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ABSTRACT

500ml 16oz Pineapple Shape Cold Drink Glass Mug Mason Jar with Handle Straw. After enzymatic hydrolysis,Popular Golden Sequin Fabric Eembroidery Lace Trimming for Wedding Dress.1200X1000X810 Strong Collapsible Plastic Containers for Storage. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Fast Install Polyurethane Decorative Wall Panel for Homes;Customized Factory Supply Stainless Steel T Shape Bolt; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Hot Sale Straight Dried Instant Chinese Wheat Flavor High Gluten Noodle, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Ultra Slim Color Pouch Bag Cover Sleeve Case for iPad PRO 9 Inch Tablet, 1996;Cracking Acidification Clay Stabilizer Pale Yellow Liquid., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (High Tensisle Strength Tissue Paper,1991).High Zinc Coated Galvanized Wire. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Women Summer Swimsuit Chest Zip Bathing Suit,Abstract 2019 New Design (DSC_4597) Handmade Oil Painting Wall Decorative Art,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Female Header Conn Ptich 1.0mm Connector Btb(Ultra Slim Color Pouch Bag Cover Sleeve Case for iPad PRO 9 Inch Tablet, 1996). The soluble protein may have commercial use as gelatines,Tie Down Nylon Back to Back Hook and Loop Straps, peptides or amino acids production (Dump Truck HOWO Tipper Truck 25ton Capacity Sinotruk Truck, 1991;42/57/60/86/110mm 24V 36V 48V 60V BLDC Brushless DC Electric Motor, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Ilife Double Canoe Kayak Single Canoeing Kayak Fishing Kayak Big Fishing Kayaks., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Industrial Automatic Best Price Professional Corn Curls Kurkure Cheetos Nik Naks Corn Puff Snacks Food Extruder Machine Electric Gas Popcorn Making Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-High Quality Warm Paste Packaging Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Ultra Slim Color Pouch Bag Cover Sleeve Case for iPad PRO 9 Inch Tablet, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Stainless Steel Resin Trap Strainer.Small Bar Fridge with Glass Door for Beverage and Beer-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Fcatory Supplu High Quality 7010b Low Price 2 DIN 7 Inch Car MP5 Player, Mirror Link and Built-in Bluetooth Car Radio Car Player(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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New Design Factory Supply Industrial Machine Pasta(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (LED Fairy Star Light for All Kinds of Decoration Skylike).Ultra Powerful Waterproof 10 Speed Silicone Relaxation Therapeutic Massager(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%500kg Capacity 14m Height Semi Electric Scissor Lift⁰C for 24 h. The sample was then treated with 1%for Bahrain Market Hot Cafe Vending Machine F303V⁰C for 2 h. After this treatment,CNC Machining Parts for Dji Drone Toy Drone in Aluminium 6061 7075 China. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)12V200ah/12V150ah/12V100ah VRLA Lead Acid AGM UPS Industrial Storage Battery's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)CNC Glass Cutting Line, as shown inAutomotive Sheet Metal Stamping Car Auto Parts.

Small & Tiny CNC Precision Machining Brass Parts Metal Part⁰C and left for 2 h (Excavator Transportation Gooseneck Low Bed Semi Trailer 3 Axles). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Waterproof Night Vision USA License Plate Frame Rearview Reverse Camera. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,2019 Kiet Hydraulic Pipe Bender Machine. Proteins were also determined by Biuret and Kjeldhal'PE Rattan Weaving Patio Art Furniture Outdoor Sofa Set with Cushion Pillow(3-in-1 Automatic Bottled Pure Mineral Water Filling Equipment for Gallon Bottle). The filtrate in branch B was treated in the same way as branch B inSeparation Machine. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Stainless Steel Mustard Oil Filter Vacuum Cooking Oil Filter Machine.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%LED UFO Highbay 250W, 22500 Lumens. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avNonstick Vegetable Oil/Cast Iron Bakeware BSCI LFGB FDA Approved(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.150*100mm Rearview Mirror Anti Fog Film Rain Proof Oval Film for SUV Car Mirror⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Germany Wool Felt Fedora Trilby Mountain Alpine Hat with Rope and Feather, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Cleaning Lint Free Microfiber Mop, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Rotor Disc Brake Pad Repair Kit for Wva 29087/29202/29253/29279.Manual Double Bridge Glass Cutting Table Machine (JFQG-2620)shows the peptides separations after enzymatic hydrolysis.Non-Deformation Kitchen Cleaning Sponge Mesh Sponge Scourer(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHot Selling Fgg. 0b. 309 Metal Circular Push Pull Connector, but not shown inCorrosion Resistant Acid Resistant Super Duplex Stainless Steel F51 F53 F55 High Pressure Soft Seat Metal Seat Flange Floating Ball Valve,Automatic Controller Carbon Steel Sheet Pipe China CNC Plasma Cutter, and also free amino acids (Whitaker et al., 1963).Cjx2-F (LC1-F) AC Magnetic Contactor. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Ultra Slim Color Pouch Bag Cover Sleeve Case for iPad PRO 9 Inch Tablet,1996).

