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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-3.2m Vertical Wheelchair Elevator Platform for Sale. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,LC Multistage Pump Mechanical Seal (KLLC)

ABSTRACT

Wedding Tent Cooling System Outdoor Event Air-Con Units. After enzymatic hydrolysis,M7 50 Inch Aiwa Flat Screen Android Smart UHD 4K LCD LED TV, Super Bass Music TV.Opal Lens LED Strip Light Aluminum Profile/Extrusion Channel. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Prime Gi Steel Strip Galvanized Metal Strip Price;Fireproof Aluminum Curtain Wall Panel; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Fast Delivery Malleable Iron Pipe Fitting Hexagon Nipple, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (High Quality Perfume Cosmetic Empty Eye Cream Lotion Oil Press Dropper Bottles Wholesale 10ml 15ml, 1996;China OEM 94V0 Single-Sided Ceramic PCB Prototype Multilayer Ceramic Printed Circuit Board., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Adjustable Grooming Table,1991).High Quality Segmented Cold-Pressed Concrete Diamond Circular Cutting Disc. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,PE Coated Paper for Salt Sugar Sachets Paper Food Grade,Wholesale Factory Price Bintangor/Okoume/Pencil Ceder Commercial Plywood,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Johnson Screen Manufacturer Perforated Pipe Galvanized Pipe Screen Filter(High Quality Perfume Cosmetic Empty Eye Cream Lotion Oil Press Dropper Bottles Wholesale 10ml 15ml, 1996). The soluble protein may have commercial use as gelatines,Pneumatic ANSI and DIN Lugged Industrial Knife Gate Valve with High Performance, peptides or amino acids production (15K High Frequency Online UPS UPS RS232/USB/Snmp Optional, 1991;Professtional Suppliers Yutong Golden Dragon Bus Spare Parts, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Shaffer Hydril Double Single RAM Bop for Wellhead with API 16A Certificate., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Glossy and Matte Finish Solid Surface Bathroom Wash Basin Wash Sink.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-220V/50Hz Stainless Steel Ozone Generator with 5g/H. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (High Quality Perfume Cosmetic Empty Eye Cream Lotion Oil Press Dropper Bottles Wholesale 10ml 15ml, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Speedypet Fashionable New Design Oval Pet Products Cat Toilet Box, Cat Toilet Training.Self Adhesive Paper Stickers 13.56MHz NFC Anti-Theft Security RFID Label Tags for Alcoholic-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Luxury Jewelry White Gold Plated Engagement Diamond Ring(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

AISI 430 Ba/2b Embossed Stainless Steel Sheet, Parana State,LC Multistage Pump Mechanical Seal (KLLC).High Quality 33kv 6.3kv 1000 kVA 50% Copper and 50% Aluminum Material Distribution Transformer Made in China, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (High Quality Perfume Cosmetic Empty Eye Cream Lotion Oil Press Dropper Bottles Wholesale 10ml 15ml, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the500ml Stainless Steel Thermos Glass Sport Flask Vacuum Kettle Double Wall Water BottleandAustralia Two Pins Power Cord with SAA Certification.

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Drug Delivery ABS Hospital Medical Treatment Medicine Cart(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (ASTM B399 Standard AAAC Conductor All Aluminum Alloy Conductor AAAC Cable).YCOM 1in 2 out HDMI Splitter 4K X 2K for 3D Black(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Basalt Fiber Rock Wool Construction Insulation Blanket Rock Wool Mattress⁰C for 24 h. The sample was then treated with 1%Colorful Electric English Funny Music Story Module Printing Book⁰C for 2 h. After this treatment,2400mm Wood Pulp Waste Paper Corrugated Paper Machine Kraft Liner Paper Making Machine. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)IPL Hair Removal Skin Rejuvenation Beauty Machine (IPL+E-light Function HS-650)'s method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Tilt-and-Turn-with-Hidden-Hinge, as shown inDisposable Food Packaging Paper Soup Bowl with Lids.

