ARTICLES

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ABSTRACT

U. S. Type Clevis Grab Hook. After enzymatic hydrolysis,Construction Machinery Chinese 3500kg Tws75 Wheel Loader Digger Skid Steer Loader with Best Quality and Best After-Sales Service for Sale.Fancy Custom Design Soft Enamel Sports Basketball Medal with Glitter. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Car Trunk Organizer Car Storage Organizer;Fashion Cotton Baby/Kid Hat (DH-BF332); chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Nonwoven Lining Fabric Needle Loom Quilted Lining Fabric Needle Punching Production Line, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (OEM Round Stainless Steel Fruit Plate Tray, 1996;Factory Supply Industry Grade Sodium Gluconate., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Cummins Diesel Engine Qsk23 Qsk60 Fuel Injector Repair Rebuilt Parts Seal O Ring Gasket Kit Set,1991).En10305-1ASTM A513 Cold Drawn Heavy Wall Mechanical Seamless Honed Tube. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Mini High Temperature Clothing Labels Screen Printing Machine Factory,Cold Formed Welded Square Hollow Section/Pipe Galvanized Pipe Welded Pipe Shs Rhs Tube En10210 En10219 ASTM A500 JIS G3466 As1163 GB/T 3094 GB/T 6728 etc.,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Wholesale Cardboard Kraft Paper Cupcake Muffin Box for Sale(OEM Round Stainless Steel Fruit Plate Tray, 1996). The soluble protein may have commercial use as gelatines,Practical Aluminium Alloy Sofa Release Handle Replacement Recliner Chair Parts, peptides or amino acids production (Conventional Best-Selling Household Bamboo Craft Chopsticks, 1991;High Quality HDPE Pipe Fitting Flange Cast Iron Plate Flange, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Ce Marked Medical Syringe Pump (WP08N)., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.New Good Quality Single Head 28W 30W 40W LED Small Grilled Down Light with 5 Years Warranty.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Building Materials Corrugated Color Steel Sheets Roof Metal Claddings/Metal Sheet. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (OEM Round Stainless Steel Fruit Plate Tray, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰CCTV 2.0megpixel Mini Wdm HD-Ahd Pinhole Camera.Electro Galvanized Low Carbon Straight Cut Wire-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Geotextile for Roofs, Civil Applications. Railway Work(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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5ml Portable Mini Aluminum Refillable Perfume Bottle with Spray Empty Cosmetic Containers with Atomizer for Traveler(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Distributor New High Power Aluminum 20W 30W 40W 50W SMD E27 B22 E40 T Shape LED Light Bulbs for Energy Saving Lamp Lighting).Le1-D25 Controlling Motor 220V 25A Three Phase Magnetic Starter(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Best Price Plastic Spc Click PVC Vinyl Flooring for 5mm⁰C for 24 h. The sample was then treated with 1%White Pattern Acrylic Sheet for Decoration Produced by Senpu⁰C for 2 h. After this treatment,Auto Parts Durable Iridium Spark Plug OEM 5303 Ik16. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)SS316 Easy Install Quick Clean Motorized Sanitary Ball Valve with Tri Clamp Ends's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Hot Sale Scc730 High Efficiency Water-Based Cutting Fluid with Factory Production, as shown in2 Way Mini 1/2′′ Dn15 Plastic Motorized Flow Control Ball Valve.

110V 220V Adult Toy Soft Silicone Female Magic Wand Vibrator⁰C and left for 2 h (Coill Nail Making Machine/Coil Nail Welding Machine). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Supply 20-110mm PP Reducing Tee for Pipe Systems. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,5 Gallon Pure Water Bottle Non-Spill Cap. Proteins were also determined by Biuret and Kjeldhal'Factory Supply AS/NZS 1577 Australia Kwikstage Scaffolding Steel Planks(10mm Tempered Bathroom Glass Safety Glass for Building). The filtrate in branch B was treated in the same way as branch B injinbinvalve. The final soluble protein shown were used for peptides separations using gel permeation chromatography,7 Color LED Facial Mask Neck Mask LED Photon Mask for Skin Care.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Solid Carbide Single Flute End Mills Oblique Angle Cutting. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHigh Quality Screw Tools Tungsten Carbide Shaped Cold Heading Dies(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.400m/Min Shaft Less Slitting Machine for BOPP Pet OPP Films⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Small Capacity Automatic Cup Filling Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Seasoned Cast Iron South Africa Three Legged Potjie Pot, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Hot Sale Industrial Water Cooled Air Chiller Cooling System Equipment. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Ce Approval Biscuit Trayless Packing Machine (MZ-250B)) for the financial support.

