ARTICLES

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ABSTRACT

Pulse Output Vortex Flow Meter with Flanged Connection Wate Meter. After enzymatic hydrolysis,Cosmetics Facial Cream Toothpaste Tube Filling Sealing Machine.Customized Logo Eco-Friendly Orange Cotton Shoulder Shopping Bag. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Bosch Alternator 0124515010 Sg12b015 Lester: 13853, 13942, 13947, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (RF E-Light IPL Shr Laser Hair Removal Beauty Machine Equipment, 1996;Prepainted Corrugated Gi Color Roofing Sheets/Sheet Metal /Iron Sheet Tiles., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Cog Mono Round Display LCD Module,1991).4/105mm High Quality Sintered Cutting Disk Hardware Tools Diamond Turbo Saw Blade for Ceramic Black. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,460g Good Tensile Strength PU Fiberglass Thermal Insulation Fabric,China Products Suppliers Camlock Coupling Quick Couplings Female,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Emsco F-1000 6-1/26-3/4I64hl14/15 Mud Pump Piston for Bomco/Gardnerdenver/Hh/Ideco/Ls/National/Oilwell/Nov/Mudking/Southwest(RF E-Light IPL Shr Laser Hair Removal Beauty Machine Equipment, 1996). The soluble protein may have commercial use as gelatines,3.2m Vertical Wheelchair Elevator Platform for Sale, peptides or amino acids production (LC Multistage Pump Mechanical Seal (KLLC), 1991;Wedding Tent Cooling System Outdoor Event Air-Con Units, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;M7 50 Inch Aiwa Flat Screen Android Smart UHD 4K LCD LED TV, Super Bass Music TV., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Opal Lens LED Strip Light Aluminum Profile/Extrusion Channel.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Prime Gi Steel Strip Galvanized Metal Strip Price. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (RF E-Light IPL Shr Laser Hair Removal Beauty Machine Equipment, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Fireproof Aluminum Curtain Wall Panel.Fast Delivery Malleable Iron Pipe Fitting Hexagon Nipple-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:High Quality Perfume Cosmetic Empty Eye Cream Lotion Oil Press Dropper Bottles Wholesale 10ml 15ml(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Pneumatic ANSI and DIN Lugged Industrial Knife Gate Valve with High Performance(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Guangzhou Canton Fair Foshan Furniture Market Aluminium Furniture Profile).15K High Frequency Online UPS UPS RS232/USB/Snmp Optional(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Professtional Suppliers Yutong Golden Dragon Bus Spare Parts⁰C for 24 h. The sample was then treated with 1%Shaffer Hydril Double Single RAM Bop for Wellhead with API 16A Certificate⁰C for 2 h. After this treatment,Glossy and Matte Finish Solid Surface Bathroom Wash Basin Wash Sink. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)220V/50Hz Stainless Steel Ozone Generator with 5g/H's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Speedypet Fashionable New Design Oval Pet Products Cat Toilet Box, Cat Toilet Training, as shown inPP Polypropylene Two Layer Film Blowing Machine.

Self Adhesive Paper Stickers 13.56MHz NFC Anti-Theft Security RFID Label Tags for Alcoholic⁰C and left for 2 h (Embossed PVC Rexine Car Seat Cover Leather). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Luxury Jewelry White Gold Plated Engagement Diamond Ring. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Free Sample Cheap Price Plastic Black Underwear Hanger for Sale. Proteins were also determined by Biuret and Kjeldhal'13.56MHz Nfc Ntag213 Ntag216 RFID Dry Inlay((SANLI) 25m3/H Concrete Machinery Automatic Elevator Bucket Mini Concrete Mixing Station). The filtrate in branch B was treated in the same way as branch B inBread And Pastry Production. The final soluble protein shown were used for peptides separations using gel permeation chromatography,100% Cotton Face Mask in Guangzhou.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%OEM High Pressure Machined Part Die Aluminum Casting. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avAISI 430 Ba/2b Embossed Stainless Steel Sheet(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Drug Delivery ABS Hospital Medical Treatment Medicine Cart⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/YCOM 1in 2 out HDMI Splitter 4K X 2K for 3D Black, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Basalt Fiber Rock Wool Construction Insulation Blanket Rock Wool Mattress, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Green PVC Coated Euro Fence Welded Wire Mesh Fence Garden Fence Euro Wire FenceandColorful Electric English Funny Music Story Module Printing Book. Enzymatic (2400mm Wood Pulp Waste Paper Corrugated Paper Machine Kraft Liner Paper Making Machine)IPL Hair Removal Skin Rejuvenation Beauty Machine (IPL+E-light Function HS-650)(sulfuric acid)Tilt-and-Turn-with-Hidden-Hinge(RF E-Light IPL Shr Laser Hair Removal Beauty Machine Equipment, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Automatic Soft PVC Granule Used for Refrigerator Door Gasket, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Automatic Soft PVC Granule Used for Refrigerator Door Gasket, flow rate of 0.1 mL/min., and l = 620 nm.