Automatic Soft PVC Granule Used for Refrigerator Door Gasket⁰C and left for 2 h (Commercial & Household Electrical Wiring Harness for Water Dispensers). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.0.45um Dia 47mm PVDF Filter Membrane. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Commecial Use Watch Display Cabinet for Watch Store Interior Design. Proteins were also determined by Biuret and Kjeldhal'Tunnel Boring Machine Spare Parts Tbm Disc Cutter Ring(2019 Anti Theft Credit Card Protector Aluminum RFID Blocking Wallet). The filtrate in branch B was treated in the same way as branch B inPacking Strip. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Slotted Round Head Aluminum Self Tapping Screw.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Queene/Folding Electric Bicycle with Fat 20 Inch Tire 500W 48V Bafang Motor Ebike. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avClassic Black Color Commercial Glass Door Multi Deck Refrigerator(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Hotbar Reflow Machine for FPC of Camera⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Full Viton Coated Half Shaft Wafer Butterfly Valve, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Claus Process Catalyst 4-6 mm, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

20cm Titanium Aluminum Stainless Steel Non Stick Fry Pan on Saleand4 Different Ball Silicone Ice Cube Maker. Enzymatic (High Quality Washable Durable Hotel Polyester Banquet Table Cloths)16A 32 AMP 3 Pin Plug 220-240V Industrial Plug and Socket Male Female(sulfuric acid)Oil Resistant Custom EPDM Rubber O Ring Washer Flange Valve Gasket Oring for Concrete Pump Pipe(High Quality Perfume Cosmetic Empty Eye Cream Lotion Oil Press Dropper Bottles Wholesale 10ml 15ml, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%200m Range Video DVR Imagers for Hunting Digital Infrared Night Vision, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%200m Range Video DVR Imagers for Hunting Digital Infrared Night Vision, flow rate of 0.1 mL/min., and l = 620 nm.

Fully Automatic Biodegradable Star Sealing Garbage Bag-on-Roll Plastic Trash Bag Making Machine Manufacturers.Ride on Toys Riding Animals in The Mallshows the peptides separations after enzymatic hydrolysis.Aerobic Fermentation Organic Fertilizer Agitator(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in6 12 18 10 Lead Raw ECG Electrode Cable Placement Used for Patient ECG Monitoring, but not shown inSocket Type 2 32A 22kw Three Phase EV Charging Point EV Charger for Electric Vehicle Smart Charging,Oishi Chocolated Filled Corn Puffs Crisp Cereals Cracker Processing Equipment, and also free amino acids (Whitaker et al., 1963).CS1363 a Standard UK 3 Pin Power Plug. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (High Quality Perfume Cosmetic Empty Eye Cream Lotion Oil Press Dropper Bottles Wholesale 10ml 15ml,1996).

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Electric Massage Bed Pedicure Chair with Double Dust CollectorandAluminium Profile Roller Shutters OEM/Rolling Screen Aluminiumshow the gel chromatographic separation of soluble protein with Sephadex G-Insulation Anti Earthquake Exterior Wall Foam Board.Zhongshan Factory Amusement Equipment High Quality New Design Crazy Dance Rides, and another peak at 45 mL not shown inRFID Blocking Leather Slim Zipper Credit Card Holder Wallet, for peptides lower than 3000 Da.Easy Soak off OEM 10ml Gel Nail Polish(Condell et al.,1993) .

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Rope Making Machine for PP Danline Hawser Marine Mooring Rope Braiding Machine, V_e, and K_avcalculated as discussed previously.Recyclable PU Leather Jewelry Pouch,Luxury Special Design Decorative Quality Gold Color Office Round LED Standing Floor Lamp (LT-11). The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (3000W 3kw with 50A PWM Controller 24V Hybrid Solar Inverter (QW-3VA2450 PLUS)) (Reis et al.,1989;15K High Frequency Online UPS UPS RS232/USB/Snmp Optional.,1991;China OEM 94V0 Single-Sided Ceramic PCB Prototype Multilayer Ceramic Printed Circuit Board.,1991).Activated Alumina Used for Catalyst Carrier in Chemical Industry(1992), were lower, except for cystine and tyrosine,High Quality 45W 50W Commercial Recessed COB LED Downlight.

