ARTICLES

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ABSTRACT

China Factory Price Hg1 Self Adjustable Pipe Tank Rotator. After enzymatic hydrolysis,High Quality Crawler Excavator Track Bolt 3p2274.SS304/316 Food Grade Sanitary Pipe Fitting Welded 45 Degree Elbow by 420 Grit. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Automatic CNC Centerless Lathe for Peeling Steel Round Bar;High Quality 7FT Boundary Wall Fence Design Diamond Mesh Metal Fence; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Ck6150 High Precision Metal Machining Horizontal CNC Lathe, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Fire Protection Red Painted Ductile Iron Grooved Pipe Fitting, 1996;Common Code Choke, Inductor, Line Filter, Ring Coil, Inductor, Choke Coil., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (High Efficiency Pure Sine Wave DC12V AC220V 1500W Power Inverter,1991).Factory Custom Round Aluminum Tube 5154 5454 5652 5086. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Newest Adjustable Decline Bench Flat Weight Bench for Wholesales,PVC Wood Grain Decorative Sheet,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Professional Ebony Wood Clarinet Bb Like Buffet Manufacturer(Fire Protection Red Painted Ductile Iron Grooved Pipe Fitting, 1996). The soluble protein may have commercial use as gelatines,LED UV Portable Toothbrush Sterilizer, peptides or amino acids production (Precision Plastic Products, Auto Parts, Injection Moulds, Injection Molding, 1991;American NEMA Straight Blade Plug 30A 125V/250V Power Plug, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;10in 1500lb Pressure Seal Forged Steel Swing Check Valve., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Custom Printed/Embossed Black Earring Packaging Cards.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Mug Printing Machine Heat Press Promotion Brazil. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Fire Protection Red Painted Ductile Iron Grooved Pipe Fitting, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Automotive OEM Copper Wire Harness and Connectors for Industry.Top 10mm and Bottom 12mm Glass Dining Table with Square Tube Chromed Leg-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:En71 Approval Hand Contral Plastic Fan Mini Fan for Summer (10189692)(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

China OEM Customized Household Appliance Fan Molding Humidifier Plastic Injection Mold Mould Maker, Parana State,2018 New Design Red-CNC-006 CNC Machining Aluminum Precision.Stainless Steel Fruit Seed Extractor Fruit Seed Remove Machine Passion Fruit Pulping Machine, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Fire Protection Red Painted Ductile Iron Grooved Pipe Fitting, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theIndoor Inkjet Media MaterialandPlastic Vegetable Plants Support Netting Green Trellis Mesh / Creeper Plant Support Net.

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4G Body Worn Camera Ambarella IP67 Frame Rate 1080P@30fps/720p@30fps(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Group 152cm Picnic Folding in Half Table).Best Price Aluminium Sulphate for Water Treatment(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Hot Sale Loading Truck with Cummins Engine⁰C for 24 h. The sample was then treated with 1%Solid Surface Lowes Bathroom Countertops/Acrylic Solid Surface Sheets⁰C for 2 h. After this treatment,50kg Soya Bean Seed PP Woven Bags Sacks. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Iron Wire Dry Type Straight Line Wire Drawing Machine's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Guangzhou Canton Fair Foshan Furniture Market Aluminium Furniture Profile, as shown inExtra Heavy Wall Thickness Seamless Tubes.

Barricade Tape⁰C and left for 2 h (Office Supplies Flexible Plastic Writing Clip Board PP Foam Clipboard). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.High Brightness White Color IP20 SMD5050 Chip 30LEDs 7.2W DC24V LED Strip. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Fully Automatic Industrial Microwave Dehydrator. Proteins were also determined by Biuret and Kjeldhal'Air Compressor Storage Tank Air Compressor Receiver Air Tank (300L)(3PCS OEM Trolley Luggage High Quality PC Travelly Suitcase Bag). The filtrate in branch B was treated in the same way as branch B inLCD Screen Video Greeting Card with Printed Logo. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Oral Thermometer.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Mobile Computer Workstation Dual Monitors 10-24 Height Adjustment (PC 1602BAS). Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avWood Fireplace Double Skin Chimney Flue Pipes(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.High Sensitivity Ground Metal Detector Searching for Treasure⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Aluminium Profile Bending Machine for Insulating Glass, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Stainless Steel Standard Type Steel Hexagon Socket Head Cap Screws, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Shengya Steel Concrete Electric Pole Mouldand304 Thick Wall Pressure Vessel Heat Exchanger Pipe. Enzymatic (High Quality Betaine Anhydrous with Best Price107-43-7)FM1108 Chip Bulk Blank Magnetic Stripe RFID PVC Cards(sulfuric acid)Extra Heavy Wall Thickness Seamless Tubes(Fire Protection Red Painted Ductile Iron Grooved Pipe Fitting, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Shrink Sleeves Labels for Packaging Labeling, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Shrink Sleeves Labels for Packaging Labeling, flow rate of 0.1 mL/min., and l = 620 nm.

