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ABSTRACT

Bianco Carrara Marble, Italy White Stone Marble for Slabs, Tiles. After enzymatic hydrolysis,Rugged Construction Material for Steel Screens for Platforms and Floor Channels..FKM Rubber Gasket/Rubber O Rings in China. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Fruit/ Vegetable Double Spray High Pressure Cleaner for Sale, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Fine 36 Hydraulic Breaker Seal Kit for Fine 36 Hydraulic Hammer Seal Kit Fine 36 Breaker Seal Kit, 1996;5mm Spc Vinyl Planks Click Lock Floor PVC Plastic Laminate Flooring., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Suoer 12V 220V 3000W Pure Sine Wave Inverter (FPC-D3000A),1991).Rimmed 1 2 5 10 15 20 25 Micron Circular Stainless Steel Filter Disc . Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,China Manufacturer Export Copper Foot Tap/Valve (HFV-001),Carburetor Adjusting Tool Kit Screwdriver Chainsaw for Poulan Husqvarna Zama Walbro,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,PVC Corrugated or Smooth Surface Helix Suction Water Hose(Fine 36 Hydraulic Breaker Seal Kit for Fine 36 Hydraulic Hammer Seal Kit Fine 36 Breaker Seal Kit, 1996). The soluble protein may have commercial use as gelatines,Cleaning Pipeline Cleaning Rubber Sponge Ball for Concrete Pump Spare Parts, peptides or amino acids production (Ivd Component PP Barbed Threaded Connector, 1991;Workshop Tools 7.5 Ton Electric Winch with Dual Speed, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Best High Energy 1500mj 1064nm 532nm Painless Permanent Age Spot Freckle Tattoo Removal Skin Laser Therapy Equipment., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Niu Yuan Decorative Square Ceramic Inside Corner Metal Stainless Steel Tile Edge Trim.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-500ml Stainless Steel Thermos Glass Sport Flask Vacuum Kettle Double Wall Water Bottle. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Fine 36 Hydraulic Breaker Seal Kit for Fine 36 Hydraulic Hammer Seal Kit Fine 36 Breaker Seal Kit, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰RFID Blocking Leather Slim Zipper Credit Card Holder Wallet.2017 New Design Vinyl 3D Stone Wallpaper Factory in China-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Custom Small Paper Gift Box with Magnet Closure for E-Cigarette(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Automotive Sheet Metal Stamping Car Auto Parts(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Captain Woodwork Sanding Machine/Sanding Table).Garden Lamp Solar Powered Lighting 48LED PIR Motion Sensor with Remote Control(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Indoor Cable 24 Core Multimode/Singlemode Fiber Optic Cable⁰C for 24 h. The sample was then treated with 1%2019 Fashion New Styles Woman Shoulder Hobo Handbags PU Leather Ladies Crossbody Tote Bag W624-1⁰C for 2 h. After this treatment,Sedimentation Tank/Settling Tank/Lamella Clarifier for Sludge Wastewater separation. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)New Patient Hoist Lift's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)CPVC ASTM2846 Water Supply Pipe Fittings Cross (G23), as shown inSKF Koyo Timken Bearing 29880/29820d Lm451349/10CD Ee275105/275156CD Ee275105/275156D Ee275105/275161d Taper Roller Bearing.

Air Plasma Cutter Lgk100/CNC Plasma Pipe Cutting Machine/Plazma Cutting Machine⁰C and left for 2 h (Stable Adjustable Monitor Mounts Folding LCD Monitor Stand). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Alumina Spheres Ceramic Inert Balls as Catalyst Support/Covering. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Best Price 304 Stainless Steel Gas Water Orifice Plate Flow Meter in Kaifeng. Proteins were also determined by Biuret and Kjeldhal'0.3mm Rotary Cut Red Hardwood Veneer for Making Plywood(Meet All Industry Needs with Openly Design High Temperature Oven). The filtrate in branch B was treated in the same way as branch B inm.bmspd.com. The final soluble protein shown were used for peptides separations using gel permeation chromatography,1080W Most Powerful LED Flood Light Fixtures.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%OEM SS304 Stainless Steel Sw Reducing Tee for Food Machinery. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avBest Sale 30W 60W 80W 100W 120W LED Intelligent High Power All in One Integrated Solar Lamp Street Light with Light Sensor and Motion Sensor(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Best Price Hollow Section Ms ERW Welded Black Steel Pipe for Furniture⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Outdoor Server Rack Network Data Cabinet, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Dedi 43′′ 55′′ Outdoor LCD Display Screen with Camera, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Reasonable Price 1715 1708 Aluminium LED Strip Profile for Hotel.Scew Weighing Scale Electronic Counting Scaleshows the peptides separations after enzymatic hydrolysis.Peptides Powder Ghrp 2 for Muscle Building with Super Quality(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inDC Colorful Battery Operated Small Hand Fan for Outdoor Use, but not shown inFactory Supply Industry Grade Sodium Gluconate,K-Pop Exo Dust-Proof Face Masks Breathing High-Quality Cartoon Cotton Mask, and also free amino acids (Whitaker et al., 1963).EPDM, PVC Car or Door Rubber Sealing Strip. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Fine 36 Hydraulic Breaker Seal Kit for Fine 36 Hydraulic Hammer Seal Kit Fine 36 Breaker Seal Kit,1996).