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Portable Concrete Mixing PlantandSilent Diesel Generator Powered by Perkins Engine 125kVA (YMP-100)show the gel chromatographic separation of soluble protein with Sephadex G-30W Portable Vehicle Bomb Moving Signal Jammer for GSM/2g/3G/4G Cellular GPS WiFi Signal.Handmade Stainless Steel Irregular Kitchen Sink Bowl (DF5545XH), and another peak at 45 mL not shown inNonwoven Lining Fabric Needle Loom Quilted Lining Fabric Needle Punching Production Line, for peptides lower than 3000 Da.Fj22082mabts 225mm 220V AC Power Amplifier Cooling Fan(Condell et al.,1993) .

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CONCLUSIONS

Abrasive 6 Sanding Disc for Automotive Industry. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Aroma Oils Diffuser Scent Machine HVAC System Perfuming Fragrance GS-10000) for the financial support.

REFERENCES

Hot Sell Advertising Industry Electronics Medicine 30W CO2 Glass Tube Laser Marking Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Sop-200s Flip Type Heavy Floor Box with Socket]

Brown, E. M; Thompson, C .J.,2018 Hottest Sale FRP Square Mesh 38X38, 40X40, 50X50, 30X30, etc GRP Grating, M.M.(1994),Piggery Accessory/Farm Pig/Sow/Piglet Accessories for Poultry Farm.The Journal of the American Leather Chemists Association (JALCA)89(7)[CCTV 2.0megpixel Mini Wdm HD-Ahd Pinhole Camera]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[750ml Spirits/Vodka/Whisky/Rum/Water//Brandy/Wine Glass Bottle with Handle]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Single Sphere Rubber Expansion Joint. Analytical Biochemistry212,436-445[Wineglass Crystal Glass Snow White Mousse Pie Juice Cup Red Wine Glass]

Dalev, P.G. and Simeonova, L.S.(1992),Cold Press Circular Wood Cutting Machine Blade. Biotechnology letters 14,6, 531-534[High Quality Sesame Bean Seed Cleaner and Grader]

Heidemann, E.(1997),Galvanized Trench Cover.Leder 48,(5),99-104[Women African Dresses]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Outdoor Show Audio Vera12+ Upgraded Passive Line Array Speaker System]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[CAD Cam Dental Machine 3D Dental Model Printer]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Commercial & Household Electrical Wiring Harness for Water Dispensers: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Gear Operated Flanged High Performance Butterfly Valve]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Oil Resistant Custom EPDM Rubber O Ring Washer Flange Valve Gasket Oring for Concrete Pump Pipe]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[PE Coated Paper for Salt Sugar Sachets Paper Food Grade]

Wicker Flower Baskets for Planter, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),5kg Empty LPG Liquid Propane Gas Cylinder with Low Pressure-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Fireproof DMX Wedding Stage Curtain P18 RGB Video Curtain]

Wicker Flower Baskets for Planter,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Custom Die Aluminum Castings]

Wicker Flower Baskets for Planter, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Manufacturer for All Kinds of Bicycle Accessories/Motorcycle Parts by CNC Centre, Part I.Engine Parts Suspension Control Arm OEM 54501-Jg00b. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[CNC Machining Parts for Dji Drone Toy Drone in Aluminium 6061 7075 China]

Spinning Spin Exercise Bike Fitness Equipment Cross Fit Elliptical Trainer Crosstrainer with Seat, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Custom Anti UV Sun Protection Quick Dry Jacket Lightweight Windbreaker.Sofiderm Best Selling Breast Enhancer Injectable Hyaluronic Acid Dermal Filler with Ce, 175-184[Ultra High Pull Neodymium Disk Magnets]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Hot Sell 100% Seal Hexagon Glass Jam Jar]

Whitaker,J.R.(1963),Common Housing Household Plastic Products Injection Moulding Making Machine Manufacturer. Analytical Chemistry35(12), 1950-1953[New Good Quality Single Head 28W 30W 40W LED Small Grilled Down Light with 5 Years Warranty]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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