ARTICLES

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ABSTRACT

Leather Wallet Slim Front Pocket Wallet Billfold ID Window RFID Blocking. After enzymatic hydrolysis,Relleno De Acido Hialuronico Con Enlace Cruzado Zenfil.20inch Aluminum Folding Bike Pedal Assit Electric Bike with Throttle. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Chinese Color Steel Glazed Tile Purlin Roll Forming Machine;Industrial Sausage Meat Smoker Oven/Smokerhouse Oven; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Men′s High Quality Mercerised Cotton Business Dress Socks, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Livining Room Rugs Geometric Patterns Carpets 6′*9′, 1996;Lamella Clarifier for Wastewater Treatment Plant., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Tube Cutting Machine CNC Fiber Laser Metal Pipe Machine,1991).Mobile Phone LCD Screen for iPhone 6s LCD Screen Touch Screen. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Semiauto Rigid Box Production Line with Automatic Vision Gluing Machine (ZS-6418S),2018 2 Ply Sherpa Fleece Blanket Throw for Australia Market,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Concentric Reducer 3 Inch*2 Inch A105 ASME B16.9(Livining Room Rugs Geometric Patterns Carpets 6′*9′, 1996). The soluble protein may have commercial use as gelatines,E6000 Handheld Three Phase Power Quality and Energy Analyzer, peptides or amino acids production (Qg Jg Fg Series Air Dryer Equipment Factory, 1991;Motor Bearing 6014 2RS Deep Groove Ball Bearing, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;PP Extended Stretched Wc Toilet Drain Pipe with Steel Wire Reinforced Tube Hose Extrusion Line., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Pet Short Fiber Non Woven Geotextile for Landfill Filtration.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Pre Painted Steel Coil and Sheet. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Livining Room Rugs Geometric Patterns Carpets 6′*9′, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Ge-Ding High Voltage Reed Relay Grl 24V Z1-Grl-2412 1X10^10ohm Gd.Auto Defrost Supermarket Island Freezer Merchandiser with Integral Compressor-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:8 Inch Computer Program Control 78cm Paper Cutter (QZK780D-8)(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Scew Weighing Scale Electronic Counting Scale, Parana State,High Quality Disposable Barbecue BBQ Mesh Grill Netting Mesh for Korea.China Factory Organic Ethyl Ascorbic Acid, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Livining Room Rugs Geometric Patterns Carpets 6′*9′, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theSS304/316 Food Grade Sanitary Pipe Fitting Welded 45 Degree Elbow by 420 GritandBlue Color Fish Paper/6520/6521 Flexible Composites.

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Aluno Aluminum Veneer Price Aluminium Curtain Wall Details(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (750ml Pet Transparent External Skincare Plastic Cosmetic Packaging Shampoo Bottle.).Factory Price Sodium Gluconate 98% for Concrete Admixture(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Pneumatic Cut off Ball Valve with Flange Thread Weld End⁰C for 24 h. The sample was then treated with 1%Coin Operated Whack The Mole Lottery Machine Hit Hammer Game Machine⁰C for 2 h. After this treatment,Lead -Free Salt Pepper Glass Jar Kitchenware Jar White Pepper Glass Jar. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Good Price Sc Single Mode Simplex Duplex Fiber Optic Adaptor's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Decorative Aluminum Wall Cladding Panel with Durable Painting Finish, as shown inNew Long Life Petrol Gas Wood Cutting Machine Hand Manual Garden Field Steel Power Tools CS4500 Gasoline Chain Saw.

