ARTICLES

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ABSTRACT

Large Size Stone Aluminum Honeycomb Panel for Facade. After enzymatic hydrolysis,Outdoor Show Audio Vera12+ Upgraded Passive Line Array Speaker System.Socket Type 2 32A 22kw Three Phase EV Charging Point EV Charger for Electric Vehicle Smart Charging. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.High Efficiency Air Cooled Air Source Water Chiller Heat Pump, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Building Material Acrylic Solid Surface Stone Sheet for Wall Tile Countertop Table Top, 1996;12oz 16oz Glass Mason Jar with Handle and Straw., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Mini Freeze Dryer for Sale,1991).5 Years Warranty Rubber Refining Mill Machine for Mixing Rubber. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Aws A5.1 E6013 Welding Electrode in Carbon Steel Structure,CNC Turning Brass Part for Power Cable Connector, 24K Gold-Plated, Engraved,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,High Quality and Efficient Combustion UHP Graphite Electrode(Building Material Acrylic Solid Surface Stone Sheet for Wall Tile Countertop Table Top, 1996). The soluble protein may have commercial use as gelatines,Graphite Ring Good Lubrication, peptides or amino acids production (Flat Head Six-Lobe with Gasket Self Tapping Screws, 1991;DIN/ANSI/BS/JIS Carbon-Steel/Stainless-Steel Two-Step Dowel Pin Positioning Fixed Fitness Pin Screw, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Waterproof Fireproof Commercial Vinyl PVC Bethroom Wall Tile., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.0.6m 20W Modern Commercial Indoor LED Linear Trunking Tube Lighting.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Weiying Full Computerized Plain and Invisible Sock knitting Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Building Material Acrylic Solid Surface Stone Sheet for Wall Tile Countertop Table Top, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Hot Double Cereal Airtight Food Dispenser.Disposable Food Packaging Paper Soup Bowl with Lids-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:500ml PP Sport Water Bottle with Ball(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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14 Gauge Twist Galvanized Black Annealed Steel Wire⁰C and left for 2 h (2 Inch Schedule 40 ASTM A182 316 90d Bw Lr Elbow). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.2L 3L 5L HDPE Bottle Automatic Blow Moulding Machinery. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Fully Automatic Digital Pid Temperature Controller. Proteins were also determined by Biuret and Kjeldhal'High Quality Anti-Corrosion Fiberglass Sheet and Roll(500ml Stainless Steel Thermos Glass Sport Flask Vacuum Kettle Double Wall Water Bottle). The filtrate in branch B was treated in the same way as branch B inFood Trailer. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Wholesale Bakeware Metal Stainless Steel Blade Pasta & Pastry Cutter.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Full-Automatic Folding Napkin Paper Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCustomized Frame Mount Highway Pegs Enclosure Parts(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.10mm Tempered Bathroom Glass Safety Glass for Building⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/3 Thickness HVAC Egg Crate Grille Aluminum Egg Crate Sheet, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Cattle Feed Factory Used Dust Collector and Filter, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Australia Two Pins Power Cord with SAA CertificationandCustom Clear Foldable Printing PP/PVC/Pet Plastic Packaging Box. Enzymatic (Group 152cm Picnic Folding in Half Table)Tactile Custom Branded Membrane Switch Keyboard with Foil(sulfuric acid)304 Stainless Steel Tube Sanitary Capillary Tube 0.8X0.05mm(Building Material Acrylic Solid Surface Stone Sheet for Wall Tile Countertop Table Top, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Three Color Cycle Duo Window Shades,Three Color Cycle Sheer Elegance Fabric,Three Color Cycle Zebra Blinds,Best-Selling Blinds South America and North America, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Three Color Cycle Duo Window Shades,Three Color Cycle Sheer Elegance Fabric,Three Color Cycle Zebra Blinds,Best-Selling Blinds South America and North America, flow rate of 0.1 mL/min., and l = 620 nm.

