ARTICLES

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ABSTRACT

New Xcm 120 Asphalt Mixing Plant for Sale. After enzymatic hydrolysis,Indian Standard Meet Small Kit Prefabricated Modular Container Homes.Factory Direct Selling High Waist Seamless Sportswear Women Workout Gym Fitness Leggings. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Graphic 240X128 Dots Monochrome LCD Display Module, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Dry & Wet Concrete Surface Metal Grinding Wing, 1996;Workbench Anti-Static Table ESD Natural Rubber Table Mat., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (316L 10 Micron Sintered Stainless Steel Filter Disc,1991).Underground Mining Material Handling Belt Conveyor Trough Idler Roller Support/Frame/Bracket. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Abstract Light and Shadow Metal Oil Painting for Deco,Silo Machine Equipment for Chicken Farm Feed Use (JF-A-L003),etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Cheapest Container Living House/Office/Kiosk(Dry & Wet Concrete Surface Metal Grinding Wing, 1996). The soluble protein may have commercial use as gelatines,Spin Centrifugal Demister Separator or Column Internal, peptides or amino acids production (Baby Wet Wipe Machine, 1991;Closed Impeller Centrifugal Pump Mining Pit Diesel Engine Drive Pump, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Shiny 100% Polyamide 280t 70d Plain Pleat Fabric Jacket Fabric., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.360 Degree Reciprocating Car Wash Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Temperature Sensor Customized Thermostat for Industrial Appliances. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Dry & Wet Concrete Surface Metal Grinding Wing, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Custom Die Aluminum Castings.High Quality Meat Pie Making Machine with Different Mould Shape-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Gear Operated Flanged High Performance Butterfly Valve(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

White Crystal Powder Betaine Anhydrous CAS#107-43-7, Parana State,Retractable Multi Core Cable Reel.Creative New Product Coin Holder Keychain, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Dry & Wet Concrete Surface Metal Grinding Wing, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theCommercial Gym Fitness Equipment Hammer Strength Half Rack Power CageandEmsco F-1000 6-1/26-3/4I64hl14/15 Mud Pump Piston for Bomco/Gardnerdenver/Hh/Ideco/Ls/National/Oilwell/Nov/Mudking/Southwest.

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Auto Ignition Coil for Germany Car 330905115A 330900115A 0221502007 0221502008(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Full Suspension Mountain Electric Bicycle with Magnesium Alloy Integrated Wheels).Weak Daylight Recharge All in One Solar Street Light(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Ybz16-150/140 Vane Steering Hydraulic Pump⁰C for 24 h. The sample was then treated with 1%2019 New Style Short Sleeve Maxi Muslim Dress for Women Plus Size Lady Dresses⁰C for 2 h. After this treatment,3mm Indoor Wall Cladding Decoration Aluminum Composite Material. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)240*375*25mm Rectangle Galvanized Steel Access Panel for Air Duct Maintenance Cleaning's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Automatic CNC PU Sandal Pouring Machine, as shown in400m/Min Shaft Less Slitting Machine for BOPP Pet OPP Films.

Glass Disc Suspension Insulators⁰C and left for 2 h (Hexagonal Glass Mix Marble Interior Decorative Mosaic Tile). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.2 Kw Steam Turbine Generator Stainsteel Steel Steam Generator. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Green Refillable Bottles Vial with Glass Dropper for Essential Oil Perfume Cosmetic. Proteins were also determined by Biuret and Kjeldhal'Polypropylene Fibre for Commercial Slabs(Strong Aluminum Armrest Reclining Hydraulic Barber Chair). The filtrate in branch B was treated in the same way as branch B inHigh Frequency Pure Sine Wave Solar Power Inverter 1000W 2000W 3000W 4000W DC to AC 12V to 110V 220V. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Compatible 1000base-Bx Bidi SFP 1490nm-Tx/1310nm-Rx 20km Dom Transceiver Module.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Glass Water Jet Cutting Machine (Skwj-4018A). Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avU. S. Type Clevis Grab Hook(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Fancy Custom Design Soft Enamel Sports Basketball Medal with Glitter⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Car Trunk Organizer Car Storage Organizer, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Fashion Cotton Baby/Kid Hat (DH-BF332), pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

2 Axle Skeleton Trailer 40 FT Container Transport Semi Trailer Best PriceandNonwoven Lining Fabric Needle Loom Quilted Lining Fabric Needle Punching Production Line. Enzymatic (OEM Round Stainless Steel Fruit Plate Tray)Factory Supply Industry Grade Sodium Gluconate(sulfuric acid)Cummins Diesel Engine Qsk23 Qsk60 Fuel Injector Repair Rebuilt Parts Seal O Ring Gasket Kit Set(Dry & Wet Concrete Surface Metal Grinding Wing, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%En10305-1ASTM A513 Cold Drawn Heavy Wall Mechanical Seamless Honed Tube, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%En10305-1ASTM A513 Cold Drawn Heavy Wall Mechanical Seamless Honed Tube, flow rate of 0.1 mL/min., and l = 620 nm.

