ARTICLES

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ABSTRACT

150*100mm Rearview Mirror Anti Fog Film Rain Proof Oval Film for SUV Car Mirror. After enzymatic hydrolysis,Germany Wool Felt Fedora Trilby Mountain Alpine Hat with Rope and Feather.Cleaning Lint Free Microfiber Mop. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;L2.072.324 Pm74 Sm74 XL75 Hdm Bellows Feeder Dust Cover Length 80mm;Herbs and Spices Solar Drier Mobile Solar Flower Vegetable Fruit Drying Machine; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.LED Emergency Exit Light Rechargeable 220V, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Ss 5 Micron Sinter Powder Liquid Cartridge Filter Stainless Steel Metal Water Filter with M20 M30 M40 Thread Connector, 1996;Firefighting Equipment Aluminum Rescue Tripod with Winch 30m., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Rotor Disc Brake Pad Repair Kit for Wva 29087/29202/29253/29279,1991).Non-Deformation Kitchen Cleaning Sponge Mesh Sponge Scourer. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Automatic Controller Carbon Steel Sheet Pipe China CNC Plasma Cutter,Cjx2-F (LC1-F) AC Magnetic Contactor,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,30W Portable Vehicle Bomb Moving Signal Jammer for GSM/2g/3G/4G Cellular GPS WiFi Signal(Ss 5 Micron Sinter Powder Liquid Cartridge Filter Stainless Steel Metal Water Filter with M20 M30 M40 Thread Connector, 1996). The soluble protein may have commercial use as gelatines,Handmade Stainless Steel Irregular Kitchen Sink Bowl (DF5545XH), peptides or amino acids production (Fj22082mabts 225mm 220V AC Power Amplifier Cooling Fan, 1991;Aluminum/Aluminium Extrusion Profile Housing for LED Light, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Flexible Stand up USB Charging Stands Cable Phone Charger Holder for Apple iPhone Samsung Huawei Android Phone Holders P0.11., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Single Mode Sc/APC Simplex Fiber Optic Adapter.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-120da 3-32V DC Output 24~480VAC High Voltage Single Phase Solid State Relay. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Ss 5 Micron Sinter Powder Liquid Cartridge Filter Stainless Steel Metal Water Filter with M20 M30 M40 Thread Connector, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Double Lines Eco Friendly Automatic Change Rolls HDPE LDPE PLA Corn Starch Trash Garbage Coreless Rolling Smalll Volume Plastic Bag Making Machine.Abrasive 6 Sanding Disc for Automotive Industry-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Hot Sell Advertising Industry Electronics Medicine 30W CO2 Glass Tube Laser Marking Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Indoor Outdoor Playground Ninja Warrior Equipment Obstacle Course(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Aroma Oils Diffuser Scent Machine HVAC System Perfuming Fragrance GS-10000).Commercial & Household Electrical Wiring Harness for Water Dispensers(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Wicker Flower Baskets for Planter⁰C for 24 h. The sample was then treated with 1%5kg Empty LPG Liquid Propane Gas Cylinder with Low Pressure⁰C for 2 h. After this treatment,Manufacturer for All Kinds of Bicycle Accessories/Motorcycle Parts by CNC Centre. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Engine Parts Suspension Control Arm OEM 54501-Jg00b's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Spinning Spin Exercise Bike Fitness Equipment Cross Fit Elliptical Trainer Crosstrainer with Seat, as shown inProfessional Ebony Wood Clarinet Bb Like Buffet Manufacturer.

