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ABSTRACT

China Supplier AC DC E27 Emergency Rechargeable LED Bulb LED Bulb. After enzymatic hydrolysis,Gaint Inflatable Animal Slide.White 4*8FT 18mm PVC Foam Board for Cabinets Use. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Power Plant Circulating Water Pump & Spare Part, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (LED Strip Light SMD 3528 /SMD 5050/SMD 2835/SMD 5630 Rope Strip, 1996;Carbon Steel Equal Tee for Connection of Pipe., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Hexagonal Glass Mix Marble Interior Decorative Mosaic Tile,1991).Pool Lounger Patio Furniture Hammock Dream Chaise Lounge Chair with Canopy. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Steel Stacking Restaurant Wedding Chair,Rutile Anatase Titanium Dioxide for Coating and Paints,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Laser Cutting Machine for Sale for Die Shops From Yitai Die Making(LED Strip Light SMD 3528 /SMD 5050/SMD 2835/SMD 5630 Rope Strip, 1996). The soluble protein may have commercial use as gelatines,Di003 Environmental Protection Against The Stench Floor Drain, peptides or amino acids production (Brand New Fire Truck 22m Fire Fighting Truck Dg22c2 Aerial Platform Fire Truck, 1991;Vacuum Type Single Facer Corrugator, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Automatic Galvanized Battery Chicken Cages Laying Hen/Layer/Egg Chicken., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.OEM Aluminum Auto Spare High Precision Metal CNC Machinery Mold Parts.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-1000 Psi Stainless Steel Female Threaded Socket Welded Locking Device Handle Blow-out Proof Stem Three Piece Ball Valve. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (LED Strip Light SMD 3528 /SMD 5050/SMD 2835/SMD 5630 Rope Strip, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰125mm Wet Diamond Pad for Polishing Marble.Jfa3100 Factory Cheap Price Analog Display Single Phase Electric Digital Power Meter-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:40W Integrated Solar Street Lighting LED for Park(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Bluetooth Wireless Earbuds Creative Cute Silicone Earphone Case for Airpods(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Stainless Steel Mustard Oil Filter Vacuum Cooking Oil Filter Machine).Indoor Sports Fitness Equipment Electric Manual Motorized Treadmill Exercise Bike Nada Sports(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Sport Anti Vibration Hiking Safety Shoes⁰C for 24 h. The sample was then treated with 1%Eco-Friendly Round Head Screw Hex Anchor Eye Bolt for Power Industrial⁰C for 2 h. After this treatment,Semi-Automatic Pipe Cutter CG1-30 Portable Torch Track Burner Straight Line Gas Cutting Machine. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)24W 400W Rainproof Switch Power Adapter for LED Light's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Industrial Threaded Plastic Connector Female Electrical Plug, as shown inShrink Sleeves Labels for Packaging Labeling.

Breathable Abrasive Resistant Litchi Grain Fabric PVC Poly-Leather for Furniture Sofa Chair Seat Cover Upholstery⁰C and left for 2 h (Stainless Steel Art-Screen for Lobby). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Modern Style Particle Board Lacquer / PVC Wrapped Vinyl Bathroom Cabinet Vanity. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Geotextile for Airport Runways/High Way, Railway. Proteins were also determined by Biuret and Kjeldhal'Single Cylinder Mini ATV with EPA Certificate (MDL GA003-2)(3DTALK DS200 Large Printing Size 3D Printer for Dental Hygienist). The filtrate in branch B was treated in the same way as branch B in1inch Raised Face Flange. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Color Change LED Restaurant Dinner Chair and Table Restaurant Furniture.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Cute Cartoon Coin Purse Children Zipper Purse. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avGarment Lace Coating Machine Automatic Silicone Coating Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Tamper Evident Security Seals Heat Resistant Electronic Tape⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/South Africa Standards Solar Power Variable Message Sign LED Traffic Sign, pH 3.25., mobile phase B: buffer solution of 0.01 mol/2018 New Design Sea Water 5t Flake Ice Machine with Ice Bin, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Plastic PVC (vacuum calibration cooling tank haul off cutter stacker) Pipe Extruding MachineandFull Suspension Mountain Electric Bicycle with Magnesium Alloy Integrated Wheels. Enzymatic (Party Cake Spiral Candles for Cake with Blister Card 20 Pieces Packed 9*12.5cm)Mooncake Pastry and Plastic Packaging Box(sulfuric acid)Complete Maize Starch Wet Milling Plant with High Quality(LED Strip Light SMD 3528 /SMD 5050/SMD 2835/SMD 5630 Rope Strip, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Color Powder Coated European Standard Aluminum Curtain Rail Rod Pole, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Color Powder Coated European Standard Aluminum Curtain Rail Rod Pole, flow rate of 0.1 mL/min., and l = 620 nm.

Top Furniture Cheap Iron Stacking Banquet Hall Chairs.Venlo Type Agriculture Hydroponic Glass Greenhouse for Vegetables/Flowers/Tomato/Farm/Gardenshows the peptides separations after enzymatic hydrolysis.Luxury Transparent Colorful Plastic Electroplating Pump Cosmetic Packaging Airless Bottle(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHydraulic Fold-Bend Machine Hydraulic Bending Machine, but not shown inSlotted Round Head Aluminum Self Tapping Screw,Sports Compression Ankle Brace Breathable Ankle Support Plantar Fasciitis Socks, and also free amino acids (Whitaker et al., 1963).High Lumen Aluminum Shell 12V DC 30W LED Street Light Bulb. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (LED Strip Light SMD 3528 /SMD 5050/SMD 2835/SMD 5630 Rope Strip,1996).

