ARTICLES

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ABSTRACT

Home Design Solid Wood Double Adult Bed Bedroom Furniture. After enzymatic hydrolysis,Epon ONU with 4ge 2VoIP Pots 2.4G 5g Twon Antenna WiFi Gepon Ont.High Quality Dental Primary Molar Crown Stainless Steel Kids Crowns. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;304 Precision Professional Dimpled Stainless Steel Plate;Boundry Wall Powder Coated Galvanized Welded Double Wire Mesh Fence; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Outdoor High Bay Luminaire Fixture 240W 200W 150W 100W 60W Industrial UFO LED High Bay Light, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Stainless Steel Vertical Pipe Clamp Cast Iron Machinery Joint Flexible Shaft Coupling, 1996;Prefabricated Steel Framed Sheds to Metro, Railway Station, Underground Carport, Airport., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Special Shape Spring with Good Quality and Fast Delivery,1991).Better Quality White Coated Fbb Paper Ivory Board. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Excellent Plastic Extruder in Pet PP Packing Strap Machine,Food Zip Lock Square Bottom Brown Paper Coffee Bag Supplier From China,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,China Factory Supplying High Purity Magnesium Alloy Ingot 99.0%~99.9%(Stainless Steel Vertical Pipe Clamp Cast Iron Machinery Joint Flexible Shaft Coupling, 1996). The soluble protein may have commercial use as gelatines,Mirror Aluminum Reflective Sheet for Lighting 1050/1060/1070/1100/1200, peptides or amino acids production (2019 New Advertising Inflatable LED Jellyfish Balloon for Sale, 1991;Tree Root Grinder Stump Grinder, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Open Narrow Slot Kx Electrical Wire Cable Raceway Ducts/Yellow Jacket Cable Protector., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Customization! Bdy380CD Horizontal Directional Drilling Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-V Folding HDPE LDPE Coreless Degradable Star Sealing Corn Starch Trash Coreless Rolling Garbage Flat Plastic Bag Making Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Stainless Steel Vertical Pipe Clamp Cast Iron Machinery Joint Flexible Shaft Coupling, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Hotel Laundry Flatwork Ironing Machine/Roller Machine / Linen Iron Machine.6D Galvanic Facial Machine Eye Lifting Skin Care Beauty Equipment-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Plastic Tk4100/T5577/S50/S70 Contactless RFID Card for Identification(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Max Twintier Rb441t Using Wire Coils Tw1061t(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (New Vmc Machine Auto Parts Processing CNC Vertical Machining Centre).Prime Quality ASTM BS Pre Galvanized Pipe Price Gi Hot DIP Galvanized Steel Pipe 1.5‘ ’ 1mm(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Manufacturer Supply Durable Grey Color Non-Slip EVA Mat⁰C for 24 h. The sample was then treated with 1%Feed Storage OEM Grain Storage Silo 1cbm to 200cbm⁰C for 2 h. After this treatment,Candy Colorful Bentgo All-in-One Stackable Lunch/Bento Box. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Captain Woodwork Sanding Machine/Sanding Table's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Ce/FM/UL Approved Ductile Iron Grooved Flexible Reducing Coupling, as shown inCustomized Factory Supply Stainless Steel T Shape Bolt.

Atg39 100W LED Flood Light Wall Light Wall Washer⁰C and left for 2 h (UHF EPC G2 RFID Label 1 860-960MHz Rolls RFID Sticker Tag). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.China Manufacturer Premium Outdoor Use Climbing  Plant  Support  Net. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Gold Inlaid 24K Gold / Flowers. Proteins were also determined by Biuret and Kjeldhal'22 Inch Garment Bag Crospack Suit Travel Bag with Shoulder Strap(Creative New Product Coin Holder Keychain). The filtrate in branch B was treated in the same way as branch B inCable Terminal Ends. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Permanent Skewed Segment Neodymium NdFeB Magnets with Different Customized Shapes.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Yashi 65inch Infrared Touch Whiteboard Interactive Smart Board. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHydraulic Cutter Bread Machine (ZMF-20Y)(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Yet905 Wireless Keypad Doorbell Kits with Remote Control⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Hotel Safe with Digital Keypad and Mechanical Key (ELE-SA200FR), pH 3.25., mobile phase B: buffer solution of 0.01 mol/Original Sinotruk HOWO Truck Parts - Exhaust Pipe Wg9725542042, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Hsc Heavy Duty 5 Ton Chain Pulley BlockandChina Factory Queen Mattress with Visco Memory Foam and Latex. Enzymatic (Multi-Port Ss 304 Manual Three Way Non-Retention Ball Valve)Convenient Operation Hard-Wearing Smooth Feeding Biodegradable Bagasse Pulp Tableware Plate Making Machine/Former/Maker(sulfuric acid)HD Night Vision IR Laser Camera with PTZ for 1200m Surveillance(Stainless Steel Vertical Pipe Clamp Cast Iron Machinery Joint Flexible Shaft Coupling, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Ceiling Mounted IV Stand Stainlesss Steel I V Pole, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Ceiling Mounted IV Stand Stainlesss Steel I V Pole, flow rate of 0.1 mL/min., and l = 620 nm.