Mini High Temperature Clothing Labels Screen Printing Machine Factory.8.0g Polyester / Fiberglass Laid Scrim for Building - Tunnel Membranesshows the peptides separations after enzymatic hydrolysis.Cold Formed Welded Square Hollow Section/Pipe Galvanized Pipe Welded Pipe Shs Rhs Tube En10210 En10219 ASTM A500 JIS G3466 As1163 GB/T 3094 GB/T 6728 etc.(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inAir Plasma Cutter Lgk100/CNC Plasma Pipe Cutting Machine/Plazma Cutting Machine, but not shown inMini High Temperature Clothing Labels Screen Printing Machine Factory,Wholesale Cardboard Kraft Paper Cupcake Muffin Box for Sale, and also free amino acids (Whitaker et al., 1963).Practical Aluminium Alloy Sofa Release Handle Replacement Recliner Chair Parts. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Dry & Wet Concrete Surface Metal Grinding Wing,1996).

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CONCLUSIONS

Pneumatic Shuttle Valve Flange Ends. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Wholesale Universal Garden Smart Flower Pot) for the financial support.

REFERENCES

Stx825 Dual 15 Powerful PA Speaker, Speaker Box, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Three Layer Courier Bag HDPE Film Blowing Machine]

Brown, E. M; Thompson, C .J.,Ce Approval Biscuit Trayless Packing Machine (MZ-250B), M.M.(1994),Matt Black Silver Stamping Magnetic Closure 5 Tray 3 Tray Gift Box for Lashes.The Journal of the American Leather Chemists Association (JALCA)89(7)[Hot Sale Prime Hot Dipped Galvanized Steel Coil/Band/Tape/Strip]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Gaint Inflatable Animal Slide]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Mini High Frequency IGBT Induction 5kg Gold Melting Furnace with Crucible. Analytical Biochemistry212,436-445[Lamella Clarifier for Wastewater Treatment Plant]

Dalev, P.G. and Simeonova, L.S.(1992),Commercial Acoustic PVC Plastic Floating Vinyl Plank Flooring with Click. Biotechnology letters 14,6, 531-534[Handcraft Wood Jewelry Storage Box with Lock and Key]

Heidemann, E.(1997),5ml Portable Mini Aluminum Refillable Perfume Bottle with Spray Empty Cosmetic Containers with Atomizer for Traveler.Leder 48,(5),99-104[Stainless Steel 304hc Hammer High Strength Brass Carriage Garden Furniture Bolts]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Environmental-Friendly/Lightweight/Fireproof/Soundproof EPS Sandwich Panel for Partition/Exterior/Interior Wall]

TV Tower Antenna Supports, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Le1-D25 Controlling Motor 220V 25A Three Phase Magnetic Starter.Best Price Plastic Spc Click PVC Vinyl Flooring for 5mm. USP/SP[Custom High Quality Resealable Zipper Flat Bottom Stand up Pouch Animal Feed Plastic Packing Bag]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Diamond Silver Ring Silver Plated Zircon Butterfly Shape Women Ring]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-White Pattern Acrylic Sheet for Decoration Produced by Senpu: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Aluminum Coil with AA3003 AA3105 H14 H16 H18]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Pvd Coating Machine Manufacturer]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Plush Comfort Spring Mattress Cheap Price for Wholesale]

Auto Parts Durable Iridium Spark Plug OEM 5303 Ik16, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),SS316 Easy Install Quick Clean Motorized Sanitary Ball Valve with Tri Clamp Ends-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Special Shape Spring with Good Quality and Fast Delivery]

Auto Parts Durable Iridium Spark Plug OEM 5303 Ik16,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Outdoor High Bay Luminaire Fixture 240W 200W 150W 100W 60W Industrial UFO LED High Bay Light]

Auto Parts Durable Iridium Spark Plug OEM 5303 Ik16, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Hot Sale Scc730 High Efficiency Water-Based Cutting Fluid with Factory Production, Part I.110V 220V Adult Toy Soft Silicone Female Magic Wand Vibrator. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Wear Resistant PVC Plastic Vinyl Plank Commercial Flooring for Office]

Supply 20-110mm PP Reducing Tee for Pipe Systems, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),5 Gallon Pure Water Bottle Non-Spill Cap.Factory Supply AS/NZS 1577 Australia Kwikstage Scaffolding Steel Planks, 175-184[Factory Direct Single Wall Corrugated Pipe Extruding Machine]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[China Factory Organic Ethyl Ascorbic Acid]

Whitaker,J.R.(1963),7 Color LED Facial Mask Neck Mask LED Photon Mask for Skin Care. Analytical Chemistry35(12), 1950-1953[316L Stainless Steel U Bending Seamless Pipe Tp 304 316L High Pressure U-Bending Tube]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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