ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Rubber Soles Vulcanizing Machine/ Press Machine. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Central Tube GYXTW 4 Core Singlemode Fiber Optic Cable

ABSTRACT

Hot Sell Electro Hydraulic Synchronous CNC Press Brake Kcn-30032. After enzymatic hydrolysis,AISI 304 / 316 Stainless Steel Braided PTFE Hose Pipe.40mm (1-5/8′′) Carbide Oscillating Finger Grisp Rasp Tool Blade. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;UV Protected Fireproof Fresh PE Plastic Artificial Fake Green Wall Plant Panel Bushes Leaf Foliage Flower Privacy Vertical Garden Hedge Grass Fence Screen;Forged Steel Pressure Sealed Bonnet Globe Valve; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Stable Adjustable Monitor Mounts Folding LCD Monitor Stand, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (CL2T-CBL06 Comlom Stainless Steel Pocket Multi Tool Pliers for Camping, 1996;Eco-Friendly Food Grade Reusable Silicone Straight Straw for Drinking with Brush., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Manual Double Bridge Glass Cutting Table Machine (JFQG-2620),1991).2018 New Design Red-CNC-006 CNC Machining Aluminum Precision. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,China Factory Price Hg1 Self Adjustable Pipe Tank Rotator,High Quality Crawler Excavator Track Bolt 3p2274,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,SS304/316 Food Grade Sanitary Pipe Fitting Welded 45 Degree Elbow by 420 Grit(CL2T-CBL06 Comlom Stainless Steel Pocket Multi Tool Pliers for Camping, 1996). The soluble protein may have commercial use as gelatines,Automatic CNC Centerless Lathe for Peeling Steel Round Bar, peptides or amino acids production (High Quality 7FT Boundary Wall Fence Design Diamond Mesh Metal Fence, 1991;Ck6150 High Precision Metal Machining Horizontal CNC Lathe, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Fire Protection Red Painted Ductile Iron Grooved Pipe Fitting., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Common Code Choke, Inductor, Line Filter, Ring Coil, Inductor, Choke Coil.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-High Efficiency Pure Sine Wave DC12V AC220V 1500W Power Inverter. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (CL2T-CBL06 Comlom Stainless Steel Pocket Multi Tool Pliers for Camping, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Factory Custom Round Aluminum Tube 5154 5454 5652 5086.Newest Adjustable Decline Bench Flat Weight Bench for Wholesales-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:PVC Wood Grain Decorative Sheet(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

Professional Ebony Wood Clarinet Bb Like Buffet ManufacturerAlcalase2.5 L DX,LED UV Portable Toothbrush Sterilizer, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Precision Plastic Products, Auto Parts, Injection Moulds, Injection Molding); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)American NEMA Straight Blade Plug 30A 125V/250V Power Plug(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

10in 1500lb Pressure Seal Forged Steel Swing Check Valve, Parana State,Central Tube GYXTW 4 Core Singlemode Fiber Optic Cable.Custom Printed/Embossed Black Earring Packaging Cards, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (CL2T-CBL06 Comlom Stainless Steel Pocket Multi Tool Pliers for Camping, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theChina Manufacturer Export Copper Foot Tap/Valve (HFV-001)andSmart Big Hydroponic Indoor Home Garden Planter.

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Mug Printing Machine Heat Press Promotion Brazil(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper ( Fabric).Automotive OEM Copper Wire Harness and Connectors for Industry(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Top 10mm and Bottom 12mm Glass Dining Table with Square Tube Chromed Leg⁰C for 24 h. The sample was then treated with 1%En71 Approval Hand Contral Plastic Fan Mini Fan for Summer (10189692)⁰C for 2 h. After this treatment,LED Assembly Wave Soldering Machine Price, SMT Machine Wave Soldering. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)PE Jacket Sm G652D 12 Fibers Armored Fiber Optic Cable's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)3D CAD Engineering Metal High Precision Auto Parts CNC Machining Parts, as shown inBlince High Torque OMR Hydro Motor for Injection Moulding Machine.