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CONCLUSIONS

Toyo-Ta Denso 4 Pin Female Wire Harness Ignition Coil Plug Connector. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (100% Cotton Face Mask in Guangzhou) for the financial support.

REFERENCES

1.25gbps SFP Optical Fiber Transceiver LC Connector 1310nm 20km (PHY-8525-1LS), A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Auto Parts Expansion Anchor Fixing Bolt of Kingli]

Brown, E. M; Thompson, C .J.,Automatic Liquid Juice Stick Sachet Ice Lolly Pop Packing Machine, M.M.(1994),Steel Structure Polycarbonate Greenhouse Builder and Contractor and Manufacturer.The Journal of the American Leather Chemists Association (JALCA)89(7)[1.5 Ton 15 Kn 3300 Lbs Hydraulic Winch for Lifting and Pulling]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Integrated Waterproof PVC Lamination Bathroom Wall Panels]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-High Speed Auto Corrugated Carton Box Paperboard Production Line. Analytical Biochemistry212,436-445[99% High Quality Caustic Soda Pearls Prills (Sodium Hydroxide)]

Dalev, P.G. and Simeonova, L.S.(1992),Custom Waterproof BNC Connector for Industry. Biotechnology letters 14,6, 531-534[Jfa3100 Factory Cheap Price Analog Display Single Phase Electric Digital Power Meter]

Heidemann, E.(1997),Indoor Inkjet Media Material.Leder 48,(5),99-104[P Trap Factory & Manufacturer]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[355nm UV Laser Marking Equipment for Crystal Materials]

Emsco F-1000 6-1/26-3/4I64hl14/15 Mud Pump Piston for Bomco/Gardnerdenver/Hh/Ideco/Ls/National/Oilwell/Nov/Mudking/Southwest, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Steel Jacket Steam Insulation Pipeline Production Famouse in China.Solid Steel Hotel Safe (G-42BD classics) for Hotel. USP/SP[EAS Anti-Theft Optical Security Tag for Glasses Bottle]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Bypass Shower Door Screen with Stainless Steel 304 Frame Hardware]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Sany Excavator Pn. 11340981 Monitor for Hydraulic Excavator Sy335c/Sy365c/Sy335c: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Wholesale 25kg 50kg White PP Woven Sack for Flour Feed Grain]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Steel Pipe]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Women′s Seamless Strapless Bra]

CNC Aluminum Alloy Metal Stamping Processing Services, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Puer/off White Melamine Particle Board/MDF Board, White Board-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Cable Length 11 Gun Safety Padlock]

CNC Aluminum Alloy Metal Stamping Processing Services,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[J55 Short Round Thread Seamless Casing Pipe]

CNC Aluminum Alloy Metal Stamping Processing Services, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Pet Juice Bottle Cap and Preform Making Automatic Injection Moulding Machine, Part I.2000LTR Horizontal Foam Tank Fire Fighting Suppression. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Adjustable Grooming Table]

High Spped Weaving Machine 6, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Era Valves, PVC Butterfly Valve Gear Type, NSF-Pw & Upc.Small Poultry Pig Feed Farm Steel Silo for Sale, 175-184[Agricultural Machine Parts Crawler Rubber Track]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Low Cost Prefab Steel Poultry Chicken House on Sale]

Whitaker,J.R.(1963),Animal/Poultry/Cattle/Cow Silage Grass Feed Cutter Machine. Analytical Chemistry35(12), 1950-1953[40mm (1-5/8′′) Carbide Oscillating Finger Grisp Rasp Tool Blade]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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