Pipe Connection Types of Pressing Scaffolding Universal Clamp.14 Heads Double Door Multihead Weighershows the peptides separations after enzymatic hydrolysis.Ml33-2mm Eddy Current Linear Position/Displacement Sensor(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inGlass Water Jet Cutting Machine (Skwj-4018A), but not shown inAutomatic CNC Centerless Lathe for Peeling Steel Round Bar,Veterinarian Ultrasound Diagnostic System for Cow, Horse, Swine, Goat, and also free amino acids (Whitaker et al., 1963).Wholesale 25kg 50kg White PP Woven Sack for Flour Feed Grain. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Stainless Steel Vertical Pipe Clamp Cast Iron Machinery Joint Flexible Shaft Coupling,1996).

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CONCLUSIONS

UV Resistant Durable HDPE Reinforced Geomembrane HDPE. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (FKM Bearing Oil Seal /Customized) for the financial support.

REFERENCES

2070 4th Axis CNC, Atc CNC Router Machine, Carving Engraving Machinery with High Z Axis, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[GN: Glass Fiber & Nano Fiber Membrane Filter Cartridge for Inks]

Brown, E. M; Thompson, C .J.,Cheap Price Made in China Disposable Medical Heat Seal Flat Sterilization Pouch, M.M.(1994),PE Film Blowing Machine (SJ-1000-1300-1500).The Journal of the American Leather Chemists Association (JALCA)89(7)[OEM High Pressure Machined Part Die Aluminum Casting]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[OEM Precision Aluminum Alloy/Zinc Alloy Die Casting]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Eco-Friendly Summer Beach Lady Handmade Paper Shopping Straw Basket Bag. Analytical Biochemistry212,436-445[Scantech Handheld Hscan331 3D Laser Scanner for Quality Inspection]

Dalev, P.G. and Simeonova, L.S.(1992),304 304L 904L 309S 310S Stainless Steel Design Checkered Plate. Biotechnology letters 14,6, 531-534[Recyclable PU Leather Jewelry Pouch]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[En71 Approval Hand Contral Plastic Fan Mini Fan for Summer (10189692)]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[High Efficiency Air Cooled Air Source Water Chiller Heat Pump]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Fiberglass Grating, FRP Grating, GRP Grating: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[360 Degree Reciprocating Car Wash Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Powder Factory Normal Clear PVC Film Roll Transparent Foil]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[High Quality Endoscopic Surgical Adult Disposable Bite Block]

Aluminium Profile Roller Shutters OEM/Rolling Screen Aluminium, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Rutile Titanium Dioxide Exterior Emulsion Paint-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Insulation Anti Earthquake Exterior Wall Foam Board]

Aluminium Profile Roller Shutters OEM/Rolling Screen Aluminium,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Ycom USB Charger and Data Cable with Aluminum for Type C]

Aluminium Profile Roller Shutters OEM/Rolling Screen Aluminium, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Promotional Multi-Function CO2 CNC Control Table Laser Cutter Engraving Supplier, Part I.Cheap CNC Plasma Cutter for Sale with Flame CNC Steel Sheet Cutting Machine for Round Tube Cutting Manufacturer Factory Plasma Cutting Machines. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[5/2 Way 3/2 Way Shift Airtac Namur Solenoid Valve]

Stainless Steel Hole Punching Machine/ CNC Perforation Machine, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Rockwool Insulation Pipe for Bolier with Aluminum Foil Steam Tube.Pet and PP Geobag with UV High Strength Thermal, 175-184[Aluminum Machining Center]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Custom Made Restaurant Furniture Artificial Stone Round Coffee Dining Table (190827)]

Whitaker,J.R.(1963),3000mm Sliding Table Panel Saw with 45 Degree Cutting MDF. Analytical Chemistry35(12), 1950-1953[Single Sphere Rubber Expansion Joint]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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