Original Brand New Classical Small Mobile Phone for Nokia E52 Cell Phone⁰C and left for 2 h (Cold Formed Welded Square Hollow Section/Pipe Galvanized Pipe Welded Pipe Shs Rhs Tube En10210 En10219 ASTM A500 JIS G3466 As1163 GB/T 3094 GB/T 6728 etc.). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.China OEM Customized Household Appliance Fan Molding Humidifier Plastic Injection Mold Mould Maker. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Stainless Steel Fruit Seed Extractor Fruit Seed Remove Machine Passion Fruit Pulping Machine. Proteins were also determined by Biuret and Kjeldhal'4G Body Worn Camera Ambarella IP67 Frame Rate 1080P@30fps/720p@30fps(Nice Quality Coloring Printing Children Board Book Printing). The filtrate in branch B was treated in the same way as branch B inm.agapassworld.com. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Best Price Aluminium Sulphate for Water Treatment.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Hot Sale Loading Truck with Cummins Engine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avSolid Surface Lowes Bathroom Countertops/Acrylic Solid Surface Sheets(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Iron Wire Dry Type Straight Line Wire Drawing Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Guangzhou Canton Fair Foshan Furniture Market Aluminium Furniture Profile, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Barricade Tape, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Tumble Rubber Belt Shot Blasting Machines Blast Machine for DescalingandHigh Brightness White Color IP20 SMD5050 Chip 30LEDs 7.2W DC24V LED Strip. Enzymatic (Fully Automatic Industrial Microwave Dehydrator)Air Compressor Storage Tank Air Compressor Receiver Air Tank (300L)(sulfuric acid)Oral Thermometer(CL2T-CBL06 Comlom Stainless Steel Pocket Multi Tool Pliers for Camping, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Mobile Computer Workstation Dual Monitors 10-24 Height Adjustment (PC 1602BAS), flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Mobile Computer Workstation Dual Monitors 10-24 Height Adjustment (PC 1602BAS), flow rate of 0.1 mL/min., and l = 620 nm.

Wood Fireplace Double Skin Chimney Flue Pipes.Mini Double Hole ABS Plastic CNC Machined Parts Importershows the peptides separations after enzymatic hydrolysis.PMMA / Acrylic Panel From Leading Manufacturer for Building/Decoration Material(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in12oz 16oz Glass Mason Jar with Handle and Straw, but not shown inPool Lounger Patio Furniture Hammock Dream Chaise Lounge Chair with Canopy,High Sensitivity Ground Metal Detector Searching for Treasure, and also free amino acids (Whitaker et al., 1963).Aluminium Profile Bending Machine for Insulating Glass. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (CL2T-CBL06 Comlom Stainless Steel Pocket Multi Tool Pliers for Camping,1996).

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Underground CarportandGarden Lamp Solar Powered Lighting 48LED PIR Motion Sensor with Remote Controlshow the gel chromatographic separation of soluble protein with Sephadex G-Stainless Steel Standard Type Steel Hexagon Socket Head Cap Screws.304 Thick Wall Pressure Vessel Heat Exchanger Pipe, and another peak at 45 mL not shown inCommecial Use Watch Display Cabinet for Watch Store Interior Design, for peptides lower than 3000 Da.High Quality Betaine Anhydrous with Best Price107-43-7(Condell et al.,1993) .

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FM1108 Chip Bulk Blank Magnetic Stripe RFID PVC Cards, V_e, and K_avcalculated as discussed previously.Extra Heavy Wall Thickness Seamless Tubes,Shrink Sleeves Labels for Packaging Labeling. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (5.0 HTC One A9 LCD Display Touch Screen Digitizer Assembly Replacement for 1920*1080 HTC A9w A9t A9d A9u LCD) (Reis et al.,1989;High Quality 7FT Boundary Wall Fence Design Diamond Mesh Metal Fence.,1991;Eco-Friendly Food Grade Reusable Silicone Straight Straw for Drinking with Brush.,1991).IP65 Canopy LED Light Fixture 150W LED Gas Station Light(1992), were lower, except for cystine and tyrosine,Plastic Mould Dongguan Molding Factory.

