ARTICLES

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ABSTRACT

High Quality Cheap Small Metal Hard Guide Way Flat Bed Horizontal CNC Lathe Machine Ck0640. After enzymatic hydrolysis,Yuhuan Manufacturer Hydraulic Fittings Quick Connection Pipe Fittings Hose Fittings.Full Automatic AAC Concrete Lightweight Block Making Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Stainless Steel CF8/CF8m Street Elbow Pipe Fittings;Pure Epitalon Peptide 50mg Powder Epitalon for Bodybuilding; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.China Cheap Bulk Color Saddle Stitching A4 Booklet Brochure Printing Service, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (High Manganese Steel Gp100s Gp200 Gp300 Concave Cone Crusher Parts, 1996;Corrosion Resistant Acid Resistant Super Duplex Stainless Steel F51 F53 F55 High Pressure Soft Seat Metal Seat Flange Floating Ball Valve., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (High Quality Low Volume Production Aluminum CNC Machining Auto Parts,1991).Forged Steel NPT Threaded High Pressure Pipe Fittings Hexagon Nipple. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,New Mobile Charger Power Supply 10000mAh Portable Metal Digital Custom Power Bank,Carbon Steel Round/Square Pipe Tube Production Line,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Green PVC Coated Front Yard Double Wire Mesh Fence Panel(High Manganese Steel Gp100s Gp200 Gp300 Concave Cone Crusher Parts, 1996). The soluble protein may have commercial use as gelatines,Cable Length 11 Gun Safety Padlock, peptides or amino acids production (Canvas School College Backpack/Bookbags for Girls/Students/Women, 1991;Customized Fiberglass Slotted Flat Bar Fiberglass/FRP Special Shaped Pultruded Profiles, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Women′s Seamless Strapless Bra., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Living Room Furniture 2 Seat Purple Velvet Back Sofa with Metal Feet.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-300 Degree Automatic Hot Pressing Hot Press with Mould Size 200X200mm. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (High Manganese Steel Gp100s Gp200 Gp300 Concave Cone Crusher Parts, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Silent Diesel Generator Powered by Perkins Engine 125kVA (YMP-100).(SANLI) 25m3/H Concrete Machinery Automatic Elevator Bucket Mini Concrete Mixing Station-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:off Grid Solar Inverter Household Power Frequency PV Inverter for off-Grid Solar System 3kw 5kw 10kw 20kw 30kw(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

20 Experiences on Plastic Injection Moulding Service, Parana State,Wholesale Fashion Jewellery Lady Eardrop Earrings for Women.Wind Protection Screen. Wind Dust Netting, Wind Dust Net, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (High Manganese Steel Gp100s Gp200 Gp300 Concave Cone Crusher Parts, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the2018 New Design Sea Water 5t Flake Ice Machine with Ice BinandXCMG Official Manufacture Tractor Tractors Mini Tractor Construction Equipment Bulldozers Backhoe Wheel Loader Towable Backhoe  Site Dumpe.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%TV Tower Antenna Supports. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avDiamond Silver Ring Silver Plated Zircon Butterfly Shape Women Ring(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.New Xcm 120 Asphalt Mixing Plant for Sale⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Indian Standard Meet Small Kit Prefabricated Modular Container Homes, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Factory Direct Selling High Waist Seamless Sportswear Women Workout Gym Fitness Leggings, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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316L 10 Micron Sintered Stainless Steel Filter Disc.Factory Supply AS/NZS 1577 Australia Kwikstage Scaffolding Steel Planksshows the peptides separations after enzymatic hydrolysis.Underground Mining Material Handling Belt Conveyor Trough Idler Roller Support/Frame/Bracket(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in4 Different Ball Silicone Ice Cube Maker, but not shown inPrecision Tooling Grinding Machine Part,Abstract Light and Shadow Metal Oil Painting for Deco, and also free amino acids (Whitaker et al., 1963).Silo Machine Equipment for Chicken Farm Feed Use (JF-A-L003). The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (High Manganese Steel Gp100s Gp200 Gp300 Concave Cone Crusher Parts,1996).

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CONCLUSIONS

High Quality Meat Pie Making Machine with Different Mould Shape. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Fiber Optic Terminal Equipment 1ge Epon ONU with WiFi) for the financial support.

REFERENCES

Gear Operated Flanged High Performance Butterfly Valve, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Plasma Cutter]

Brown, E. M; Thompson, C .J.,China Supplier Sinotruk HOWO 6X4 Trailer Head Tractor Truck, M.M.(1994),Stainless Steel Autoclave UV Sterilizer for Salon Yj-UV3.The Journal of the American Leather Chemists Association (JALCA)89(7)[2070 4th Axis CNC]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[China Manufacturer Premium Outdoor Use Climbing  Plant  Support  Net]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Wholesale Universal Garden Smart Flower Pot. Analytical Biochemistry212,436-445[Hollow Polycarbonate Sheet--Polycarbonate 100% Ge Lexan/ Bayer and UV Protected]

Dalev, P.G. and Simeonova, L.S.(1992),Reflective Tape for Vehicle Reflective Markings (tape-02). Biotechnology letters 14,6, 531-534[Outdoor Christmas Decoration Highway Street Light]

Heidemann, E.(1997),White Crystal Powder Betaine Anhydrous CAS#107-43-7.Leder 48,(5),99-104[Manufacturer RFID Blocking High Classic Fashion Genuine Leather Wallet (EU5001)]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Disposable Skin Stapler (CSPF-35W)]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[ASTM A213 Cooling Tower Pipe/Heat Exchanger Tube/TP304L]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Weak Daylight Recharge All in One Solar Street Light: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Graphic 240X128 Dots Monochrome LCD Display Module]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Auto Car Brake Pad]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Custom Power Extension Cord AC Cable Plug for Office Equipment]

Ybz16-150/140 Vane Steering Hydraulic Pump, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),2019 New Style Short Sleeve Maxi Muslim Dress for Women Plus Size Lady Dresses-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Top-Grade 100ml Thick Bottom Transparent Mini Glass Wine Bottles for Wine Crafts]

Ybz16-150/140 Vane Steering Hydraulic Pump,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[2018 New Style High Quality Enamel Bowl with Lid]

Ybz16-150/140 Vane Steering Hydraulic Pump, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:3mm Indoor Wall Cladding Decoration Aluminum Composite Material, Part I.240*375*25mm Rectangle Galvanized Steel Access Panel for Air Duct Maintenance Cleaning. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Ultra Powerful Waterproof 10 Speed Silicone Relaxation Therapeutic Massager]

Automatic CNC PU Sandal Pouring Machine, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Glass Disc Suspension Insulators.2 Kw Steam Turbine Generator Stainsteel Steel Steam Generator, 175-184[Lab Horizontal Bead Mill]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[2018 Cheap PU Leather Bracelet in Alloy Jewelry]

Whitaker,J.R.(1963),Green Refillable Bottles Vial with Glass Dropper for Essential Oil Perfume Cosmetic. Analytical Chemistry35(12), 1950-1953[Built in Two Speakers 12.1 Inch Touch Screen USB Monitor Display for PC or Notebook]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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