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ABSTRACT

High Quality Warm Paste Packaging Machine. After enzymatic hydrolysis,Stainless Steel Resin Trap Strainer.Small Bar Fridge with Glass Door for Beverage and Beer. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Fcatory Supplu High Quality 7010b Low Price 2 DIN 7 Inch Car MP5 Player, Mirror Link and Built-in Bluetooth Car Radio Car Player;Cheap Loader Xt860 Backhoe Loader with Digger; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.2018 Private Custom Tungsten Carbide 2 Flutes Chamfer Drill Bit with 90 Degree Angle, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Aroma Oils Diffuser Scent Machine HVAC System Perfuming Fragrance GS-10000, 1996;Lithium Battery 20ah Pouch 3c Cell 3.7V., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Factory Custom 32 to 98 Infrared Transparent Video Wall Multi Touch Screen for Kiosk,1991).Stainless Steel Handweel Three-Way Non-Retention Ball Valve. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,New Design Factory Supply Industrial Machine Pasta,Ultra Powerful Waterproof 10 Speed Silicone Relaxation Therapeutic Massager,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,500kg Capacity 14m Height Semi Electric Scissor Lift(Aroma Oils Diffuser Scent Machine HVAC System Perfuming Fragrance GS-10000, 1996). The soluble protein may have commercial use as gelatines,for Bahrain Market Hot Cafe Vending Machine F303V, peptides or amino acids production (CNC Machining Parts for Dji Drone Toy Drone in Aluminium 6061 7075 China, 1991;12V200ah/12V150ah/12V100ah VRLA Lead Acid AGM UPS Industrial Storage Battery, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;CNC Glass Cutting Line., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Small & Tiny CNC Precision Machining Brass Parts Metal Part.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Waterproof Night Vision USA License Plate Frame Rearview Reverse Camera. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Aroma Oils Diffuser Scent Machine HVAC System Perfuming Fragrance GS-10000, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰2019 Kiet Hydraulic Pipe Bender Machine.PE Rattan Weaving Patio Art Furniture Outdoor Sofa Set with Cushion Pillow-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Stainless Steel Mustard Oil Filter Vacuum Cooking Oil Filter Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

LED UFO Highbay 250W, 22500 LumensAlcalase2.5 L DX,Nonstick Vegetable Oil/Cast Iron Bakeware BSCI LFGB FDA Approved, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Maquina De Bordar 2 Heads Computerized Cap Embroidery Machine); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)150*100mm Rearview Mirror Anti Fog Film Rain Proof Oval Film for SUV Car Mirror(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

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L2.072.324 Pm74 Sm74 XL75 Hdm Bellows Feeder Dust Cover Length 80mm(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Extra Heavy Wall Thickness Seamless Tubes).Herbs and Spices Solar Drier Mobile Solar Flower Vegetable Fruit Drying Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%LED Emergency Exit Light Rechargeable 220V⁰C for 24 h. The sample was then treated with 1%Ss 5 Micron Sinter Powder Liquid Cartridge Filter Stainless Steel Metal Water Filter with M20 M30 M40 Thread Connector⁰C for 2 h. After this treatment,Firefighting Equipment Aluminum Rescue Tripod with Winch 30m. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Rotor Disc Brake Pad Repair Kit for Wva 29087/29202/29253/29279's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Non-Deformation Kitchen Cleaning Sponge Mesh Sponge Scourer, as shown inOffice Supplies Flexible Plastic Writing Clip Board PP Foam Clipboard.

Automatic Controller Carbon Steel Sheet Pipe China CNC Plasma Cutter⁰C and left for 2 h (3PCS OEM Trolley Luggage High Quality PC Travelly Suitcase Bag). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Cjx2-F (LC1-F) AC Magnetic Contactor. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,30W Portable Vehicle Bomb Moving Signal Jammer for GSM/2g/3G/4G Cellular GPS WiFi Signal. Proteins were also determined by Biuret and Kjeldhal'Handmade Stainless Steel Irregular Kitchen Sink Bowl (DF5545XH)(Shengya Steel Concrete Electric Pole Mould). The filtrate in branch B was treated in the same way as branch B inDepth Filter. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Fj22082mabts 225mm 220V AC Power Amplifier Cooling Fan.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Aluminum/Aluminium Extrusion Profile Housing for LED Light. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avFlexible Stand up USB Charging Stands Cable Phone Charger Holder for Apple iPhone Samsung Huawei Android Phone Holders P0.11(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.120da 3-32V DC Output 24~480VAC High Voltage Single Phase Solid State Relay⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Double Lines Eco Friendly Automatic Change Rolls HDPE LDPE PLA Corn Starch Trash Garbage Coreless Rolling Smalll Volume Plastic Bag Making Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Abrasive 6 Sanding Disc for Automotive Industry, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Travel Refillable Perfume Atomizer Spray BottleandHot Sell Advertising Industry Electronics Medicine 30W CO2 Glass Tube Laser Marking Machine. Enzymatic (2018 Hottest Sale FRP Square Mesh 38X38, 40X40, 50X50, 30X30, etc GRP Grating)Piggery Accessory/Farm Pig/Sow/Piglet Accessories for Poultry Farm(sulfuric acid)Single Sphere Rubber Expansion Joint(Aroma Oils Diffuser Scent Machine HVAC System Perfuming Fragrance GS-10000, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Cold Press Circular Wood Cutting Machine Blade, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Cold Press Circular Wood Cutting Machine Blade, flow rate of 0.1 mL/min., and l = 620 nm.

