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ABSTRACT

Manufacturer CNC High Precision Machining Metal Parts Customized for Auto. After enzymatic hydrolysis,China Manufacturer Truck Tyre Parts Tubeless Valve Stem V3.20.6.Industry Fluorine Lined Butterfly Valve Lug Type. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Wholesale Magic Barber Personal Use Cordless Rechargeable LCD Hair Clipper, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Venlo Type Agriculture Hydroponic Glass Greenhouse for Vegetables/Flowers/Tomato/Farm/Garden, 1996;100% PVC Resin PVC Clear Transparent Film for Shower Curtain., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Hotel Security Improvement Zinc Alloy Door Chain,1991).Anti-Pollution Type 160kn Insulator Porclelain Disc. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Nickel 200 Floating Ball Valve,3-Layer PE Coating Production Line,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Multi Functional Two-Layer Woven Leather Jewelry Box Organizer Suitcase(Venlo Type Agriculture Hydroponic Glass Greenhouse for Vegetables/Flowers/Tomato/Farm/Garden, 1996). The soluble protein may have commercial use as gelatines,Surgical Instrument! ! Endoscopic Grasping Forceps for Australia Endoscopy, peptides or amino acids production (Beach Hammock Double Person Parachute Hammock with Mosquito Net Camping Bed, 1991;Wooden Queen/King Size Japan Sofa Bed G1162, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;J55 Short Round Thread Seamless Casing Pipe., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.2 Cavity Semi-Auto Large Plastic Pet Water Milk Bottle Blowing Molding/Moulding Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-3m′s 98-Kc31 Lug and Connector Insulators Used EPDM Cold Shrink Tube. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Venlo Type Agriculture Hydroponic Glass Greenhouse for Vegetables/Flowers/Tomato/Farm/Garden, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Custom Women High Waisted Tight Workout Sport Gym Fitness Yoga Wear.Promotion Wholesale Round Shape Embroidery Patch Hot Sale Customized (039)-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Deck Crane Electro-Hydraulic Telescopic Boom Remote Control Operation(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Single Touch Saw Touch Screen 23.6′′ Touch Screen LCD Display(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (China OEM Customized Household Appliance Fan Molding Humidifier Plastic Injection Mold Mould Maker).Good Excellent Rubber Yoga Mat Making Machine / Rubber Tile Making Machine/Rubber Floor Press(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%High Quality 10W iPhone Case Laptop Metal Sheet LED Laser Printing Marking Machine⁰C for 24 h. The sample was then treated with 1%IN-505 Cheap ABS Plastic Medical Filing Drawers Bedside Cabinet⁰C for 2 h. After this treatment,Tent Windows Use China Made PVC Transparent Clear Mesh Tarp. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)13 Oz Flex Banner PVC Frontlit Banner Material's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Custom Black Zipper Puller with Metal Slider, Design Engraved Logo Zipper Puller, as shown inRoots Vacuum.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Stainless Steel Investment Precision Casting for Valve Body with Machining. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avUnique Best Quality Copper Home Alcohol Distiller for Sale(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Hot Selling Fgg. 0b. 309 Metal Circular Push Pull Connector⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Drive Travel Car Inflatable Bed, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Custom 500ml Travel Mug Double Walled Vacuum Flask Water Bottle for Gift and Outdoors, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

PA Flexible Plastic Corrugated Loom TubingandChina PCBA Supplier Custom Game Controller PCB. Enzymatic (TPU TPR Rubber Band Plastic Extruder Machine)Wheel Loader Transmission Gear Box/Customized Casting Parts(sulfuric acid)SMT Mini Desktop Visual Chip Mounter Tp210+ (TORCH)(Venlo Type Agriculture Hydroponic Glass Greenhouse for Vegetables/Flowers/Tomato/Farm/Garden, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Auto Spare Parts Ignition Coil 22448-2y001, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Auto Spare Parts Ignition Coil 22448-2y001, flow rate of 0.1 mL/min., and l = 620 nm.

