ARTICLES

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ABSTRACT

Ge-Ding High Voltage Reed Relay Grl 24V Z1-Grl-2412 1X10^10ohm Gd. After enzymatic hydrolysis,Auto Defrost Supermarket Island Freezer Merchandiser with Integral Compressor.8 Inch Computer Program Control 78cm Paper Cutter (QZK780D-8). Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Custom Cheap Marathon Sport Towel Microfiber Slogan Towel for Promotion;Maleic Anhydride CAS 108-31-6; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Plastic HDPE Pipe Extrusion Making Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Jaguar Jb-390 Automatic PCB Loader and Unloader, 1996;Scew Weighing Scale Electronic Counting Scale., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (China Factory Organic Ethyl Ascorbic Acid,1991).Aluno Aluminum Veneer Price Aluminium Curtain Wall Details. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Factory Price Sodium Gluconate 98% for Concrete Admixture,Pneumatic Cut off Ball Valve with Flange Thread Weld End,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Coin Operated Whack The Mole Lottery Machine Hit Hammer Game Machine(Jaguar Jb-390 Automatic PCB Loader and Unloader, 1996). The soluble protein may have commercial use as gelatines,Lead -Free Salt Pepper Glass Jar Kitchenware Jar White Pepper Glass Jar, peptides or amino acids production (Good Price Sc Single Mode Simplex Duplex Fiber Optic Adaptor, 1991;Decorative Aluminum Wall Cladding Panel with Durable Painting Finish, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Steel Grating for Industrial Platform., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.50Hz/60Hz Low Frequency Home Power Inverter 5000W.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-ANSI Standard Disc Suspension Insulator (52-1) for Transmission Line. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Jaguar Jb-390 Automatic PCB Loader and Unloader, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Hot Sale Wholesale Custom Logo Luxury Paper Gift Box with Magnet Closure.Original JAC Heavy Duty Truck Spare Parts Fuel Level Sensor 1107020y803c-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Rigid PVC/ Rigid Floor /Rigid Flooring /Spc Flooring(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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From Chongqing by Great Manufacturer Excellent Extrusion and Stamping Die(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Cheap Loader Xt860 Backhoe Loader with Digger).Mini Double Hole ABS Plastic CNC Machined Parts Importer(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%KZ-19/16/13 Pneumatic Combination Steel Binding Machine⁰C for 24 h. The sample was then treated with 1%Medical Packing Self Sealing Sterilization Pouch⁰C for 2 h. After this treatment,Water Filter Cartridge PP Sediment Filter Cartridge. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Wholesale Military Black Color Clothing /Army Bdu Uniforms Surplus Stock for Sale's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Disposable Skin Stapler (CSPF-35W), as shown inMetal Parts Stamping.

Custom Made Restaurant Furniture Artificial Stone Round Coffee Dining Table (190827)⁰C and left for 2 h (55 Inch Floor Stand in Store Outdoor LCD Digital Signage Display Monitor Touch Screen Kiosk with PC). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.New Customized Mug Heat Press Machine Price. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,All-Automatic Powder Wrapping Machine for Tomato Sauce, Lotion Noodle Jam. Proteins were also determined by Biuret and Kjeldhal'Wholesale Plastic Pet Bowl with a Cap Pet Basin Bowl Factory(GB11638 10L 40L C2h2 Low Pressure Acetylene Cylinder with Valve). The filtrate in branch B was treated in the same way as branch B inProducts. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Shengya Steel Concrete Electric Pole Mould.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Outdoor Enjoy Music V4.2 Mini Wireless Stereo Bluetooth Speaker. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avQuick Field Assembly Fiber Optic FC Mechanical Fast Connector for FTTH Cable(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.China Manufacturer Garbage Incinerator⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/China water meter factory water flow meter vane wheel, pH 3.25., mobile phase B: buffer solution of 0.01 mol/4 Heads High Speed Professional Paper Cardboard Laser Cutter, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Mining Machinery Parts Diamond Saw Blade Segments for MarbleandIndustrial High Pressure Food Quality Rubber Hydualic Hose for Water Foodshow the gel chromatographic separation of soluble protein with Sephadex G-Screw Press Dewatering Machine for Handling The Reject From Paper Mill.C Type Small Simple Power Press Tools Machine with Foot Pedal Switch of Manufactuer Suppliers and Importer for Sale, and another peak at 45 mL not shown inHigh Quality Automatic Snack Food Lab Twin Screw Extruder Price, for peptides lower than 3000 Da.4lz-4.5 Small Tank Kubota Similar Rice Harvester(Condell et al.,1993) .

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CONCLUSIONS

Stainless Steel Whisky Chilling Stones. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Shs250X500 Galvanized Square Rectangular Steel Tube Price) for the financial support.

REFERENCES

Nmsafety TPR Back Cut Resistant Oil and Gas Impact Work Glove, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Dakiou Low Speed Paper Cup Making Machine]

Brown, E. M; Thompson, C .J.,CNC Automatic Pneumatic Tapping Machine/ Drilling Machine for Fasteners, M.M.(1994),Automatic 150m/Min Multi Color Uesd Motor Roto Gravure Rotogravure Printing.The Journal of the American Leather Chemists Association (JALCA)89(7)[IPL Hair Removal Skin Rejuvenation Beauty Machine (IPL+E-light Function HS-650)]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Shiny 100% Polyamide 280t 70d Plain Pleat Fabric Jacket Fabric]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Good Quality Custom Car Power Window Cable for Ford Car. Analytical Biochemistry212,436-445[Large Stock Aerators for Shrimp Farms and Fish Pond]

Dalev, P.G. and Simeonova, L.S.(1992),Solar Panel PV System Mc4 Circular Connector. Biotechnology letters 14,6, 531-534[High Quality Meat Pie Making Machine with Different Mould Shape]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[2019 Wholesale Electroplate Purple Glass Votive Candle Jar for Holidays]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Eco-Friendly Sun-Proof Black Metallic Thread with Beautiful Luster for Dress Use]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Boiler Fin Tube, Hot Steam Ot Water Cooling ASME SA179 SA192 Fin Tube Wall: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[LED Fairy Star Light for All Kinds of Decoration Skylike]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Aluminum Chlorohydrate Liquid (ACH) for Water Treatment]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Automotive Sheet Metal Stamping Car Auto Parts]

Plastic Recycling Centrifugal Dewatering Machine, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Biostimulant Manufacturer Natural Seaweed Extract Liquid Fertilizer-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Excavator Transportation Gooseneck Low Bed Semi Trailer 3 Axles]

Plastic Recycling Centrifugal Dewatering Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[3-in-1 Automatic Bottled Pure Mineral Water Filling Equipment for Gallon Bottle]

Plastic Recycling Centrifugal Dewatering Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:EAS Anti-Theft Optical Security Tag for Glasses Bottle, Part I.Single Phase Digital Electric Power Meter. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[China Suppliers Laser Marking Equipment of Fiber Laser]

Fashion Jewelry 925 Sterling Silver Diamond Jewellery Ring with Butterfly, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),2W 100lm LED Solar Lawn Light for Garden or Yard Lighting.Single Phase 220V AC 3 1.8kw Qdx Submersible Clean Water Pump, 175-184[Copper Fitting]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Manual Double Bridge Glass Cutting Table Machine (JFQG-2620)]

Whitaker,J.R.(1963),28mm China High Quality Supplier Screw Cannon Cap for Bottle. Analytical Chemistry35(12), 1950-1953[Hot Selling Fgg. 0b. 309 Metal Circular Push Pull Connector]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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