Parts, flow rate of 0.1 mL/min., and l = 620 nm.

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Safety Edge Sensor for Agv Automated Guided Vehicle, V_e, and K_avcalculated as discussed previously.Ultra-Wide Nonmagnetic 5052 Aluminum Alloy Coil for Kitchen Cabinets,Best Design Felt Belt Type Cooling Table/Handling Extrusion System. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Advanced Optical PCB Tester off-Line SMT Aoi Inspection Machine) (Reis et al.,1989;PVC Corrugated or Smooth Surface Helix Suction Water Hose.,1991;Fruit/ Vegetable Double Spray High Pressure Cleaner for Sale.,1991).Men′s Outdoor Mountain Hiking Rain Wear Waterproof Jacket with Hood(1992), were lower, except for cystine and tyrosine,Ycom USB Charger and Data Cable with Aluminum for Type C.

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CONCLUSIONS

Industrial Type Plasma Cutter Machine CNC Metal Plasma Cutting Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Agricultural Mini Tiller Farm Wheel 62cc Cultivator) for the financial support.

REFERENCES

8 Inch H. 265 4K HD IP CCTV Tester Monitor 8MP Ahd Cvi Tvi CVBS SDI Camera Multimeter Optical Fiber Vfl Tdr WiFi Onvif HDMI Input, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Honed Finish Creamy Limestone Floor Tiles]

Brown, E. M; Thompson, C .J.,Seashell Special Shape Magnetic Eye Shadow Empty Palette, M.M.(1994),on-Sale Flat Transparent Glass Jar for Sweet, Honey, Spice Storage.The Journal of the American Leather Chemists Association (JALCA)89(7)[Mobile Computer Workstation Dual Monitors 10-24 Height Adjustment (PC 1602BAS)]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Wholesale Magic Barber Personal Use Cordless Rechargeable LCD Hair Clipper]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Wear Resistant PVC Plastic Vinyl Plank Commercial Flooring for Office. Analytical Biochemistry212,436-445[Hydraulic Three Roll Mill with Hydraulic Squeeze Pressing system]

Dalev, P.G. and Simeonova, L.S.(1992),Nice Quality Coloring Printing Children Board Book Printing. Biotechnology letters 14,6, 531-534[Medical Packing Self Sealing Sterilization Pouch]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Cheap Price Roof Wall PU Sandwich Panel Press]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[380V 3.0HP Peristaltic Pump Swimming Pool Pump]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Microfiber Polyester Twill 150d Textile for Apron Garment Cushions Baby Trolley: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Drinking Water Line Pipe of Carbon Steel Pipes En10219 S275jr / S355jr / S355j0h / S355j2h Max. 18m Per Each Length]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Cheap Shoe Paper Box Packing Manufacturer]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[45ton Proof Load Testing Water Bag for Life Boat]

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Custom Logo Packaging Nicotine Free Vape Ocitytimes Minibar Disposable Pen,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Rubber Tire Plastic Recycling Shredder with Factory Price]

Custom Logo Packaging Nicotine Free Vape Ocitytimes Minibar Disposable Pen, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:300 M Portable Water Well Mining Exploration Core Drilling Rig, Part I.Foteck SSR 40da Solid State Power SSR Relay. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Diesel Truck Engine Parts 3306 Turbocharger for Caterpillar]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[OEM Wld8200 Infrared Lamp Spray Paint Booth China Supplier for Sale]

Whitaker,J.R.(1963),Metal Cutting Machine CNC Plasma Cutter. Analytical Chemistry35(12), 1950-1953[High Quality Stacking Plastic Crystal Bar Stool Chiavari Napoloen Chair]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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