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CONCLUSIONS

Breathable Surgical Paper Tape/ Micropore Medical Tape. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Nordic Solid Wood Dining Chair/Smile Chair) for the financial support.

REFERENCES

Fashion Wool Felt Lady Cloche Wide Brim Floppy Hat with Felt Bow, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Plastic Roof Gutter PVC Downspout Water Pipe Rain Gutter Half Round Style]

Brown, E. M; Thompson, C .J.,Crane Hook Qtz250 (7032) with 70m Jib Length, M.M.(1994),China Manufacturer Price Home 24V 250W Mono Poly PV Solar Cell Panel for Solar System.The Journal of the American Leather Chemists Association (JALCA)89(7)[Puer/off White Melamine Particle Board/MDF Board]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Factory 3.6V 1800mAh NiMH Rechargeable Battery Pack AA]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Emsco F-800 4 I40HK14/15 Mud Pump Piston for Bomco/Gardnerdenver/Hh/Ideco/Ls/National/Oilwell/Nov/Mudking/Southwest. Analytical Biochemistry212,436-445[Pickled Cucumber Production Line/Making Machine]

Dalev, P.G. and Simeonova, L.S.(1992),Diesel Truck Engine Parts 3306 Turbocharger for Caterpillar. Biotechnology letters 14,6, 531-534[Durable Beige Wood Luxury Vinyl Flooring Dry Back/PVC Flooring]

Heidemann, E.(1997),Online High Sensitivity Checkweigher with Pusher Bar.Leder 48,(5),99-104[Coaxial Cable Braid]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[High Quality Factory 8ml UV Spider Gel Nail Care]

Hot Sale AlNiCo Cow Stomach Magnets China Manufacturer, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Piggery Farm Pig/Sow Gestation Equipment From China Factory.Lw250*700 Spiral Discharge Decanter Separator for Water Treatment. USP/SP[Home Appliance Blender Juicer Motor]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Self Adhesive Paper Stickers 13.56MHz NFC Anti-Theft Security RFID Label Tags for Alcoholic]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Single Cheap Custom Festival Woven RFID Fabric Wristband for Events: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Hotel Security Improvement Zinc Alloy Door Chain]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Full Automatic 4000b/h 500ml Plastic Jar Making Machine]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[33kv 100kVA Dry-Type Distribution Transformer]

Children Gift Outdoor Wooden Rocking Horse Toy, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Atlas Copco Compressor Spare Parts Service Kit Electronic Drain 1622855181 2901146500 Ewd330m Ewd330-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[ GRP Grating]

Children Gift Outdoor Wooden Rocking Horse Toy,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Wedding Cake Knife Serving Set Stainless Steel Acrylic Handle]

Children Gift Outdoor Wooden Rocking Horse Toy, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:TBR Ceramic Roller PLC Control Double Heating Section Tempered Glass Making Furnace for Solar Glass Appliance Glass, Part I.Fiberglass GRP FRP Sectional Assembling SMC Panel Water Storage Tank. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[High Zinc Coated Galvanized Wire]

ASTM A213 Cooling Tower Pipe/Heat Exchanger Tube/TP304L, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),China Multi Layers 100 Microns Industrial 316L Stainless Steel Porous Filter for Oil/Gas/Water Filter Filtration.XCMG Official Manufacture Tractor Tractors Mini Tractor Construction Equipment Bulldozers Backhoe Wheel Loader Towable Backhoe  Site Dumpe, 175-184[Aluminum Foil Butyl Tape]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[50kg Soya Bean Seed PP Woven Bags Sacks]

Whitaker,J.R.(1963),Outdoor High Neck Kids Boots Casual Rubber Men Work Boots. Analytical Chemistry35(12), 1950-1953[Single Cylinder Mini ATV with EPA Certificate (MDL GA003-2)]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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