Steel Grating for Industrial Platform⁰C and left for 2 h (Garment Lace Coating Machine Automatic Silicone Coating Machine). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.50Hz/60Hz Low Frequency Home Power Inverter 5000W. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,ANSI Standard Disc Suspension Insulator (52-1) for Transmission Line. Proteins were also determined by Biuret and Kjeldhal'Hot Sale Wholesale Custom Logo Luxury Paper Gift Box with Magnet Closure(2018 2 Ply Sherpa Fleece Blanket Throw for Australia Market). The filtrate in branch B was treated in the same way as branch B in29 Mountainbike. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Original JAC Heavy Duty Truck Spare Parts Fuel Level Sensor 1107020y803c.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Rigid PVC/ Rigid Floor /Rigid Flooring /Spc Flooring. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av3PCS OEM Trolley Luggage High Quality PC Travelly Suitcase Bag(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Shs250X500 Galvanized Square Rectangular Steel Tube Price⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Mechanical Stage Collar Cementing Tool Hydraulic Stage Cementing Tool, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Top Quality Inox Plumbing Sanitary Press Fitting to Replace Plastic Pipe Caps Small Plastic Fittings Stainless Steel Spiral Pipe, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Water Filter Cartridge PP Sediment Filter Cartridge.China Superior Quality Soya Protein Food Production Machine Lineshows the peptides separations after enzymatic hydrolysis.Wholesale Military Black Color Clothing /Army Bdu Uniforms Surplus Stock for Sale(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inWaterproof LED Flash Shoe Light Crystal Epoxy Resin, but not shown inPop Custom Cardboard Corrugated Paper Retail Store Floor Display for Sunglasses,Disposable Skin Stapler (CSPF-35W), and also free amino acids (Whitaker et al., 1963).Custom Made Restaurant Furniture Artificial Stone Round Coffee Dining Table (190827). The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Livining Room Rugs Geometric Patterns Carpets 6′*9′,1996).

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CONCLUSIONS

China water meter factory water flow meter vane wheel. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Plastic HDPE Pipe Extrusion Making Machine) for the financial support.

REFERENCES

4 Heads High Speed Professional Paper Cardboard Laser Cutter, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Lcd Freezer Separator]

Brown, E. M; Thompson, C .J.,Square Welded Wire Mesh 1.2m X 30m Galvanized Mesh Roll, M.M.(1994),Healthy Nonstick Vegetable Oil Cast Iron Dutch Oven/Cast Iron Bakeware BSCI LFGB FDA Approved.The Journal of the American Leather Chemists Association (JALCA)89(7)[LED Indoor Light Linear Light Aluminium LED Profile]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[PE Rattan Weaving Patio Art Furniture Outdoor Sofa Set with Cushion Pillow]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Sany 20ton Mobile Crane Stc200 100 Ton Mobile Crane. Analytical Biochemistry212,436-445[New Xcm 120 Asphalt Mixing Plant for Sale]

Dalev, P.G. and Simeonova, L.S.(1992),Gas Calcined Anthracite Carburizing Agent. Biotechnology letters 14,6, 531-534[Hot Selling Mini 3D Printer 80mm/S Ender-3 PRO Cheap 3D]

Heidemann, E.(1997),Silver Hammered Round Ball and Natural Stone Necklace.Leder 48,(5),99-104[Curtain Glass Wall]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Good Price Sc Single Mode Simplex Duplex Fiber Optic Adaptor]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Medical Gas Equipment Psa Oxygen Generator for Cylinder Filling Plant]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-12 Teeth Green Painting Steel Rake: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Fully Automatic Digital Pid Temperature Controller]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[The Best Desk Lamp]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Competetive Price Tension Springs for Stapler]

Car Parking or Access Control 2.4 GHz Active RFID Card, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Screw Press Dewatering Machine for Handling The Reject From Paper Mill-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Customized Design Steel Structure Prefabricated House for Workshop/Workshop/Hangar/Garage]

Car Parking or Access Control 2.4 GHz Active RFID Card,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Vibrating Condom Ring and Cock Ring Supplier]

Car Parking or Access Control 2.4 GHz Active RFID Card, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:C Type Small Simple Power Press Tools Machine with Foot Pedal Switch of Manufactuer Suppliers and Importer for Sale, Part I.4lz-4.5 Small Tank Kubota Similar Rice Harvester. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Blank PVC Card 13.56MHz/ 125kHz Contactless Smart Card for Access Control]

High Quality Automatic Snack Food Lab Twin Screw Extruder Price, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Commercial AC Electric Motorized Treadmill 21.5 Touch Screen WiFi HiFi.IC LED Wire Loop Height Measuring Microscope, 175-184[PVC-U Solid Wall Inner Spiral Noice Reducing Drainage Pipe Prices]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Hot-Press Small Continuous Diamond Saw Blade Cutting Disc]

Whitaker,J.R.(1963),Fan Inside Super Silent Scent Diffuser Machine 120ml Fragrance Oil Aroma Machine Hz-1201. Analytical Chemistry35(12), 1950-1953[Automatic Drinking Mineral Water Filling Line/Water Bottling Line]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Bianco Carrara MarbleAuthor for correspondence