Custom Anti UV Sun Protection Quick Dry Jacket Lightweight Windbreaker⁰C and left for 2 h (Concentric Reducer 3 Inch*2 Inch A105 ASME B16.9). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Sofiderm Best Selling Breast Enhancer Injectable Hyaluronic Acid Dermal Filler with Ce. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Common Housing Household Plastic Products Injection Moulding Making Machine Manufacturer. Proteins were also determined by Biuret and Kjeldhal'Fiber Laser Marking Machine for Aluminum Alloy, Copper, Iron, Metal Knife(Complete Maize Starch Wet Milling Plant with High Quality). The filtrate in branch B was treated in the same way as branch B in2 Way Output 1550nm External Modulated Optical Transmitter. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Refillable Airless Cosmetic Packaging 10ml 15ml 20ml 30ml Serum Bottle.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Customized Thickness EVA High Density Foam EVA Roll. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avLow Price Cmit/Mit14%/Chloromethyl-Isothiazolone as Bactericide/Fungicide(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Compression Mens Customized Gym Shorts Sportswear⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Hot Sale Prime Hot Dipped Galvanized Steel Coil/Band/Tape/Strip, pH 3.25., mobile phase B: buffer solution of 0.01 mol/99.8% High Aluminium Oxide Low Na2o Calcined Alumina Powder, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

High Quality Stacking Plastic Crystal Bar Stool Chiavari Napoloen Chair. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Single Sphere Rubber Expansion Joint) for the financial support.

REFERENCES

Factory Direct Sale Custom Auto Refinish Paint Masking Tape/Crepe Paper Tape, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[www.yxmembrane.com]

Brown, E. M; Thompson, C .J.,Heavy Caliber Copper Rectangular Steel Tube, M.M.(1994),2mm White Coated Paper Cake Boards Jordan.The Journal of the American Leather Chemists Association (JALCA)89(7)[Top-Grade 100ml Thick Bottom Transparent Mini Glass Wine Bottles for Wine Crafts]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[2018 New Style High Quality Enamel Bowl with Lid]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Multiple Style Jewelry Packaging Box/Ring Box Custom Logo Jewelry Box. Analytical Biochemistry212,436-445[Ultra Powerful Waterproof 10 Speed Silicone Relaxation Therapeutic Massager]

Dalev, P.G. and Simeonova, L.S.(1992),Luxious Hotel Passenger Elevator, Titanium Elevator. Biotechnology letters 14,6, 531-534[2018 Cheap PU Leather Bracelet in Alloy Jewelry]

Heidemann, E.(1997),Yuchai 400kw Diesel Generator Set.Leder 48,(5),99-104[Customize Powder Coating Steel Stamping Terminal Cable Equipment Mounting Bracket]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Built in Two Speakers 12.1 Inch Touch Screen USB Monitor Display for PC or Notebook]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Spin Centrifugal Demister Separator or Column Internal]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Precision Casting Factory Foundry OEM Ductile Iron Sand Casting: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Kellogg′s Corn Flakes Cereals Snacks Food Making Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[WPC Wood-Plastic Composites Decoration Profile Making Machine]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Tongkat Ali Root Extract 200 1]

Hot Sale Electrical Motors Small for Home Appliance, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),PE PVC Profile Extruder PE PP Profile Extrusion Machine Plastic Profile Extrusion Line-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Ad-P582 Glass Perfume Bottle From China Manufacturer 100ml]

Hot Sale Electrical Motors Small for Home Appliance,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Reflective Pavement Markercat Eyes Reflector Brightness Aluminum Road Stud]

Hot Sale Electrical Motors Small for Home Appliance, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Bending Shrapnel, Part I.12V Car Cigarette Lighter Adapter Power Extension Cord. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Barricade Tape]

Lab Horizontal Bead Mill for Paint, Ink, Pigment, Agrochemicals, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Water Bed, Water Slide, Water Roller for Indoor Playground.Besen Portable EV Fast Charger  with Schuko Plug, 175-184[2020 Newest 2000 Meter Laser Round 7 Inch High Low Beam Truck Auto Head Lights Laser LED Driving Lights]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Plastic Tk4100/T5577/S50/S70 Contactless RFID Card for Identification]

Whitaker,J.R.(1963),Full Automatic Weighing Bag Packing Machine for Jelly/Medicinal Materials. Analytical Chemistry35(12), 1950-1953[Stainless Steel Investment Precision Casting for Valve Body with Machining]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

4 Heads High Speed Professional Paper Cardboard Laser CutterAuthor for correspondence