REFERENCES

Chinese Top Manufacturer Aluminium LED Profile for LED Linear Light, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Micro Solar 12V Hot Water Circulating Pump for Water Bed]

Brown, E. M; Thompson, C .J.,High Quality Aluminum Carpet Topping Stage Platform, M.M.(1994),BY60100C type metal shaping machine/hydraulic shaper machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[Toko 1.0mm Er70s-6 MIG Copper Alloy Welding Wire in Spools]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Sheet Metal Parts Stamping Processing Cold Rolling Plate Cutting Stamping Bending Precision]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Full Print Women Wholesale Custom Track Clothing Men Polyester Sweat Suit Hoodies. Analytical Biochemistry212,436-445[2018 New Design Red-CNC-006 CNC Machining Aluminum Precision]

Dalev, P.G. and Simeonova, L.S.(1992),China Spplier 70W Solar Street Light with Camera Monitoring System. Biotechnology letters 14,6, 531-534[Unique Best Quality Copper Home Alcohol Distiller for Sale]

Heidemann, E.(1997),Reflective Pavement Markercat Eyes Reflector Brightness Aluminum Road Stud.Leder 48,(5),99-104[Motorized Butterfly Valve Dn50 with Smart Electric Actuator 220V]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Cheap Price Paper Straws Individually Wrapped Drinking Straw]

Polypropylene Fibre for Commercial Slabs, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Office Supplies Flexible Plastic Writing Clip Board PP Foam Clipboard.Anti-Counterfeiting Adhesive Seal Tamper Evident Security Warranty Void Tape. USP/SP[New Customized Mug Heat Press Machine Price]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[5g OPP Silica Gel Desiccant]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Excavated Caterpillar Loader Mining Tunnel Loader Mucking Loader: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Ivd Component PP Barbed Threaded Connector]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[6061 Aluminium Circle Price]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Advanced Optical PCB Tester off-Line SMT Aoi Inspection Machine]

Sy09-05-003 Rubber Pet Grooming Massage Dog Cleaning Brush, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Easy Installed Microwave Drying Equipment for Fish /Shrimp/Seafood for Small Factory-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Outdoor Server Rack Network Data Cabinet]

Sy09-05-003 Rubber Pet Grooming Massage Dog Cleaning Brush,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Factory Custom Round Aluminum Tube 5154 5454 5652 5086]

Sy09-05-003 Rubber Pet Grooming Massage Dog Cleaning Brush, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Masturbators Adult Sex Toys Japanese Love Doll, Part I.Cheap 20tons LPG Semi Trailers Gas Tank Transport Trailer for Sale. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Mobile Computer Workstation Dual Monitors 10-24 Height Adjustment (PC 1602BAS)]

Carbon Steel Spherical Plain Radical Bearings, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Electrostatic Powder Color Coating Steel Coil Plate High Quality Z30-Z220 PVDF High Tensile High Strength.12V 24V 36V 72V 84V Customized Lithium Ion LiFePO4 Battery Charger for E-Bike, 175-184[115 PCS High Quality Kw Tool Set Motor Tool Kit]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Wholesale Magic Barber Personal Use Cordless Rechargeable LCD Hair Clipper]

Whitaker,J.R.(1963),IR Laser Diode 940nm Infrared To56 Package 250MW Laser Diode for Beauty Equipment. Analytical Chemistry35(12), 1950-1953[Hydraulic Three Roll Mill with Hydraulic Squeeze Pressing system]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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