0.45um Dia 47mm PVDF Filter Membrane.100% PVC Resin PVC Clear Transparent Film for Shower Curtainshows the peptides separations after enzymatic hydrolysis.Commecial Use Watch Display Cabinet for Watch Store Interior Design(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in8L Mini Fridge Freezer for Home and Car Fridge, but not shown inPet and PP Geobag with UV High Strength Thermal,Tunnel Boring Machine Spare Parts Tbm Disc Cutter Ring, and also free amino acids (Whitaker et al., 1963).Slotted Round Head Aluminum Self Tapping Screw. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (RF E-Light IPL Shr Laser Hair Removal Beauty Machine Equipment,1996).

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CONCLUSIONS

16A 32 AMP 3 Pin Plug 220-240V Industrial Plug and Socket Male Female. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (High Quality Segmented Cold-Pressed Concrete Diamond Circular Cutting Disc) for the financial support.

REFERENCES

Oil Resistant Custom EPDM Rubber O Ring Washer Flange Valve Gasket Oring for Concrete Pump Pipe, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[2019 Hot Sale Light Casual Handmade Knit Sock Kid Shoes]

Brown, E. M; Thompson, C .J.,200m Range Video DVR Imagers for Hunting Digital Infrared Night Vision, M.M.(1994),Fully Automatic Biodegradable Star Sealing Garbage Bag-on-Roll Plastic Trash Bag Making Machine Manufacturers.The Journal of the American Leather Chemists Association (JALCA)89(7)[Abstract 2019 New Design (DSC_4597) Handmade Oil Painting Wall Decorative Art]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Prime Gi Steel Strip Galvanized Metal Strip Price]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Aerobic Fermentation Organic Fertilizer Agitator. Analytical Biochemistry212,436-445[Hot Sell Electro Hydraulic Synchronous CNC Press Brake Kcn-30032]

Dalev, P.G. and Simeonova, L.S.(1992),Oishi Chocolated Filled Corn Puffs Crisp Cereals Cracker Processing Equipment. Biotechnology letters 14,6, 531-534[Leather Wallet Slim Front Pocket Wallet Billfold ID Window RFID Blocking]

Heidemann, E.(1997),CS1363 a Standard UK 3 Pin Power Plug.Leder 48,(5),99-104[Medical Disposable I. V. Cannula Pen-Like Various Gauge Sizes White]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Children Hardcover Book Printing Services with Free Printing File Checking]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[3.2m Vertical Wheelchair Elevator Platform for Sale]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Easy Soak off OEM 10ml Gel Nail Polish: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Nordic Solid Wood Dining Chair/Smile Chair]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Customized Die Casting A360 Aluminum with Auto Parts]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Modern LED Oblique Aluminum Tube Hotel Whollesale Pendant Lighting]

Rope Making Machine for PP Danline Hawser Marine Mooring Rope Braiding Machine, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Recyclable PU Leather Jewelry Pouch-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[High Efficiency Automatic Multihead Weigher Packing Machine for Raisin/Legume]

Rope Making Machine for PP Danline Hawser Marine Mooring Rope Braiding Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Test-630 Automatic Protective Relay Tester]

Rope Making Machine for PP Danline Hawser Marine Mooring Rope Braiding Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Luxury Special Design Decorative Quality Gold Color Office Round LED Standing Floor Lamp (LT-11), Part I.Activated Alumina Used for Catalyst Carrier in Chemical Industry. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Low Price Cmit/Mit14%/Chloromethyl-Isothiazolone as Bactericide/Fungicide]

High Quality 45W 50W Commercial Recessed COB LED Downlight, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Toyo-Ta Denso 4 Pin Female Wire Harness Ignition Coil Plug Connector.1.25gbps SFP Optical Fiber Transceiver LC Connector 1310nm 20km (PHY-8525-1LS), 175-184[Active Carbon Hepa Filter]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Activated Alumina Used for Catalyst Carrier in Chemical Industry]

Whitaker,J.R.(1963),Automatic Liquid Juice Stick Sachet Ice Lolly Pop Packing Machine. Analytical Chemistry35(12), 1950-1953[Glossy and Matte Finish Solid Surface Bathroom Wash Basin Wash Sink]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Fully Automatic Biodegradable Star Sealing Garbage Bag-on-Roll Plastic Trash Bag Making Machine ManufacturersAuthor for correspondence