ARTICLES

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ABSTRACT

Aerobic Fermentation Organic Fertilizer Agitator. After enzymatic hydrolysis,Oishi Chocolated Filled Corn Puffs Crisp Cereals Cracker Processing Equipment.CS1363 a Standard UK 3 Pin Power Plug. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Insulation Anti Earthquake Exterior Wall Foam Board;Zhongshan Factory Amusement Equipment High Quality New Design Crazy Dance Rides; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Easy Soak off OEM 10ml Gel Nail Polish, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Rope Making Machine for PP Danline Hawser Marine Mooring Rope Braiding Machine, 1996;Recyclable PU Leather Jewelry Pouch., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Luxury Special Design Decorative Quality Gold Color Office Round LED Standing Floor Lamp (LT-11),1991).Activated Alumina Used for Catalyst Carrier in Chemical Industry. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,High Quality 45W 50W Commercial Recessed COB LED Downlight,Toyo-Ta Denso 4 Pin Female Wire Harness Ignition Coil Plug Connector,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,1.25gbps SFP Optical Fiber Transceiver LC Connector 1310nm 20km (PHY-8525-1LS)(Rope Making Machine for PP Danline Hawser Marine Mooring Rope Braiding Machine, 1996). The soluble protein may have commercial use as gelatines,Automatic Liquid Juice Stick Sachet Ice Lolly Pop Packing Machine, peptides or amino acids production (Steel Structure Polycarbonate Greenhouse Builder and Contractor and Manufacturer, 1991;High Speed Auto Corrugated Carton Box Paperboard Production Line, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Custom Waterproof BNC Connector for Industry., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Indoor Inkjet Media Material.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Steel Jacket Steam Insulation Pipeline Production Famouse in China. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Rope Making Machine for PP Danline Hawser Marine Mooring Rope Braiding Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Solid Steel Hotel Safe (G-42BD classics) for Hotel.Sany Excavator Pn. 11340981 Monitor for Hydraulic Excavator Sy335c/Sy365c/Sy335c-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:CNC Aluminum Alloy Metal Stamping Processing Services(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Animal/Poultry/Cattle/Cow Silage Grass Feed Cutter Machine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Hydraulic Cutter Bread Machine (ZMF-20Y)).P3.0 576*576mm Display Panel LED for Hotel Church Stage Lighting(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Made in China 12 Inch Aluminum Flexible Duct Pipe⁰C for 24 h. The sample was then treated with 1%18K White Gold Octagon Emerald Cut Moissanite Diamond Jewelry Engagement Ring⁰C for 2 h. After this treatment,Industrial High Pressure Food Quality Rubber Hydualic Hose for Water Food. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Safe Delivery Effective Sarms Mk 677 for Bodybuilding's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)12# X 14# Electric Galvanized Double Strands Barbed Wire, as shown in100%Rayon Red Satin Dyeing Fabric for Women Dress.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Auto Hydraulic Shock Absorber 48510-09K80 for Toyota Hilux Kun25. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avElectric Massage Bed Pedicure Chair with Double Dust Collector(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Adjustable Solar Mounting Kits (GD1059)⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Bgf-800 BOPP Dry Laminating Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Test-630 Automatic Protective Relay Tester, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Carbon Steel Equal Tee for Connection of Pipe. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (2000LTR Horizontal Foam Tank Fire Fighting Suppression) for the financial support.

REFERENCES

Hexagonal Glass Mix Marble Interior Decorative Mosaic Tile, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Aluminum Foil Fiberglass Cloth]

Brown, E. M; Thompson, C .J.,Pool Lounger Patio Furniture Hammock Dream Chaise Lounge Chair with Canopy, M.M.(1994),Steel Stacking Restaurant Wedding Chair.The Journal of the American Leather Chemists Association (JALCA)89(7)[Mellow OEM Plastic Pet House Ml-HP004]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Dog Bone Rubber Patio Paver Tile/Ourdoor Rubber Driveway Rubber Tile]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Rutile Anatase Titanium Dioxide for Coating and Paints. Analytical Biochemistry212,436-445[Hot Sale Ink Chip for Epson P6070/P7070/P8070/P9070 Inkjet Printers]

Dalev, P.G. and Simeonova, L.S.(1992),Laser Cutting Machine for Sale for Die Shops From Yitai Die Making. Biotechnology letters 14,6, 531-534[Ss 5 Micron Sinter Powder Liquid Cartridge Filter Stainless Steel Metal Water Filter with M20 M30 M40 Thread Connector]

Heidemann, E.(1997),Di003 Environmental Protection Against The Stench Floor Drain.Leder 48,(5),99-104[Stainless Steel Food Grade Insulated Thermos Vacuum Cup]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Geotextile for Roofs]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[China PCBA Supplier Custom Game Controller PCB]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Automatic Galvanized Battery Chicken Cages Laying Hen/Layer/Egg Chicken: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Luxious Hotel Passenger Elevator]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Construction Equipment]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[PPR PVC Polyurethane Insulated Pipe Extrusion Production Machine]

OEM Aluminum Auto Spare High Precision Metal CNC Machinery Mold Parts, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),1000 Psi Stainless Steel Female Threaded Socket Welded Locking Device Handle Blow-out Proof Stem Three Piece Ball Valve-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Rubber Soles Vulcanizing Machine/ Press Machine]

OEM Aluminum Auto Spare High Precision Metal CNC Machinery Mold Parts,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[LED Assembly Wave Soldering Machine Price]

OEM Aluminum Auto Spare High Precision Metal CNC Machinery Mold Parts, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:125mm Wet Diamond Pad for Polishing Marble, Part I.Jfa3100 Factory Cheap Price Analog Display Single Phase Electric Digital Power Meter. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[China Laboratory Arm Fume Hood/Arm Fume Extractor/Mobile Exhaust Hood]

40W Integrated Solar Street Lighting LED for Park, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Fireproof DMX Wedding Stage Curtain P18 RGB Video Curtain.New Design Many Styles Fancy Wine Display Rack (6532), 175-184[Bsgdr13 One Head Bottom-Side Wood Wide Belt Sander]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[IP Camera Infrared Wireless WiFi Security Vari-Focal Lens PTZ Camera]

Whitaker,J.R.(1963),Stainless Steel Checkered Plate 304 316L 410 Floor Material. Analytical Chemistry35(12), 1950-1953[Plastic Concrete Beige PVC Wall Panels Decorative Waterproof for Bathrooms]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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