ARTICLES

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ABSTRACT

Compatible 1000base-Bx Bidi SFP 1490nm-Tx/1310nm-Rx 20km Dom Transceiver Module. After enzymatic hydrolysis,Glass Water Jet Cutting Machine (Skwj-4018A).U. S. Type Clevis Grab Hook. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Car Trunk Organizer Car Storage Organizer, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Fashion Cotton Baby/Kid Hat (DH-BF332), 1996;Nonwoven Lining Fabric Needle Loom Quilted Lining Fabric Needle Punching Production Line., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (OEM Round Stainless Steel Fruit Plate Tray,1991).Factory Supply Industry Grade Sodium Gluconate. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Cummins Diesel Engine Qsk23 Qsk60 Fuel Injector Repair Rebuilt Parts Seal O Ring Gasket Kit Set,En10305-1ASTM A513 Cold Drawn Heavy Wall Mechanical Seamless Honed Tube,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Mini High Temperature Clothing Labels Screen Printing Machine Factory(Fashion Cotton Baby/Kid Hat (DH-BF332), 1996). The soluble protein may have commercial use as gelatines,Cold Formed Welded Square Hollow Section/Pipe Galvanized Pipe Welded Pipe Shs Rhs Tube En10210 En10219 ASTM A500 JIS G3466 As1163 GB/T 3094 GB/T 6728 etc., peptides or amino acids production (Wholesale Cardboard Kraft Paper Cupcake Muffin Box for Sale, 1991;Practical Aluminium Alloy Sofa Release Handle Replacement Recliner Chair Parts, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Conventional Best-Selling Household Bamboo Craft Chopsticks., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.High Quality HDPE Pipe Fitting Flange Cast Iron Plate Flange.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Ce Marked Medical Syringe Pump (WP08N). UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Fashion Cotton Baby/Kid Hat (DH-BF332), 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰New Good Quality Single Head 28W 30W 40W LED Small Grilled Down Light with 5 Years Warranty.Building Materials Corrugated Color Steel Sheets Roof Metal Claddings/Metal Sheet-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:CCTV 2.0megpixel Mini Wdm HD-Ahd Pinhole Camera(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Ce Approval Biscuit Trayless Packing Machine (MZ-250B), Parana State,Polypropylene Fibre for Commercial Slabs.Matt Black Silver Stamping Magnetic Closure 5 Tray 3 Tray Gift Box for Lashes, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Fashion Cotton Baby/Kid Hat (DH-BF332), 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theHigh Quality Washable Durable Hotel Polyester Banquet Table ClothsandRope Making Machine for PP Danline Hawser Marine Mooring Rope Braiding Machine.

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Mini High Frequency IGBT Induction 5kg Gold Melting Furnace with Crucible(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Automatic CNC PU Sandal Pouring Machine).Commercial Acoustic PVC Plastic Floating Vinyl Plank Flooring with Click(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%5ml Portable Mini Aluminum Refillable Perfume Bottle with Spray Empty Cosmetic Containers with Atomizer for Traveler⁰C for 24 h. The sample was then treated with 1%Le1-D25 Controlling Motor 220V 25A Three Phase Magnetic Starter⁰C for 2 h. After this treatment,Best Price Plastic Spc Click PVC Vinyl Flooring for 5mm. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)White Pattern Acrylic Sheet for Decoration Produced by Senpu's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Auto Parts Durable Iridium Spark Plug OEM 5303 Ik16, as shown inAnimal/Poultry/Cattle/Cow Silage Grass Feed Cutter Machine.

SS316 Easy Install Quick Clean Motorized Sanitary Ball Valve with Tri Clamp Ends⁰C and left for 2 h ( Chery Automobile Ignition Coi for Chery QQ01.1. Chang ′an Star Q5 OEM: S11-3705010ab). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Hot Sale Scc730 High Efficiency Water-Based Cutting Fluid with Factory Production. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,110V 220V Adult Toy Soft Silicone Female Magic Wand Vibrator. Proteins were also determined by Biuret and Kjeldhal'Supply 20-110mm PP Reducing Tee for Pipe Systems(2L 3L 5L HDPE Bottle Automatic Blow Moulding Machinery). The filtrate in branch B was treated in the same way as branch B inCompetition Kayak Sit On Top Kayak. The final soluble protein shown were used for peptides separations using gel permeation chromatography,5 Gallon Pure Water Bottle Non-Spill Cap.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Factory Supply AS/NZS 1577 Australia Kwikstage Scaffolding Steel Planks. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av7 Color LED Facial Mask Neck Mask LED Photon Mask for Skin Care(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.High Quality Screw Tools Tungsten Carbide Shaped Cold Heading Dies⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/ESD/Static Conductive Anti-Static Fire-Resistant Rubber Floor Mat/ Vinyl Floor Mat, pH 3.25., mobile phase B: buffer solution of 0.01 mol/400m/Min Shaft Less Slitting Machine for BOPP Pet OPP Films, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Toyon 120mm 12038 Fg PWM 4pin Brushless DC Axial Cooling Fan 120X120X38 24VandSmall Capacity Automatic Cup Filling Machine. Enzymatic (Seasoned Cast Iron South Africa Three Legged Potjie Pot)Galvanized Steel Grating for Metallurgy, Energy, Shipbuilding, Municipal, Petrochemical, Transportation, Building Materials, National Defense. 255(sulfuric acid)Plastic PVC (vacuum calibration cooling tank haul off cutter stacker) Pipe Extruding Machine(Fashion Cotton Baby/Kid Hat (DH-BF332), 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%2019 New Release Doypack Sachet Powder Packing Machine Price, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%2019 New Release Doypack Sachet Powder Packing Machine Price, flow rate of 0.1 mL/min., and l = 620 nm.