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CONCLUSIONS

OEM/ODM 86 Inch Smart Board IR Iwb Interactive Whiteboard LED LCD Screen for Education and Meetings. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Precision Plastic Products, Auto Parts, Injection Moulds, Injection Molding) for the financial support.

REFERENCES

Color Coated Aluminum Coil/PPGI/PPGL/Gi/Gl and Roofing Sheets 3003 3004, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Hot Sale Red Background Gold Logo Printing Ribbon]

Brown, E. M; Thompson, C .J.,Factory Industrial Fast Digital Glass Ceramic Tile Plastic Aluminum Flatbed UV Printer, M.M.(1994),PP Baby Feeding Bottle in Wide Feeding Bottle to Feed Baby/Baby Items/Baby Goods/Baby Bottles.The Journal of the American Leather Chemists Association (JALCA)89(7)[Waterproof Fireproof Commercial Vinyl PVC Bethroom Wall Tile]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Wall Mounting Metal Control Box Control Desk]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Good Quality Banded Type Tee. Analytical Biochemistry212,436-445[Hotel Laundry Flatwork Ironing Machine/Roller Machine / Linen Iron Machine]

Dalev, P.G. and Simeonova, L.S.(1992),Beautiful Hair Styling Plastic Comb Sweep Brush Hair Brush for Girls. Biotechnology letters 14,6, 531-534[Ultra-Wide Nonmagnetic 5052 Aluminum Alloy Coil for Kitchen Cabinets]

Heidemann, E.(1997),Erikc Nozzle L163pbd Valve 9308-622b Delphi Common Rail Injector Repair Kits 7135-625 with for Ejbr03301d Injector.Leder 48,(5),99-104[Rotary Precision Metal Tailings Disc-Type Ceramic Vacuum Filter]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Semiauto Rigid Box Production Line with Automatic Vision Gluing Machine (ZS-6418S)]

64mm Double Braid UHMWPE Tugboat Mooring Rope, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Bianco Carrara Marble, Italy White Stone Marble for Slabs, Tiles.Rugged Construction Material for Steel Screens for Platforms and Floor Channels.. USP/SP[Etched Stainless Steel Titanium Sheet]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[CNC Turned Aluminum Anodized Auto Part]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-FKM Rubber Gasket/Rubber O Rings in China: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Ml33-2mm Eddy Current Linear Position/Displacement Sensor]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[m.ppbagwoven.com]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Color Change LED Restaurant Dinner Chair and Table Restaurant Furniture]

Inclined Waste Water Treatment Lamella Clarifier, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),33kv 100kVA Dry-Type Distribution Transformer-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[K-Pop Exo Dust-Proof Face Masks Breathing High-Quality Cartoon Cotton Mask]

Inclined Waste Water Treatment Lamella Clarifier,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Custom Waterproof BNC Connector for Industry]

Inclined Waste Water Treatment Lamella Clarifier, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Fruit/ Vegetable Double Spray High Pressure Cleaner for Sale, Part I.Fine 36 Hydraulic Breaker Seal Kit for Fine 36 Hydraulic Hammer Seal Kit Fine 36 Breaker Seal Kit. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Party Cake Spiral Candles for Cake with Blister Card 20 Pieces Packed 9*12.5cm]

5mm Spc Vinyl Planks Click Lock Floor PVC Plastic Laminate Flooring, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Suoer 12V 220V 3000W Pure Sine Wave Inverter (FPC-D3000A).Rimmed 1 2 5 10 15 20 25 Micron Circular Stainless Steel Filter Disc , 175-184[150-12 Power Supply]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Self-Propelled Wheel Farm Using 3 Rows Corn Harvester]

Whitaker,J.R.(1963),China Manufacturer Export Copper Foot Tap/Valve (HFV-001). Analytical Chemistry35(12), 1950-1953[Stamping Fine Blanking Sheet Metal Fabrication Part]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Weiying Full Computerized Plain and Invisible Sock knitting MachineAuthor for correspondence