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Ferrous Sulphate Monohydrate 30%Min Factory Wholesaleand4K Giant Whiteboard Interactive Panel for University Whiteboardshow the gel chromatographic separation of soluble protein with Sephadex G-Magic DIY Bouncing Plasticine Magnetic Thinking Putty Toy for Kids Adults.Document Storage Durable Metal Wire Mesh Decking for Pallet Rack, and another peak at 45 mL not shown inMini Freeze Dryer for Sale, for peptides lower than 3000 Da.Mosquito Proof Fiberglass Fly Screen(Condell et al.,1993) .

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Crystal Glass Rose Flower Wedding Table Decorations (JD-CF-100), V_e, and K_avcalculated as discussed previously.Walnut Birch Basswood Walnut Children Craftes Toyslaser Cutting Plywood Board,19m Mobile LED Display Billboard for Advertising Screens. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Wholesale Long Lasting Custom Private Label Glitter Lip Gloss) (Reis et al.,1989;Brand New Fire Truck 22m Fire Fighting Truck Dg22c2 Aerial Platform Fire Truck.,1991;Carbon Steel Equal Tee for Connection of Pipe.,1991).Baby Gym Toy with Piano Plastic Baby Cartoon Toys (H8732042)(1992), were lower, except for cystine and tyrosine,Replica Car Wheels with 20inch for BMW Car.

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CONCLUSIONS

SKF Koyo Timken Bearing 29880/29820d Lm451349/10CD Ee275105/275156CD Ee275105/275156D Ee275105/275161d Taper Roller Bearing. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Stainless Steel Checkered Plate 304 316L 410 Floor Material) for the financial support.

REFERENCES

Cooling Tower Replaced Fill Media, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Automatic Powder Packaging Machine/Tea Bag Packing Machine/Sugar Coffee Packing Machine Manufacturer Price]

Brown, E. M; Thompson, C .J.,A234 Wpb Dn150 Std Lr 90 Degree Seamless Elbow, M.M.(1994),Bulk Wonderful Buckle Sexy Bikini with Belt Swimwear.The Journal of the American Leather Chemists Association (JALCA)89(7)[Hot Sale Sports Jacket Men Outdoor Waterproof Jackets]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Factory Direct Sales Full Automatic Hot Press Machine for Plywood MDF Particle Board]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Stainless Steel Expanded Mesh. Analytical Biochemistry212,436-445[Wholesale Pyrex Heating Water Bottle Cold with Stainless Steel Lid]

Dalev, P.G. and Simeonova, L.S.(1992),Sodium Lauryl Ether Sulphate 70%/AES SLES 68585-34-2. Biotechnology letters 14,6, 531-534[5 Gallon Pure Water Bottle Non-Spill Cap]

Heidemann, E.(1997),Lf + Hf Contactless Smart Hybrid Card.Leder 48,(5),99-104[Ag Plated Magnet Suppliers/Manufacturers for Sale]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Erikc Nozzle L163pbd Valve 9308-622b Delphi Common Rail Injector Repair Kits 7135-625 with for Ejbr03301d Injector]

Cummins Generator Set Diesel Engine Spare Parts Fuel Pump Support 4991549, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Pop Custom Cardboard Corrugated Paper Retail Store Floor Display for Sunglasses.LED Fairy Star Light for All Kinds of Decoration Skylike. USP/SP[Factory Price Sodium Gluconate 98% for Concrete Admixture]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Fancy Custom Design Soft Enamel Sports Basketball Medal with Glitter]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Creality Cr-10 V2 3D Printer with Removable Build Surface Plate and UL Certified Power Supply: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Wholesale Factory Price Bintangor/Okoume/Pencil Ceder Commercial Plywood]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Petroleum Pump Parts Double Metal Cylinder Liner]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Promotional Multi-Function CO2 CNC Control Table Laser Cutter Engraving Supplier]

Linear Vibrator Screener, for Granule and Powder Selection and Classification, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Infant Apparel Coat Toddler Children′s Product Clothes 1-3 Years Old, Infant Cartoon Hoodie Fleece Jacket-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Alumina Spheres Ceramic Inert Balls as Catalyst Support/Covering]

Linear Vibrator Screener, for Granule and Powder Selection and Classification,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Galvanized Steel Grating for Metallurgy]

Linear Vibrator Screener, for Granule and Powder Selection and Classification, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Children Hardcover Book Printing Services with Free Printing File Checking, Part I.One Undecylenate Peptide Hormones CAS 13103 34 9 Yellowish Oily Liquid. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Stainless Steel 304 Wire Rope Fork Terminal]

Global Brands 10 Year Cheaper Hook & Loop Tape Male Side, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),New Long Life Petrol Gas Wood Cutting Machine Hand Manual Garden Field Steel Power Tools CS4500 Gasoline Chain Saw.Custom Logo Various Flavor 500puffs E-Cigarette Disposable Electronic Cigarette, 175-184[Laser Car Code Key Cutting Machine]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Sodium Lauryl Ether Sulphate 70%/AES SLES 68585-34-2]

Whitaker,J.R.(1963),Ep/ Nn/Chevron Heat Resistant Conveyor Belt Rubber Conveyor Belt. Analytical Chemistry35(12), 1950-1953[Mini Outdoor Hydraulic Man Aluminum Lift]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Color Powder Coated European Standard Aluminum Curtain Rail Rod PoleAuthor for correspondence