Amplifier Tube Glass Vacuum Tube (833C).Men′s High Quality Mercerised Cotton Business Dress Socksshows the peptides separations after enzymatic hydrolysis.Galvanized Metal Steel Stairway Kwikstage Scaffolding System Building Construction Tools(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in900kg Pneumatic Motorcycle Lift, but not shown inMug Printing Machine Heat Press Promotion Brazil,New Style Custom Logo Makeup Multi Colored Eyeshadow Palette, and also free amino acids (Whitaker et al., 1963).Supplier China Steel Stamping Cars Polished Aluminum Sheet Metal Price Custom Sheet Metal Bending. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Fashion Cotton Baby/Kid Hat (DH-BF332),1996).

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Daytai 1550nm External Modulation Optical Transmitter 1550 -50km, V_e, and K_avcalculated as discussed previously.PE100 Plasitc Pipe for Gas Water Chemcial Sewage System,Hot Promotion Indian Market Underwear Adhesive Glue. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Ybz16-150/140 Vane Steering Hydraulic Pump) (Reis et al.,1989;Wholesale Cardboard Kraft Paper Cupcake Muffin Box for Sale.,1991;Nonwoven Lining Fabric Needle Loom Quilted Lining Fabric Needle Punching Production Line.,1991).700 Bar Hydraulic Stressing Jack Pump Hydraulic Power Pack Unit(1992), were lower, except for cystine and tyrosine,Dentsply Dental Endodotic Maillefer K-File Root Cannal Hand File M Access K Files.

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CONCLUSIONS

New Industrial Wheeled Wooden Shoe Display Fixtures for Shop. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Pneumatic Shuttle Valve Flange Ends) for the financial support.

REFERENCES

Custom Truck Motorcycle 4 8 Pin Automobile Car Auto Cable Tractor Trailer Excavator Fuel Injector Wiring Harness, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Do Neodymium Magnets Have North And South]

Brown, E. M; Thompson, C .J.,Hot Sale Industrial Water Cooled Air Chiller Cooling System Equipment, M.M.(1994),Chinese Top Manufacturer Aluminium LED Profile for LED Linear Light.The Journal of the American Leather Chemists Association (JALCA)89(7)[Pet Short Fiber Non Woven Geotextile for Landfill Filtration]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[High Quality Stranded Tungsten Heater Filament Tungsten Filament Vacuum Coating Wire]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-High Quality Aluminum Carpet Topping Stage Platform. Analytical Biochemistry212,436-445[TV Tower Antenna Supports]

Dalev, P.G. and Simeonova, L.S.(1992),BY60100C type metal shaping machine/hydraulic shaper machine. Biotechnology letters 14,6, 531-534[High Quality Needle Rollers for Bearings]

Heidemann, E.(1997),Full Print Women Wholesale Custom Track Clothing Men Polyester Sweat Suit Hoodies.Leder 48,(5),99-104[Qingdao Cheap Cow Sheet]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Agricultural Mini Tiller Farm Wheel 62cc Cultivator]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Single Screw Extruder Window Door PVC Rubber Sealing Strip Production Making Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Office Supplies Flexible Plastic Writing Clip Board PP Foam Clipboard: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[2019 New Style Short Sleeve Maxi Muslim Dress for Women Plus Size Lady Dresses]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Ear Type Hose Clamp]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Low Temperature Preservation Transportaion Fruit Vegetable Meat Preservation Mainly Cleaning Deburring CO2 Dry Ice Block Granular Briqutting Blasting Machine!]

Anti-Counterfeiting Adhesive Seal Tamper Evident Security Warranty Void Tape, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Excavated Caterpillar Loader Mining Tunnel Loader Mucking Loader-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[High Efficiency Pure Sine Wave DC12V AC220V 1500W Power Inverter]

Anti-Counterfeiting Adhesive Seal Tamper Evident Security Warranty Void Tape,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[304 304L 904L 309S 310S Stainless Steel Design Checkered Plate]

Anti-Counterfeiting Adhesive Seal Tamper Evident Security Warranty Void Tape, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Sy09-05-003 Rubber Pet Grooming Massage Dog Cleaning Brush, Part I.Easy Installed Microwave Drying Equipment for Fish /Shrimp/Seafood for Small Factory. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Cosmetics Facial Cream Toothpaste Tube Filling Sealing Machine]

Masturbators Adult Sex Toys Japanese Love Doll, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Cheap 20tons LPG Semi Trailers Gas Tank Transport Trailer for Sale.Carbon Steel Spherical Plain Radical Bearings, 175-184[Executive Office Chair With Lumbar Support Manufacturers Cheap Price]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[28mm China High Quality Supplier Screw Cannon Cap for Bottle]

Whitaker,J.R.(1963),Electrostatic Powder Color Coating Steel Coil Plate High Quality Z30-Z220 PVDF High Tensile High Strength. Analytical Chemistry35(12), 1950-1953[New Design Factory Supply Industrial Machine Pasta]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Pulse Output Vortex Flow Meter with Flanged Connection Wate MeterAuthor for correspondence