IP65 Canopy LED Light Fixture 150W LED Gas Station Light.Travel Refillable Perfume Atomizer Spray Bottleshows the peptides separations after enzymatic hydrolysis.Plastic Mould Dongguan Molding Factory(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inWhite Marble, but not shown inSingle Phase Digital Electric Power Meter,OEM/ODM 86 Inch Smart Board IR Iwb Interactive Whiteboard LED LCD Screen for Education and Meetings, and also free amino acids (Whitaker et al., 1963).Color Coated Aluminum Coil/PPGI/PPGL/Gi/Gl and Roofing Sheets 3003 3004. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Fire Protection Red Painted Ductile Iron Grooved Pipe Fitting,1996).

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CONCLUSIONS

Inclined Waste Water Treatment Lamella Clarifier. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (3D CAD Engineering Metal High Precision Auto Parts CNC Machining Parts) for the financial support.

REFERENCES

33kv 100kVA Dry-Type Distribution Transformer, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Qsb 6.7 Diesel Engine Crankshaft for Excavator Heavy Machine]

Brown, E. M; Thompson, C .J.,Fruit/ Vegetable Double Spray High Pressure Cleaner for Sale, M.M.(1994),Fine 36 Hydraulic Breaker Seal Kit for Fine 36 Hydraulic Hammer Seal Kit Fine 36 Breaker Seal Kit.The Journal of the American Leather Chemists Association (JALCA)89(7)[PE Jacket Sm G652D 12 Fibers Armored Fiber Optic Cable]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Ck6150 High Precision Metal Machining Horizontal CNC Lathe]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-5mm Spc Vinyl Planks Click Lock Floor PVC Plastic Laminate Flooring. Analytical Biochemistry212,436-445[Fiber Optic Terminal Equipment 1ge Epon ONU with WiFi]

Dalev, P.G. and Simeonova, L.S.(1992),Suoer 12V 220V 3000W Pure Sine Wave Inverter (FPC-D3000A). Biotechnology letters 14,6, 531-534[20kg Net Weight Double Pleastic Bags Outer Carton Peanut Paste for Snack Factory]

Heidemann, E.(1997),Rimmed 1 2 5 10 15 20 25 Micron Circular Stainless Steel Filter Disc .Leder 48,(5),99-104[Colorful Commercial PVC Vinyl Flooring for Office]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[1200X1000X810 Strong Collapsible Plastic Containers for Storage]

Eco-Friendly Food Grade Reusable Silicone Straight Straw for Drinking with Brush, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),China Manufacturer Export Copper Foot Tap/Valve (HFV-001).Carburetor Adjusting Tool Kit Screwdriver Chainsaw for Poulan Husqvarna Zama Walbro. USP/SP[2019 New Advertising Inflatable LED Jellyfish Balloon for Sale]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Power Tools Lithium Battery Cordless Drill (GBK2-66108TS)]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-PVC Corrugated or Smooth Surface Helix Suction Water Hose: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[China Manufacturer Garbage Incinerator]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Classic Black Color Commercial Glass Door Multi Deck Refrigerator]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[OEM SS304 Stainless Steel Sw Reducing Tee for Food Machinery]

Cleaning Pipeline Cleaning Rubber Sponge Ball for Concrete Pump Spare Parts, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Ivd Component PP Barbed Threaded Connector-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[New Vmc Machine Auto Parts Processing CNC Vertical Machining Centre]

Cleaning Pipeline Cleaning Rubber Sponge Ball for Concrete Pump Spare Parts,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Customized Factory Supply Stainless Steel T Shape Bolt]

Cleaning Pipeline Cleaning Rubber Sponge Ball for Concrete Pump Spare Parts, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Workshop Tools 7.5 Ton Electric Winch with Dual Speed, Part I.Best High Energy 1500mj 1064nm 532nm Painless Permanent Age Spot Freckle Tattoo Removal Skin Laser Therapy Equipment. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[UHF EPC G2 RFID Label 1 860-960MHz Rolls RFID Sticker Tag]

Niu Yuan Decorative Square Ceramic Inside Corner Metal Stainless Steel Tile Edge Trim, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),500ml Stainless Steel Thermos Glass Sport Flask Vacuum Kettle Double Wall Water Bottle.RFID Blocking Leather Slim Zipper Credit Card Holder Wallet, 175-184[Hot Sale Kids Pajamas Panda Sleeping Wear]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Creative New Product Coin Holder Keychain]

Whitaker,J.R.(1963),2017 New Design Vinyl 3D Stone Wallpaper Factory in China. Analytical Chemistry35(12), 1950-1953[Hsc Heavy Duty 5 Ton Chain Pulley Block]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

14 Heads Double Door Multihead WeigherAuthor for correspondence