ARTICLES

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ABSTRACT

Fashion Design Sleeveless Fitness Wear. After enzymatic hydrolysis,DC Colorful Battery Operated Small Hand Fan for Outdoor Use.Hsc Heavy Duty 5 Ton Chain Pulley Block. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.China Cheap Newest 70*77cm XPE Foam 3D Wallpaper, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (48V 200ah UPS Battery Backup for Telecom, 1996;450ml Can Beer Tin Take Away 2 Pack Shipped on Beer Tray., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Rustic Burnt Wood Coffee Mug Hanging Rack with 8 Hooks,1991).5 in 1 Hydra Facial Peeling Micro Dermabrasion Skin Care Skin Whitening Multifunction Beauty Equipment. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Plastic Concrete Beige PVC Wall Panels Decorative Waterproof for Bathrooms,8L Mini Fridge Freezer for Home and Car Fridge,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Rotary Axis CNC Engzving Router with Ncstudio Controller Qd-6090r(48V 200ah UPS Battery Backup for Telecom, 1996). The soluble protein may have commercial use as gelatines,23m 4 R Arms 2017 New Design Concrete Placing Boom, peptides or amino acids production (Wholesale Coffee Tool Foam Maker Stainless Steel Milk Frother with Best Filter Durable Handle, 1991;China Factory Offer New Core I7 15.6 Laptop, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Ce Approved Top Quality Hot-DIP Galvanized Pig Sow Farrowing Crates., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Mining Machinery Parts Diamond Saw Blade Segments for Marble.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Releasable/Resuable Stainless Steel Metalic Tie Strap for Binding. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (48V 200ah UPS Battery Backup for Telecom, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰China Plastic Grid for Paver Grass for Gardening.Explosion Proof Rechargeable LED Searchlight-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Tongkat Ali Root Extract 200 1, Tongkat Ali 200: 1(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Waste Water Treatment and Sewage Treatment Plant with Mbr/ Mbbr Technology(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Various Hose and Output Optional Hose Squeeze Pump Peristaltic).Table Planetary Saw Blade Alloy Steel Pipe Tube Cold Cuter(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Weaving Poly Wicker Cookeware Gas Fireplace Rattan Fire Pit Table Set Furniture⁰C for 24 h. The sample was then treated with 1%China Supplier Solar LED Street Light Price Ce RoHS (SX-TYN-LD-15)⁰C for 2 h. After this treatment,Fresnel Lens for Pyroelectric PIR Infrared Radial Sensor. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Rubber Tire Plastic Recycling Shredder with Factory Price's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)China Manufacturer Custom Stainless Steel CNC High Precision Linear Shaft, as shown inHot Sale Prime Hot Dipped Galvanized Steel Coil/Band/Tape/Strip.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%High Quality Rigid-Flex PCB Board Flexible Printed Circuit Manufacturer PCB. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avDC Stand Fan Motor PCBA Card 24V(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Stainless Steel 304 Wire Rope Fork Terminal⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Custom Logo Aluminum Foil 500g Coffee Food Bean Food Pouch Bag, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Color Plastic Acrylic Sheet Plexiglass PMMA Acrylic Sheet, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Paper or PVC Mobile or Cellphone Scratch Recharge Prepaid Card, V_e, and K_avcalculated as discussed previously.Blue Silica Gel with Indicating,Hydraulic Three Roll Mill with Hydraulic Squeeze Pressing system. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (China Factory Organic Ethyl Ascorbic Acid) (Reis et al.,1989;Wholesale Coffee Tool Foam Maker Stainless Steel Milk Frother with Best Filter Durable Handle.,1991;450ml Can Beer Tin Take Away 2 Pack Shipped on Beer Tray.,1991).Top Security Disc Key Anti-Rust Cylinder Pull up Handle Touch Screen Coffee Vending Machine Lock(1992), were lower, except for cystine and tyrosine,Rechargeable Lightweight Running LED Headlamp Torch (SYSG-190219).

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CONCLUSIONS

Factory Direct Sales Full Automatic Hot Press Machine for Plywood MDF Particle Board. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Forging Metal Building Parts) for the financial support.

REFERENCES

High Precision Green Sand Casting Ball Valve Parts, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[zichen-rubber.com]

Brown, E. M; Thompson, C .J.,Bypass Shower Door Screen with Stainless Steel 304 Frame Hardware, M.M.(1994),Class 300 1/2 Stainless Steel Forging Type Check Valve.The Journal of the American Leather Chemists Association (JALCA)89(7)[Table Planetary Saw Blade Alloy Steel Pipe Tube Cold Cuter]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[10mm Cubes N52 NdFeB Block Magnets for Electronic Components]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-CAD Cam Dental Machine 3D Dental Model Printer. Analytical Biochemistry212,436-445[High Quality Segmented Cold-Pressed Concrete Diamond Circular Cutting Disc]

Dalev, P.G. and Simeonova, L.S.(1992),900kg Pneumatic Motorcycle Lift. Biotechnology letters 14,6, 531-534[Stainless Steel CF8/CF8m Street Elbow Pipe Fittings]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Ultra Slim Color Pouch Bag Cover Sleeve Case for iPad PRO 9 Inch Tablet]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Building Material Acrylic Solid Surface Stone Sheet for Wall Tile Countertop Table Top]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-New Vmc Machine Auto Parts Processing CNC Vertical Machining Centre: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[High Quality 33kv 6.3kv 1000 kVA 50% Copper and 50% Aluminum Material Distribution Transformer Made in China]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[1u Case]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Indoor Inkjet Media Material]

Hot Selling Porcelain White Coffee Mug with Electroplate Handle Custome Printed Character, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Etched Stainless Steel Titanium Sheet-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Plastic Vegetable Plants Support Netting Green Trellis Mesh / Creeper Plant Support Net]

Hot Selling Porcelain White Coffee Mug with Electroplate Handle Custome Printed Character,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[1080W Most Powerful LED Flood Light Fixtures]

Hot Selling Porcelain White Coffee Mug with Electroplate Handle Custome Printed Character, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Heat Protection Thermal Insulation PTFE Coated Aluminized Fiber Glass Fabric, Part I.Ceramic Tile Cutting Tool Diamond Saw Blade with Hot Pressed. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Group 152cm Picnic Folding in Half Table]

Cold Rolled Galvanized Carbon Hot Welding Stainless Steel Pipe Round Seamless Stainless Steel Tube (403/408/409/410/416/420/430/431/440/440A/440B/440C/439/443), J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),33kv Electrical Automatic Voltage Regulator.4 Gang 1 Way Electrical Fitting Ce Certificate, 175-184[Screw Round Threading Dies]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Extra Heavy Wall Thickness Seamless Tubes]

Whitaker,J.R.(1963),High Temperature Resistance Diamond Wire Saw for Granite Quarry Diamond Wire Rock Saw. Analytical Chemistry35(12), 1950-1953[Office Supplies Flexible Plastic Writing Clip Board PP Foam Clipboard]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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