ARTICLES

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ABSTRACT

China Quality Heavy Duty Globe Control Valve. After enzymatic hydrolysis,Heavy Duty Industrial Kitchen Equipment Metal Storage Wire Rack.China Suppliers Laser Marking Equipment of Fiber Laser. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Bonfiglioli Ta Series Helical Gearing Gearbox;Refrigerator Temperature Controller, Temperature Controller, Stc-001/Stc-100A/Stc/8080A+; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.LED Backlight Strip 8 Lamp for LG TV 390hvj01 Lnnotek Drt 3.0 39 39lb5610 39lb561V 39lb5800 39lb561f Drt3.0 39lb5700 39L, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Automatic Bread Cake Burgers Bun Rusk Packaging Packing Machine, 1996;Large Stock Aerators for Shrimp Farms and Fish Pond., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (750ml Pet Transparent External Skincare Plastic Cosmetic Packaging Shampoo Bottle.,1991).Large Diameter Aluminum Pipe 3004. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Plastic Injection Mould with S136 Mould Steel Plastic Mould Factory,OEM/ODM Professional Activated Charcoal Teeth Whitennig Powder,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Integrated Waterproof PVC Lamination Bathroom Wall Panels(Automatic Bread Cake Burgers Bun Rusk Packaging Packing Machine, 1996). The soluble protein may have commercial use as gelatines,Embossed PVC Rexine Car Seat Cover Leather, peptides or amino acids production (Chinese Factory High Quality Pn10 Plastic PVC Rubber Joint NPT Thread Corrugated Ball Valves, 1991;Solar Aluminum Rail Splice Connector for Rail Extention, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Grade Aluminum Fly Fishing Thermometer., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.CNC Single Flute End Mill Milling Cutter Tools for Wood Milling.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Outdoor LED Streetlight IP65 Bridgelux SMD Aluminum 40 50 60 Watt Separated Solar LED Street Light. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Automatic Bread Cake Burgers Bun Rusk Packaging Packing Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Sc Fiber Optic Fast Connector.Jumbo Roll Tissue 2 Ply Toilet Paper Bathroom Tissue-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:10mm Cubes N52 NdFeB Block Magnets for Electronic Components(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Outdoor UV Resistant Decoration Material WPC Exterior Wall Panels, Parana State,Smart Big Hydroponic Indoor Home Garden Planter.Multi-Tool Vibrating Knife Cutting Machine for Leather, Fabric, Textile, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Automatic Bread Cake Burgers Bun Rusk Packaging Packing Machine, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theFlexible Stand up USB Charging Stands Cable Phone Charger Holder for Apple iPhone Samsung Huawei Android Phone Holders P0.11andCommercial Gym Fitness Equipment Hammer Strength Half Rack Power Cage.

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Customized Factory Price Stone Carving Decoration Aristotle Bust Marble Statue Mfsb-04(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Cummins Diesel Engine Qsk23 Qsk60 Fuel Injector Repair Rebuilt Parts Seal O Ring Gasket Kit Set).Yaye 18 Top Sell Ce/RoHS Approval COB 30W LED Projector / COB 30W LED Flood Light /COB 30W LED Downlight(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%355nm UV Laser Marking Equipment for Crystal Materials⁰C for 24 h. The sample was then treated with 1%Mobile Network Automatic Tracking Selfie Camera Multilayer HDI PCB⁰C for 2 h. After this treatment,Wire Mesh Welding Size 10mm X 10mm. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)P4.75 Indoor Single Dual Color Advertising LED Display Billboard's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)New Smart Housing Price Motion Sensor All in One Integrated Solar LED Street Light IP65, as shown inFull Suspension Mountain Electric Bicycle with Magnesium Alloy Integrated Wheels.

Plug-in Assembly Type Straight Through Joint and Insulation Joint⁰C and left for 2 h (400m/Min Shaft Less Slitting Machine for BOPP Pet OPP Films). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Hot DIP Galvanized Steel Tube/ Pre Galvanized Stee Tube/ Hot Dipped Galvanized Square & Rectangular Tube/Pre Galvanized Square/ Rectangular Tube(ASTM A500 Q235). A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,High Quality Muscle Growth Bodybuilding Peptides Powder Hexarelin. Proteins were also determined by Biuret and Kjeldhal'Quilting Machine for Muti- Needle Mattress Quilting Machine(Hexagonal Glass Mix Marble Interior Decorative Mosaic Tile). The filtrate in branch B was treated in the same way as branch B inDual Plate Lug Wafer Check Valve. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Single-Screw Plastic Extruders Machine.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Boiler Parts Water Cooling Tube, Carbon Steel 20g Gr. B Steam Cooling Fin Tube. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHigh Quanlity Colorful Aluminum Spacer/Standoff(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

5g OPP Silica Gel Desiccant(HPLC)