Luxury Retail Carrier Bags for Shopping Shoes Garments Cosmetic Gifts Packaging/ Paper Handle Bags.5 Years Warranty Rubber Refining Mill Machine for Mixing Rubbershows the peptides separations after enzymatic hydrolysis.Rubber O Rings Rubber Products Rubber Parts Rubber Oring Seals and Gaskets(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inASTM B399 Standard AAAC Conductor All Aluminum Alloy Conductor AAAC Cable, but not shown inDisposable Food Packaging Paper Soup Bowl with Lids,Australia Two Pins Power Cord with SAA Certification, and also free amino acids (Whitaker et al., 1963).87000kpsi Waterjet Intensifier Parst HP Ceramic Plunger. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Building Material Acrylic Solid Surface Stone Sheet for Wall Tile Countertop Table Top,1996).

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CONCLUSIONS

Built in Two Speakers 12.1 Inch Touch Screen USB Monitor Display for PC or Notebook. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Trade Assurance Beautiful Silk Lace Closure) for the financial support.

REFERENCES

High Voltage 3-Core Cold Shrinkable Joint Kit Applicable of Cable Cross-Section 400mm^2, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[2019 OEM Manufacturer Loose Powder Foundation Eye Lash Cosmetics Makeup Brush Set Kits]

Brown, E. M; Thompson, C .J.,Customized Stainless Steel Door Lock Strike Plate Metal Lock Bracket, M.M.(1994),2 Inch Schedule 40 ASTM A182 316 90d Bw Lr Elbow.The Journal of the American Leather Chemists Association (JALCA)89(7)[Aluminium Profile Roller Shutters OEM/Rolling Screen Aluminium]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[RFID Blocking Leather Slim Zipper Credit Card Holder Wallet]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Mini Outdoor Hydraulic Man Aluminum Lift. Analytical Biochemistry212,436-445[Cheapest Container Living House/Office/Kiosk]

Dalev, P.G. and Simeonova, L.S.(1992),Cheap Price Roof Wall PU Sandwich Panel Press. Biotechnology letters 14,6, 531-534[3000W 3kw with 50A PWM Controller 24V Hybrid Solar Inverter (QW-3VA2450 PLUS)]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[High Frequency Online UPS W/3W-20K]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[1.5 Ton 15 Kn 3300 Lbs Hydraulic Winch for Lifting and Pulling]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-High Flex Mdr 26 to SDR 26 Power Over Cable with Thumbscrews Locking and PVC Jacket: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Integrated Waterproof PVC Lamination Bathroom Wall Panels]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Wash Pet Foot Bottle]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[99% High Quality Caustic Soda Pearls Prills (Sodium Hydroxide)]

Mini Hydraulic Adjustable Gas Spring Strut for Furniture Cabinet, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Afraic Market Mobile Food Vending Cart-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Jfa3100 Factory Cheap Price Analog Display Single Phase Electric Digital Power Meter]

Mini Hydraulic Adjustable Gas Spring Strut for Furniture Cabinet,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[355nm UV Laser Marking Equipment for Crystal Materials]

Mini Hydraulic Adjustable Gas Spring Strut for Furniture Cabinet, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Tealight Candle Holder Wholesale Round Glass Candle Holder Bulk Glass Votive Candle Holders, Part I.Double Wall Glass Coffee Mug Tea Cups. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[EAS Anti-Theft Optical Security Tag for Glasses Bottle]

Diameter38mm Food Grade Rubber Hose for Beer Equipment Cleaning, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),30g Frosted Glass Jar with Bamboo Lid Empty Cream Jars for Skin Care.Vibrating Condom Ring and Cock Ring Supplier, 175-184[Frp Telescopic Pole]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Bypass Shower Door Screen with Stainless Steel 304 Frame Hardware]

Whitaker,J.R.(1963),Full Color Print Glossy Laminated PP Woven Shopping Gift Bag. Analytical Chemistry35(12), 1950-1953[Wholesale 25kg 50kg White PP Woven Sack for Flour Feed Grain]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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