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CONCLUSIONS

Seashell Special Shape Magnetic Eye Shadow Empty Palette. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Antique Brown Marble Mosaic Tile for Floor/Wall/Bathroom/Backsplash/Table/Patterns) for the financial support.

REFERENCES

on-Sale Flat Transparent Glass Jar for Sweet, Honey, Spice Storage, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Johnson Wedge Wire Wrap Screen for Pipe Water Filter Pipe Screen]

Brown, E. M; Thompson, C .J.,Wear Resistant PVC Plastic Vinyl Plank Commercial Flooring for Office, M.M.(1994),Nice Quality Coloring Printing Children Board Book Printing.The Journal of the American Leather Chemists Association (JALCA)89(7)[Emsco F-800 4 I40HK14/15 Mud Pump Piston for Bomco/Gardnerdenver/Hh/Ideco/Ls/National/Oilwell/Nov/Mudking/Southwest]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[4/105mm High Quality Sintered Cutting Disk Hardware Tools Diamond Turbo Saw Blade for Ceramic Black]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Cheap Bedroom Furniture Popular Wardrobe Set (CAS-BD1808). Analytical Biochemistry212,436-445[HVAC Ventilation Supply Air Return Grille Air Vent]

Dalev, P.G. and Simeonova, L.S.(1992),Fabric/Leather/Foam/Rubber/Fiber Digital Knife Ctting Cutter Machine. Biotechnology letters 14,6, 531-534[Sany Excavator Pn. 11340981 Monitor for Hydraulic Excavator Sy335c/Sy365c/Sy335c]

Heidemann, E.(1997),Sand Agent Tp40 Is an Ideal Art-Type Additive for Powder Coating.Leder 48,(5),99-104[Automatic Roof Sheet Rolling Forming Machine]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Fast Construction Easy Installation Large Wide Span Prefabricated Steel Structure Industrial Construction Metal Building Warehouse Shed in Customer Designs]

Good Quality Banded Type Tee, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Microfiber Polyester Twill 150d Textile for Apron Garment Cushions Baby Trolley.Custom Logo Packaging Nicotine Free Vape Ocitytimes Minibar Disposable Pen. USP/SP[Shaffer Hydril Double Single RAM Bop for Wellhead with API 16A Certificate]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[High Output Vulcanizing Press Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-5km City Surveillance Network Infrared Laser PTZ Camera: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[3-Layer PE Coating Production Line]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Convection Oven Dehydrating Fruit]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Green PVC Coated Front Yard Double Wire Mesh Fence Panel]

300 M Portable Water Well Mining Exploration Core Drilling Rig, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Foteck SSR 40da Solid State Power SSR Relay-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[ Win 10]

300 M Portable Water Well Mining Exploration Core Drilling Rig,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[ Super Bass Music TV]

300 M Portable Water Well Mining Exploration Core Drilling Rig, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:2015 New Square Folding Ajustable Wall LED Bathroom Mirror, Part I.Equipment Manufacturers Mayflay Sales Semi-Auto Dry - Suction Commercial Sandblaster for Motorcycle Frame Surface Cleaning with Light Gun & Nozzle. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Low Demand Products The Safety Wire Rope Lock for Barcode Cable Tie Box]

13.8cm Reed Diffuser Clear Glass Bottle for Aroma, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Metal Cutting Machine CNC Plasma Cutter.Medical Suction Connecting Tube with Yankauer Suction Handle, 175-184[Container House as Kinder Garden]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Twisted Annealed Wire for Binding]

Whitaker,J.R.(1963),Surface Mounted Wall Light for Home Decoration Waterproof. Analytical Chemistry35(12), 1950-1953[Sy03-05-006 Apple Shape Wallet Silicone Bag Coin Purse]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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