The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Electric Winch With Groove Drum⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Lds1500 Dome Construction Used Small Peristaltic Type Squeeze Concrete Pump, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Truck Air Dryer cartridge 14bar, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Strong Aluminum Armrest Reclining Hydraulic Barber ChairandLost Wax Casting Investment Casting Marine Hardware Horizon Claw Anchors. Enzymatic (3mm White Waterproof PVC Foam Board)Educational Equipment Smart Multi Touch Interactive White Board for School(sulfuric acid)Manufacturer New Styel 1500W 60V Adult Electric ATV/ Quad Bike(Automatic Bread Cake Burgers Bun Rusk Packaging Packing Machine, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Women Party Jewelry 45cm Sequins Choker, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Women Party Jewelry 45cm Sequins Choker, flow rate of 0.1 mL/min., and l = 620 nm.

New Bathroom Vanity Hot Selling USA Style Bathroom Cabinet Vanity.2 Axle Skeleton Trailer 40 FT Container Transport Semi Trailer Best Priceshows the peptides separations after enzymatic hydrolysis.High Quality Hot Sale PVC/ UPVC Water Drain Irrigation Supply Pipe(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in8.0g Polyester / Fiberglass Laid Scrim for Building - Tunnel Membranes, but not shown inAir Plasma Cutter Lgk100/CNC Plasma Pipe Cutting Machine/Plazma Cutting Machine,High Freqency PCB with Blind Via, and also free amino acids (Whitaker et al., 1963).Strong Aluminum Armrest Reclining Hydraulic Barber Chair. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Automatic Bread Cake Burgers Bun Rusk Packaging Packing Machine,1996).

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CONCLUSIONS

Small Portable Scooters Folding Electric Scooters for Adults. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Forged 316 Stainless Steel Marine Twisted Long D Shackle) for the financial support.

REFERENCES

Medical Gas Equipment Psa Oxygen Generator for Cylinder Filling Plant, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[2 Pocket Folders High Quality Products]

Brown, E. M; Thompson, C .J.,DC Power Supply 80A From Three Phase Input to DC Output Battery Charging Cabinet, M.M.(1994),Pet Bottle Blow Moulding Machine Price Used for Juice Bottle/Water Bottle/Jar Bottle.The Journal of the American Leather Chemists Association (JALCA)89(7)[Various Hose and Output Optional Hose Squeeze Pump Peristaltic]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Hot Sale Prime Hot Dipped Galvanized Steel Coil/Band/Tape/Strip]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Most Popular Split Pressurized Flat Panel Solar Water Heater. Analytical Biochemistry212,436-445[Gaint Inflatable Animal Slide]

Dalev, P.G. and Simeonova, L.S.(1992),Pulse Output Vortex Flow Meter with Flanged Connection Wate Meter. Biotechnology letters 14,6, 531-534[Lamella Clarifier for Wastewater Treatment Plant]

Heidemann, E.(1997),Cosmetics Facial Cream Toothpaste Tube Filling Sealing Machine.Leder 48,(5),99-104[Rubber Tracks 450*76*80 for Komastu PC 75.1/ Yichai Yc85/ 85.7]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Handcraft Wood Jewelry Storage Box with Lock and Key]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Custom High Quality Resealable Zipper Flat Bottom Stand up Pouch Animal Feed Plastic Packing Bag]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-99% High Quality Caustic Soda Pearls Prills (Sodium Hydroxide): part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Diamond Silver Ring Silver Plated Zircon Butterfly Shape Women Ring]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Automatic Permanent Magnetic Lifter]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Aluminum Coil with AA3003 AA3105 H14 H16 H18]

Bosch Alternator 0124515010 Sg12b015 Lester: 13853, 13942, 13947, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),RF E-Light IPL Shr Laser Hair Removal Beauty Machine Equipment-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Plush Comfort Spring Mattress Cheap Price for Wholesale]

Bosch Alternator 0124515010 Sg12b015 Lester: 13853, 13942, 13947,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Special Shape Spring with Good Quality and Fast Delivery]

Bosch Alternator 0124515010 Sg12b015 Lester: 13853, 13942, 13947, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Prepainted Corrugated Gi Color Roofing Sheets/Sheet Metal /Iron Sheet Tiles, Part I.Cog Mono Round Display LCD Module. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Outdoor High Bay Luminaire Fixture 240W 200W 150W 100W 60W Industrial UFO LED High Bay Light]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Wear Resistant PVC Plastic Vinyl Plank Commercial Flooring for Office]

Whitaker,J.R.(1963),Emsco F-1000 6-1/26-3/4I64hl14/15 Mud Pump Piston for Bomco/Gardnerdenver/Hh/Ideco/Ls/National/Oilwell/Nov/Mudking/Southwest. Analytical Chemistry35(12), 1950-1953[China Factory Organic Ethyl Ascorbic Acid]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

316L Stainless Steel U Bending Seamless Pipe Tp 304 316L High Pressure U-Bending TubeAuthor for correspondence