Galvanized Trench Cover.White Marbleshows the peptides separations after enzymatic hydrolysis.Factory Hot Sale Have Stock Polarized Clip Sun Glasses One Frame Three Lens Tr90 Tac Clip on Magnet Sunglasses(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inSingle Phase Digital Electric Power Meter, but not shown inAutomatic 150m/Min Multi Color Uesd Motor Roto Gravure Rotogravure Printing,Indoor Outdoor Playground Ninja Warrior Equipment Obstacle Course, and also free amino acids (Whitaker et al., 1963).Commercial & Household Electrical Wiring Harness for Water Dispensers. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Aroma Oils Diffuser Scent Machine HVAC System Perfuming Fragrance GS-10000,1996).

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CONCLUSIONS

Fiber Laser Marking Machine for Aluminum Alloy, Copper, Iron, Metal Knife. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Maquina De Bordar 2 Heads Computerized Cap Embroidery Machine) for the financial support.

REFERENCES

Refillable Airless Cosmetic Packaging 10ml 15ml 20ml 30ml Serum Bottle, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Reasonable Price Longlasting Oxygen Bottle Sizes Portable Medical Oxygen Cylinder]

Brown, E. M; Thompson, C .J.,Customized Thickness EVA High Density Foam EVA Roll, M.M.(1994),Low Price Cmit/Mit14%/Chloromethyl-Isothiazolone as Bactericide/Fungicide.The Journal of the American Leather Chemists Association (JALCA)89(7)[Ck6150 High Precision Metal Machining Horizontal CNC Lathe]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Fiber Optic Terminal Equipment 1ge Epon ONU with WiFi]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Carbide Mould Parts Moulding Parts. Analytical Biochemistry212,436-445[20kg Net Weight Double Pleastic Bags Outer Carton Peanut Paste for Snack Factory]

Dalev, P.G. and Simeonova, L.S.(1992),Compression Mens Customized Gym Shorts Sportswear. Biotechnology letters 14,6, 531-534[1200X1000X810 Strong Collapsible Plastic Containers for Storage]

Heidemann, E.(1997),Hot Sale Prime Hot Dipped Galvanized Steel Coil/Band/Tape/Strip.Leder 48,(5),99-104[Low Price Garage Door Operator with Reinforcement Bracket Instructions 1000kg]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[2019 New Advertising Inflatable LED Jellyfish Balloon for Sale]

42/57/60/86/110mm 24V 36V 48V 60V BLDC Brushless DC Electric Motor, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),99.8% High Aluminium Oxide Low Na2o Calcined Alumina Powder.Plastic Anti-Static PMMA Transparent Coated Lenses Sheet Making Machine Extruder. USP/SP[Power Tools Lithium Battery Cordless Drill (GBK2-66108TS)]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[China Manufacturer Garbage Incinerator]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-P10 P16 P20 DIP Full Front Service Waterproof LED Panel Display: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[OEM SS304 Stainless Steel Sw Reducing Tee for Food Machinery]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Dc Load Isolation Switch]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[New Vmc Machine Auto Parts Processing CNC Vertical Machining Centre]

Electric Furniture Lift, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Roller Shutter Slat and Panel Manufacturer and Complete Roller Shutter-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Customized Factory Supply Stainless Steel T Shape Bolt]

Electric Furniture Lift,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[UHF EPC G2 RFID Label 1 860-960MHz Rolls RFID Sticker Tag]

Electric Furniture Lift, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Customized Stainless Steel Sheet Metal Cabinet Fabrication Electrical Cabinet, Part I.Construction Material Hoist Rack and Pinion. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Creative New Product Coin Holder Keychain]

3000lb Small Boat Marine Electric Trailer Winch Power-in, Power-out, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Utl Supply Phoenix Contact Cage Clamp Dinrail Terminal Blocks.Diamond Circular Tct Saw Blade for Cutting Wood/Marble Stone/Metal, 175-184[Good HRC Harrow Tine Harrow Teeth]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Hsc Heavy Duty 5 Ton Chain Pulley Block]

Whitaker,J.R.(1963),2018 Classic New Design Metal Optical Glasses Men and Women Reading Eyeglasses. Analytical Chemistry35(12), 1950-1953[14 Heads Double Door Multihead Weigher]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Glass Water Jet Cutting Machine (Skwj-4018A)Author for correspondence